專利名稱：地衣芽孢桿菌的突變宿主細(xì)胞的制作方法
技術(shù)領(lǐng)域：
芽孢桿菌(Bacillus sp.)是制備異源蛋白的感興趣的宿主，因?yàn)樗鼈兡苤苯訉⒌鞍追置诘脚囵B(yǎng)基中。它們具有高的分泌蛋白的能力，且在遺傳上是高度順從的(amenable)，不致病的(nonpathogenic)，并且無內(nèi)毒素。所以，在芽孢桿菌即在地衣芽孢桿菌(Bacillus licheniformis，B.licheniformis)中已經(jīng)高效制備并分泌了來自不同生物體(organisms)的多種蛋白。
在高度競爭的生物技術(shù)產(chǎn)業(yè)，即使稍微改良(improved)的芽孢桿菌宿主細(xì)胞也是需要的，此宿主細(xì)胞能提供，例如從產(chǎn)品穩(wěn)定性，產(chǎn)品純度等上講，更令人感興趣的生產(chǎn)系統(tǒng)，或甚至只是可選的生產(chǎn)系統(tǒng)。
背景技術(shù)：
芽孢桿菌宿主細(xì)胞內(nèi)可以以生物方式生產(chǎn)很多有商業(yè)效益的工業(yè)產(chǎn)品，例如異源多肽，氨基酸，碳水化合物等。然而，與芽孢桿菌生產(chǎn)型宿主細(xì)胞同源的(native to)污染性(contaminant)多肽也與所述產(chǎn)物一起產(chǎn)生，其中一些可被分泌到所述培養(yǎng)基，且其中一些可保留在細(xì)胞中或保持與細(xì)胞膜相連。此類污染物通常必須被去除(removed)或抑制，來確保例如，所述產(chǎn)物的穩(wěn)定性或儲藏期限(shelf-life)，或用來獲得足夠的產(chǎn)品純度。
在地衣芽孢桿菌中制備異源多肽，尤其會由于存在具有蛋白水解活性的污染性天然多肽而受影響。已經(jīng)采用了不同方式來避開細(xì)胞外降解的問題，包括使編碼已知蛋白酶的基因缺失(Sloma等，1989；Wu等，1991；Sloma等，1991)或在發(fā)酵過程中添加蛋白酶抑制物(Simonen&Pala，1993)。
發(fā)明概述本發(fā)明要解決的問題是，如何提供一種改良的地衣芽孢桿菌宿主細(xì)胞，該細(xì)胞表達(dá)的一或多種具有蛋白水解活性的天然多肽減少。本發(fā)明提供一種解決此問題的辦法，該方法通過鑒定數(shù)個來自地衣芽孢桿菌、具有蛋白水解活性、迄今為止仍未知的多肽，及編碼它們的多核苷酸，來構(gòu)建一個突變體宿主細(xì)胞，其表達(dá)的一或多種這些多肽和/或其緊密相關(guān)的同系物減少。
因此，本發(fā)明一方面涉及，衍生自親代地衣芽孢桿菌宿主細(xì)胞的地衣芽孢桿菌突變體宿主細(xì)胞，所述突變體宿主細(xì)胞在一或多個編碼一或多種具有蛋白水解活性的多肽的基因上有突變，所述多肽與SEQ ID NO2到64(包括SEQ ID NO2和64)，或SEQ ID NO2到SEQ ID NO84(包括SEQ IDNO2和84)所示的一或多種多肽至少80％相同，優(yōu)選至少85％相同，更優(yōu)選至少90％相同，還更優(yōu)選至少95％相同，最優(yōu)選至少97％相同，其中所述突變體宿主細(xì)胞表達(dá)的一或多種具有蛋白水解活性的多肽比在等同條件(comparable conditions)下培養(yǎng)的親代宿主細(xì)胞少至少5％。優(yōu)選所述突變體宿主細(xì)胞表達(dá)的一或多種具有蛋白水解活性的多肽，比在等同條件下培養(yǎng)的親代宿主細(xì)胞少至少10％，更優(yōu)選少至少20％，還更優(yōu)選少至少30％，還更優(yōu)選少至少40％，還更優(yōu)選少至少50％，或少至少60％，或少至少70％，或少至少80％，或最優(yōu)選少至少90％。最優(yōu)選，所述突變體宿主細(xì)胞絕對不表達(dá)一或多種具有蛋白水解活性的多肽。
必須使用等同條件進(jìn)行培養(yǎng)，來比較本發(fā)明的突變體宿主細(xì)胞和親代宿主細(xì)胞中，所述一或多種具有蛋白水解活性多肽的表達(dá)水平。在等同條件下以相同設(shè)置(setup)分別培養(yǎng)它們，當(dāng)然允許通常與生長實(shí)驗(yàn)相關(guān)的操作參數(shù)例如溫度控制等的常規(guī)標(biāo)準(zhǔn)差。所述一或多種多肽的表達(dá)水平的定量是用本領(lǐng)域已知的標(biāo)準(zhǔn)教科書的實(shí)驗(yàn)技術(shù)，例如mRNA定量或基于免疫的(immuno-based)實(shí)驗(yàn)。
本發(fā)明另一方面涉及，一種在地衣芽孢桿菌突變體宿主細(xì)胞中制備至少一種感興趣的產(chǎn)品的方法，其包括在合適的培養(yǎng)基中培養(yǎng)上述方面限定的地衣芽孢桿菌突變體宿主細(xì)胞，在該培養(yǎng)基中產(chǎn)生所述產(chǎn)物。
本發(fā)明最后一方面涉及，第一方面所限定的地衣芽孢桿菌突變體宿主細(xì)胞，在制備至少一種感興趣的產(chǎn)品中的用途，其包含在合適的培養(yǎng)基中培養(yǎng)所述突變體宿主細(xì)胞，在該培養(yǎng)基中產(chǎn)生所述產(chǎn)物。
定義核酸構(gòu)建體(construct)本文所用術(shù)語“核酸構(gòu)建體”指單鏈或雙鏈的核酸分子，其分離自天然存在的基因或被修飾(modified)來以一種天然不存在的方式包含核酸片段(segment)。當(dāng)所述核酸構(gòu)建體包含表達(dá)本發(fā)明的編碼序列所需的控制序列時，該術(shù)語核酸構(gòu)建體與術(shù)語“表達(dá)盒(expression cassette)”同義。
控制序列本文所限定的術(shù)語“控制序列”包括表達(dá)本發(fā)明的多肽所必需的或?qū)ζ溆欣乃薪M分。每個控制序列可以與編碼所述多肽的核苷酸序列同源或異源。此控制序列包括但不限于，前導(dǎo)序列，多腺苷酸化序列(polyadenylation sequence)，前肽序列，啟動子，信號肽序列，和轉(zhuǎn)錄終止子。所述控制序列至少包括啟動子，以及轉(zhuǎn)錄和翻譯終止信號。所述控制序列可以帶接頭，以引入特定限制酶切位點(diǎn)，來促使所述控制序列與編碼多肽的核苷酸序列的編碼區(qū)連接。
可操作地連接本文限定術(shù)語“可操作地連接”為一種構(gòu)型(configuration)，其中控制序列位于與所述DNA序列的編碼序列相關(guān)的適當(dāng)位置，從而所述控制序列可指導(dǎo)多肽的表達(dá)。
編碼序列本文所用術(shù)語“編碼序列”包括核苷酸序列，它直接表明其蛋白產(chǎn)物的氨基酸序列。所述編碼序列的邊界通常由開放閱讀框架來確定，它通常從ATG起始密碼子開始。所述編碼序列通常包括DNA，cDNA和重組的核苷酸序列。
表達(dá)本文上下文中術(shù)語“表達(dá)”包括多肽生產(chǎn)涉及的任何步驟，其包括但不限于轉(zhuǎn)錄、轉(zhuǎn)錄后修飾、翻譯、翻譯后修飾和分泌。
表達(dá)載體本文上下文中術(shù)語“表達(dá)載體”包括線性或環(huán)狀的DNA分子，其包含編碼本發(fā)明多肽的片段，并且該片段可以可操作地連接到使其轉(zhuǎn)錄的其它片段上。

發(fā)明內(nèi)容
一種地衣芽孢桿菌突變體宿主細(xì)胞，其衍生自親代地衣芽孢桿菌宿主細(xì)胞，其中所述突變體宿主細(xì)胞有一或多個基因發(fā)生突變，所述基因編碼一或多種具有蛋白水解活性并且與SEQ ID NO2到SEQ ID NO64(包括SEQ IDNO2和64)，或SEQ ID NO2到SEQ ID NO84(包括SEQ ID NO2和84)中所示的一或多種多肽至少80％相同的多肽，其中所述突變體宿主細(xì)胞表達(dá)的所述一或多種具有蛋白水解活性的多肽，比在等同條件下培養(yǎng)的親代宿主細(xì)胞少至少5％。
本文上下文中術(shù)語“親代宿主細(xì)胞”指，除了在所述突變體的一或多個編碼一或多種具有蛋白水解活性的多肽的突變基因之外，與本發(fā)明子代(progeny)突變體或突變體細(xì)胞在遺傳上(genetically)相同，或等基因的(isogenic)的細(xì)胞。
多肽序列的同一性，或同一性(％)可通過用本領(lǐng)域已知的計算機(jī)程序進(jìn)行序列比對來適當(dāng)?shù)匮芯?，如GCG程序包提供的“GAP”(Program Manual forthe Wisconsin Package，Version 8，August 1994，Genetics Computer Group，575Science Drive，Madison，Wisconsin，USA 53711)(Needleman，S.B.and Wunsch，C.D.，(1970)，Journal of Molecular Biology，48，443-453)。使用如下設(shè)置用GAP來進(jìn)行DNA序列對比GAP產(chǎn)生的罰分為5.0，GAP延伸的罰分為0.3。
本發(fā)明一個目的是提供一種不含天然細(xì)菌蛋白酶的細(xì)菌培養(yǎng)物，來將產(chǎn)品純化降到最小，并得到較好的產(chǎn)品穩(wěn)定性及較高的產(chǎn)品產(chǎn)量。根據(jù)本發(fā)明，這可以通過誘變編碼具有蛋白水解活性的天然多肽的那個或那些基因，來降低或甚至完全消除(abolish)所述一或多種基因的表達(dá)來完成。眾所周知確?；虿槐磉_(dá)成為細(xì)胞內(nèi)的活性多肽的方法之一，簡單來說，是使所述編碼基因缺失或部分缺失。本領(lǐng)域已經(jīng)描述很多關(guān)于如何從細(xì)胞基因組(當(dāng)然包括從地衣芽孢桿菌細(xì)胞的基因組中)特定地缺失或部分缺失一或多個基因的技術(shù)(見例如Novozymes A/S WO 01/90393，Novozymes A/S WO 02/00907)。因此，本發(fā)明優(yōu)選的實(shí)施方案涉及第一方面的宿主細(xì)胞，其通過部分或全部缺失編碼一或多種具有蛋白水解活性的多肽的一或多個基因進(jìn)行突變。
本發(fā)明優(yōu)選的實(shí)施方案涉及第一方面的宿主細(xì)胞，它在編碼兩種或多種具有蛋白水解活性的多肽的兩個或多個基因上發(fā)生突變。
由第一方面的突變體宿主細(xì)胞產(chǎn)生的感興趣的產(chǎn)物，可為一或多個異源基因編碼的一或多種多肽。所以，本發(fā)明優(yōu)選實(shí)施方案涉及第一方面的宿主細(xì)胞，其包括編碼一或多種異源多肽的一或多個異源基因。
在多肽的工業(yè)生產(chǎn)中，需要獲得盡可能高的產(chǎn)物產(chǎn)量。增加產(chǎn)量的一種方式是增加編碼感興趣多肽的基因的拷貝數(shù)。這可以通過將所述基因置于高拷貝數(shù)的質(zhì)粒中來實(shí)現(xiàn)。然而，質(zhì)粒是不穩(wěn)定的，并且如果在宿主細(xì)胞的培養(yǎng)過程中無選擇性壓力，它經(jīng)常從宿主細(xì)胞中丟失。另一種增加所述感興趣基因的拷貝數(shù)的方法，是將其以多拷貝數(shù)整合到所述宿主細(xì)胞的染色體。WO 91/09129和WO 94/14968(Novozymes A/S)，已經(jīng)描述了兩個基因的整合，其內(nèi)容在此引入作為參考。本發(fā)明優(yōu)選的實(shí)施方案涉及第一方面的宿主細(xì)胞，其中所述異源基因存在至少2個拷貝，優(yōu)選至少4個拷貝，最優(yōu)選至少6個拷貝。在另一實(shí)施例中，所述異源基因存在至少十個拷貝。如果由質(zhì)粒攜帶，每個細(xì)胞可存在所述基因的數(shù)百個拷貝，所以在本發(fā)明另一實(shí)施例中，所述異源基因存在至少100個拷貝。
WO 99/41358(Novozymes A/S)(其內(nèi)容在此引入作為參考)已經(jīng)描述了兩個以反向平行串聯(lián)的方式緊密排列的基因進(jìn)行整合，來得到較好的穩(wěn)定性；且WO 02/00907(Novozymes A/S)(其內(nèi)容在此引入作為參考)描述了基因的染色體多拷貝整合。本發(fā)明優(yōu)選實(shí)施方案涉及第一方面的宿主細(xì)胞，其中所述異源基因穩(wěn)定整合到所述細(xì)胞的基因組。
可通過使用如抗生素抗性標(biāo)記物的選擇性標(biāo)記物方便地選擇染色體整合體。然而，理想的是避免使用抗生素抗性標(biāo)記物。WO 01/90393公開一種將基因整合到宿主細(xì)胞的染色體中，而沒有將抗生素抗性標(biāo)記物留在菌株中的方法，其內(nèi)容在此引入作為參考。本發(fā)明優(yōu)選實(shí)施方案涉及第一方面的宿主細(xì)胞，其中所述異源基因被整合到所述細(xì)胞的染色體中，而沒有將任何抗生素抗性標(biāo)記物基因留在整合位點(diǎn)。
本發(fā)明也涉及核酸構(gòu)建體，其包括編碼感興趣產(chǎn)物的核苷酸序列，該核苷酸序列可與一或多個控制序列可操作地連接，此控制序列在與所述控制序列相容的條件下，指導(dǎo)所述編碼序列在適合的宿主細(xì)胞中表達(dá)。
可以以多種方式操縱(manipulate)編碼感興趣多肽的核苷酸序列，來表達(dá)所述多肽。在將所述核苷酸序列插入載體前的操縱可能是理想的或必要的，這取決于表達(dá)載體。用重組DNA方法修飾核苷酸序列的技術(shù)本領(lǐng)域公知。
增加所述產(chǎn)物產(chǎn)量(yield)的其它方法，是提高調(diào)節(jié)特定的感興趣基因的表達(dá)的特定啟動子的啟動子活性。同時，同時更全面地增強(qiáng)數(shù)個啟動子的活性可使產(chǎn)物產(chǎn)量增加。所述控制序列可以是合適的啟動子序列，即被宿主細(xì)胞識別來表達(dá)所述核苷酸序列的一段核苷酸序列。所述啟動子序列包含轉(zhuǎn)錄控制序列，它介導(dǎo)所述多肽的表達(dá)。所述啟動子可以是任何在選定宿主細(xì)胞內(nèi)顯示轉(zhuǎn)錄活性的核苷酸序列，其包括突變體的，截短的，和雜合的啟動子，并且可從編碼細(xì)胞外或細(xì)胞內(nèi)多肽、與所述宿主細(xì)胞同源或異源的基因獲得。
指導(dǎo)本發(fā)明核酸構(gòu)建體(特別是在細(xì)菌宿主細(xì)胞中)轉(zhuǎn)錄的合適啟動子的例子，為得自如下的啟動子大腸桿菌lac操縱子，天藍(lán)色鏈霉菌(Streptomyces coelicolor)的瓊脂糖酶基因(dagA)，枯草芽孢桿菌(Bacillussubtilis)的果聚糖蔗糖酶(levansucrase)基因(sacB)，地衣芽孢桿菌的α-淀粉酶基因(amyL)，嗜熱脂肪芽孢桿菌(Bacillus stearothermophilus)的麥芽糖(maltogenic)淀粉酶基因(amyM)，解淀粉芽孢桿菌(Bacillus amyloliquefaciens)的α-淀粉酶基因(amyQ)，地衣芽孢桿菌的青霉素酶基因(penP)，枯草芽孢桿菌的xylA和xylB基因，和原核生物的β-內(nèi)酰胺酶基因(Villa-Kamaroff等，1978，Proceedings of the National Academy of Sciences USA 753727-3731)，以及tac啟動子(DeBoer等，1983，Proceedings of the National Academy ofSciences USA8021-25)。更多啟動子見“Useful proteins from recombinantbacteria”in Scientific American，1980，24274-94；和Sambrook等，1989(見上文)所述。
其它可用的啟動子見WO93/10249，WO 98/07846和WO 99/43835(Novozymes A/S)所述，其全部內(nèi)容在此引入作為參考。本發(fā)明優(yōu)選實(shí)施方案涉及第一方面的宿主細(xì)胞，其中所述異源基因從異源啟動子或人工啟動子轉(zhuǎn)錄。
所述控制序列也可為適合的轉(zhuǎn)錄終止子序列，即一種為宿主細(xì)胞識別來終止轉(zhuǎn)錄的序列。所述終止子序列可操作地連接于編碼所述多肽的核苷酸序列的3′末端。任何在所選的宿主細(xì)胞中起作用的終止子序列都可用于本發(fā)明。
所述控制序列也可為適合的前導(dǎo)序列，mRNA的非翻譯區(qū)，它對于被所述宿主細(xì)胞翻譯是重要的。所述前導(dǎo)序列可操作地連接于編碼所述多肽的核苷酸序列的5′末端。任何在所選的宿主細(xì)胞中起作用的前導(dǎo)序列都可用于本發(fā)明。
所述控制序列也可為多腺苷酸化序列，即一種可操作性地連接于所述核苷酸序列的3′末端的序列，并且在轉(zhuǎn)錄時它作為所述宿主細(xì)胞識別的信號，來將多腺苷殘基加到轉(zhuǎn)錄的mRNA上。任何在所選的宿主細(xì)胞起作用的多腺苷酸化序列都可用于本發(fā)明。
所述控制序列也可為信號肽編碼區(qū)，其編碼與多肽的氨基末端連接的氨基酸序列，并指導(dǎo)被編碼的多肽進(jìn)入所述細(xì)胞分泌途徑。所述核苷酸序列的編碼序列的5′末端可先天(inherently)包含信號肽編碼區(qū)，該信號肽的編碼區(qū)在翻譯閱讀框中與編碼所述分泌多肽的編碼區(qū)片段天然相連?；蛘?，所述編碼序列的5′末端可包含與所述編碼序列異源的信號肽編碼區(qū)。當(dāng)所述編碼序列不天然地包含信號肽編碼區(qū)時，可能需要異源的信號肽編碼區(qū)?；蛘?，可用所述異源的信號肽編碼區(qū)簡單地置換天然的信號肽編碼區(qū)，來提高所述多肽的分泌。然而，任何指導(dǎo)表達(dá)的多肽進(jìn)入所選宿主細(xì)胞的分泌途徑的信號肽編碼區(qū)，都可用于本發(fā)明。
細(xì)菌宿主細(xì)胞的有效信號肽編碼區(qū)是得自如下物質(zhì)的基因的信號肽編碼區(qū)芽孢桿菌NCIB 11837的麥芽糖淀粉酶，嗜熱脂肪芽孢桿菌的α-淀粉酶，地衣芽孢桿菌枯草桿菌蛋白酶(subtilisin)，地衣芽孢桿菌的β-內(nèi)酰胺酶，嗜熱脂肪芽孢桿菌的中性蛋白酶(nprT，nprS，nprM)，和枯草芽孢桿菌prsA。更多信號肽見Simonen和Palva，1993，Microbiological Reviews 57109-137所述。
所述控制序列也可為編碼位于多肽氨基末端的氨基酸序列的前肽編碼區(qū)。所得多肽稱為酶原(proenzyme)或多肽原(propolypeptide)(或一些情況下酶原(zymogen))。多肽原通常是無活性的，并能通過從多肽原催化或自動催化切割所述前肽而轉(zhuǎn)化為成熟的活性多肽。所述前肽編碼區(qū)可得自如下物質(zhì)的基因枯草芽孢桿菌的堿性蛋白酶(aprE)，枯草芽孢桿菌的中性蛋白酶(nprT)，釀酒酵母(Saccharomyces cerevisiae)的α-因子，米赫根毛霉(Rhizomucor miehei)的天冬氨酸蛋白酶，和嗜熱毀絲酶(Myceliophthorathermophila)的漆酶(laccase)(WO 95/33836)。
當(dāng)信號肽和前肽區(qū)存在于多肽的氨基末端時，所述前肽區(qū)位于所述多肽的氨基末端旁，并且所述信號肽區(qū)位于所述前肽區(qū)的氨基末端旁。
理想的是，加入調(diào)控與所述宿主細(xì)胞生長相關(guān)的多肽的表達(dá)的調(diào)控序列。所述調(diào)控系統(tǒng)的實(shí)例是，應(yīng)化學(xué)或物理刺激(包括存在調(diào)控化合物)，打開或關(guān)閉(turned on or off)所述基因的表達(dá)的那些調(diào)控系統(tǒng)。原核生物系統(tǒng)中的調(diào)控系統(tǒng)包括lac，tac，和trp操縱子系統(tǒng)。酵母中，可用ADH2系統(tǒng)或GAL1系統(tǒng)。真核生物系統(tǒng)中，調(diào)控系統(tǒng)包括二氫葉酸還原酶基因(它在甲氨喋呤存在時擴(kuò)增)和金屬硫蛋白基因(它們隨重金屬擴(kuò)增)。此情況下，編碼所述多肽的核苷酸序列可與調(diào)控序列可操作地連接。
本發(fā)明也涉及包含本發(fā)明核酸構(gòu)建體的重組表達(dá)載體。上述多種核苷酸和控制序列可相互連接(joined together)來產(chǎn)生重組表達(dá)載體，該載體包括一或多個方便的限制性位點(diǎn)，使得可在所述位點(diǎn)插入或替代編碼所述多肽的核苷酸序列。或者，可通過將所述核苷酸序列或包含所述序列的核酸構(gòu)建體插入合適的表達(dá)載體，來表達(dá)本發(fā)明所述核苷酸序列。創(chuàng)建所述表達(dá)載體時，所述編碼序列位于載體中，從而使得編碼序列可操作地連接到合適的表達(dá)控制序列。
所述重組表達(dá)載體可以是任何載體(例如，質(zhì)?；虿《?，它可以方便地用重組DNA方法處理，并可表達(dá)所述核苷酸序列。選擇載體通常取決于所述載體與所述載體被引入的宿主細(xì)胞的相容性。所述載體可以是線性的或閉合環(huán)狀質(zhì)粒。
所述載體可以是自主復(fù)制載體，即作為染色體外實(shí)體存在的載體，其復(fù)制不依賴于染色體的復(fù)制，例如，質(zhì)粒，染色體外元件，微型染色體，或人工染色體的復(fù)制。
所述載體可包含任何確保自身復(fù)制的工具(means)。或者，所述載體可以在引入所述宿主細(xì)胞時，被整合進(jìn)入基因組并與它所整合進(jìn)入的染色體一起復(fù)制。另外，可用單個載體或質(zhì)粒，或者總共包含要引入所述宿主細(xì)胞的基因組的全部DNA的兩個或多個載體或質(zhì)粒，或可用轉(zhuǎn)座子。
本發(fā)明所述載體優(yōu)選包含一或多個選擇性標(biāo)記物，其可使選擇轉(zhuǎn)化的細(xì)胞變得容易。選擇性標(biāo)記物是一種基因，其產(chǎn)物提供殺生物劑(biocide)或病毒抗性，重金屬抗性，營養(yǎng)缺陷體的原養(yǎng)型(prototrophy to auxotroph)等等。
細(xì)菌的選擇性標(biāo)記物實(shí)例是枯草芽孢桿菌的dal基因或地衣芽孢桿菌的dal基因，或帶來抗生素抗性，如氨芐青霉素，卡那霉素，氯霉素或四環(huán)素抗性的標(biāo)記物。
本發(fā)明所述載體優(yōu)選包含一種元件，它使所述載體穩(wěn)定整合到所述宿主細(xì)胞的基因組，或使所述載體在細(xì)胞中不依賴于基因組而自主地復(fù)制。
為整合進(jìn)入所述宿主細(xì)胞基因組，所述載體可依賴于編碼所述多肽的核苷酸序列，或使所述載體通過同源或非同源重組穩(wěn)定地整合到基因組的任何其它載體元件?；蛘?，所述載體可包含附加的核苷酸序列，來指導(dǎo)通過同源重組整合到所述宿主細(xì)胞的基因組。該附加的核苷酸序列使所述載體被整合到染色體中所述宿主細(xì)胞基因組的精確位置。為提高整合到精確位點(diǎn)的可能性，所述整合元件應(yīng)優(yōu)選包含足夠數(shù)量的核苷酸，如100到1,500個堿基對，優(yōu)選400到1,500個堿基對，最優(yōu)選800到1,500個堿基對，它與對應(yīng)的靶序列高度同源，從而提高同源重組的可能性。所述整合元件可為任何與所述宿主細(xì)胞基因組中的靶序列同源的序列。另外，所述整合元件可為非編碼型或編碼型核苷酸序列。另一方面，所述載體可通過非同源重組，整合到所述宿主細(xì)胞的基因組。
對于自主復(fù)制，所述載體還可包含復(fù)制起點(diǎn)，它使所述載體在目的宿主細(xì)胞中自主復(fù)制。細(xì)菌復(fù)制起點(diǎn)的實(shí)例是質(zhì)粒pBR322，pUC19，pACYC177和pACYC184的復(fù)制起點(diǎn)(可在大腸桿菌中復(fù)制)和質(zhì)粒pUB110，pE194，pTA1060和pAMss1的復(fù)制起點(diǎn)(可在芽孢桿菌中復(fù)制)。所述復(fù)制起點(diǎn)可以有突變以使其在宿主細(xì)胞內(nèi)的功能是熱敏的(temperature-sensitive)(見例如Ehrlich，1978，Proceedings of the National Academy of Sciences USA 751433)。
本發(fā)明核苷酸序列的一個以上的拷貝，可被插入所述宿主細(xì)胞，來提高所述基因產(chǎn)物的產(chǎn)出(product)。增加所述核苷酸序列的拷貝數(shù)，可以通過如下方式獲得將所述序列的至少一個另外的拷貝整合到所述宿主細(xì)胞基因組，或如果細(xì)胞含有擴(kuò)增的拷貝的選擇性標(biāo)記基因，可通過將一個擴(kuò)增的(amplifiable)選擇性標(biāo)記物基因并入(included)所述核苷酸序列中，從而可以通過在存在適當(dāng)?shù)倪x擇試劑時培養(yǎng)所述細(xì)胞，來選擇所述核苷酸序列的額外拷貝。
用來連接上述元件來構(gòu)建本發(fā)明重組表達(dá)載體的方法對本領(lǐng)域技術(shù)人員公知(見例如Sambrook等，1989，見上文)。
將載體引入細(xì)菌宿主細(xì)胞，可例如，用原生質(zhì)體轉(zhuǎn)化(見例如，Chang和Cohen，1979，Molecular General Genetics 168111-115)，用感受態(tài)細(xì)胞(見例如，Young和Spizizin，1961，Journal of Bacteriology 81823-829，或Dubnau和Davidoff-Abelson，1971，Journal of Molecular Biology 56209-221)，電穿孔(見例如，Shigekawa和Dower，1988，Biotechniques 6742-751)，或連接法(conjugation)(見例如，Koehler和Thorne，1987，Journal of Bacteriology 1695771-5278)來進(jìn)行。
本發(fā)明優(yōu)選實(shí)施方案涉及第一方面的宿主細(xì)胞，其中所述異源基因被包含在操縱子，優(yōu)選多順反子操縱子中。本發(fā)明上下文中的術(shù)語“操縱子”指包含數(shù)個基因的多核苷酸，所述基因呈聚簇(cluster)并可能甚至一起轉(zhuǎn)錄成為多順反子mRNA，例如編碼代謝途徑的酶的基因。所述操縱子的轉(zhuǎn)錄可從啟動子區(qū)域開始，并受臨近編碼調(diào)控蛋白的調(diào)控基因的控制，所述調(diào)控蛋白反過來結(jié)合到所述操縱子的操縱序列來分別抑制或增強(qiáng)轉(zhuǎn)錄。此基因或操縱子可被攜帶在可穩(wěn)定保留的(maintained)的合適質(zhì)粒上，例如，能在宿主細(xì)胞內(nèi)穩(wěn)定地自我復(fù)制(質(zhì)粒的選擇通常依賴于所述質(zhì)粒與該質(zhì)粒被引入的宿主細(xì)胞的相容性)，或者所述基因或操縱子可被攜帶在所述宿主的染色體上。所述基因可以是宿主細(xì)胞內(nèi)源的，在這種情況下，該感興趣的產(chǎn)物是由所述宿主細(xì)胞天然產(chǎn)生的蛋白，并且大多情況下，該基因會處于染色體上的正常位置。如果編碼感興趣產(chǎn)物的所述基因是外源基因，該基因可由適當(dāng)?shù)馁|(zhì)粒攜帶，或者被整合到宿主染色體上。本發(fā)明一個實(shí)施方案中，真細(xì)菌(eubacterium)是重組的真細(xì)菌。另一個實(shí)施方案中，所述感興趣的產(chǎn)物也可為重組蛋白。
所述感興趣的產(chǎn)物是任何基因產(chǎn)物或代謝途徑的產(chǎn)物，它是工業(yè)上可用的，并可在細(xì)菌細(xì)胞如地衣芽孢桿菌中制備。
一個優(yōu)選實(shí)施方案中，所述異源多肽是抗微生物肽或融合肽，該融合肽包含其天然形式具有抗微生物活性的肽部分。
另一個優(yōu)選實(shí)施方案中，所述異源多肽具有生物合成的活性，并產(chǎn)生感興趣的化合物或中間體(intermediate)。
另一個實(shí)施方案涉及第一方面的宿主細(xì)胞，其中所述感興趣的化合物或中間體包含維生素，氨基酸，抗生素，碳水化合物，或表面活性劑，并優(yōu)選所述碳水化合物包含透明質(zhì)酸。
一個優(yōu)選實(shí)施方案中，所述異源多肽是酶，所述酶特別是選自如下酶類的酶氧化還原酶(EC 1)，轉(zhuǎn)移酶(EC 2)，水解酶(EC 3)，裂解酶(EC 4)，異構(gòu)酶(EC 5)，和連接酶(EC 6)。優(yōu)選所述酶是具有選自如下酶的活性氨肽酶，淀粉酶，淀粉葡萄糖苷酶，甘露聚糖酶，糖酶，羧肽酶，過氧化氫酶；纖維素酶，幾丁質(zhì)酶(chitinase)，角質(zhì)酶，環(huán)糊精糖基轉(zhuǎn)移酶(cyclodextringlycosyltransferase)，脫氧核糖核酸酶，酯酶，半乳糖苷酶，β-半乳糖苷酶，葡糖淀粉酶，葡萄糖氧化酶，葡糖苷酶，鹵過氧化物酶(haloperoxidase)，半纖維素酶，轉(zhuǎn)化酶，異構(gòu)酶，漆酶，連接酶，脂酶，裂合酶，甘露糖苷酶，氧化酶，果膠酶，過氧化物酶，肌醇六磷酸酶(phytase)，酚氧化酶，多酚氧化酶，蛋白酶，核糖核酸酶，轉(zhuǎn)移酶，轉(zhuǎn)谷氨酰胺酶(transglutaminase)和木聚糖酶。優(yōu)選所述酶是淀粉酶或甘露聚糖酶。
本發(fā)明的第二方面涉及在地衣芽孢桿菌突變體宿主細(xì)胞中制備至少一種感興趣產(chǎn)物的方法，包含在合適的培養(yǎng)基中培養(yǎng)本發(fā)明第一方面所限定的地衣芽孢桿菌突變體宿主細(xì)胞，從其中制備了所述產(chǎn)物。一個實(shí)施方案涉及第二方面的方法，其還包含分離或純化所述感興趣的產(chǎn)物。適合培養(yǎng)的培養(yǎng)基描述如下，并且純化或分離所制備的產(chǎn)物的方法，是本發(fā)明方法的可選附加步驟。
本發(fā)明制備方法中，所述細(xì)胞在適合制備所述多肽的營養(yǎng)培養(yǎng)基中、用本領(lǐng)域已知方法進(jìn)行培養(yǎng)。例如，所述細(xì)胞可通過搖瓶(shake flask)培養(yǎng)，小規(guī)?；虼笠?guī)模發(fā)酵(包括連續(xù)、批量(batch)、分批(fed-batch)、或固態(tài)發(fā)酵)在實(shí)驗(yàn)室或工業(yè)發(fā)酵罐中，在合適的培養(yǎng)基內(nèi)和使所述多肽表達(dá)和/或分離的條件下進(jìn)行此培養(yǎng)。所述培養(yǎng)在合適的營養(yǎng)培養(yǎng)基中用本領(lǐng)域已知的方法進(jìn)行，該培養(yǎng)基包含碳和氮源以及無機(jī)鹽。合適的培養(yǎng)基來自供應(yīng)商(commercial suppliers)或可根據(jù)公開的組合物制備(例如美國典型培養(yǎng)物保藏中心(the American Type Culture Collection)的目錄)。如果所述多肽被分泌到所述營養(yǎng)培養(yǎng)基中，可直接從所述培養(yǎng)基回收此多肽。如果所述多肽不被分泌，可從細(xì)胞裂解物回收它。
用于培養(yǎng)所述細(xì)胞的培養(yǎng)基可為任何常用的適合所述宿主細(xì)胞生長的培養(yǎng)基，如極限培養(yǎng)基(minimal medium)或復(fù)合(complex)培養(yǎng)基，其包含合適的補(bǔ)充物(supplement)。合適的培養(yǎng)基來自供應(yīng)商或可根據(jù)公開的配方(recipes)制備(例如美國典型培養(yǎng)物保藏中心的目錄)。此培養(yǎng)基用本領(lǐng)域已知的方法制備(見例如，細(xì)菌和酵母的參考書(references)，Bennett，J.W.和LaSure，L.，editors，More Gene Manipulations in Fungi，Academic Press，CA，199l)。
此多肽可用本領(lǐng)域已知對此多肽特定的方法來檢測。這些檢測方法可包括使用特定的抗體，形成酶產(chǎn)物，或酶底物的消失。例如，可用酶試驗(yàn)(assay)來確定本文所述多肽的活性。
所得多肽可用本領(lǐng)域已知的方法來回收。例如，可用常用方法，包括但不限于，離心、過濾、提取、噴霧干燥(spray-drying)、蒸發(fā)或沉淀，從營養(yǎng)培養(yǎng)基來回收所述多肽。
本發(fā)明多肽可用多種本領(lǐng)域已知方法純化，所述方法包括但不限于，層析(例如，離子交換層析、親合層析、疏水層析、層析聚焦(chromatofocusing)和體積排除色譜(size exclusion))，電泳方法(例如制備型等電聚焦(preparativeisoelectric focusing))，差異溶解度(differential solubility)(例如，硫酸銨沉淀)，SDS-PAGE，或提取(見例如，Protein Purification，J.-C.Janson和LarsRyden，editors，VCH Publishers，New York，1989)。
本發(fā)明第三方面涉及第一方面所限定的地衣芽孢桿菌突變體宿主細(xì)胞，在制備至少一種感興趣的產(chǎn)物中的用途，其包括在合適培養(yǎng)基中培養(yǎng)所述突變體宿主細(xì)胞，從而制備所述產(chǎn)物，并可選分離或純化所制備的產(chǎn)物。
序列表<110>諾維信公司(Novozymes A/S)邁克爾.D.拉斯馬森斯蒂恩.T.喬根森彼得.B.奧爾森詹斯.T.安德森<120>改良的芽孢桿菌宿主細(xì)胞<130>10196.204<160>84<170>PatentIn version 3.2<210>1<211>4325<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(3)..(3836)<400>1tg gaa caa gta gcg gct ttt ccg gaa gta gaa aaa gtg ctt ccg aac 47Glu Gln Val Ala Ala Phe Pro Glu Val Glu Lys Val Leu Pro Asn1 5 10 15gaa aaa agg cag ctg atc aaa ccg act aaa cag tct gtg aaa aaa tca95G1u Lys Arg Gln Leu Ile Lys Pro Thr Lys Gln Ser Val Lys Lys Ser20 25 30gca gcc aag gat gaa aaa gaa atc gaa tgg aat atc aat cga gtc gac143Ala Ala Lys Asp Glu Lys Glu Ile Glu Trp Asn Ile Asn Arg Val Asp35 40 45gca cca aaa gcc tgg aaa ttg gga tat gac gga tcg ggc acg gtt gtc191Ala Pro Lys Ala Trp Lys Leu Gly Tyr Asp Gly Ser Gly Thr Val Val50 55 60gcg tcg att gat acg ggc gtg cag tgg gat cat ccg gcc ttg aaa gaa239Ala Ser Ile Asp Thr Gly Val Gln Trp Asp His Pro Ala Leu Lys Glu65 70 75aaa tac cgc gga tat gac ccg cag cat ccc gat cag cct aac cac gaa287Lys Tyr Arg Gly Tyr Asp Pro Gln His Pro Asp Gln Pro Asn His Glu80 85 90 95ttc agt tgg tat gat gcg gtt tca ggc gct tct gaa cca ttt gac gat335Phe Ser Trp Tyr Asp Ala Val Ser Gly Ala Ser Glu Pro Phe Asp Asp100 105 110ctg gag cac ggc acg cat gta acc ggg acg atg gtc ggc tca gag cct383Leu Glu His Gly Thr His Val Thr Gly Thr Met Val Gly Ser Glu Pro115 120 125gac ggc caa aac caa atc ggc gtg gcg ccg ggt gcg aag tgg att gct431Asp Gly Gln Asn Gln Ile Gly Val Ala Pro Gly Ala Lys Trp Ile Ala130 135 140gtg aaa gct ttt tca gat gac ggc ggt acg gac gaa gac ctt att gca479Val Lys Ala Phe Ser Asp Asp Gly Gly Thr Asp Glu Asp Leu Ile Ala145 150 155gca ggt gaa tgg att ttg gcg cca aaa gat aaa gac ggc aac ccg cac527Ala Gly Glu Trp Ile Leu Ala Pro Lys Asp Lys Asp Gly Asn Pro His
160 165 170 175cct gaa atg gcg ccg gat gtc gtc aat aac tca tgg tca ggg gga gcc 575Pro Glu Met Ala Pro Asp Val Val Asn Asn Ser Trp Ser Gly Gly Ala180 185 190ggg att gat gag ttt tac agg gat atc gta aaa gcg tgg cga gct gcc 623Gly Ile Asp Glu Phe Tyr Arg Asp Ile Val Lys Ala Trp Arg Ala Ala195 200 205ggc atc ttt cct gag ttc tct gcg ggg aac gtc gat ttc gcc aat ccg 671Gly Ile Phe Pro Glu Phe Ser Ala Gly Asn Val Asp Phe Ala Asn Pro210 215 220gga gga cct gct tcg att gcc aat ccg gca aac tac cct gaa gcg ttc 719Gly Gly Pro Ala Ser Ile Ala Asn Pro Ala Asn Tyr Pro Glu Ala Phe225 230 235gca acc gga gcg aca gac atc gat aat cgc tta gcc gat ttt tca ctt 767Ala Thr Gly Ala Thr Asp Ile Asp Asn Arg Leu Ala Asp Phe Ser Leu240 245 250 255caa ggt cct tcc ccg tat gat gag act aag cct gaa ata tcg gcg cct 815Gln Gly Pro Ser Pro Tyr Asp Glu Thr Lys Pro Glu Ile Ser Ala Pro260 265 270ggt gtc aac att cgc tca tct gtg cct gga agc ggc tat caa gac ggc 863Gly Val Asn Ile Arg Ser Ser Val Pro Gly Ser Gly Tyr Gln Asp Gly275 280 285tgg gac ggc act tca atg gct ggc ccg cat gtc gca gcc gcc gcc gct 911Trp Asp Gly Thr Ser Met Ala Gly Pro His Val Ala Ala Ala Ala Ala290 295 300ctt atc aag caa gcc gat tcg tcg atc act gtt gat gag acc gaa aag 959Leu Ile Lys Gln Ala Asp Ser Ser Ile Thr Val Asp Glu Thr Glu Lys305 310 315att ttg atg gaa act gca aca ccg ctc aca gac agc aaa ttt acc gaa1007Ile Leu Met Glu Thr Ala Thr Pro Leu Thr Asp Ser Lys Phe Thr Glu320 325 330 335tcg ccg aac aac gga tac ggc cac gga ctc gtg aac gtg ttt gac gca1055Ser Pro Asn Asn Gly Tyr Gly His Gly Leu Val Asn Val Phe Asp Ala340 345 350gtg tcc gct gtg aca gac ggg ttg ggc aga gcg gaa gga caa gtc gga1103Val Ser Ala Val Thr Asp Gly Leu Gly Arg Ala Glu Gly Gln Val Gly355 360 365aaa gaa ggc gaa gac aaa agt ccg ccg gcc tta aac cat caa gag atc1151Lys Glu Gly Glu Asp Lys Ser Pro Pro Ala Leu Asn His Gln Glu Ile370 375 380acc gaa att tac tca ggc tcg gaa acg cct ctt aaa gct gat gta gaa1199Thr Glu Ile Tyr Ser Gly Ser Glu Thr Pro Leu Lys Ala Asp Val Glu385 390 395gac gat gtc agc atc ata tcg gtc aaa ttg agc tat aaa aca gat gac1247Asp Asp Val Ser Ile Ile Ser Val Lys Leu Ser Tyr Lys Thr Asp Asp400 405 410 415gcc gat tgg gag acg atc gct gcc aaa caa act tcc ggc gac tat aaa1295Ala Asp Trp Glu Thr Ile Ala Ala Lys Gln Thr Ser Gly Asp Tyr Lys420 425 430aaa gga acg tac gag gcg gct gta act gtt tct gaa ggg aaa acc ctt1343Lys Gly Thr Tyr Glu Ala Ala Val Thr Val Ser Glu Gly Lys Thr Leu435 440 445
tcc tac aag tgg att gtc acc gat ttc gga ggc aac aaa acg gaa tcc1391Ser Tyr Lys Trp Ile Val Thr Asp Phe Gly Gly Asn Lys Thr Glu Ser450 455 460aaa gtg tac gaa gtc ccg att tcc ccg gct gtg aca aca ggg tat aaa1439Lys Val Tyr Glu Val Pro Ile Ser Pro Ala Val Thr Thr Gly Tyr Lys465 470 475caa gat ttc gag aat tcc gcc agc ggc tgg ctc aca tca ggc gtg aag1487Gln Asp Phe Glu Asn Ser Ala Ser Gly Trp Leu Thr Ser Gly Val Lys480 485 490 495aat tca tgg gag cgc gga att cct cag tca ggc ccg aac gcc gca gca1535Asn Ser Trp Glu Arg Gly Ile Pro Gln Ser Gly Pro Asn Ala Ala Ala500 505 510tcg gga aaa aac gta ttc gca aca aat ctg aca ggg ccg tat gaa agt1583Ser Gly Lys Asn Val Phe Ala Thr Asn Leu Thr Gly Pro Tyr Glu Ser515 520 525tcg gct aat atg aat ctt ctc atg cct cct gtc agc gtt cca aaa aat1631Ser Ala Asn Met Asn Leu Leu Met Pro Pro Val Ser Val Pro Lys Asn530 535 540caa aag ctt tat ctc aca tac aaa tat tgg cgc gat att gaa gag gac1679Gln Lys Leu Tyr Leu Thr Tyr Lys Tyr Trp Arg Asp Ile Glu Glu Asp545 550 555ttt gat tac ggc ttc gtt tac gta cag cct gaa gga aaa ggt gaa tgg1727Phe Asp Tyr Gly Phe Val Tyr Val Gln Pro Glu Gly Lys Gly Glu Trp560 565 570 575atc ccg gct gcc gag tac agc ggc aag aca tca gaa tgg aaa gac gga1775Ile Pro Ala Ala Glu Tyr Ser Gly Lys Thr Ser Glu Trp Lys Asp Gly580 585 590cag atc gat ttg tcg gaa tac ggg gga cag acg att aag gtc atg ttc1823Gln Ile Asp Leu Ser Glu Tyr Gly Gly Gln Thr Ile Lys Val Met Phe595 600 605aac ctt caa tct gac gac agc att gaa ggt gac ggg ctg tac atc gat1871Asn Leu Gln Ser Asp Asp Ser Ile Glu Gly Asp Gly Leu Tyr Ile Asp610 615 620gat gta gcg ctt gtc aag gaa gtg aag agc gcc ggt acg aaa aaa cga1919Asp Val Ala Leu Val Lys Glu Val Lys Ser Ala Gly Thr Lys Lys Arg625 630 635ttg ggc gtt gaa aaa cag ccg gcc aaa atg aag gat aag aaa aca aag1967Leu Gly Val Glu Lys Gln Pro Ala Lys Met Lys Asp Lys Lys Thr Lys640 645 650 655aaa cga atg att gat ccg aaa aaa gca aaa ccg gca gaa gcc ctt cag2015Lys Arg Met Ile Asp Pro Lys Lys Ala Lys Pro Ala Glu Ala Leu Gln660 665 670gaa aaa acc gaa acg aaa aaa gca gct cct gcg gtt ctt ccg gtg cgc2063Glu Lys Thr Glu Thr Lys Lys Ala Ala Pro Ala Val Leu Pro Val Arg675 680 685gct cag gtc agc gtg ctg gaa acg ggc aaa tcg aca tat tcc aac cag2111Ala Gln Val Ser Val Leu Glu Thr Gly Lys Ser Thr Tyr Ser Asn Gln690 695 700gca aca ggc gca tac agc ttg gct cat gca cct gga acc tat acg ctg2159Ala Thr Gly Ala Tyr Ser Leu Ala His Ala Pro Gly Thr Tyr Thr Leu705 710 715
aaa gcg gaa gcg tac gga tat gaa tca acc gcc caa acc gta aaa atc2207Lys Ala Glu Ala Tyr Gly Tyr Glu Ser Thr Ala Gln Thr Val Lys Ile720 725 730 735gag tcc gac aag aca aca acg gca gac ttt gtt tta aaa gaa cta aaa2255Glu Ser Asp Lys Thr Thr Thr Ala Asp Phe Val Leu Lys Glu Leu Lys740 745 750aaa ggg acg ctc acc gga acg ata aaa aat aaa aaa acg ggc gaa ccc2303Lys Gly Thr Leu Thr Gly Thr Ile Lys Asn Lys Lys Thr Gly Glu Pro755 760 765gtc cgg cat gca aag ttg tat atc gtt gaa gat gca gct gta aaa cca2351Val Arg His Ala Lys Leu Tyr Ile Val Glu Asp Ala Ala Val Lys Pro770 775 780gtc cag acg gat gat gac gga agc tat tca tta acg gcc tat gaa ggc2399Val Gln Thr Asp Asp Asp Gly Ser Tyr Ser Leu Thr Ala Tyr Glu Gly785 790 795tcg tat acg gta aag gtc tct gca aac gga tat tac agc agc gag ttt2447Ser Tyr Thr Val Lys Val Ser Ala Asn Gly Tyr Tyr Ser Ser Glu Phe800 805 810 815tcc gtt gat tta aaa ggc gat gtc tcg aaa gat atc gat ctc gat cct2495Ser Val Asp Leu Lys Gly Asp Val Ser Lys Asp Ile Asp Leu Asp Pro820 825 830ttc atc ggc tat ccg ggg gaa ata ggc tat gat gac gga acc ggg gaa2543Phe Ile Gly Tyr Pro Gly Glu Ile Gly Tyr Asp Asp Gly Thr Gly Glu835 840 845aac gcc tgg gct ttc tat gaa tcc ggc aac ggg ctg gcg gtt aaa atg2591Asn Ala Trp Ala Phe Tyr Glu Ser Gly Asn Gly Leu Ala Val Lys Met850 855 860acg ctt gaa aac ggg cag gaa aaa gcc atg ctg aaa ggc ggt ttg ttt2639Thr Leu Glu Asn Gly Gln Glu Lys Ala Met Leu Lys Gly Gly Leu Phe865 870 875aaa ttc tgg gat aca gaa ttc cct gat ccg ggc ggc act gat ttt gca2687Lys Phe Trp Asp Thr Glu Phe Pro Asp Pro Gly Gly Thr Asp Phe Ala880 885 890 895gta gag gtc tat gat gct tcg ggt gaa aaa ggc tct ccg ggc aaa aaa2735Val Glu Val Tyr Asp Ala Ser Gly Glu Lys Gly Ser Pro Gly Lys Lys900 905 910atc gcc ggc ccg ttc aag gct gaa gcg ctc cgc aca ggc gaa tgg acg2783Ile Ala Gly Pro Phe Lys Ala Glu Ala Leu Arg Thr Gly Glu Trp Thr915 920 925acc gtt gat tta ggt gac gaa ggc atc atc gtc gga aaa gac ttt tac2831Thr Val Asp Leu Gly Asp Glu Gly Ile Ile Val Gly Lys Asp Phe Tyr930 935 940ctc gta tac gtc cag aaa gag gat ctg gcc aac tca cct gga ctt gct2879Leu Val Tyr Val Gln Lys Glu Asp Leu Ala Asn Ser Pro Gly Leu Ala945 950 955aca gac gaa gac gga gaa tat tcc ggc cgc aac tgg cag tat acg gac2927Thr Asp Glu Asp Gly Glu Tyr Ser Gly Arg Asn Trp Gln Tyr Thr Asp960 965 970 975ggc tca tgg tca aaa gcg cca tca gac caa ggc aac ttt atg atc cgc2975Gly Ser Trp Ser Lys Ala Pro Ser Asp Gln Gly Asn Phe Met Ile Arg980 985 990gct ttg gtc gac tat gaa ttg tcg gtg cct gtg att acg tct cct aaa3023
Ala Leu Val Asp Tyr Glu Leu Ser Val Pro Val Ile Thr Ser Pro Lys995 10001005gac gga ttc atc acc aac caa aag aac gct gtc att gaa ggg aca3068Asp Gly Phe Ile Thr Asn Gln Lys Asn Ala Val Ile Glu Gly Thr101010151020tcc tcg ccg aat acg acc gtt cat ctc ttt aac ggg gat gaa gaa3113Ser Ser Pro Asn Thr Thr Val His Leu Phe Asn Gly Asp G1u Glu102510301035gcc gga aca gcg gaa acg gcg gca gac ggc act ttc tca aaa gaa3158Ala Gly Thr Ala Glu Thr Ala Ala Asp Gly Thr Phe Ser Lys Glu104010451050ata ccg ttg aat aaa ggg gaa aat gtc atc acc gcg aaa tcg tca3203Ile Pro Leu Asn Lys Gly Glu Asn Val Ile Thr Ala Lys Ser Ser105510601065tcg gct tcc gga acg act gat gct tca gaa ccg gtc cgg atc gtc3248Ser Ala Ser Gly Thr Thr Asp Ala Ser Glu Pro Val Arg Ile Val107010751080ctt gac cag aaa aag ccg aag ctt aca atc gac acg ccg gaa agc3293Leu Asp Gln Lys Lys Pro Lys Leu Thr Ile Asp Thr Pro Glu Ser108510901095ggc tca aaa ctc aat aaa gag acc gtc acc gtg aaa gga aca gta3338Gly Ser Lys Leu Asn Lys Glu Thr Val Thr Val Lys Gly Thr Val110011051110tca gac gat cac tta gaa tct gtt cat gta aac ggc aaa aaa gca3383Ser Asp Asp His Leu Glu Ser Val His Val Asn Gly Lys Lys Ala111511201125gct gtt gac aat ggc gaa tat tct gca agg att atg ctt gac aac3428Ala Val Asp Asn Gly Glu Tyr Ser Ala Arg Ile Met Leu Asp Asn113011351140ggc aaa aat gaa atc aaa gta acg gca tcg gat gcg gca ggc aac3473Gly Lys Asn Glu Ile Lys Val Thr Ala Ser Asp Ala Ala Gly Asn114511501155aaa acg acc aaa aag gtc acg gta gat gtc aac ttt gaa gcg ccg3518Lys Thr Thr Lys Lys Val Thr Val Asp Val Asn Phe Glu Ala Pro116011651170caa atc acc ggc ttg aaa ccg gcc gaa gat ctc gag ctc aaa acg3563Gln Ile Thr Gly Leu Lys Pro Ala Glu Asp Leu Glu Leu Lys Thr117511801185gga gaa acc gtg aaa atc gag ttt gaa agc gcc gct gat ttg gat3608Gly Glu Thr Val Lys Ile Glu Phe Glu Ser Ala Ala Asp Leu Asp119011951200gct gtc ttt gtg atc aga atg ccg ctg acc aat ttc aaa acc gct3653Ala Val Phe Val Ile Arg Met Pro Leu Thr Asn Phe Lys Thr Ala120512101215gcc caa aac gta acg gag ctg ccg atc aga gaa gtc tca aaa gga3698Ala Gln Asn Val Thr Glu Leu Pro Ile Arg Glu Val Ser Lys Gly122012251230aaa tat gaa gga tat tgg acc gct act tca act gca aaa gca aaa3743Lys Tyr Glu Gly Tyr Trp Thr Ala Thr Ser Thr Ala Lys Ala Lys123512401245gga gcg gaa atc gag gtc atc gtc aga gat gat tac ggc aat gaa3788Gly Ala Glu Ile Glu Val Ile Val Arg Asp Asp Tyr Gly Asn Glu
125012551260acg aga caa acg gca aaa ggc aag ctg tat atc aat gaa aag ctg 3833Thr Arg Gln Thr Ala Lys Gly Lys Leu Tyr Ile Asn Glu Lys Leu126512701275aaa taaaggtgaa aagacgctgt ctttaatggc agcgtttttt tcgttttacg3886Lysatcgacaaat tcagtacgaa aacttcaaaa aatgtacgat ttacgcaaca ttaattgaca 3946gactttacct ttgggcttga tttatactta ggaaaacaaa cactaaggtc accgagccgc 4006agaaagggga aggatgtgga aatctattta gatgcgatat ggctgttaaa cttttgtttt 4066gacttgctgc ttttaatgat gaccgcattt attttaaagc gaagggttaa aaagcggagg 4126ctgatcctag gggcatttgt cgcgtcaagc atcgttctgt ttatgtttac acctttttca 4186ccgtacgtcc ttcatcctgc cggcaaactg tcgttttcgg ttgtgatcgt tcttgtggca 4246tttggtttta agcggttccg gttttttttg cagaatttgt tttcttttta ttttgccact 4306tttttaatgg gaggaggga 4325<210>2<211>1278<212>PRT<213>地衣芽孢桿菌<400>2Glu Gln Val Ala Ala Phe Pro Glu Val Glu Lys Val Leu Pro Asn Glu1 5 10 15Lys Arg Gln Leu Ile Lys Pro Thr Lys Gln Ser Val Lys Lys Ser Ala20 25 30Ala Lys Asp Glu Lys Glu Ile Glu Trp Asn Ile Asn Arg Val Asp Ala35 40 45Pro Lys Ala Trp Lys Leu Gly Tyr Asp Gly Ser Gly Thr Val Val Ala50 55 60Ser Ile Asp Thr Gly Val Gln Trp Asp His Pro Ala Leu Lys Glu Lys65 70 75 80Tyr Arg Gly Tyr Asp Pro Gln His Pro Asp Gln Pro Asn His Glu Phe85 90 95Ser Trp Tyr Asp Ala Val Ser Gly Ala Ser Glu Pro Phe Asp Asp Leu100 105 110Glu His Gly Thr His Val Thr Gly Thr Met Val Gly Ser Glu Pro Asp115 120 125Gly Gln Asn Gln Ile Gly Val Ala Pro Gly Ala Lys Trp Ile Ala Val130 135 140
Lys Ala Phe Ser Asp Asp Gly Gly Thr Asp Glu Asp Leu Ile Ala Ala145 150 155 160Gly Glu Trp Ile Leu Ala Pro Lys Asp Lys Asp Gly Asn Pro His Pro165 170 l75Glu Met Ala Pro Asp Val Val Asn Asn Ser Trp Ser Gly Gly Ala Gly180 185 190Ile Asp Glu Phe Tyr Arg Asp Ile Val Lys Ala Trp Arg Ala Ala Gly195 200 205Ile Phe Pro Glu Phe Ser Ala Gly Asn Val Asp Phe Ala Asn Pro Gly210 215 220Gly Pro Ala Ser Ile Ala Asn Pro Ala Asn Tyr Pro Glu Ala Phe Ala225 230 235 240Thr Gly Ala Thr Asp Ile Asp Asn Arg Leu Ala Asp Phe Ser Leu Gln245 250 255Gly Pro Ser Pro Tyr Asp Glu Thr Lys Pro Glu Ile Ser Ala Pro Gly260 265 270Val Asn Ile Arg Ser Ser Val Pro Gly Ser Gly Tyr Gln Asp Gly Trp275 280 285Asp Gly Thr Ser Met Ala Gly Pro His Val Ala Ala Ala Ala Ala Leu290 295 300Ile Lys Gln Ala Asp Ser Ser Ile Thr Val Asp Glu Thr Glu Lys Ile305 310 315 320Leu Met Glu Thr Ala Thr Pro Leu Thr Asp Ser Lys Phe Thr Glu Ser325 330 335Pro Asn Asn Gly Tyr Gly His Gly Leu Val Asn Val Phe Asp Ala Val340 345 350Ser Ala Val Thr Asp Gly Leu Gly Arg Ala Glu Gly Gln Val Gly Lys355 360 365Glu Gly Glu Asp Lys Ser Pro Pro Ala Leu Asn His Gln Glu Ile Thr370 375 380Glu Ile Tyr Ser Gly Ser Glu Thr Pro Leu Lys Ala Asp Val Glu Asp385 390 395 400Asp Val Ser Ile Ile Ser Val Lys Leu Ser Tyr Lys Thr Asp Asp Ala405 410 415
Asp Trp Glu Thr Ile Ala Ala Lys Gln Thr Ser Gly Asp Tyr Lys Lys420 425 430Gly Thr Tyr Glu Ala Ala Val Thr Val Ser Glu Gly Lys Thr Leu Ser435 440 445Tyr Lys Trp Ile Val Thr Asp Phe Gly Gly Asn Lys Thr Glu Ser Lys450 455 460Val Tyr Glu Val Pro Ile Ser Pro Ala Val Thr Thr Gly Tyr Lys Gln465 470 475 480Asp Phe Glu Asn Ser Ala Ser Gly Trp Leu Thr Ser Gly Val Lys Asn485 490 495Ser Trp Glu Arg Gly Ile Pro Gln Ser Gly Pro Asn Ala Ala Ala Ser500 505 510Gly Lys Asn Val Phe Ala Thr Asn Leu Thr Gly Pro Tyr Glu Ser Ser515 520 525Ala Asn Met Asn Leu Leu Met Pro Pro Val Ser Val Pro Lys Asn Gln530 535 540Lys Leu Tyr Leu Thr Tyr Lys Tyr Trp Arg Asp Ile Glu Glu Asp Phe545 550 555 560Asp Tyr Gly Phe Val Tyr Val Gln Pro Glu Gly Lys Gly Glu Trp Ile565 570 575Pro Ala Ala Glu Tyr Ser Gly Lys Thr Ser Glu Trp Lys Asp Gly Gln580 585 590Ile Asp Leu Ser Glu Tyr Gly Gly Gln Thr Ile Lys Val Met Phe Asn595 600 605Leu Gln Ser Asp Asp Ser Ile Glu Gly Asp Gly Leu Tyr Ile Asp Asp610 615 620Val Ala Leu Val Lys Glu Val Lys Ser Ala Gly Thr Lys Lys Arg Leu625 630 635 640Gly Val Glu Lys G1n Pro Ala Lys Met Lys Asp Lys Lys Thr Lys Lys645 650 655Arg Met Ile Asp Pro Lys Lys Ala Lys Pro Ala Glu Ala Leu Gln Glu660 665 670Lys Thr Glu Thr Lys Lys Ala Ala Pro Ala Val Leu Pro Val Arg A1a675 680 685Gln Val Ser Val Leu Glu Thr Gly Lys Ser Thr Tyr Ser Asn Gln Ala
690 695 700Thr Gly Ala Tyr Ser Leu Ala His Ala Pro Gly Thr Tyr Thr Leu Lys705 710 715 720Ala Glu Ala Tyr Gly Tyr Glu Ser Thr Ala Gln Thr Val Lys Ile Glu725 730 735Ser Asp Lys Thr Thr Thr Ala Asp Phe Val Leu Lys Glu Leu Lys Lys740 745 750Gly Thr Leu Thr Gly Thr Ile Lys Asn Lys Lys Thr Gly Glu Pro Val755 760 765Arg His Ala Lys Leu Tyr Ile Val Glu Asp Ala Ala Val Lys Pro Val770 775 780Gln Thr Asp Asp Asp Gly Ser Tyr Ser Leu Thr Ala Tyr Glu Gly Ser785 790 795 800Tyr Thr Val Lys Val Ser Ala Asn Gly Tyr Tyr Ser Ser Glu Phe Ser805 810 815Val Asp Leu Lys Gly Asp Val Ser Lys Asp Ile Asp Leu Asp Pro Phe820 825 830Ile Gly Tyr Pro Gly Glu Ile Gly Tyr Asp Asp Gly Thr Gly Glu Asn835 840 845Ala Trp Ala Phe Tyr Glu Ser Gly Asn Gly Leu Ala Val Lys Met Thr850 855 860Leu Glu Asn Gly Gln Glu Lys Ala Met Leu Lys Gly Gly Leu Phe Lys865 870 875 880Phe Trp Asp Thr Glu Phe Pro Asp Pro Gly Gly Thr Asp Phe Ala Val885 890 895Glu Val Tyr Asp Ala Ser Gly Glu Lys Gly Ser Pro Gly Lys Lys Ile900 905 910Ala Gly Pro Phe Lys Ala Glu Ala Leu Arg Thr Gly Glu Trp Thr Thr915 920 925Val Asp Leu Gly Asp Glu Gly Ile Ile Val Gly Lys Asp Phe Tyr Leu930 935 940Val Tyr Val Gln Lys Glu Asp Leu Ala Asn Ser Pro Gly Leu Ala Thr945 950 955 960Asp Glu Asp Gly Glu Tyr Ser Gly Arg Asn Trp Gln Tyr Thr Asp Gly965 970 975
Ser Trp Ser Lys Ala Pro Ser Asp Gln Gly Asn Phe Met Ile Arg Ala980 985 990Leu Val Asp Tyr Glu Leu Ser Val Pro Val Ile Thr Ser Pro Lys Asp995 10001005Gly Phe Ile Thr Asn Gln Lys Asn Ala Val Ile Glu Gly Thr Ser101010151020Ser Pro Asn Thr Thr Val His Leu Phe Asn Gly Asp Glu Glu Ala102510301035Gly Thr Ala Glu Thr Ala Ala Asp Gly Thr Phe Ser Lys Glu Ile104010451050Pro Leu Asn Lys Gly Glu Asn Val Ile Thr Ala Lys Ser Ser Ser105510601065Ala Ser Gly Thr Thr Asp Ala Ser Glu Pro Val Arg Ile Val Leu107010751080Asp Gln Lys Lys Pro Lys Leu Thr Ile Asp Thr Pro Glu Ser Gly108510901095Ser Lys Leu Asn Lys Glu Thr Val Thr Val Lys Gly Thr Val Ser110011051110Asp Asp His Leu Glu Ser Val His Val Asn Gly Lys Lys Ala Ala111511201125Val Asp Asn Gly Glu Tyr Ser Ala Arg Ile Met Leu Asp Asn Gly113011351140Lys Asn Glu Ile Lys Val Thr Ala Ser Asp Ala Ala Gly Asn Lys114511501155Thr Thr Lys Lys Val Thr Val Asp Val Asn Phe Glu Ala Pro Gln116011651170Ile Thr Gly Leu Lys Pro Ala Glu Asp Leu Glu Leu Lys Thr Gly117511801185Glu Thr Val Lys Ile Glu Phe Glu Ser Ala Ala Asp Leu Asp Ala119011951200Val Phe Val Ile Arg Met Pro Leu Thr Asn Phe Lys Thr Ala Ala120512101215Gln Asn Val Thr Glu Leu Pro Ile Arg Glu Val Ser Lys Gly Lys122012251230
Tyr Glu Gly Tyr Trp Thr Ala Thr Ser Thr Ala Lys Ala Lys Gly123512401245Ala Glu Ile Glu Val Ile Val Arg Asp Asp Tyr Gly Asn Glu Thr125012551260Arg Gln Thr Ala Lys Gly Lys Leu Tyr Ile Asn Glu Lys Leu Lys126512701275<210>3<211>3405<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(2906)<400>3aatgatatca aacctttcat accgatccct ccagtttcgt tttgataaaa ctagcaactc60tattaaactt tcttgctcta tcttatccca gcaaaatgaa aatgtttgtc acaatgtgtg 120tgcaaaatga ttctagtttt tagaagtttt gttgaaaact gaaggaatcg catgattcag 180cggatacaaa ccatgaatgt aacttactca cagcttatcc taaggataaa cacatattac 240ccacaggata tatccacata tccacatact tattcaatat ttagtataag aacgtatatt 300ccctacaata tctatacaca agtttattca cttatacaca gtaaattgtg cataaatcta 360gagaaattca ctccaataca ttgaatcttt gaaaattatt tctatatata gaaggatttt 420tttgaaactg agagaatatt ttaaaagttc gaaactctaa taattacaaa gaaacattta 480tccagaaggg ggaaaacaga ttg aga aaa agt atc gtg cgc tat ttt gtt atg 533Leu Arg Lys Ser Ile Val Arg Tyr Phe Val Met1 5 10gct ttt att cta tta ttt gcg tta tcc aca ttc ctc acc gga gtg cag 581Ala Phe Ile Leu Leu Phe Ala Leu Ser Thr Phe Leu Thr Gly Val Gln15 20 25gca act tcc gtt ccc gat aaa aag tcg cct gag ctt gag aaa gct gaa 629Ala Thr Ser Val Pro Asp Lys Lys Ser Pro Glu Leu Glu Lys Ala Glu30 35 40atc tac ggt gat att gat gtg acg tct gat aaa cag acg acg gtt atc 677Ile Tyr Gly Asp Ile Asp Val Thr Ser Asp Lys Gln Thr Thr Val Ile45 50 55gtg gaa ctg aaa gaa aag tcg ctt gcc gaa gca aaa gcg gac gga gaa 725Val Glu Leu Lys Glu Lys Ser Leu Ala Glu Ala Lys Ala Asp Gly Glu60 65 70 75aaa caa acg aag gct tct cta aaa aca gct cga agc aaa gcg ctg aaa 773Lys Gln Thr Lys Ala Ser Leu Lys Thr Ala Arg Ser Lys Ala Leu Lys80 85 90aca ctt aaa aaa gcg aaa gta aac cgc gaa tac gac cgt gta ttt tcg 821Thr Leu Lys Lys Ala Lys Val Asn Arg Glu Tyr Asp Arg Val Phe Ser95 100 105ggc ttt tct atg aaa ctg ccg gcc agt gaa att cca aag ctg ctc gcc 869
Gly Phe Ser Met Lys Leu Pro Ala Ser Glu Ile Pro Lys Leu Leu Ala110 115 120gtc aag gaa gtt aaa gcg gtt tat cca aac gca act tac aaa cct gac 917Val Lys Glu Val Lys Ala Val Tyr Pro Asn Ala Thr Tyr Lys Pro Asp125 130 135agc gta aaa gga aaa gac gtg aca ctt gca gca gac gcc att tat cca 965Ser Val Lys Gly Lys Asp Val Thr Leu Ala Ala Asp Ala Ile Tyr Pro140 145 150 155cag atg gat aaa agt gcc ccg ttc atc gga gcg gat cag gca tgg aaa1013Gln Met Asp Lys Ser Ala Pro Phe Ile Gly Ala Asp Gln Ala Trp Lys160 165 170tcg ggc tat acc ggt aaa ggc att aag gta gcc gtt att gat acg ggc1061Ser Gly Tyr Thr Gly Lys Gly Ile Lys Val Ala Val Ile Asp Thr Gly175 180 185gtg gat tac acc cat ccc gac tta aag aat aat ttc ggc cca tat aag1109Val Asp Tyr Thr His Pro Asp Leu Lys Asn Asn Phe Gly Pro Tyr Lys190 195 200ggc tat gac ttt gtg gat aat gat tat gat cca cag gaa aca ccg aca1157Gly Tyr Asp Phe Val Asp Asn Asp Tyr Asp Pro Gln Glu Thr Pro Thr205 210 215ggc gac ccg cgc ggc gga gcg act gat cac ggc act cat gtt gca gga1205Gly Asp Pro Arg Gly Gly Ala Thr Asp His Gly Thr His Val Ala Gly220 225 230 235aca atc gcc gcg aac gga cag att aaa ggc gtg gcg cct gaa gca acg1253Thr Ile Ala Ala Asn Gly Gln Ile Lys Gly Val Ala Pro Glu Ala Thr240 245 250ctt ctt gct tac cgc gtg ctc ggc ccc ggc ggc tca ggc aca acc gag1301Leu Leu Ala Tyr Arg Val Leu Gly Pro Gly Gly Ser Gly Thr Thr Glu255 260 265aat gtg att gcg ggc att gaa aaa gcc gtt gca gac gga gca aaa gtg1349Asn Val Ile Ala Gly Ile Glu Lys Ala Val Ala Asp Gly Ala Lys Val270 275 280atg aac ctc tca ttg gga aat tcg ctg aac agc cct gat tat gca aca1397Met Asn Leu Ser Leu Gly Asn Ser Leu Asn Ser Pro Asp Tyr Ala Thr285 290 295agc atc gca ctg gac tgg gcg atg gct gaa ggg gtt gtc gcc gtt acg1445Ser Ile Ala Leu Asp Trp Ala Met Ala Glu Gly Val Val Ala Val Thr300 305 310 315tca aac ggt aac agc gga ccg gaa aac tgg acg gtc ggc tcg ccg gga1493Ser Asn Gly Asn Ser Gly Pro Glu Asn Trp Thr Val Gly Ser Pro Gly320 325 330aca tca agg gtt gcg att tcc gtc ggc gca tca cag ctt ccg tat aat1541Thr Ser Arg Val Ala Ile Ser Val Gly Ala Ser Gln Leu Pro Tyr Asn335 340 345gag tat tcg gtg acg ctt cct tcg tat tca tca gca aag gtg atg gga1589Glu Tyr Ser Val Thr Leu Pro Ser Tyr Ser Ser Ala Lys Val Met Gly350 355 360tac caa gag gaa aag gat ctt gaa gca ttg aac ggt caa gag gtg gaa1637Tyr Gln Glu Glu Lys Asp Leu Glu Ala Leu Asn Gly Gln Glu Val Glu365 370 375ctc gtt gaa gcc ggc ctc gga caa gct gac gat ttc tca ggc aaa gat1685Leu Val Glu Ala Gly Leu Gly Gln Ala Asp Asp Phe Ser Gly Lys Asp
380 385 390 395gtg aaa gga aaa gtt gcg gtt atc cag aga ggc gtc ata cca ttt gtt1733Val Lys Gly Lys Val Ala Val Ile Gln Arg Gly Val Ile Pro Phe Val400 405 410gat aag gct gaa aat gcc aaa aac gcc ggc gct atc gga gcc gtg att178lAsp Lys Ala Glu Asn Ala Lys Asn Ala Gly Ala Ile Gly Ala Val Ile415 420 425tac aat aat gca aca ggg gaa atc gag gca aac gtc atg ggg atg gcc1829Tyr Asn Asn Ala Thr Gly Glu Ile Glu Ala Asn Val Met Gly Met Ala430 435 440gta ccg acc gtc aaa ttg tca aaa gaa gag ggc gaa aag ctt gtt caa1877Val Pro Thr Val Lys Leu Ser Lys Glu Glu Gly Glu Lys Leu Val Gln445 450 455cag atc aaa gaa ggg aaa cac tcc gtt gtc ttt tct ttc aaa ttg gac1925Gln Ile Lys Glu Gly Lys His Ser Val Val Phe Ser Phe Lys Leu Asp460 465 470 475aaa aag ctg ggg gaa aca att gcc tca ttc tcg tcc cgc ggg cct gtc1973Lys Lys Leu Gly Glu Thr Ile Ala Ser Phe Ser Ser Arg Gly Pro Val480 485 490atg gat aca tgg atg att aaa cct gac gtt tca gcg cca ggc gtc aac2021Met Asp Thr Trp Met Ile Lys Pro Asp Val Ser Ala Pro Gly Val Asn495 500 505atc gtc agc acc att ccg acc cac gat ccg aaa aac ccg tac ggt tac2069Ile Val Ser Thr Ile Pro Thr His Asp Pro Lys Asn Pro Tyr Gly Tyr510 515 520ggt tca aaa cag gga aca agc atg gct tcc ccg cat gtc gcg gga acg2117Gly Ser Lys Gln Gly Thr Ser Met Ala Ser Pro His Val Ala Gly Thr525 530 535gca gcc ata tta aag cag gcc aaa ccg gat tgg acg cct gag caa atc2165Ala Ala Ile Leu Lys Gln Ala Lys Pro Asp Trp Thr Pro Glu Gln Ile540 545 550 555aaa ggc gta ctg atg aat acg gcg gaa aaa ctg acg gat gaa aat gga2213Lys Gly Val Leu Met Asn Thr Ala Glu Lys Leu Thr Asp Glu Asn Gly560 565 570aag cct ctc cct cac aat acg caa gga gcg ggc agc atc cgc atc atg2261Lys Pro Leu Pro His Asn Thr Gln Gly Ala Gly Ser Ile Arg Ile Met575 580 585gag gcc ctt aaa gct tca tcc att gta acg ccg ggc agc cat tca tac2309Glu Ala Leu Lys Ala Ser Ser Ile Val Thr Pro Gly Ser His Ser Tyr590 595 600gga aca ttc ctg aaa gac aaa ggg aaa cag acg aaa aaa caa gcg ttc2357Gly Thr Phe Leu Lys Asp Lys Gly Lys Gln Thr Lys Lys Gln Ala Phe605 610 615acg att gaa aac ctt tct tca cac aga aaa gcc tat cag ctc gaa tac2405Thr Ile Glu Asn Leu Ser Ser His Arg Lys Ala Tyr Gln Leu Glu Tyr620 625 630 635tcc ttt aaa gga acg ggc atc acg gta tca gga acg gaa cga gtc gtg2453Ser Phe Lys Gly Thr Gly Ile Thr Val Ser Gly Thr Glu Arg Val Val640 645 650gta ccg gcc aat caa aca ggt aaa gca gcg gca aaa gta acc gtc aat2501Val Pro Ala Asn Gln Thr Gly Lys Ala Ala Ala Lys Val Thr Val Asn655 660 665
tcc gcg aaa acg aaa gca ggc aca tat gaa ggc acg gtt tac atc cgt2549Ser Ala Lys Thr Lys Ala Gly Thr Tyr Glu Gly Thr Val Tyr Ile Arg670 675 680gaa gac gga aga aaa gtc gcc gaa atc ccg ctc cta ttg atc gtc aaa2597Glu Asp Gly Arg Lys Val Ala Glu Ile Pro Leu Leu Leu Ile Val Lys685 690 695gag cca gac tac ccg cgc gtc aca tcc gta aca gtt gaa ccg gga gca2645Glu Pro Asp Tyr Pro Arg Val Thr Ser Val Thr Val Glu Pro Gly Ala700 705 710 715aag cag ggc gct tac acg atc gaa gcc tac ctg ccg ggc ggg gct gaa2693Lys Gln Gly Ala Tyr Thr Ile Glu Ala Tyr Leu Pro Gly Gly Ala Glu720 725 730gag ctc gca ttt ctc gtc tat gat gaa aac ctg aac ctt ctc ggc cag2741Glu Leu Ala Phe Leu Val Tyr Asp Glu Asn Leu Asn Leu Leu Gly Gln735 740 745gcc ggc gtt tac aaa aac cag ggc aaa ggc tat caa tct tat caa tgg2789Ala Gly Val Tyr Lys Asn Gln Gly Lys Gly Tyr Gln Ser Tyr Gln Trp750 755 760aac ggc aaa atc aat gac gcc gca tcc ctt aag tcc gga aaa tac tat2837Asn Gly Lys Ile Asn Asp Ala Ala Ser Leu Lys Ser Gly Lys Tyr Tyr765 770 775atg ctt gcc tat gca tcc gcc aaa ggg aaa tca agc tac gta ttg acg2885Met Leu Ala Tyr Ala Ser Ala Lys Gly Lys Ser Ser Tyr Val Leu Thr780 785 790 795gaa gac cct ttt atc gtc gaa taatgacaag ccttgttgag aaccactcaa 2936Glu Asp Pro Phe Ile Val Glu800caaggctttt ttatgttaaa atacggataa tgcgttcagg agaagctccc ccttctcttc 2996aaaacgtgaa aaaagcaatc ggaggacatc gtgtatatgc tttcttttat cgtattattc 3056ggcttatcct tcattattgt ctgctttata tttttcacga ttttgtactt cgccgtcaac 3116ctgcagaagc gcgagcccaa gccttttcaa aaagctgcgg agcaaaccgt cgataccatc 3176atcctcattc cgatcagctg gctgtttacc gctttataca tatgcattct gtttattctt 3236ttcccaatcc gccattttct cgattttttt cagcaaaaac gctaaattga ctgatgaaac 3296gcttcggcca gcagccggta tgaatccaat ctgtcttgaa aatcgtgggt gatcgtcacc 3356gccatgattt cgtccgttcc gtaagcgccg gccagttcaa gcagctgtt 3405<210>4<211>802<212>PRT<213>地衣芽孢桿菌<400>4Leu Arg Lys Ser Ile Val Arg Tyr Phe Val Met Ala Phe Ile Leu Leu1 5 10 15Phe Ala Leu Ser Thr Phe Leu Thr Gly Val Gln Ala Thr Ser Val Pro20 25 30
Asp Lys Lys Ser Pro Glu Leu Glu Lys Ala Glu Ile Tyr Gly Asp Ile35 40 45Asp Val Thr Ser Asp Lys Gln Thr Thr Val Ile Val Glu Leu Lys Glu50 55 60Lys Ser Leu Ala Glu Ala Lys Ala Asp Gly Glu Lys Gln Thr Lys Ala65 70 75 80Ser Leu Lys Thr Ala Arg Ser Lys Ala Leu Lys Thr Leu Lys Lys Ala85 90 95Lys Val Asn Arg Glu Tyr Asp Arg Val Phe Ser Gly Phe Ser Met Lys100 105 110Leu Pro Ala Ser Glu Ile Pro Lys Leu Leu Ala Val Lys Glu Val Lys115 120 125Ala Val Tyr Pro Asn Ala Thr Tyr Lys Pro Asp Ser Val Lys Gly Lys130 135 140Asp Val Thr Leu Ala Ala Asp Ala Ile Tyr Pro Gln Met Asp Lys Ser145 150 155 160Ala Pro Phe Ile Gly Ala Asp Gln Ala Trp Lys Ser Gly Tyr Thr Gly165 170 175Lys Gly Ile Lys Val Ala Val Ile Asp Thr Gly Val Asp Tyr Thr His180 185 190Pro Asp Leu Lys Asn Asn Phe Gly Pro Tyr Lys Gly Tyr Asp Phe Val195 200 205Asp Asn Asp Tyr Asp Pro Gln Glu Thr Pro Thr Gly Asp Pro Arg Gly210 215 220Gly Ala Thr Asp His Gly Thr His Val Ala Gly Thr Ile Ala Ala Asn225 230 235 240Gly Gln Ile Lys Gly Val Ala Pro Glu Ala Thr Leu Leu Ala Tyr Arg245 250 255Val Leu Gly Pro Gly Gly Ser Gly Thr Thr Glu Asn Val Ile Ala Gly260 265 270Ile Glu Lys Ala Val Ala Asp Gly Ala Lys Val Met Asn Leu Ser Leu275 280 285Gly Asn Ser Leu Asn Ser Pro Asp Tyr Ala Thr Ser Ile Ala Leu Asp290 295 300Trp Ala Met Ala Glu Gly Val Val Ala Val Thr Ser Asn Gly Asn Ser
305 310 315 320Gly Pro Glu Asn Trp Thr Val Gly Ser Pro Gly Thr Ser Arg Val Ala325 330 335Il e Ser Val Gly Ala Ser Gln Leu Pro Tyr Asn Glu Tyr Ser Val Thr340 345 350Leu Pro Ser Tyr Ser Ser Ala Lys Val Met Gly Tyr Gln Glu Glu Lys355 360 365Asp Leu Glu Ala Leu Asn Gly Gln Glu Val Glu Leu Val Glu Ala Gly370 375 380Leu Gly Gln Ala Asp Asp Phe Ser Gly Lys Asp Val Lys Gly Lys Val385 390 395 400Ala Val Ile Gln Arg Gly Val Ile Pro Phe Val Asp Lys Ala Glu Asn405 410 415Ala Lys Asn Ala Gly Ala Ile Gly Ala Val Ile Tyr Asn Asn Ala Thr420 425 430Gly Glu Ile Glu Ala Asn Val Met Gly Met Ala Val Pro Thr Val Lys435 440 445Leu Ser Lys Glu Glu Gly Glu Lys Leu Val Gln Gln Ile Lys Glu Gly450 455 460Lys His Ser Val Val Phe Ser Phe Lys Leu Asp Lys Lys Leu Gly Glu465 470 475 480Thr Ile Ala Ser Phe Ser Ser Arg Gly Pro Val Met Asp Thr Trp Met485 490 495Ile Lys Pro Asp Val Ser Ala Pro Gly Val Asn Ile Val Ser Thr Ile500 505 510Pro Thr His Asp Pro Lys Asn Pro Tyr Gly Tyr Gly Ser Lys Gln Gly515 520 525Thr Ser Met Ala Ser Pro His Val Ala Gly Thr Ala Ala Ile Leu Lys530 535 540Gln Ala Lys Pro Asp Trp Thr Pro Glu Gln Ile Lys Gly Val Leu Met545 550 555 560Asn Thr Ala Glu Lys Leu Thr Asp Glu Asn Gly Lys Pro Leu Pro His565 570 575Asn Thr Gln Gly Ala Gly Ser Ile Arg Ile Met Glu Ala Leu Lys Ala580 585 590
Ser Ser Ile Val Thr Pro Gly Ser His Ser Tyr Gly Thr Phe Leu Lys595 600 605Asp Lys Gly Lys Gln Thr Lys Lys Gln Ala Phe Thr Ile Glu Asn Leu610 615 620Ser Ser His Arg Lys Ala Tyr Gln Leu Glu Tyr Ser Phe Lys Gly Thr625 630 635 640Gly Ile Thr Val Ser Gly Thr Glu Arg Val Val Val Pro Ala Asn Gln645 650 655Thr Gly Lys Ala Ala Ala Lys Val Thr Val Asn Ser Ala Lys Thr Lys660 665 670Ala Gly Thr Tyr Glu Gly Thr Val Tyr Ile Arg Glu Asp Gly Arg Lys675 680 685Val Ala Glu Ile Pro Leu Leu Leu Ile Val Lys Glu Pro Asp Tyr Pro690 695 700Arg Val Thr Ser Val Thr Val Glu Pro Gly Ala Lys Gln Gly Ala Tyr705 710 715 720Thr Ile Glu Ala Tyr Leu Pro Gly Gly Ala Glu Glu Leu Ala Phe Leu725 730 735Val Tyr Asp Glu Asn Leu Asn Leu Leu Gly Gln Ala Gly Val Tyr Lys740 745 750Asn Gln Gly Lys Gly Tyr Gln Ser Tyr Gln Trp Asn Gly Lys Ile Asn755 760 765Asp Ala Ala Ser Leu Lys Ser Gly Lys Tyr Tyr Met Leu Ala Tyr Ala770 775 780Ser Ala Lys Gly Lys Ser Ser Tyr Val Leu Thr Glu Asp Pro Phe Ile785 790 795 800Val Glu<210>5<211>2771<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(175)..(2268)<400>5
gggcctactg tccttaaaaa attacacttc tatccagaac aaaagaaaaa gctagaactt 60gaaagtcaaa gaaggtcaat gtatactaat agteagaaat ggtcaaactg cagaccattt 120tttctaagtc aattggtcaa aaaaggtcaa attcaataaa gggggttttg gcaa atg177Met1cgt tgt caa cat tgt caa aaa aat gag gcg acg att cgc cta aac atg225Arg Cys Gln His Cys Gln Lys Asn Glu Ala Thr Ile Arg Leu Asn Met5 10 15caa atc aac ttt aca aaa aaa caa atg gat tta tgt gaa gac tgc tat273Gln Ile Asn Phe Thr Lys Lys Gln Met Asp Leu Cys Glu Asp Cys Tyr20 25 30aaa gag ctg act cag cct tct atg ttt tca gga aat cac ttt ttt ggc321Lys Glu Leu Thr Gln Pro Ser Met Phe Ser Gly Asn His Phe Phe Gly35 40 45ggc gga ggt tct ttc ttc agc cag cca tca caa gcg cag acg caa acc369Gly Gly Gly Ser Phe Phe Ser Gln Pro Ser Gln Ala Gln Thr Gln Thr50 55 60 65gct gcc aaa aaa ggc ttg ctc gat gaa ctt gcc gtc aat tta aca aat417Ala Ala Lys Lys Gly Leu Leu Asp Glu Leu Ala Val Asn Leu Thr Asn70 75 80gca gca aag acc ggc ttg atc gat ccg gtg atc ggc cgc gat gat gaa465Ala Ala Lys Thr Gly Leu Ile Asp Pro Val Ile Gly Arg Asp Asp Glu85 90 95gta gcc cgt gtg atc gag atc tta aac cgc aga aat aaa aac aat ccg513Val Ala Arg Val Ile Glu Ile Leu Asn Arg Arg Asn Lys Asn Asn Pro100 105 110gtc tta atc ggt gaa ccg ggc gtc ggt aaa aca gcc gtc gct gaa gga561Val Leu Ile Gly Glu Pro Gly Val Gly Lys Thr Ala Val Ala Glu Gly115 120 125ctt gct ctc aaa atc gcc gaa ggc gat gtt cca aat aaa ttg aaa aac609Leu Ala Leu Lys Ile Ala Glu Gly Asp Val Pro Asn Lys Leu Lys Asn130 135 140 145aaa gag ctc tac ctg ctt gat gtc gct tcc ctc gtg gca aac acc ggc657Lys Glu Leu Tyr Leu Leu Asp Val Ala Ser Leu Val Ala Asn Thr Gly150 155 160atc aga ggg caa ttt gaa gaa aga atg aaa cag ctg atg aaa gag ctg705Ile Arg Gly Gln Phe Glu Glu Arg Met Lys Gln Leu Met Lys Glu Leu165 170 175aaa gaa cgc aaa aac gca atc ttg ttt att gac gaa att cac ctt ctt753Lys Glu Arg Lys Asn Ala Ile Leu Phe Ile Asp Glu Ile His Leu Leu180 185 190gtc ggg gcc ggc tct gca gaa ggc tcc atg gat gcg ggc aac att tta801Val Gly Ala Gly Ser Ala Glu Gly Ser Met Asp Ala Gly Asn Ile Leu195 200 205aaa cct gca ctt gcc aga ggc gag ctg caa gtt atc ggc gct acg acg849Lys Pro Ala Leu Ala Arg Gly Glu Leu Gln Val Ile Gly Ala Thr Thr210 215 220 225ttg aag gaa tat cgc caa att gaa aaa gat gcg gcg tta gag cgg cgc897Leu Lys Glu Tyr Arg Gln Ile Glu Lys Asp Ala Ala Leu Glu Arg Arg230 235 240ttt cag ccg atc atg gtg cac gaa ccg aca att gag cag gcc gca gcg945
Phe Gln Pro Ile Met Val His Glu Pro Thr Ile Glu Gln Ala Ala Ala245 250 255att tta aac ggg ctc aag gaa aaa tac gaa gca tat cac gat gtg act 993Ile Leu Asn Gly Leu Lys Glu Lys Tyr Glu Ala Tyr His Asp Val Thr260 265 270tac acg gat gaa gcg atc aaa gcc tgc gtc att ctg tca agc cgc tat1041Tyr Thr Asp Glu Ala Ile Lys Ala Cys Val Ile Leu Ser Ser Arg Tyr275 280 285att cag gac cgc cat ctt ccc gat aag gcg atc gac ctt ttg gat gaa1089Ile Gln Asp Arg His Leu Pro Asp Lys Ala Ile Asp Leu Leu Asp Glu290 295 300 305gcc ggt tca aaa gcg aac ttg aaa atc gac acg gtc agc ggt gaa caa1137Ala Gly Ser Lys Ala Asn Leu Lys Ile Asp Thr Val Ser Gly Glu Gln310 315 320gct tcc caa cgc ctg caa gaa atc gaa gcg gaa aaa gcc aaa gcg ctt1185Ala Ser Gln Arg Leu Gln Glu Ile Glu Ala Glu Lys Ala Lys Ala Leu325 330 335caa gaa gaa aac tac gag ctt gca gca aaa ctc cgc gac gaa gaa gaa1233Gln Glu Glu Asn Tyr Glu Leu Ala Ala Lys Leu Arg Asp Glu Glu Glu340 345 350gcg ctg cat aaa aaa atg gaa agc cat gag gaa gaa aaa cgg gca aca128lAla Leu His Lys Lys Met Glu Ser His Glu Glu Glu Lys Arg Ala Thr355 360 365gtt gaa gcg gaa gac att caa gcg atc atc gaa caa aaa acc ggc att1329Val Glu Ala Glu Asp Ile Gln Ala Ile Ile Glu Gln Lys Thr Gly Ile370 375 380 385ccg gtc ggc aag ctt cag cac gac gat cag aag aaa atg aaa gag ctt1377Pro Val Gly Lys Leu Gln His Asp Asp Gln Lys Lys Met Lys Glu Leu390 395 400gaa gca cac ctt tca agc cgc gtg atc ggt caa aaa gaa gcg gtg aaa1425Glu Ala His Leu Ser Ser Arg Val Ile Gly Gln Lys Glu Ala Val Lys405 410 415aaa gtg gca aaa gcc gtc aga aga agc cgc gcc ggc ctg aaa tca aaa1473Lys Val Ala Lys Ala Val Arg Arg Ser Arg Ala Gly Leu Lys Ser Lys420 425 430aac agg cct gtc ggt tca ttc ctg ttc gtc ggc ccg aca ggt gtc ggt1521Asn Arg Pro Val Gly Ser Phe Leu Phe Val Gly Pro Thr Gly Val Gly435 440 445aaa aca gaa ctt tct aaa agg ctt gcg gaa gaa ctg ttc ggc acg aag1569Lys Thr Glu Leu Ser Lys Arg Leu Ala Glu Glu Leu Phe Gly Thr Lys450 455 460 465gat gcc atc atc cgc ctc gat atg agc gaa tac atg gaa aaa cac gcc1617Asp Ala Ile Ile Arg Leu Asp Met Ser Glu Tyr Met Glu Lys His Ala470 475 480gta tca aag ctg atc ggt tcg cct cct gga tat gtc ggc cac gaa gaa1665Val Ser Lys Leu Ile Gly Ser Pro Pro Gly Tyr Val Gly His Glu Glu485 490 495gcc gga cag ctt acc gaa aaa gtc cgg agg aac ccg tac agc atc gtt1713Ala Gly Gln Leu Thr Glu Lys Val Arg Arg Asn Pro Tyr Ser Ile Val500 505 510ttg ctc gat gaa atc gaa aaa gcc cat ccc gat gtc cag cac atg ttc1761Leu Leu Asp Glu Ile Glu Lys Ala His Pro Asp Val Gln His Met Phe
515 520 525ctg caa atc atg gaa gac gga cgt ctg aca gac agc caa ggg cgg acg1809Leu Gln Ile Met Glu Asp Gly Arg Leu Thr Asp Ser Gln Gly Arg Thr530 535 540 545gtc agc ttt aaa gat acc gtc att att atg aca agc aat gcc ggt gta1857Val Ser Phe Lys Asp Thr Val Ile Ile Met Thr Ser Asn Ala Gly Val550 555 560tct gaa aag cgg aca acc gtc ggt ttt aac cgg gaa gag caa gta atg1905Ser Glu Lys Arg Thr Thr Val Gly Phe Asn Arg Glu Glu Gln Val Met565 570 575ggc gaa caa tca atg atc gat tca tta agc ggc tac ttt aag ccg gaa1953Gly Glu Gln Ser Met Ile Asp Ser Leu Ser Gly Tyr Phe Lys Pro Glu580 585 590ttc ctg aac aga ttt gac agc atc atc gaa ttc cag ccg ctc gaa aaa2001Phe Leu Asn Arg Phe Asp Ser Ile Ile Glu Phe Gln Pro Leu Glu Lys595 600 605gaa gat ctg gtc aag atc gcc gcc ctc ttg ctg aaa gag ctt gaa gaa2049Glu Asp Leu Val Lys Ile Ala Ala Leu Leu Leu Lys Glu Leu Glu Glu610 615 620 625acg tta aaa gag caa aac atg acg ctc gaa gtt tca gat gag gca aaa2097Thr Leu Lys Glu Gln Asn Met Thr Leu Glu Val Ser Asp Glu Ala Lys630 635 640gaa aaa atc gct gaa atc gga tac cat ccg gcc ttt ggc gcc cgc cca2145Glu Lys Ile Ala Glu Ile Gly Tyr His Pro Ala Phe Gly Ala Arg Pro645 650 655tta aga aga acc att caa aca gcg gtc gaa gat caa atg acc gat ctg2193Leu Arg Arg Thr Ile Gln Thr Ala Val Glu Asp Gln Met Thr Asp Leu660 665 670ctg ctt gaa gaa gaa cac atc aaa ggt ttt gcg gtt atc gtt gaa aac2241Leu Leu Glu Glu Glu His Ile Lys Gly Phe Ala Val Ile Val Glu Asn675 680 685gat gaa atc cgg gtg aaa aca acc aga taacacacac aacggcctcc 2288Asp Glu Ile Arg Val Lys Thr Thr Arg690 695gatcgcggag gtcgttttac atacaacata agaatcacaa aagttctatt ttttaacatt 2348aaataaccct aaagtccctt ttcaccatga ttttgcaaca atttaagaaa cagcctaaag 2408ttcctatcac gcaacaccga tattaacgat agaaaagtta gttaaaggaa ttggtgaaaa 2468aatggacaaa acatcgttaa ttggaatcat cttagctatc atcggagtag gtgtcggcat 2528ggtcatgaaa ggtgtcagcc cttccgttct cgtcaatccc gcggcatggc tgatcatcct 2588tgtcgggaca atcgcagcag ttgtgatcgc ttttccaatc cgcgagctca aaaaggttcc 2648caaactcttc ggcatcttgt tcaaagagaa gaagatgatc gaaatcaacg aactgatccc 2708gatgttttcc gagtgggcgc aaatcgcaag acgcgaagga cttctcgctt tggaagcaaa 2768ttt2771<210>6<211>698<212>PRT<213>地衣芽孢桿菌
<400>6Met Arg Cys Gln His Cys Gln Lys Asn Glu Ala Thr Ile Arg Leu Asn1 5 10 15Met Gln Ile Asn Phe Thr Lys Lys Gln Met Asp Leu Cys Glu Asp Cys20 25 30Tyr Lys Glu Leu Thr Gln Pro Ser Met Phe Ser Gly Asn His Phe Phe35 40 45Gly Gly Gly Gly Ser Phe Phe Ser Gln Pro Ser Gln Ala Gln Thr Gln50 55 60Thr Ala Ala Lys Lys Gly Leu Leu Asp Glu Leu Ala Val Asn Leu Thr65 70 75 80Asn Ala Ala Lys Thr Gly Leu Ile Asp Pro Val Ile Gly Arg Asp Asp85 90 95Glu Val Ala Arg Val Ile Glu Ile Leu Asn Arg Arg Asn Lys Asn Asn100 105 110Pro Val Leu Ile Gly Glu Pro Gly Val Gly Lys Thr Ala Val Ala Glu115 120 125Gly Leu Ala Leu Lys Ile Ala Glu Gly Asp Val Pro Asn Lys Leu Lys130 135 140Asn Lys Glu Leu Tyr Leu Leu Asp Val Ala Ser Leu Val Ala Asn Thr145 150 155 160Gly Ile Arg Gly Gln Phe Glu Glu Arg Met Lys Gln Leu Met Lys Glu165 170 175Leu Lys Glu Arg Lys Asn Ala Ile Leu Phe Ile Asp Glu Ile His Leu180 185 190Leu Val Gly Ala Gly Ser Ala Glu Gly Ser Met Asp Ala Gly Asn Ile195 200 205Leu Lys Pro Ala Leu Ala Arg Gly Glu Leu Gln Val Ile Gly Ala Thr2l0 215 220Thr Leu Lys Glu Tyr Arg Gln Ile Glu Lys Asp Ala Ala Leu Glu Arg225 230 235 240Arg Phe Gln Pro Ile Met Val His Glu Pro Thr Ile Glu Gln Ala Ala245 250 255Ala Ile Leu Asn Gly Leu Lys Glu Lys Tyr Glu Ala Tyr His Asp Val260 265 270
Thr Tyr Thr Asp Glu Ala Ile Lys Ala Cys Val Ile Leu Ser Ser Arg275 280 285Tyr Ile Gln Asp Arg His Leu Pro Asp Lys Ala Ile Asp Leu Leu Asp290 295 300Glu Ala Gly Ser Lys Ala Asn Leu Lys Ile Asp Thr Val Ser Gly Glu305 310 315 320Gln Ala Ser Gln Arg Leu Gln Glu Ile Glu Ala Glu Lys Ala Lys Ala325 330 335Leu Gln Glu Glu Asn Tyr Glu Leu Ala Ala Lys Leu Arg Asp Glu Glu340 345 350Glu Ala Leu His Lys Lys Met Glu Ser His Glu Glu Glu Lys Arg Ala355 360 365Thr Val Glu Ala Glu Asp Ile Gln Ala Ile Ile Glu Gln Lys Thr Gly370 375 380Ile Pro Val Gly Lys Leu Gln His Asp Asp Gln Lys Lys Met Lys Glu385 390 395 400Leu Glu Ala His Leu Ser Ser Arg Val Ile Gly Gln Lys Glu Ala Val405 410 415Lys Lys Val Ala Lys Ala Val Arg Arg Ser Arg Ala Gly Leu Lys Ser420 425 430Lys Asn Arg Pro Val Gly Ser Phe Leu Phe Val Gly Pro Thr Gly Val435 440 445Gly Lys Thr Glu Leu Ser Lys Arg Leu Ala Glu Glu Leu Phe Gly Thr450 455 460Lys Asp Ala Ile Ile Arg Leu Asp Met Ser Glu Tyr Met Glu Lys His465 470 475 480Ala Val Ser Lys Leu Ile Gly Ser Pro Pro Gly Tyr Val Gly His Glu485 490 495Glu Ala Gly Gln Leu Thr Glu Lys Val Arg Arg Asn Pro Tyr Ser Ile500 505 510Val Leu Leu Asp Glu Ile Glu Lys Ala His Pro Asp Val Gln His Met515 520 525Phe Leu Gln Ile Met Glu Asp Gly Arg Leu Thr Asp Ser Gln Gly Arg530 535 540
Thr Val Ser Phe Lys Asp Thr Val Ile Ile Met Thr Ser Asn Ala Gly545 550 555 560Val Ser Glu Lys Arg Thr Thr Val Gly Phe Asn Arg Glu Glu Gln Val565 570 575Met Gly Glu Gln Ser Met Ile Asp Ser Leu Ser Gly Tyr Phe Lys Pro580 585 590Glu Phe Leu Asn Arg Phe Asp Ser Ile Ile Glu Phe Gln Pro Leu Glu595 600 605Lys Glu Asp Leu Val Lys Ile Ala Ala Leu Leu Leu Lys Glu Leu Glu610 615 620Glu Thr Leu Lys Glu Gln Asn Met Thr Leu Glu Val Ser Asp Glu Ala625 630 635 640Lys Glu Lys Ile Ala Glu Ile Gly Tyr His Pro Ala Phe Gly Ala Arg645 650 655Pro Leu Arg Arg Thr Ile Gln Thr Ala Val Glu Asp Gln Met Thr Asp660 665 670Leu Leu Leu Glu Glu Glu His Ile Lys Gly Phe Ala Val Ile Val Glu675 680 685Asn Asp Glu Ile Arg Val Lys Thr Thr Arg690 695<210>7<211>2958<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(2459)<400>7gctgacaaaa gatgcgcggg atgttcttcc cgaaagcctc tcttatccga aagccgtgga 60agcaagcgcg gtcagcaata tgctcgtcgc cggactgatg gcgaaagatt ggaagcttgt120cggccgcatg atgcaaaaag acctctttca ccagccatac cgaagggcgc tcgtccctga180gctttcgaaa gtggagcatg aagcaggcca aaacggcgcg ttcgggacgg ctttaagcgg240agccggcccg acgatccttt cctttattga aaaaggaaaa ggagaggcgc tgagaaacca300gcttgcgtcc aagtttccgc actgtgaggt cgactgcctt tatgtcccgg atacaggcat360tatcgttgaa agaaaatcag taaacagtgt ttaacgaaaa aagagctttt tcggctcttt420ttttgtttgc cttcaaaaca aggattttta tttaggaagt cgaagtagta tggcaaacag480
acatgaggag gagaaacgac atg aag cag ctg ata acc gaa aaa gac ctc atc 533Met Lys Gln Leu Ile Thr Glu Lys Asp Leu Ile1 5 10aag ctt gta tcc att acc gat ccc cag tat tcg ccc gat ggt gca aaa581Lys Leu Val Ser Ile Thr Asp Pro Gln Tyr Ser Pro Asp Gly Ala Lys15 20 25atc gcc tac gtc caa aea aaa gta aac gaa aaa cag gac tca tat gat629Ile Ala Tyr Val Gln Thr Lys Val Asn Glu Lys Gln Asp Ser Tyr Asp30 35 40tcg cat atc atg atc tac gac cgc gaa aaa caa gct tcg gtg caa tgg677Ser His Ile Met Ile Tyr Asp Arg Glu Lys Gln Ala Ser Val Gln Trp45 50 55acg ttt ggg aaa ggc aga aac cag cat ccc cgc tgg tcg ccg gac ggc 725Thr Phe Gly Lys Gly Arg Asn Gln His Pro Arg Trp Ser Pro Asp Gly60 65 70 75aaa tac ctt gca ttt acg tcc aat cgc gag gaa acc gca caa att tac 773Lys Tyr Leu Ala Phe Thr Ser Asn Arg Glu Glu Thr Ala Gln Ile Tyr80 85 90gtg ata agc gcc gcc ggg gga gaa gcg aga aaa gtg acc gat att ccc 821Val Ile Ser Ala Ala Gly Gly Glu Ala Arg Lys Val Thr Asp Ile Pro95 100 105tat gat gta tca cag cct gaa tgg tcg ccg gac ggc aag tca ttg ctt 869Tyr Asp Val Ser Gln Pro Glu Trp Ser Pro Asp Gly Lys Ser Leu Leu110 115 120tgt tca gtg aag ctg aca aaa gag gaa agc gtc gac gat gag aag aaa 917Cys Ser Val Lys Leu Thr Lys Glu Glu Ser Val Asp Asp Glu Lys Lys125 130 135acc gag att gaa gac cac gaa ccg ctg gaa gtc gat tct ttg tca tat 965Thr Glu Ile Glu Asp His Glu Pro Leu Glu Val Asp Ser Leu Ser Tyr140 145 150 155aaa gca gac ggc caa ggg ttt aaa agg gga aag tat acg cag ctt gtc1013Lys Ala Asp Gly Gln Gly Phe Lys Arg Gly Lys Tyr Thr Gln Leu Val160 165 170ctc gtt caa gtg gaa acc ggg gaa atg aaa cag ctg acc gat ctt gaa1061Leu Val Gln Val Glu Thr Gly Glu Met Lys Gln Leu Thr Asp Leu Glu175 180 185cgc gac cat ttc agc cat gca ttt tca ccg tgc gga gat cag att gca1109Arg Asp His Phe Ser His Ala Phe Ser Pro Cys Gly Asp Gln Ile Ala190 195 200ttt tgc gcc aat caa acg gat atg cgt gtg aac gat gtc tac ttg atg1157Phe Cys Ala Asn Gln Thr Asp Met Arg Val Asn Asp Val Tyr Leu Met205 210 215aac ctt tcg aca ggt gat ttg aag cgc ctc aca ggt caa aac ggc att1205Asn Leu Ser Thr Gly Asp Leu Lys Arg Leu Thr Gly Gln Asn Gly Ile220 225 230 235ttt tct tca cta tcg ttt tct cct gac ggc aaa cac ctt gca ttt atc1253Phe Ser Ser Leu Ser Phe Ser Pro Asp Gly Lys His Leu Ala Phe Ile240 245 250gga aac gag aaa gaa tat caa aac gcc acg ctc gat aaa gca tgg ctg1301Gly Asn Glu Lys Glu Tyr Gln Asn Ala Thr Leu Asp Lys Ala Trp Leu255 260 265tat gat att gaa aca gga aaa cta acg tgt ctg acg gaa atg ctt gac1349
Tyr Asp Ile Glu Thr Gly Lys Leu Thr Cys Leu Thr Glu Met Leu Asp270 275 280gtc cat ctc agc gat gcg gtg gca gga gac agc ctt gtc gga ggc gtt1397Val His Leu Ser Asp Ala Val Ala Gly Asp Ser Leu Val Gly Gly Val285 290 295ctt ccg aaa ccg gcc tgg acc aaa gac gga aac ggc ttt tat gtc atc1445Leu Pro Lys Pro Ala Trp Thr Lys Asp Gly Asn Gly Phe Tyr Val Ile300 305 310 315gga tct gac cag ggt tct aca ggc att tac tac ata tca atc gaa ggt1493Gly Ser Asp Gln Gly Ser Thr Gly Ile Tyr Tyr Ile Ser Ile Glu Gly320 325 330ctt gct tat ccg gtc cgg ttg gaa aaa gag cat gtg aac gga ttc agc1541Leu Ala Tyr Pro Val Arg Leu Glu Lys Glu His Val Asn Gly Phe Ser335 340 345ctg cat ccg gac gaa agt ggc ttt gcg gca tcc ata gcc ctg ccc gtc1589Leu His Pro Asp Glu Ser Gly Phe Ala Ala Ser Ile Ala Leu Pro Val350 355 360cgg ccg agc gaa ctt tac cat ctc caa ttg ggt gaa gaa aaa gcg gag1637Arg Pro Ser Glu Leu Tyr His Leu Gln Leu Gly Glu Glu Lys Ala Glu365 370 375cga cta aca gat gcc aac caa gcg ttt acg gaa gaa cat atc ata tcg1685Arg Leu Thr Asp Ala Asn Gln Ala Phe Thr Glu Glu His Ile Ile Ser380 385 390 395gaa ccg gag gag ctt caa ttt cag gca gcg gac ggt ctg aca att cac1733Glu Pro Glu Glu Leu Gln Phe Gln Ala Ala Asp Gly Leu Thr Ile His400 405 410gga tgg ctg ata aag ccc gcc caa tat gaa aaa gga aac acg tat ccg1781Gly Trp Leu Ile Lys Pro Ala Gln Tyr Glu Lys Gly Asn Thr Tyr Pro415 420 425ctc att ctg gaa gtg cac ggc gga cct cac gcc atg tat gcg aat gct1829Leu Ile Leu Glu Val His Gly Gly Pro His Ala Met Tyr Ala Asn Ala430 435 440tat ttt cat gaa ttt caa gtg ctg gcc gca aag gga agc gcc gtc gta1877Tyr Phe His Glu Phe Gln Val Leu Ala Ala Lys Gly Ser Ala Val Val445 450 455tat gtt aac ccg cgg gga agc cac ggc tac gga cag gat ttt gtc aac1925Tyr Val Asn Pro Arg Gly Ser His Gly Tyr Gly Gln Asp Phe Val Asn460 465 470 475cgg gtg agg ggc gac tac ggc ggt gga gat ttt aag gat gta atg gcg1973Arg Val Arg Gly Asp Tyr Gly Gly Gly Asp Phe Lys Asp Val Met Ala480 485 490gct gtt gac cac gtg ctt gaa cat tat gat ttc gtc gat caa gaa agg2021Ala Val Asp His Val Leu Glu His Tyr Asp Phe Val Asp Gln Glu Arg495 500 505ctc ggc atc acc gga gga agc tac ggg ggc ttt atg aca aac tgg gct2069Leu Gly Ile Thr Gly Gly Ser Tyr Gly Gly Phe Met Thr Asn Trp Ala510 515 520gtc ggc cac acg aag cgc ttc aag gcc gca gtg aca cag agg tcc att2117Val Gly His Thr Lys Arg Phe Lys Ala Ala Val Thr Gln Arg Ser Ile525 530 535tcc aac tgg atc agt ttt tac ggg gta agc gac atc ggc tat ttt ttc2165Ser Asn Trp Ile Ser Phe Tyr Gly Val Ser Asp Ile Gly Tyr Phe Phe
540 545 550 555aca gac tgg cag ctc ggt gca gac ctt ttt gaa gat cca ggc aaa ctg2213Thr Asp Trp Gln Leu Gly Ala Asp Leu Phe Glu Asp Pro Gly Lys Leu560 565 570tgg gag cat tcg ccg ctc aaa tac gcg gac aag gtg gag acc cct ctt2261Trp Glu His Ser Pro Leu Lys Tyr Ala Asp Lys Val Glu Thr Pro Leu575 580 585ctc att ctg cat ggc gaa cgg gac gac agg tgc ccg att gaa cag gcg2309Leu Ile Leu His Gly Glu Arg Asp Asp Arg Cys Pro Ile Glu Gln Ala590 595 600gaa cag ctg ttc acg gcg ctg aaa aaa atg ggg aaa gaa gta aaa ctc2357Glu Gln Leu Phe Thr Ala Leu Lys Lys Met Gly Lys Glu Val Lys Leu605 610 615gtc aga ttt ccg aac gcg tca cac gat tta tca aga agc ggg cat ccg2405Val Arg Phe Pro Asn Ala Ser His Asp Leu Ser Arg Ser Gly His Pro620 625 630 635aag cag cgg atc agg cgg ctt gag tat att gca ggc tgg ttt gaa gct2453Lys Gln Arg Ile Arg Arg Leu Glu Tyr Ile Ala Gly Trp Phe Glu Ala640 645 650tat cta taaaaaagaa aaggctgccg tacactacgg cagcctcagc gtgtcgacaa 2509Tyr Leuaccctcgcat tcgttgtcag ggctgcgcgt cggtgctcac gaattccaac attcgctccg 2569ctccgatgct cgaccttcct agactgcaag ggttttcaat cacgctgaga ggatgacaaa 2629atcctaaact gaaaaccgtt ttaggatttt gtcaacaatt taggctgccg ttacggcagc 2689cttctcaaat taaaatactt gctctacttc aatgacgccc ggaacttctt ctaaaagggc 2749gcgctcaatt ccagctttaa gggtgatggt ggaacttggg cagcttccgc atgcgccgag 2809caggcgaagc ttgacgatgc cgtcttcaac atcgacgagt tcacagtcac cgccgtcacg 2869gagtaaaaac ggacgaagtt tgtccagcac ttcctgtact tggtccttca tttcgacctc 2929tgtcgccatt cgtatcgctc cttttaaat2958<210>8<211>653<212>PRT<213>地衣芽孢桿菌<400>8Met Lys Gln Leu Ile Thr Glu Lys Asp Leu Ile Lys Leu Val Ser Ile1 5 10 15Thr Asp Pro Gln Tyr Ser Pro Asp Gly Ala Lys Ile Ala Tyr Val Gln20 25 30Thr Lys Val Asn Glu Lys Gln Asp Ser Tyr Asp Ser His Ile Met Ile35 40 45Tyr Asp Arg Glu Lys Gln Ala Ser Val Gln Trp Thr Phe Gly Lys Gly50 55 60
Arg Asn Gln His Pro Arg Trp Ser Pro Asp Gly Lys Tyr Leu Ala Phe65 70 75 80Thr Ser Asn Arg Glu Glu Thr Ala Gln Ile Tyr Val Ile Ser Ala Ala85 90 95Gly Gly Glu Ala Arg Lys Val Thr Asp Ile Pro Tyr Asp Val Ser Gln100 105 110Pro Glu Trp Ser Pro Asp Gly Lys Ser Leu Leu Cys Ser Val Lys Leu115 120 125Thr Lys Glu Glu Ser Val Asp Asp Glu Lys Lys Thr Glu Ile Glu Asp130 135 140His Glu Pro Leu Glu Val Asp Ser Leu Ser Tyr Lys Ala Asp Gly Gln145 150 155 160Gly Phe Lys Arg Gly Lys Tyr Thr Gln Leu Val Leu Val Gln Val Glu165 170 175Thr Gly Glu Met Lys Gln Leu Thr Asp Leu Glu Arg Asp His Phe Ser180 185 190His Ala Phe Ser Pro Cys Gly Asp Gln Ile Ala Phe Cys Ala Asn Gln195 200 205Thr Asp Met Arg Val Asn Asp Val Tyr Leu Met Asn Leu Ser Thr Gly210 215 220Asp Leu Lys Arg Leu Thr Gly Gln Asn Gly Ile Phe Ser Ser Leu Ser225 230 235 240Phe Ser Pro Asp Gly Lys His Leu Ala Phe Ile Gly Asn Glu Lys Glu245 250 255Tyr Gln Asn Ala Thr Leu Asp Lys Ala Trp Leu Tyr Asp Ile Glu Thr260 265 270Gly Lys Leu Thr Cys Leu Thr Glu Met Leu Asp Val His Leu Ser Asp275 280 285Ala Val Ala Gly Asp Ser Leu Val Gly Gly Val Leu Pro Lys Pro Ala290 295 300Trp Thr Lys Asp Gly Asn Gly Phe Tyr Val Ile Gly Ser Asp Gln Gly305 310 315 320Ser Thr Gly Ile Tyr Tyr Ile Ser Ile Glu Gly Leu Ala Tyr Pro Val325 330 335
Arg Leu Glu Lys Glu His Val Asn Gly Phe Ser Leu His Pro Asp Glu340 345 350Ser Gly Phe Ala Ala Ser Ile Ala Leu Pro Val Arg Pro Ser Glu Leu355 360 365Tyr His Leu Gln Leu Gly Glu Glu Lys Ala Glu Arg Leu Thr Asp Ala370 375 380Asn Gln Ala Phe Thr Glu Glu His Ile Ile Ser Glu Pro Glu Glu Leu385 390 395 400Gln Phe Gln Ala Ala Asp Gly Leu Thr Ile His Gly Trp Leu Ile Lys405 410 415Pro Ala Gln Tyr Glu Lys Gly Asn Thr Tyr Pro Leu Ile Leu Glu Val420 425 430His Gly Gly Pro His Ala Met Tyr Ala Asn Ala Tyr Phe His Glu Phe435 440 445Gln Val Leu Ala Ala Lys Gly Ser Ala Val Val Tyr Val Asn Pro Arg450 455 460Gly Ser His Gly Tyr Gly Gln Asp Phe Val Asn Arg Val Arg Gly Asp465 470 475 480Tyr Gly Gly Gly Asp Phe Lys Asp Val Met Ala Ala Val Asp His Val485 490 495Leu Glu His Tyr Asp Phe Val Asp Gln Glu Arg Leu Gly Ile Thr Gly500 505 5l0Gly Ser Tyr Gly Gly Phe Met Thr Asn Trp Ala Val Gly His Thr Lys515 520 525Arg Phe Lys Ala Ala Val Thr Gln Arg Ser Ile Ser Asn Trp Ile Ser530 535 540Phe Tyr Gly Val Ser Asp Ile Gly Tyr Phe Phe Thr Asp Trp Gln Leu545 550 555 560Gly Ala Asp Leu Phe Glu Asp Pro Gly Lys Leu Trp Glu His Ser Pro565 570 575Leu Lys Tyr Ala Asp Lys Val Glu Thr Pro Leu Leu Ile Leu His Gly580 585 590Glu Arg Asp Asp Arg Cys Pro Ile Glu Gln Ala Glu Gln Leu Phe Thr595 600 605Ala Leu Lys Lys Met Gly Lys Glu Val Lys Leu Val Arg Phe Pro Asn
610 615 620Ala Ser His Asp Leu Ser Arg Ser Gly His Pro Lys Gln Arg Ile Arg625 630 635 640Arg Leu Glu Tyr Ile Ala Gly Trp Phe Glu Ala Tyr Leu645 650<210>9<211>2473<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(148)..(1974)<400>9tatgtatttt attttttaac ggcgggattt tgtgtctttt ttttcgcata tgatttgaca 60aaagacggtg aaaaaacaga agatgaacac aatacaagtt tattggataa caatggactt 120ggagtaaaga aagcgagggg attgaca gtg gct gaa gaa aaa aaa tcg aag aaa 174Val Ala Glu Glu Lys Lys Ser Lys Lys1 5ctg cct tca aga gaa gag gtg aag caa gaa gac acg tgg aga ctg gag222Leu Pro Ser Arg Glu Glu Val Lys Gln Glu Asp Thr Trp Arg Leu Glu10 15 20 25gat atc ttc ccg tca gat gac gct tgg aac gag gaa ttt caa gcc gtt270Asp Ile Phe Pro Ser Asp Asp Ala Trp Asn Glu Glu Phe Gln Ala Val30 35 40aaa gaa ctg ctg ccc aag ctt tcc gaa ttc aag ggc aag ctc ggc cat318Lys Glu Leu Leu Pro Lys Leu Ser Glu Phe Lys Gly Lys Leu Gly His45 50 55tcc gcc gat gat tta tat gaa gcg ctg act tat cag gat aag gtg atg366Ser Ala Asp Asp Leu Tyr Glu Ala Leu Thr Tyr Gln Asp Lys Val Met60 65 70gag cgt ctc ggg aag ctg tat act tac gct cat atg cgc tat gat cag414G1u Arg Leu Gly Lys Leu Tyr Thr Tyr Ala His Met Arg Tyr Asp Gln75 80 85gat acg gga aat tct ttt tac caa ggt ctc aat gat aaa gcg gcg aac462Asp Thr Gly Asn Ser Phe Tyr Gln Gly Leu Asn Asp Lys Ala Ala Asn90 95 100 105ctc tat act cag gct gcg agc gcc acg gcc tac atg gtg ccg gaa ata510Leu Tyr Thr Gln Ala Ala Ser Ala Thr Ala Tyr Met Val Pro Glu Ile110 115 120tta tcg att cag gaa gaa aag ctt cag cag ttc ctg ttg gaa aaa gag558Leu Ser Ile Gln Glu Glu Lys Leu Gln Gln Phe Leu Leu Glu Lys Glu125 130 135gaa tta aag ctt tac tca cac gct ctc gaa gaa atc aat aaa gag cgc606Glu Leu Lys Leu Tyr Ser His Ala Leu Glu Glu Ile Asn Lys Glu Arg140 145 150cct cat gtg ctt agc gag gaa gag gaa ggg att ttg gcc gaa gcg tct654Pro His Val Leu Ser Glu Glu Glu Glu Gly Ile Leu Ala Glu Ala Ser
155 160 165gat gtt ctt tca tct cct tcc aat aca ttc ggc atg ctg aat aac gcc 702Asp Val Leu Ser Ser Pro Ser Asn Thr Phe Gly Met Leu Asn Asn Ala170 175 180 185gat atg gaa ttt ccg gag atc acc gat gaa aac gga gaa aag gtg cag 750Asp Met Glu Phe Pro Glu Ile Thr Asp Glu Asn Gly Glu Lys Val Gln190 195 200ctc aca cac ggc aat tat atc acc ttt ttg gaa agc gag aac cgc gat 798Leu Thr His Gly Asn Tyr Ile Thr Phe Leu Glu Ser Glu Asn Arg Asp205 210 215gtc cgc cgc gcg gca ttc aag gcc gtt tat gaa aca tac ggc cgc ttt 846Val Arg Arg Ala Ala Phe Lys Ala Val Tyr Glu Thr Tyr Gly Arg Phe220 225 230aaa aac acg cta gct tcc acg ctc agc gga gct gtg aaa aaa gac aac 894Lys Asn Thr Leu Ala Ser Thr Leu Ser Gly Ala Val Lys Lys Asp Asn235 240 245ttt tat gcg aag gtt aaa cat tat aaa tcg gcg cgg gaa gct gcc ttg 942Phe Tyr Ala Lys Val Lys His Tyr Lys Ser Ala Arg Glu Ala Ala Leu250 255 260 265tcg cgc aac agc att cct gaa gaa gtc tac gac aat ctt gtc agc acg 990Ser Arg Asn Ser Ile Pro Glu Glu Val Tyr Asp Asn Leu Val Ser Thr270 275 280atc aac aag cat ctc ccg ctt ctt cac cgc tat gtt gag att aga aaa1038Ile Asn Lys His Leu Pro Leu Leu His Arg Tyr Val Glu Ile Arg Lys285 290 295aaa gtg ctt gag ctt gat gaa gtg cac atg tat gac ctg tat acc cct1086Lys Val Leu Glu Leu Asp Glu Val His Met Tyr Asp Leu Tyr Thr Pro300 305 310ctg gtc aag gat tct gga atg aaa gtc act tac gag cag gca aag gac1134Leu Val Lys Asp Ser Gly Met Lys Val Thr Tyr Glu Gln Ala Lys Asp315 320 325tat atg ctg aag ggt ctg gcg cct ttg ggg gaa gaa tat tca tcc atc1182Tyr Met Leu Lys Gly Leu Ala Pro Leu Gly Glu Glu Tyr Ser Ser Ile330 335 340 345ctg aaa gaa gga ttg aac aac cgc tgg gtc gat gtg tat gaa aac aaa1230Leu Lys Glu Gly Leu Asn Asn Arg Trp Val Asp Val Tyr Glu Asn Lys350 355 360gga aaa cga agc ggc gcc tat tct tca gga act tac gga acc aat ccg1278Gly Lys Arg Ser Gly Ala Tyr Ser Ser Gly Thr Tyr Gly Thr Asn Pro365 370 375tat att ttg atg aac tgg cag gac aac gtc aac aat ctg ttt acg ctc1326Tyr Ile Leu Met Asn Trp Gln Asp Asn Val Asn Asn Leu Phe Thr Leu380 385 390gtc cat gaa ttc ggc cat tcc gtg cac agc tac tat acg aga aaa tat1374Val His Glu Phe Gly His Ser Val His Ser Tyr Tyr Thr Arg Lys Tyr395 400 405cag cct tac ccg tac ggt aac tac agc att ttc gta gcc gaa gtt gct1422Gln Pro Tyr Pro Tyr Gly Asn Tyr Ser Ile Phe Val Ala Glu Val Ala410 415 420 425tct acg acg aat gaa gcg ctg ttg gga gaa tac ctg ctg aat aca atc1470
Ser Thr Thr Asn Glu Ala Leu Leu Gly Glu Tyr Leu Leu Asn Thr Ile430 435 440gaa gat gaa aaa cag cgg ctc tat att ttg aat cat atg ctc gaa ggc1518Glu Asp Glu Lys Gln Arg Leu Tyr Ile Leu Asn His Met Leu Glu Gly445 450 455ttt aaa gga acg gtt ttc aga cag acg atg ttt gcc gaa ttt gaa cat1566Phe Lys Gly Thr Val Phe Arg Gln Thr Met Phe Ala Glu Phe Glu His460 465 470gag atc cat gtc aaa gcc cag gaa ggg gaa ccg ctg aca cct gag ctg1614Glu Ile His Val Lys Ala Gln Glu Gly Glu Pro Leu Thr Pro Glu Leu475 480 485tta acg agc ata tac tat gat ttg aat aag aaa tat ttc gga gac aat1662Leu Thr Ser Ile Tyr Tyr Asp Leu Asn Lys Lys Tyr Phe Gly Asp Asn490 495 500 505att gag att gat aag gaa atc ggt ctt gag tgg tca aga att ccg cat1710Ile Glu Ile Asp Lys Glu Ile Gly Leu Glu Trp Ser Arg Ile Pro His510 515 520ttc tat tac aat tat tat gta tat cag tat gcg aca ggc ttc agt gca1758Phe Tyr Tyr Asn Tyr Tyr Val Tyr Gln Tyr Ala Thr Gly Phe Ser Ala525 530 535gct cag gcg ctc agc cag cag att tta aaa gaa ggc aaa tcg gct gtt1806Ala Gln Ala Leu Ser Gln Gln Ile Leu Lys Glu Gly Lys Ser Ala Val540 545 550gac cgc tat att gag ttc ttg aaa gct gga agc tcg gat tat ccg att1854Asp Arg Tyr Ile Glu Phe Leu Lys Ala Gly Ser Ser Asp Tyr Pro Ile555 560 565gaa gtg ctg aaa aaa gcc ggc gtc gat atg acg tct tca gag ccg atc1902Glu Val Leu Lys Lys Ala Gly Val Asp Met Thr Ser Ser Glu Pro Ile570 575 580 585gaa gct gcg tgc aaa aag ttt gaa gag cag ctg aat gaa atg gaa gaa1950Glu Ala Ala Cys Lys Lys Phe Glu Glu Gln Leu Asn Glu Met Glu Glu590 595 600ctc ctg caa aaa gtc aat cat tca taaaaggcgt atgcatgaaa gaggctgccg 2004Leu Leu Gln Lys Val Asn His Ser605gtctaccggc agccttgttc gcgatgatgt caaaaacctt taaacgtttt gcggtggttg 2064aggcggctga tcgtaaaaag gattgatata aataataggg gagacgtaat ataaaggggt 2124agcatattca tcaaacctaa aatattcaag acgaaaaaaa ataaaattga tgcaagtccc 2184aaaatgatct gaagcataaa aaccctccta aggactgata aaatattaag ggggcgacga 2244agcgcaataa gaccggcaaa accgggcgcc agtccgcata attcggggtt tggcagcgtg 2304cgtcgggctc gttttctttg ttactttgat catatgatgg acatctatta tttatgtttt 2364tgtgacaaaa atgt gaaaac taatgaaatc agttgtcaaa actcgacatg atttgatatt 2424ataaagatgt gaaattgatc acaaacaaac atttacccct ttgtttgac 2473<210>10<211>609
<212>PRT<213>地衣芽孢桿菌<400>10Val Ala Glu Glu Lys Lys Ser Lys Lys Leu Pro Ser Arg Glu Glu Val1 5 10 15Lys Gln Glu Asp Thr Trp Arg Leu Glu Asp Ile Phe Pro Ser Asp Asp20 25 30Ala Trp Asn Glu Glu Phe Gln Ala Val Lys Glu Leu Leu Pro Lys Leu35 40 45Ser Glu Phe Lys Gly Lys Leu Gly His Ser Ala Asp Asp Leu Tyr Glu50 55 60Ala Leu Thr Tyr Gln Asp Lys Val Met Glu Arg Leu Gly Lys Leu Tyr65 70 75 80Thr Tyr Ala His Met Arg Tyr Asp Gln Asp Thr Gly Asn Ser Phe Tyr85 90 95Gln Gly Leu Asn Asp Lys Ala Ala Asn Leu Tyr Thr Gln Ala Ala Ser100 105 110Ala Thr Ala Tyr Met Val Pro Glu Ile Leu Ser Ile Gln Glu Glu Lys115 120 125Leu Gln Gln Phe Leu Leu Glu Lys Glu Glu Leu Lys Leu Tyr Ser His130 135 140Ala Leu Glu Glu Ile Asn Lys Glu Arg Pro His Val Leu Ser Glu Glu145 150 155 160Glu Glu Gly Ile Leu Ala Glu Ala Ser Asp Val Leu Ser Ser Pro Ser165 170 175Asn Thr Phe Gly Met Leu Asn Asn Ala Asp Met Glu Phe Pro Glu Ile180 185 190Thr Asp Glu Asn Gly Glu Lys Val Gln Leu Thr His Gly Asn Tyr Ile195 200 205Thr Phe Leu Glu Ser Glu Asn Arg Asp Val Arg Arg Ala Ala Phe Lys210 215 220Ala Val Tyr Glu Thr Tyr Gly Arg Phe Lys Asn Thr Leu Ala Ser Thr225 230 235 240Leu Ser Gly Ala Val Lys Lys Asp Asn Phe Tyr Ala Lys Val Lys His245 250 255
Tyr Lys Ser Ala Arg Glu Ala Ala Leu Ser Arg Asn Ser Ile Pro Glu260 265 270Glu Val Tyr Asp Asn Leu Val Ser Thr Ile Asn Lys His Leu Pro Leu275 280 285Leu His Arg Tyr Val Glu Ile Arg Lys Lys Val Leu Glu Leu Asp Glu290 295 300Val His Met Tyr Asp Leu Tyr Thr Pro Leu Val Lys Asp Ser Gly Met305 3I0 315 320Lys Val Thr Tyr Glu Gln Ala Lys Asp Tyr Met Leu Lys Gly Leu Ala325 330 335Pro Leu Gly Glu Glu Tyr Ser Ser Ile Leu Lys Glu Gly Leu Asn Asn340 345 350Arg Trp Val Asp Val Tyr Glu Asn Lys Gly Lys Arg Ser Gly Ala Tyr355 360 365Ser Ser Gly Thr Tyr Gly Thr Asn Pro Tyr Ile Leu Met Asn Trp Gln370 375 380Asp Asn Val Asn Asn Leu Phe Thr Leu Val His Glu Phe Gly His Ser385 390 395 400Val His Ser Tyr Tyr Thr Arg Lys Tyr Gln Pro Tyr Pro Tyr Gly Asn405 410 415Tyr Ser Ile Phe Val Ala Glu Val Ala Ser Thr Thr Asn Glu Ala Leu420 425 430Leu Gly Glu Tyr Leu Leu Asn Thr Ile Glu Asp Glu Lys Gln Arg Leu435 440 445Tyr Ile Leu Asn His Met Leu Glu Gly Phe Lys Gly Thr Val Phe Arg450 455 460Gln Thr Met Phe Ala Glu Phe Glu His Glu Ile His Val Lys Ala Gln465 470 475 480Glu Gly Glu Pro Leu Thr Pro Glu Leu Leu Thr Ser Ile Tyr Tyr Asp485 490 495Leu Asn Lys Lys Tyr Phe Gly Asp Asn Ile Glu Ile Asp Lys Glu Ile500 505 510Gly Leu Glu Trp Ser Arg Ile Pro His Phe Tyr Tyr Asn Tyr Tyr Val515 520 525Tyr Gln Tyr Ala Thr Gly Phe Ser Ala Ala Gln Ala Leu Ser Gln Gln
530 535 540Ile Leu Lys Glu Gly Lys Ser Ala Val Asp Arg Tyr lle Glu Phe Leu545 550 555 560Lys Ala Gly Ser Ser Asp Tyr Pro Ile Glu Val Leu Lys Lys Ala Gly565 570 575Val Asp Met Thr Ser Ser Glu Pro Ile Glu Ala Ala Cys Lys Lys Phe580 585 590Glu Glu Gln Leu Asn Glu Met Glu Glu Leu Leu Gln Lys Val Asn His595 600 605Ser<210>11<211>2746<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(491)..(2245)<400>11atctcctttt gtctcatact cttttaaggt atccgcgaat tctttaaggg ttgtctgatt 60cgtcacttta tagttaacaa aatactcagc tgtttcatct aaattttttg caatttccgt 120caaaagcacg gaaaacttgt ctttttttct tttcaacatt tgtaaccctc cattgtttaa 180atgagacaat gcacacatca aaatattata taactttttg tcgaaaaaaa acaggtgcag 240tcgaaaaaaa caattaattt tcaaatcgtc atacctttta gataggcgca agcaccagct 300ggcatccggg attttaagat gacaataggc gatttttcct ttgatatcat cgttccaaaa 360gaacggatct caggatcgtt tcgagaaatt tgggagttta ggttcaaaaa aatagaaaat 420tgggagtttt tcttattttc agtccgcttt cctgttatag tagtaataaa ctggaaagga 480aggtgatctg atg aga cgg tta gct ttc tta gtt gtt gcg ttt tgc ttg 529Met Arg Arg Leu Ala Phe Leu Val Val Ala Phe Cys Leu1 5 10gcg gtt ggt tgc ttt ttc agt ccg gtg tcg aaa gca gaa gga gtc atg577Ala Val Gly Cys Phe Phe Ser Pro Val Ser Lys Ala Glu Gly Val Met15 20 25agc ggg gga gat ggt gat aaa gtc gcc gtc ggg aaa gac ggt atg gtg625Ser Gly Gly Asp Gly Asp Lys Val Ala Val Gly Lys Asp Gly Met Val30 35 40 45gct acg gcc cat ccg ctt gca tca aaa atc gga gcc gaa gtt ttg aaa673Ala Thr Ala His Pro Leu Ala Ser Lys Ile Gly Ala Glu Val Leu Lys50 55 60aag ggc ggg aat gcc att gat gca gct att gcg atc cag tac gca ctg721Lys Gly Gly Asn Ala Ile Asp Ala Ala Ile Ala Ile Gln Tyr Ala Leu65 70 75
aat gta aca gag ccg atg atg tct gga atc ggc ggc ggc ggt ttc atg 769Asn Val Thr Glu Pro Met Met Ser Gly Ile Gly Gly Gly Gly Phe Met80 85 90atg gtc tac gac ggc gag acg aag gaa acg tcg atc atc aac agc agg 817Met Val Tyr Asp Gly Glu Thr Lys Glu Thr Ser Ile Ile Asn Ser Arg95 100 105gag cgt gct ccg gag gga gcc aag cct gac atg ttt ttg gat gaa gac 865Glu Arg Ala Pro Glu Gly Ala Lys Pro Asp Met Phe Leu Asp Glu Asp110 115 120 125gga aag gtg att ccg ttt tca gaa agg tcg aga cac gga aat gcg gtc 913Gly Lys Val Ile Pro Phe Ser Glu Arg Ser Arg His Gly Asn Ala Val130 135 140ggt gtg ccg gga acg ctg aaa ggg ctt gaa gca gcg cat aag aag tgg 961Gly Val Pro Gly Thr Leu Lys Gly Leu Glu Ala Ala His Lys Lys Trp145 150 155gga aca aag aaa atg gaa gac ttg att tcg cct tct ata aag ctt gca1009Gly Thr Lys Lys Met Glu Asp Leu Ile Ser Pro Ser Ile Lys Leu Ala160 165 170gag gaa ggg ttc cca atc gac tcg gtt ttg gct gac gcg ata aag gat1057Glu Glu Gly Phe Pro Ile Asp Ser Val Leu Ala Asp Ala Ile Lys Asp175 180 185cat cag gac aag ctg agc aaa acg gcc gca aag gat att ttc ctt ccg1105His Gln Asp Lys Leu Ser Lys Thr Ala Ala Lys Asp Ile Phe Leu Pro190 195 200 205gac ggt gaa cct ttg aaa gaa ggc gat atc ctt gta caa aag gat ttg1153Asp Gly Glu Pro Leu Lys Glu Gly Asp Ile Leu Val Gln Lys Asp Leu210 215 220gcc aaa acg ttt aaa ttg ata cgg aaa gaa ggg tca aaa gcc ttt tat1201Ala Lys Thr Phe Lys Leu Ile Arg Lys Glu Gly Ser Lys Ala Phe Tyr225 230 235gat ggg gaa atc ggc cgg gcg att gca gat gtc gtg cag gat ttc ggg1249Asp Gly Glu Ile Gly Arg Ala Ile Ala Asp Val Val Gln Asp Phe Gly240 245 250ggt tca atg aca cct gat gac ctg tcc cgc tac gag gtc aca aca gat1297Gly Ser Met Thr Pro Asp Asp Leu Ser Arg Tyr Glu Val Thr Thr Asp255 260 265aag ccc atc tgg ggc gag tac cac ggc tat gat att gca agc atg cct1345Lys Pro Ile Trp Gly Glu Tyr His Gly Tyr Asp Ile Ala Ser Met Pro270 275 280 285cct cca agc tca ggc gga gtg ttc atg ctg cag atg ctg aaa ttg att1393Pro Pro Ser Ser Gly Gly Val Phe Met Leu Gln Met Leu Lys Leu Ile290 295 300gat gat ttt cat tta tcg caa tac gat cca aag tcg ttt gaa aaa tat1441Asp Asp Phe His Leu Ser Gln Tyr Asp Pro Lys Ser Phe Glu Lys Tyr305 310 315cat ctg ctc gcg gaa acg atg cat ctc tcc tat gcg gac agg gct gca1489His Leu Leu Ala Glu Thr Met His Leu Ser Tyr Ala Asp Arg Ala Ala320 325 330tat gcg ggc gat ccg gaa ttt gtc gat gtt ccg ctc aga ggg ctg ctc1537Tyr Ala Gly Asp Pro Glu Phe Val Asp Val Pro Leu Arg Gly Leu Leu335 340 345
gat ccc gat tat att aag gag cgg caa aag ctg atc agc ctt gat tcg 1585Asp Pro Asp Tyr Ile Lys Glu Arg Gln Lys Leu Ile Ser Leu Asp Ser350 355 360 365atg aac cgc gat gtg aaa gag ggc gat ccg tgg aaa tat gag gaa ggc 1633Met Asn Arg Asp Val Lys Glu Gly Asp Pro Trp Lys Tyr Glu Glu Gly370 375 380gag ccg aat tat gag atc gtg cct cag cct gaa gat aaa acg atc ggg 1681Glu Pro Asn Tyr Glu Ile Val Pro Gln Pro Glu Asp Lys Thr Ile Gly385 390 395gag acg acg cat ttt acc gtc acg gat cag tgg ggc aat gtt gtc tct 1729Glu Thr Thr His Phe Thr Val Thr Asp Gln Trp Gly Asn Val Val Ser400 405 410tat acg aca acg att gaa cag ctt ttt gga acg ggg att tta gtg ccc 1777Tyr Thr Thr Thr Ile Glu Gln Leu Phe Gly Thr Gly Ile Leu Val Pro415 420 425ggg tac gga ctg ttt ttg aac aat gaa ctg acc gat ttt gat gcg att 1825Gly Tyr Gly Leu Phe Leu Asn Asn Glu Leu Thr Asp Phe Asp Ala Ile430 435 440 445cca ggc ggc gcc aac gaa gtg cag ccg aat aaa agg ccg ctt tca agc 1873Pro Gly Gly Ala Asn Glu Val Gln Pro Asn Lys Arg Pro Leu Ser Ser450 455 460atg acg ccg acg atc gta ttc aaa gat gaa aaa ccg gtg ctg act gtc 1921Met Thr Pro Thr Ile Val Phe Lys Asp Glu Lys Pro Val Leu Thr Val465 470 475ggt tcc cct ggc gga acg acg atc att gct tcc gtt ttc cag acg atc 1969Gly Ser Pro Gly Gly Thr Thr Ile Ile Ala Ser Val Phe Gln Thr Ile480 485 490ttg aat tat ttt gag tac ggc atg agc ctt cag gat gcg att gaa gag 2017Leu Asn Tyr Phe Glu Tyr Gly Met Ser Leu Gln Asp Ala Ile Glu Glu495 500 505ccg agg atc tat acg aac agc ctg act tcg tac cgc tat gaa tcc gga 2065Pro Arg Ile Tyr Thr Asn Ser Leu Thr Ser Tyr Arg Tyr Glu Ser Gly510 515 520 525atg ccg gag gat gtc aga cgg aag ctc aat gac ttc ggc cac aaa ttc 2113Met Pro Glu Asp Val Arg Arg Lys Leu Asn Asp Phe Gly His Lys Phe530 535 540ggc tcg aat ccg gtt gat atc ggc aat gtc caa agc att ttc att gac 2161Gly Ser Asn Pro Val Asp Ile Gly Asn Val Gln Ser Ile Phe Ile Asp545 550 555cgg gag aac aaa acg ttt atg ggg gtt gcc gat tca agc aga aac gga 2209Arg Glu Asn Lys Thr Phe Met Gly Val Ala Asp Ser Ser Arg Asn Gly560 565 570acg gcg gtt ggg gtc aac att aag aca tcg gct aaa tagcggagca 2255Thr Ala Val Gly Val Asn Ile Lys Thr Ser Ala Lys575 580 585aggcttggtc taaagtattt agatcaagcc ttcttccgtc agcggggaaa agaggatttt2315tttcgaaaga atagaaatat attttttgaa agcgtttacc aatttataaa ggaggggtac2375catgattcac acgttggcaa atggatttta ccgcttttgc gagtgggtga tgcggcttgc2435ctatttgaat ctgctgtgga tcggctttac gctggcggga gcggtcatct tcggtttagc2495
gccggcgacc gccgcgatgt tcgctgtgac tagacagtgg acgctgggaa aaacggatgt 2555cccggtgttt cagacgtttt ttcgggcgtt taaaaagaaa tgggccaaat caagcgttct 2615cggtttgata ttgggtctgg tcggcttatt gctgtttgtt gattttcaga ttgcggccgt 2675ctattttcac gatcagccag tgtttttaag cctgttcatc agtttgttta tcatttatgc 2735catcatcctc t 2746<210>12<211>585<212>PRT<213>地衣芽孢桿菌<400>12Met Arg Arg Leu Ala Phe Leu Val Val Ala Phe Cys Leu Ala Val Gly1 5 10 15Cys Phe Phe Ser Pro Val Ser Lys Ala Glu Gly Val Met Ser Gly Gly20 25 30Asp Gly Asp Lys Val Ala Val Gly Lys Asp Gly Met Val Ala Thr Ala35 40 45His Pro Leu Ala Ser Lys Ile Gly Ala Glu Val Leu Lys Lys Gly Gly50 55 60Asn Ala Ile Asp Ala Ala Ile Ala Ile Gln Tyr Ala Leu Asn Val Thr65 70 75 80Glu Pro Met Met Ser Gly Ile Gly Gly Gly Gly Phe Met Met Val Tyr85 90 95Asp Gly Glu Thr Lys Glu Thr Ser Ile Ile Asn Ser Arg Glu Arg Ala100 105 110Pro Glu Gly Ala Lys Pro Asp Met Phe Leu Asp Glu Asp Gly Lys Val115 120 125Ile Pro Phe Ser Glu Arg Ser Arg His Gly Asn Ala Val Gly Val Pro130 135 140Gly Thr Leu Lys Gly Leu Glu Ala Ala His Lys Lys Trp Gly Thr Lys145 150 155 160Lys Met Glu Asp Leu Ile Ser Pro Ser Ile Lys Leu Ala Glu Glu Gly165 170 175Phe Pro Ile Asp Ser Val Leu Ala Asp Ala Ile Lys Asp His Gln Asp180 185 190Lys Leu Ser Lys Thr Ala Ala Lys Asp Ile Phe Leu Pro Asp Gly Glu195 200 205
Pro Leu Lys Glu Gly Asp Ile Leu Val Gln Lys Asp Leu Ala Lys Thr210 215 220Phe Lys Leu Ile Arg Lys Glu Gly Ser Lys Ala Phe Tyr Asp Gly Glu225 230 235 240Ile Gly Arg Ala Ile Ala Asp Val Val Gln Asp Phe Gly Gly Ser Met245 250 255Thr Pro Asp Asp Leu Ser Arg Tyr Glu Val Thr Thr Asp Lys Pro Ile260 265 270Trp Gly Glu Tyr His Gly Tyr Asp Ile Ala Ser Met Pro Pro Pro Ser275 280 285Ser Gly Gly Val Phe Met Leu Gln Met Leu Lys Leu Ile Asp Asp Phe290 295 300His Leu Ser Gln Tyr Asp Pro Lys Ser Phe Glu Lys Tyr His Leu Leu305 310 315 320Ala Glu Thr Met His Leu Ser Tyr Ala Asp Arg Ala Ala Tyr Ala Gly325 330 335Asp Pro Glu Phe Val Asp Val Pro Leu Arg Gly Leu Leu Asp Pro Asp340 345 350Tyr Ile Lys Glu Arg Gln Lys Leu Ile Ser Leu Asp Ser Met Asn Arg355 360 365Asp Val Lys Glu Gly Asp Pro Trp Lys Tyr Glu Glu Gly Glu Pro Asn370 375 380Tyr Glu Ile Val Pro Gln Pro Glu Asp Lys Thr Ile Gly Glu Thr Thr385 390 395 400His Phe Thr Val Thr Asp Gln Trp Gly Asn Val Val Ser Tyr Thr Thr405 410 415Thr Ile Glu Gln Leu Phe Gly Thr Gly Ile Leu Val Pro Gly Tyr Gly420 425 430Leu Phe Leu Asn Asn Glu Leu Thr Asp Phe Asp Ala Ile Pro Gly Gly435 440 445Ala Asn Glu Val Gln Pro Asn Lys Arg Pro Leu Ser Ser Met Thr Pro450 455 460Thr Ile Val Phe Lys Asp Glu Lys Pro Val Leu Thr Val Gly Ser Pro465 470 475 480
Gly Gly Thr Thr Ile Ile Ala Ser Val Phe Gln Thr Ile Leu Asn Tyr485 490 495Phe Glu Tyr Gly Met Ser Leu Gln Asp Ala Ile Glu Glu Pro Arg Ile500 505 510Tyr Thr Asn Ser Leu Thr Ser Tyr Arg Tyr Glu Ser Gly Met Pro Glu515 520 525Asp Val Arg Arg Lys Leu Asn Asp Phe Gly His Lys Phe Gly Ser Asn530 535 540Pro Val Asp Ile Gly Asn Val Gln Ser Ile Phe Ile Asp Arg Glu Asn545 550 555 560Lys Thr Phe Met Gly Val Ala Asp Ser Ser Arg Asn Gly Thr Ala Val565 570 575Gly Val Asn Ile Lys Thr Ser Ala Lys580 585<210>13<211>2314<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(493)..(1818)<400>13ctgaaatcta tcaaggcaga cgctttaaag tttatcgcgg catgggttct gtagctgcga60tggaaaaagg aagcaaagac cgctacttcc aagaggaaaa caaaaaattc gttcctgaag 120gcattgaagg ccgtacgcct tataaaggcc ctgttgctga taccgtttat cagcttgtcg 180gcggcttgaa atccggcatg ggctactgcg gaacgaaaga tttgcacgca ttgagggaag 240atgcgcagtt catccgcatg acaggagcag gcctgcggga aagccatcct cacgatgtgc 300aaattaccaa agaatcgcct aactatacca tttcctaatc aaatatgaca aaaattaaac 360cgtagacaga gtgatactct gtctattttt ttttgcttca ttatgataaa atttataatg 420ttgtgtttga aaaatgtctt aaaaggcttt gatatagatt attagtctga tgaaaagcgg 480aggtatcacg at ttg aaa agc aag aga tta aag cag ctg atc atg tta atc 531Leu Lys Ser Lys Arg Leu Lys Gln Leu Ile Met Leu Ile1 5 10gtt gct ttt gcc gtg act gta ggt gca ttt tct cct atg tca act gca 579Val Ala Phe Ala Val Thr Val Gly Ala Phe Ser Pro Met Ser Thr Ala15 20 25aaa gct gca aat gat cca atc aat gta gac gcg aaa gca gca arc ctg 627Lys Ala Ala Asn Asp Pro Ile Asn Val Asp Ala Lys Ala Ala Ile Leu30 35 40 45gtg gaa gct tca tcg ggg aaa att ttg tac agt aaa aat gcc gac cag 675Val Glu Ala Ser Ser Gly Lys Ile Leu Tyr Ser Lys Asn Ala Asp Gln
50 55 60cgt ctt cct gtt gcc agc atg gca aaa atg atg acg gag tat ctt ctg 723Arg Leu Pro Val Ala Ser Met Ala Lys Met Met Thr Glu Tyr Leu Leu65 70 75ttg gaa gcg att cac gaa ggc aaa gtg aaa tgg gat cag aag tac acg 771Leu Glu Ala Ile His Glu Gly Lys Val Lys Trp Asp Gln Lys Tyr Thr80 85 90cct gac gat tat gta tac gag att tcc caa gat cgt tca cta tcc aat 819Pro Asp Asp Tyr Val Tyr Glu Ile Ser Gln Asp Arg Ser Leu Ser Asn95 100 105gtt cct ctt cgt aaa gac gga tca tac act gta aaa gag ctt tat cag 867Val Pro Leu Arg Lys Asp Gly Ser Tyr Thr Val Lys Glu Leu Tyr Gln110 115 120 125gcg act gca att tac tct gca aac gcg gcg gct att gca ctt tct gaa 915Ala Thr Ala Ile Tyr Ser Ala Asn Ala Ala Ala Ile Ala Leu Ser Glu130 135 140att gtt gcc ggt tct gaa tcg aag ttt gta gag tta atg aac aaa aaa 963Ile Val Ala Gly Ser Glu Ser Lys Phe Val Glu Leu Met Asn Lys Lys145 150 155gcc aaa gag ctt gga atg aaa gat ttc aaa ttt gtg aat gcc aca ggt1011Ala Lys Glu Leu Gly Met Lys Asp Phe Lys Phe Val Asn Ala Thr Gly160 165 170ttg gaa aac aag gat ctt cac ggc aaa cat ccg agc gga aca aat gcc1059Leu Glu Asn Lys Asp Leu His Gly Lys His Pro Ser Gly Thr Asn Ala175 180 185aat gaa gaa aat gaa gtg tct gcg aga gac atg gcg ctc ctt gcc gat1107Asn Glu Glu Asn Glu Val Ser Ala Arg Asp Met Ala Leu Leu Ala Asp190 195 200 205cat tta gtg agc gat tat cca gag atc ctc gat acg gcg agc att gcc1155His Leu Val Ser Asp Tyr Pro Glu Ile Leu Asp Thr Ala Ser Ile Ala210 215 220aaa acg aag ttc aga aaa ggc aca gac gat gaa atg gat atg ccg aac1203Lys Thr Lys Phe Arg Lys Gly Thr Asp Asp Glu Met Asp Met Pro Asn225 230 235tgg aac ttt atg ctg aaa ggc ctt gtt cag caa tat gaa ggt gtg gac1251Trp Asn Phe Met Leu Lys Gly Leu Val Gln Gln Tyr Glu Gly Val Asp240 245 250ggg ctg aaa acc ggg tca acc gac tca gcc gga tca tgc ttt acg gct1299Gly Leu Lys Thr Gly Ser Thr Asp Ser Ala Gly Ser Cys Phe Thr Ala255 260 265act gct gaa aga aac ggc atg aga gtg att tcc gtc gtt ctg aat gcg1347Thr Ala Glu Arg Asn Gly Met Arg Val Ile Ser Val Val Leu Asn Ala270 275 280 285aaa ggc gac ctt cat acg gcg cga ttt aaa gaa acg aaa aaa atg ctc1395Lys Gly Asp Leu His Thr Ala Arg Phe Lys Glu Thr Lys Lys Met Leu290 295 300gat tat gca ttt gaa aac ttc act atg aaa gag ttt tac gca aaa ggc1443Asp Tyr Ala Phe Glu Asn Phe Thr Met Lys Glu Phe Tyr Ala Lys Gly305 310 315gca caa gtg aaa ggc aac gaa aca gtc aca gtc gac aaa ggg aaa gaa1491Ala Gln Val Lys Gly Asn Glu Thr Val Thr Val Asp Lys Gly Lys Glu320 325 330
act gaa gtc ggc atc gtc aca gaa aaa gcc ttt tca atg cct gtg aag1539Thr Glu Val Gly Ile Val Thr Glu Lys Ala Phe Ser Met Pro Val Lys335 340 345aac ggt gaa gaa aag aat tac aaa gcg aaa gtg acg ctg aac aaa gac1587Asn Gly Glu Glu Lys Asn Tyr Lys Ala Lys Val Thr Leu Asn Lys Asp350 355 360 365gag ctg acc gca cct gtt aaa aaa ggt gag aaa gtc ggt acc ttg acg1635Glu Leu Thr Ala Pro Val Lys Lys Gly Glu Lys Val Gly Thr Leu Thr370 375 380gcc tcc tat aag gga gaa gaa aag gac tac gga ttt ctc ggc agc gat1683Ala Ser Tyr Lys Gly Glu Glu Lys Asp Tyr GIy Phe Leu Gly Ser Asp385 390 395gta tca ggt gta aac ctg gtg aca aaa gaa gat gat gag aaa gca aac1731Val Ser Gly Val Asn Leu Val Thr Lys Glu Asp Asp Glu Lys Ala Ash400 405 410tgg ttt att tta acg atg aga agc atc ggc ggc ttt ttt gcc ggt ata1779Trp Phe Ile Leu Thr Met Arg Ser Ile Gly Gly Phe Phe Ala Gly Ile415 420 425tgg aac agc att gtt gaa atg gta acc ggc tgg ttt taa aaaataaaga 1828Trp Asn Ser Ile Val Glu Met Val Thr Gly Trp Phe430 435 440gctctatttg atagggctct ttattgtcat attcagatag aattaccatt tacaatccta 1888acgatttgtt attaaaatag aaataatctt cgtgtttttc atatgatttt gtggaattat 1948aggatacaat aggattaaat ctgacaattc aatctttgat tagggggatc cttgaaatgg 2008ctcaaacagg tactgatcgc gtaaaacgcg gtatggcaga aatgcagaag ggcggcgtca 2068ttatggacgt tgtcaatgct gaacaagcaa aaatcgcaga agaagcagga gccgtcgcag 2128tcatggcgct ggagcgcgtt ccggctgata tccgtgcggc aggcggagtt gcccgcatgg 2188cagatccgac tattgtcgaa gaagtgatga acgctgtttc aatcccggtt atggcaaaag 2248cacgcatcgg gcatatcgtt gaagcgcgtg ttcttgaagc gcttggcgtt gactatattg 2308atgaaa 2314<210>14<211>441<212>PRT<213>地衣芽孢桿菌<400>14Leu Lys Ser Lys Arg Leu Lys Gln Leu Ile Met Leu Ile Val Ala Phe1 5 10 15Ala Val Thr Val Gly Ala Phe Set Pro Met Ser Thr Ala Lys Ala Ala20 25 30Asn Asp Pro Ile Asn Val Asp Ala Lys Ala Ala Ile Leu Val Glu Ala35 40 45Ser Ser Gly Lys Ile Leu Tyr Ser Lys Asn Ala Asp Gln Arg Leu Pro50 55 60
Val Ala Ser Met Ala Lys Met Met Thr Glu Tyr Leu Leu Leu Glu Ala65 70 75 80Ile His Glu Gly Lys Val Lys Trp Asp Gln Lys Tyr Thr Pro Asp Asp85 90 95Tyr Val Tyr Glu Ile Ser Gln Asp Arg Ser Leu Ser Asn Val Pro Leu100 105 110Arg Lys Asp Gly Ser Tyr Thr Val Lys Glu Leu Tyr Gln Ala Thr Ala115 120 125Ile Tyr Ser Ala Asn Ala Ala Ala Ile Ala Leu Ser Glu Ile Val Ala130 135 140Gly Ser Glu Ser Lys Phe Val Glu Leu Met Asn Lys Lys Ala Lys Glu145 150 155 160Leu Gly Met Lys Asp Phe Lys Phe Val Asn Ala Thr Gly Leu Glu Asn165 170 175Lys Asp Leu His Gly Lys His Pro Ser Gly Thr Asn Ala Asn Glu Glu180 185 190Asn Glu Val Ser Ala Arg Asp Met Ala Leu Leu Ala Asp His Leu Val195 200 205Ser Asp Tyr Pro Glu Ile Leu Asp Thr Ala Ser Ile Ala Lys Thr Lys210 215 220Phe Arg Lys Gly Thr Asp Asp Glu Met Asp Met Pro Asn Trp Asn Phe225 230 235 240Met Leu Lys Gly Leu Val Gln Gln Tyr Glu Gly Val Asp Gly Leu Lys245 250 255Thr Gly Ser Thr Asp Ser Ala Gly Ser Cys Phe Thr Ala Thr Ala Glu260 265 270Arg Asn Gly Met Arg Val Ile Ser Val Val Leu Asn Ala Lys Gly Asp275 280 285Leu His Thr Ala Arg Phe Lys Glu Thr Lys Lys Met Leu Asp Tyr Ala290 295 300Phe Glu Asn Phe Thr Met Lys Glu Phe Tyr Ala Lys Gly Ala Gln Val305 310 315 320Lys Gly Asn Glu Thr Val Thr Val Asp Lys Gly Lys Glu Thr Glu Val325 330 335
Gly Ile Val Thr Glu Lys Ala Phe Ser Met Pro Val Lys Asn Gly Glu340 345 350Glu Lys Asn Tyr Lys Ala Lys Val Thr Leu Asn Lys Asp Glu Leu Thr355 360 365Ala Pro Val Lys Lys Gly Glu Lys Val Gly Thr Leu Thr Ala Ser Tyr370 375 380Lys Gly Glu Glu Lys Asp Tyr Gly Phe Leu Gly Ser Asp Val Ser Gly385 390 395 400Val Asn Leu Val Thr Lys Glu Asp Asp Glu Lys Ala Asn Trp Phe Ile405 410 415Leu Thr Met Arg Ser Ile Gly Gly Phe Phe Ala Gly Ile Trp Asn Ser420 425 430Ile Val Glu Met Val Thr Gly Trp Phe435 440<210>15<211>2431<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(519)..(1958)<400>15agaagcttga cgtcattgtt gccagcaatt tattcggcga tattttgacc gatatcgggg 60cggcgattat gggaagcata ggtgtggcgc cggccgccaa cattaatgta aacggcaaat 120atccatctat gtttgagcct gtgcacggct ctgctccgga catcatctga tagcgagtca 180tcatcggaaa aggaatcgcc aatccgatcg gccaaatttg gacggcaaaa atgatgctcg 240atcatttcgg ggaagaagaa ctcggtgcaa gattgcttga cgtgattgaa gacgttctaa 300agagcggcta cttaacgcct gatatcggcg gttcaagtac gacggatgat gtgacagaag 360aaatcatcaa gcgcgtgagg accatataag cgaaagctgc cagcactccg gcagcttttt 420ttgctgtgga atggaaaatc gattacatga tacgggcggt tttggcgcat actacatcaa 480gcaacaaaac atagtgcagg cagaagagga ggtatagg atg aat cag aag att gtg 536Met Asn Gln Lys Ile Val15tct gtg ctc atc att gcg ctc agc ctg tta tgc ggc ttg gta aca ggc584Ser Val Leu Ile Ile Ala Leu Ser Leu Leu Cys Gly Leu Val Thr Gly10 15 20gcc ggc ccg att ccg tta cat gat gtc gtt tca aaa gca tca gcg gct632Ala Gly Pro Ile Pro Leu His Asp Val Val Ser Lys Ala Ser Ala Ala25 30 35
gaa gaa gct gca gcc aaa agc acc gct aga aac gaa ttg gag aaa att 680Glu Glu Ala Ala Ala Lys Ser Thr Ala Arg Asn Glu Leu Glu Lys Ile40 45 50gag aaa gca tat gac ctg att tca aat gaa tat gtg gag cag gtc gac 728Glu Lys Ala Tyr Asp Leu Ile Ser Asn Glu Tyr Val Glu Gln Val Asp55 60 65 70agg gaa aaa ctg ttg gaa gga gcc ata cag ggc atg ctt tca aca ttg 776Arg Glu Lys Leu Leu Glu Gly Ala Ile Gln Gly Met Leu Ser Thr Leu75 80 85aat gac cca tac tcg gtc tat atg gat aaa cag acg gca aag cgg ttt 824Asn Asp Pro Tyr Ser Val Tyr Met Asp Lys Gln Thr Ala Lys Arg Phe90 95 100tca gat tcc ctt gat tcc tct ttt gaa gga atc ggt gca gaa atc gga 872Ser Asp Ser Leu Asp Ser Ser Phe Glu Gly Ile Gly Ala Glu Ile Gly105 110 115atg gaa gac aga aaa atc att atc gtt tct cct ttt aaa cag tct ccg 920Met Glu Asp Arg Lys Ile Ile Ile Val Ser Pro Phe Lys Gln Ser Pro120 125 130gca gag aaa gcc gga tta aag ccg aac gat gaa atc att agc atc gac 968Ala Glu Lys Ala Gly Leu Lys Pro Asn Asp Glu Ile Ile Ser Ile Asp135 140 145 150ggc gat tcc atg agc gga atg gat ttg aat gac gcc gta tta aag atc1016Gly Asp Ser Met Ser Gly Met Asp Leu Asn Asp Ala Val Leu Lys Ile155 160 165aga ggc aaa aag gga tcg acg gtt aca ctg aaa gtt cac cga ccc gga1064Arg Gly Lys Lys Gly Ser Thr Val Thr Leu Lys Val His Arg Pro Gly170 175 180atg aaa gac cag ctg aca ttt acg att aag cgc gat gaa atc ccg ctg1112Met Lys Asp Gln Leu Thr Phe Thr Ile Lys Arg Asp Glu Ile Pro Leu185 190 195gaa acg gtg ttt gct tcc ata aag aaa gta cag gat aaa cct gtc ggc1160Glu Thr Val Phe Ala Ser Ile Lys Lys Val Gln Asp Lys Pro Val Gly200 205 210tat atc gca ata tct tcc ttt tcc gaa cat acg gca aaa gat ttt act1208Tyr Ile Ala Ile Ser Ser Phe Ser Glu His Thr Ala Lys Asp Phe Thr215 220 225 230gct gaa ctg aaa aag ctt gag aaa aag gga ata aaa ggg ctc gtt ttg1256Ala Glu Leu Lys Lys Leu Glu Lys Lys Gly Ile Lys Gly Leu Val Leu235 240 245gat gta aga gga aat ccg ggc ggg tac ttg caa agc gtg gaa gac att1304Asp Val Arg Gly Asn Pro Gly Gly Tyr Leu Gln Ser Val Glu Asp Ile250 255 260tta aaa cat ttt gtg aca aaa gat cat ccg tat ata caa atc gcc gaa1352Leu Lys His Phe Val Thr Lys Asp His Pro Tyr Ile Gln Ile Ala Glu265 270 275aga aac gga aat aaa aaa caa tac ttc tca aaa tta aaa gag aaa aaa1400Arg Asn Gly Asn Lys Lys Gln Tyr Phe Ser Lys Leu Lys Glu Lys Lys280 285 290cca tac cct gtg agc gtg atc acg gac aag ggc agc gct tcg gct tcg1448Pro Tyr Pro Val Ser Val Ile Thr Asp Lys Gly Ser Ala Ser Ala Ser295 300 305 310
gaa att ctt gca ggc gca tta aaa gaa gcc gag ggc tac gac gtg gtt1496Glu Ile Leu Ala Gly Ala Leu Lys Glu Ala Glu Gly Tyr Asp Val Val315 320 325ggc gat cct tcc ttt gga aag gga acc gtc cag cag gcg gtg ccg atg1544Gly Asp Pro Ser Phe Gly Lys Gly Thr Val Gln Gln Ala Val Pro Met330 335 340gga gac ggt agc aat att aag ctg acg ctg tac aaa tgg ctg aca cct1592Gly Asp Gly Ser Asn Ile Lys Leu Thr Leu Tyr Lys Trp Leu Thr Pro345 350 355aaa gga aat tgg atc cat aag cag ggc att cag ccg acc gtt cca gtc1640Lys Gly Asn Trp Ile His Lys Gln Gly Ile Gln Pro Thr Val Pro Val360 365 370acg cag cct gca tat ttc tcg gcg ggg cct tta cag ctg aaa gaa ccc1688Thr Gln Pro Ala Tyr Phe Ser Ala Gly Pro Leu Gln Leu Lys Glu Pro375 380 385 390ctc aag ccg gat atg aac aac aat gaa att aag cgg gcg cag ttc ctg1736Leu Lys Pro Asp Met Asn Asn Asn Glu Ile Lys Arg Ala Gln Phe Leu395 400 405ctg aaa gga ctc ggg ttt gtc ccc ggc cgg gaa gac ggc tat tac aat1784Leu Lys Gly Leu Gly Phe Val Pro Gly Arg Glu Asp Gly Tyr Tyr Asn410 415 420gaa agc aca aaa aaa gcc gtc atg gcg ttt cag gca gca aac aag ctg1832Glu Ser Thr Lys Lys Ala Val Met Ala Phe Gln Ala Ala Asn Lys Leu425 430 435aag caa aca ggg atc att gac caa aaa acg gca aat aca atg aac ctg1880Lys Gln Thr Gly Ile Ile Asp Gln Lys Thr Ala Asn Thr Met Asn Leu440 445 450cgg att gag gag aaa aag atg gat gaa aag aac gat ctt cag ctg caa1928Arg Ile Glu Glu Lys Lys Met Asp Glu Lys Asn Asp Leu Gln Leu Gln455 460 465 470gcc gca tta aaa gtg ctg ttt aac aaa aag tgagggcacg ggatgtcctc 1978Ala Ala Leu Lys Val Leu Phe Asn Lys Lys475 480acttttttta ttctcagaaa gtaaattctc aatcttttta cctaaaattt ttgctttttc 2038ataaaaaaaa cattcataat gaatagaatt gacccgataa cataagtata tgggagactt 2098tataaggatg taaagatgca gtaaaaatgt aacttttgca agttgtataa cataggaaaa 2158ctttttaaaa tagaaccaaa gctttttcaa gaattataat gtgtgcattt tttttgcgga 2218gaatgttagc gctgtttgcg ttcttcgtct ctgttggtgg agtcgtctga cgcttgcgct 2278tttcttatat ataaataata aatgggggta cacgattgaa aagggcaagt attgtgagag 2338agaaaaaata ctatgaatta gtggagcagt taaaagttcg atcacaagac gttacgtttt 2398ccgctacaaa ggcagtagga ttgcttatgc tgt 2431<210>16<211>480<212>PRT<213>地衣芽孢桿菌<400>16
Met Asn Gln Lys Ile Val Ser Val Leu Ile Ile Ala Leu Ser Leu Leu1 5 10 15Cys Gly Leu Val Thr Gly Ala Gly Pro Ile Pro Leu His Asp Val Val20 25 30Ser Lys Ala Ser Ala Ala Glu Glu Ala Ala Ala Lys Ser Thr Ala Arg35 40 45Asn Glu Leu Glu Lys Ile Glu Lys Ala Tyr Asp Leu Ile Ser Asn Glu50 55 60Tyr Val Glu Gln Val Asp Arg Glu Lys Leu Leu Glu Gly Ala Ile Gln65 70 75 80Gly Met Leu Ser Thr Leu Asn Asp Pro Tyr Ser Val Tyr Met Asp Lys85 90 95Gln Thr Ala Lys Arg Phe Ser Asp Ser Leu Asp Ser Ser Phe Glu Gly100 105 110Ile Gly Ala Glu Ile Gly Met Glu Asp Arg Lys Ile Ile Ile Val Ser115 120 125Pro Phe Lys Gln Ser Pro Ala Glu Lys Ala Gly Leu Lys Pro Asn Asp130 135 140Glu Ile Ile Ser Ile Asp Gly Asp Ser Met Ser Gly Met Asp Leu Asn145 150 155 160Asp Ala Val Leu Lys Ile Arg Gly Lys Lys Gly Ser Thr Val Thr Leu165 170 175Lys Val His Arg Pro Gly Met Lys Asp Gln Leu Thr Phe Thr Ile Lys180 185 190Arg Asp Glu Ile Pro Leu Glu Thr Val Phe Ala Ser Ile Lys Lys Val195 200 205Gln Asp Lys Pro Val Gly Tyr Ile Ala Ile Ser Ser Phe Ser Glu His210 215 220Thr Ala Lys Asp Phe Thr Ala Glu Leu Lys Lys Leu Glu Lys Lys Gly225 230 235 240Ile Lys Gly Leu Val Leu Asp Val Arg Gly Asn Pro Gly Gly Tyr Leu245 250 255Gln Ser Val Glu Asp Ile Leu Lys His Phe Val Thr Lys Asp His Pro260 265 270
Tyr Ile Gln Ile Ala Glu Arg Asn Gly Asn Lys Lys Gln Tyr Phe Ser275 280 285Lys Leu Lys Glu Lys Lys Pro Tyr Pro Val Ser Val Ile Thr Asp Lys290 295 300Gly Ser Ala Ser Ala Ser Glu Ile Leu Ala Gly Ala Leu Lys Glu Ala305 310 315 320Glu Gly Tyr Asp Val Val Gly Asp Pro Ser Phe Gty Lys Gly Thr Val325 330 335Gln Gln Ala Val Pro Met Gly Asp Gly Ser Asn Ile Lys Leu Thr Leu340 345 350Tyr Lys Trp Leu Thr Pro Lys Gly Asn Trp Ile His Lys Gln Gly Ile355 360 365Gln Pro Thr Val Pro Val Thr Gln Pro Ala Tyr Phe Ser Ala Gly Pro370 375 380Leu Gln Leu Lys Glu Pro Leu Lys Pro Asp Met Asn Asn Asn Glu Ile385 390 395 400Lys Arg Ala Gln Phe Leu Leu Lys Gly Leu Gly Phe Val Pro Gly Arg405 410 415Glu Asp Gly Tyr Tyr Asn Glu Ser Thr Lys Lys Ala Val Met Ala Phe420 425 430Gln Ala Ala Asn Lys Leu Lys Gln Thr Gly Ile Ile Asp Gln Lys Thr435 440 445Ala Asn Thr Met Asn Leu Arg Ile Glu Glu Lys Lys Met Asp Glu Lys450 455 460Asn Asp Leu Gln Leu Gln Ala Ala Leu Lys Val Leu Phe Asn Lys Lys465 470 475 480<210>17<211>2230<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1730)<400>17ctgtcctccg gagctgagcg gagacattgt cgaccggggc gtgattttga ccggaggagg 60cgcgcttttg aacggcatca aagaatggct gtcacaggaa atcgttgtgc ccgtgcatgt120tgctgcaaat ccgcttgaat ctgtcgctat cggaacgggt cgttctctcg aagtgatcga180
taaattgcaa aaagcggcta aataattttt gttttcgcgt cttttttccc ggcttctttc 240atatcgtgag gaatggagaa acgataagga ggaagaccat gcttagagat atgggaagac 300gcctggccat caccgttatt ttaagcggta ttattctcgg ggggatgagc atctcccttg 360caaacatgcc aagctcgccc cccggccaaa cggccaaaat caaccgttaa aaaagaagca 420gcctgaaccg ggctgcttct ttttttcttt tcaatcaaat atttgatatg atgttatgga 480caataaagga ggttttgcca atg aat tcg ttt act gat aaa ctg agc aaa tat 533Met Asn Ser Phe Thr Asp Lys Leu Ser Lys Tyr1 5 10gca aag ctt gtg gcc gaa gtc ggc gta aac gtt caa aaa gga cag caa 581Ala Lys Leu Val Ala Glu Val Gly Val Asn Val Gln Lys Gly Gln Gln15 20 25gtc gtc atc aac gcg tca aca gaa gta cgc gat ttt gca cgg ctc ctc 629Val Val Ile Asn Ala Ser Thr Glu Val Arg Asp Phe Ala Arg Leu Leu30 35 40gtc aaa agc gcc tat caa aga ggg gcg aaa aac gtg acg gtc cgc tgg 677Val Lys Ser Ala Tyr Gln Arg Gly Ala Lys Asn Val Thr Val Arg Trp45 50 55cag gat gat gag gtc aca agg ctg aaa tac gag ctg gct cct gcg gaa 725Gln Asp Asp Glu Val Thr Arg Leu Lys Tyr Glu Leu Ala Pro Ala Glu60 65 70 75gcc ttt gaa gag ttc cct gaa tgg gaa gca aag ggg ctt gag acg ctc 773Ala Phe Glu Glu Phe Pro Glu Trp Glu Ala Lys Gly Leu Glu Thr Leu80 85 90gcc aaa gaa ggc gct gct ttt att tcc att gtc tct tca agt cct gat 821Ala Lys Glu Gly Ala Ala Phe Ile Ser Ile Val Ser Ser Ser Pro Asp95 100 105ctt ttg aaa ggc atc gat tcc aaa aaa atc tcc gca cag cag aaa gcc 869Leu Leu Lys Gly Ile Asp Ser Lys Lys Ile Ser Ala Gln Gln Lys Ala110 115 120gca gga aaa gcg atg cat aca tac agg caa atg att caa tcg gat aaa 917Ala Gly Lys Ala Met His Thr Tyr Arg Gln Met Ile Gln Ser Asp Lys125 130 135gtc agc tgg act gtc gtc gca gcg cct tcg ccg gcc tgg gcg aaa aaa 965Val Ser Trp Thr Val Val Ala Ala Pro Ser Pro Ala Trp Ala Lys Lys140 145 150 155gta ttc cct gag gca gat gaa gag aca gcc gtg caa aag cta tgg aat1013Val Phe Pro Glu Ala Asp Glu Glu Thr Ala Val Gln Lys Leu Trp Asn160 165 170gag att ttc aaa acg acc cgc gtt gac cgt gaa gat ccc gtt caa gcg1061Glu Ile Phe Lys Thr Thr Arg Val Asp Arg Glu Asp Pro Val Gln Ala175 180 185tgg aaa gat cac gat caa acc ttg cgc gat aaa gta agc gtc tta aac1109Trp Lys Asp His Asp Gln Thr Leu Arg Asp Lys Val Ser Val Leu Asn190 195 200gac aag cat tat cac gcc ctt cat tac caa gca aag gga aca gat ttg1157Asp Lys His Tyr His Ala Leu His Tyr Gln Ala Lys Gly Thr Asp Leu205 210 215acg atc gag ctt ccc gag aag cat ctg tgg gtc ggc gcc gga agc aca1205
Thr Ile Glu Leu Pro Glu Lys His Leu Trp Val Gly Ala Gly Ser Thr220 225 230 235aac gaa aac ggc gtc gag ttc atg gcc aat atg ccg aca gag gaa gta 1253Asn Glu Asn Gly Val Glu Phe Met Ala Asn Met Pro Thr Glu Glu Val240 245 250ttc act gct ccg aaa aaa gac ggg gtg aac ggc acc gtt tca agc acg 1301Phe Thr Ala Pro Lys Lys Asp Gly Val Asn Gly Thr Val Ser Ser Thr255 260 265aag ccg tta agc tat ggc ggc aat tta atc gac ggc ttt aca ctg aca 1349Lys Pro Leu Ser Tyr Gly Gly Asn Leu Ile Asp Gly Phe Thr Leu Thr270 275 280ttt aaa aac gga cgg att acg gat gtc aaa gct gga caa ggc gaa gaa 1397Phe Lys Asn Gly Arg Ile Thr Asp Val Lys Ala Gly Gln Gly Glu Glu285 290 295att tta aaa gag ctg atc gaa acg gat gag ggt tca cac tac tta ggc 1445Ile Leu Lys Glu Leu Ile Glu Thr Asp Glu Gly Ser His Tyr Leu Gly300 305 310 315gaa att gcg ctt gtt gcc cac gat tcg ccg att tcc aag tcc aat att 1493Glu Ile Ala Leu Val Ala His Asp Ser Pro Ile Ser Lys Ser Asn Ile320 325 330ctg ttt tac aac aca ctg ttt gat gaa aat gcc tca aac cac ctc gcg 1541Leu Phe Tyr Asn Thr Leu Phe Asp Glu Asn Ala Ser Asn His Leu Ala335 340 345atc ggc agt gca tac gca ttc aac atc gaa ggc gga aaa cag atg tcg 1589Ile Gly Ser Ala Tyr Ala Phe Asn Ile Glu Gly Gly Lys Gln Met Ser350 355 360cgc gag gag ctg gcg aaa gaa gga tta aac gaa agc atc aca cat gtt 1637Arg Glu Glu Leu Ala Lys Glu Gly Leu Asn Glu Ser Ile Thr His Val365 370 375gac ttt atg atc ggt tca gga gaa atg aat ata gac gga atc act gct 1685Asp Phe Met Ile Gly Ser Gly Glu Met Asn Ile Asp Gly Ile Thr Ala380 385 390 395gac gga aaa aga gag ccg ata ttc aga aac ggc aac tgg gcg ata 1730Asp Gly Lys Arg Glu Pro Ile Phe Arg Asn Gly Asn Trp Ala Ile400 405 410taaagccttc cgcaaaaaaa gacctcagaa aacttctgag gtccttttat atcgtgtccg1790catgccgccc gcaatcctaa acttccctgt acatgcatgg cggctgtaac agctgaaaat1850caggtccatg ctccgccgcc tcatcgcttg ggcaaactgc ttaaatgttc atgcttcagt1910ttccagcgtc ctgactcgtc ctttacaaat acgtttgtcg cacggcctgc cccttgaacc1970ggttcgcccc tatataaccc tttccattcg tatgtataaa gacagacggc ctgttcgtcc2030gtttccgtga tccagctgac atgaccggcg ctgtatactt cattttcgat ttgcttccag2090gcgttttcaa aataacgctg aatctcctca aagcccgagc acgtttgatc cgaaaaccag2150tacacagcgc cggggtcaag gagggctttg acattctcaa actcatgtgt gtttgttgct2210tgaatatatt gctcaagagc2230<210>18<211>410
<212>PRT<213>地衣芽孢桿菌<400>18Met Asn Ser Phe Thr Asp Lys Leu Ser Lys Tyr Ala Lys Leu Val Ala1 5 10 15Glu Val Gly Val Asn Val Gln Lys Gly Gln Gln Val Val Ile Asn Ala20 25 30Ser Thr Glu Val Arg Asp Phe Ala Arg Leu Leu Val Lys Ser Ala Tyr35 40 45Gln Arg Gly Ala Lys Asn Val Thr Val Arg Trp Gln Asp Asp Glu Val50 55 60Thr Arg Leu Lys Tyr Glu Leu Ala Pro Ala Glu Ala Phe Glu Glu Phe65 70 75 80Pro Glu Trp Glu Ala Lys Gly Leu Glu Thr Leu Ala Lys Glu Gly Ala85 90 95Ala Phe Ile Ser Ile Val Ser Ser Ser Pro Asp Leu Leu Lys Gly Ile100 105 110Asp Ser Lys Lys Ile Ser Ala Gln Gln Lys Ala Ala Gly Lys Ala Met115 120 125His Thr Tyr Arg Gln Met Ile Gln Ser Asp Lys Val Ser Trp Thr Val130 135 140Val Ala Ala Pro Ser Pro Ala Trp Ala Lys Lys Val Phe Pro Glu Ala145 150 155 160Asp Glu Glu Thr Ala Val Gln Lys Leu Trp Asn Glu Ile Phe Lys Thr165 170 175Thr Arg Val Asp Arg Glu Asp Pro Val Gln Ala Trp Lys Asp His Asp180 185 190Gln Thr Leu Arg Asp Lys Val Ser Val Leu Asn Asp Lys His Tyr His195 200 205Ala Leu His Tyr Gln Ala Lys Gly Thr Asp Leu Thr Ile Glu Leu Pro210 215 220Glu Lys His Leu Trp Val Gly Ala Gly Ser Thr Asn Glu Asn Gly Val225 230 235 240Glu Phe Met Ala Asn Met Pro Thr Glu Glu Val Phe Thr Ala Pro Lys245 250 255
Lys Asp Gly Val Asn Gly Thr Val Ser Ser Thr Lys Pro Leu Ser Tyr260 265 270Gly Gly Asn Leu Ile Asp Gly Phe Thr Leu Thr Phe Lys Asn Gly Arg275 280 285Ile Thr Asp Val Lys Ala Gly Gln Gly Glu Glu Ile Leu Lys Glu Leu290 295 300Ile Glu Thr Asp Glu Gly Ser His Tyr Leu Gly Glu Ile Ala Leu Val305 310 315 320Ala His Asp Ser Pro Ile Ser Lys Ser Asn Ile Leu Phe Tyr Asn Thr325 330 335Leu Phe Asp Glu Asn Ala Ser Asn His Leu Ala Ile Gly Ser Ala Tyr340 345 350Ala Phe Asn Ile Glu Gly Gly Lys Gln Met Ser Arg Glu Glu Leu Ala355 360 365Lys Glu Gly Leu Asn Glu Ser Ile Thr His Val Asp Phe Met Ile Gly370 375 380Ser Gly Glu Met Asn Ile Asp Gly Ile Thr Ala Asp Gly Lys Arg Glu385 390 395 400Pro Ile Phe Arg Asn Gly Asn Trp Ala Ile405 410<210>19<211>2390<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(1273)..(2388)<400>19caggtgaaaa agatataaaa atgaaagggc cgatatcaat cggccttttc atattaccaa 60aaaaacaagc ctgcaagagc cacaccggca attagaccga ggccaagtcc gtatacaggg 120ccccaacccc accagccgcc gtaatatccg taggcgaacc cgcggcctcc ccgtctgatc 180atcggggcaa tatatacttt gcttggtgaa acacggacga ttcttccgac atggacgcgg 240cctcctttgt cggtgattct cgcaactctg ccaatatttt ggtggcaaaa cctataatat 300cggtccactt gaatgctcct tccatcatct aatctattcc tagtcagctt atgacggatt 360taatagaaaa ggttggacga aagaaggagg acaaatgatg atttatagat gtgatataaa 420aataatatga tcgagagagt cccggtattt ttccaggtaa tgcaggtgtt tttccatcgt 480ttcatagtct ttgtcggaaa ttgcaggcga cagatccatt tcaacggcgt catccgggtc 540
tcctgcatct tccaatacga caggaatgat gtcaatcttt gaaaatcctt cttctcgaag 600gacattgacc cactcatcct cgtcatataa tgcggtgaat ccgtaaaatt ccgtgatttc 660tttttttgct tcgtctgtca gtgcgtttcc ttccttaagg cagacttcga tcgcgaccag 720catccctccg ggttttaaaa tccgccggat ttcctgtaaa gcagaccgga tatttgtgaa 780gctcagcaca gattcagtta atacacaggc aaagctttca gactgaaacg gcatttcctc 840aagtggagag cggacagccg gaatggaaag ctgttctttt ttaaaacgct gtcgggcttt 900atcgatcatg accgggtcgg catcaagggc agtgacagga aacagaagct gacccaaata 960agcggcggtc tggccggttc cgcagcctgc atcaagaatc ggctgctcag ggtcgatgcc 1020ggctttggcg agcactgcct ttgacaggga catcccgccg ggatgagcgg atgagacgcc 1080gaagctggcg agcatgtcta aataatcgct caaattgaga tccctccttt tttgtatgca 1140tatgcaaaag cggaataatg gttgaaaacg atcgaatgcc agcatcccgg aaaaatcggt 1200atttaagtga ttcgagttga cgaataaaga ttcgtccatg ataatatggt aagaagaaaa 1260ggtggtgttc gt ttg aaa cag cag ttg aaa ctc ttt ttg gct gta ttg att 1311Leu Lys Gln Gln Leu Lys Leu Phe Leu Ala Val Leu Ilel 5 10acg gcc gtc gcc gcc tcc gca ctg acg ctt tat ctg gtg ggc aaa ggc1359Thr Ala Val Ala Ala Ser Ala Leu Thr Leu Tyr Leu Val Gly Lys Gly15 20 25agc ggc ggt tca gta tat tcc gct tca gat tcc gag aaa ttc gaa aag1407Ser Gly Gly Ser Val Tyr Ser Ala Ser Asp Ser Glu Lys Phe Glu Lys30 35 40 45ctg atg gcg gcg tac gac aaa atc aag agc gac tat tat caa aat gca1455Leu Met Ala Ala Tyr Asp Lys Ile Lys Ser Asp Tyr Tyr Gln Asn Ala50 55 60gac gat gaa aag ctg act gac ggc gcg att aaa ggg atg ctt gga gct1503Asp Asp Glu Lys Leu Thr Asp Gly Ala Ile Lys Gly Met Leu Gly Ala65 70 75ttg gat gat cca tat tct aca tac atg gat aag aaa gaa gct aaa agc1551Leu Asp Asp Pro Tyr Ser Thr Tyr Met Asp Lys Lys Glu Ala Lys Ser80 85 90ttt gaa gaa agc att aca tct tct ttt gaa gga atc ggc gcc caa gta1599Phe Glu Glu Ser Ile Thr Ser Ser Phe Glu Gly Ile Gly Ala Gln Val95 100 105gaa gaa aaa aat gga caa att cta atc gtc gcg cct att aaa gga tct1647Glu Glu Lys Asn Gly Gln Ile Leu Ile Val Ala Pro Ile Lys Gly Ser110 115120 125ccc gca gaa aaa gcc ggc tta aag ccg cat gat gaa att cag aaa gtg1695Pro Ala Glu Lys Ala Gly Leu Lys Pro His Asp Glu Ile Gln Lys Val130 135 140gac ggc aaa agc gta aaa ggc aag acc gtc aac gaa gcg aca gcg atg1743Asp Gly Lys Ser Val Lys Gly Lys Thr Val Asn Glu Ala Thr Ala Met145 150 155atc cgc ggc aaa aaa ggc acg aat gtc aaa ctt gtt ctc aac agg gaa1791Ile Arg Gly Lys Lys Gly Thr Asn Val Lys Leu Val Leu Asn Arg Glu160 165 170ggc gtc ggc caa atc gat gtt acg att aag cgc gat acg att ccg atc1839
Gly Val Gly Gln Ile Asp Val Thr Ile Lys Arg Asp Thr Ile Pro Ile175 180 185gaa acg gtt tat tcc aaa atg atc gac ggc aat atc ggt gaa atc caa1887Glu Thr Val Tyr Ser Lys Met Ile Asp Gly Asn Ile Gly Glu Ile Gln190 195 200 205att acg tct ttc tct gaa aat acc gca aaa gaa ctg aca aaa gcc att1935Ile Thr Ser Phe Ser Glu Asn Thr Ala Lys Glu Leu Thr Lys Ala Ile210 215 220gat gac ttg tct gaa aaa ggt gcg gaa cga ttc gtg ctg gat ctc aga1983Asp Asp Leu Ser Glu Lys Gly Ala Glu Arg Phe Val Leu Asp Leu Arg225 230 235gga aat cca ggc ggg ctg atg gac caa gcg att atg atg agc aac atg2031Gly Asn Pro Gly Gly Leu Met Asp Gln Ala Ile Met Met Ser Asn Met240 245 250ttt gtc gat aaa ggc aaa acc atc atg cag gtt gaa tcc aaa aac gga2079Phe Val Asp Lys Gly Lys Thr Ile Met Gln Val Glu Ser Lys Asn Gly255 260 265aaa aaa gaa gtg tac aaa gct gag aag gag cga aaa gtg aac aag ccg2127Lys Lys Glu Val Tyr Lys Ala Glu Lys Glu Arg Lys Val Asn Lys Pro270 275 280 285acc gtt gtt ctc gtc aac gga gga acg gca agc gcc gct gag atc atg2175Thr Val Val Leu Val Asn Gly Gly Thr Ala Ser Ala Ala Glu Ile Met290 295 300gcc gca gca ctt cac cag tct tca ggc atc ccg atc gtc ggt gaa aat2223Ala Ala Ala Leu His Gln Ser Ser Gly Ile Pro Ile Val Gly Glu Asn305 310 315acg ttt ggg aaa ggg acg gtt caa aat gcg gag agc ttc tcc gac gga2271Thr Phe Gly Lys Gly Thr Val Gln Asn Ala Glu Ser Phe Ser Asp Gly320 325 330tcg acc gtc aag ctg acg att gcc aag tgg ctg acg ccg aat ggg gac2319Ser Thr Val Lys Leu Thr Ile Ala Lys Trp Leu Thr Pro Asn Gly Asp335 340 345tgg att cac gaa aaa gga atc aaa cct caa tat aaa gcg gag ctc cca2367Trp Ile His Glu Lys Gly Ile Lys Pro Gln Tyr Lys Ala Glu Leu Pro350 355 360 365agc tat gcc aaa ctg ccg tac ct 2390Ser Tyr Ala Lys Leu Pro Tyr370<210>20<211>372<212>PRT<213>地衣芽孢桿菌<400>20Leu Lys Gln Gln Leu Lys Leu Phe Leu Ala Val Leu Ile Thr Ala Val1 5 10 15Ala Ala Ser Ala Leu Thr Leu Tyr Leu Val Gly Lys Gly Ser Gly Gly20 25 30Ser Val Tyr Ser Ala Ser Asp Ser Glu Lys Phe Glu Lys Leu Met Ala
35 40 45Ala Tyr Asp Lys Ile Lys Ser Asp Tyr Tyr Gln Asn Ala Asp Asp Glu50 55 60Lys Leu Thr Asp Gly Ala Ile Lys Gly Met Leu Gly Ala Leu Asp Asp65 70 75 80Pro Tyr Ser Thr Tyr Met Asp Lys Lys Glu Ala Lys Ser Phe Glu Glu85 90 95Ser Ile Thr Ser Ser Phe Glu Gly Ile Gly Ala Gln Val Glu Glu Lys100 105 110Asn Gly Gln Ile Leu Ile Val Ala Pro Ile Lys Gly Ser Pro Ala Glu115 120 125Lys Ala Gly Leu Lys Pro His Asp Glu Ile Gln Lys Val Asp Gly Lys130 135 140Ser Val Lys Gly Lys Thr Val Asn Glu Ala Thr Ala Met Ile Arg Gly145 150 155 160Lys Lys Gly Thr Asn Val Lys Leu Val Leu Asn Arg Glu Gly Val Gly165 170 175Gln Ile Asp Val Thr Ile Lys Arg Asp Thr Ile Pro Ile Glu Thr Val180 185 190Tyr Ser Lys Met Ile Asp Gly Asn Ile Gly Glu Ile Gln Ile Thr Ser195 200 205Phe Ser Glu Asn Thr Ala Lys Glu Leu Thr Lys Ala Ile Asp Asp Leu210 215 220Ser Glu Lys Gly Ala Glu Arg Phe Val Leu Asp Leu Arg Gly Asn Pro225 230 235 240Gly Gly Leu Met Asp Gln Ala Ile Met Met Ser Asn Met Phe Val Asp245 250 255Lys Gly Lys Thr Ile Met Gln Val Glu Ser Lys Asn Gly Lys Lys Glu260 265 270Val Tyr Lys Ala Glu Lys Glu Arg Lys Val Asn Lys Pro Thr Val Val275 280 285Leu Val Asn Gly Gly Thr Ala Ser Ala Ala Glu Ile Met Ala Ala Ala290 295 300Leu His Gln Ser Ser Gly Ile Pro Ile Val Gly Glu Asn Thr Phe Gly
305 310 315 320Lys Gly Thr Val Gln Asn Ala Glu Ser Phe Ser Asp Gly Ser Thr Val325 330 335Lys Leu Thr Ile Ala Lys Trp Leu Thr Pro Asn Gly Asp Trp Ile His340 345 350Glu Lys Gly Ile Lys Pro Gln Tyr Lys Ala Glu Leu Pro Ser Tyr Ala355 360 365Lys Leu Pro Tyr370<210>21<211>2772<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1406)<400>21ttcttgaatc tgcggatata ttggcgtaag tattgtacga taaatccaaa tagcgaccca60ccctaaacac aatgcaataa ttatcttttt gtaataattg gggtcatgtt taattgtttt 120ttccacggta tgagaacgct cccttctttt aatctatttt ttatttaatt aaattgtagg 180ttgtaagcca gtcaataaca ttgttcactt tcaacaaaaa caaaagtaac attatacatt 240ttagacaaaa ggcgtcgctt aaaaatgatt catcgataga agctggcctt atgactcttt 300tttaaaacta acttggagta cccaatttaa cttcccgaat aaacaggaag cttcacaaat 360gttgatataa cagtgttttt aacgttttaa aaactaaagg tgcatgacaa gcgaccttaa 420gataagcttt ctttctcttt gaaaattcaa aaatcctccg atatatatag cgaacgccgc 480taaaaaagga ggagagccgg atg aaa aag tta tgg aaa atc gcg gtt tcg gct 533Met Lys Lys Leu Trp Lys Ile Ala Val Ser Ala1 5 10gca atg ttc gtc ggt ttt ttc gca aat tca ccc cgc att caa gcg gaa 581Ala Met Phe Val Gly Phe Phe Ala Asn Ser Pro Arg Ile Gln Ala Glu15 20 25agc aat aag cag gaa aac gag gtc att gtt gta tat aaa aat acc agc 629Ser Asn Lys Gln Glu Asn Glu Val Ile Val Val Tyr Lys Asn Thr Ser30 35 40gga aaa gaa acc gtc atc gaa cag gca gac acg gta gaa cac gtt tac 677Gly Lys Glu Thr Val Ile Glu Gln Ala Asp Thr Val Glu His Val Tyr45 50 55cgg cac att ccc gca gcc gct gtc act gcg gac gac aaa aca gtg cgc 725Arg His Ile Pro Ala Ala Ala Val Thr Ala Asp Asp Lys Thr Val Arg60 65 70 75gaa ctt gaa cac gat ccc gac gtc ctg tat gtc gaa gac aac ctc ccg 773Glu Leu Glu His Asp Pro Asp Val Leu Tyr Val Glu Asp Asn Leu Pro
80 85 90gta gct gct gcc gac agc acc gct cta aaa gct ttc tcc agc agc aca 821Val Ala Ala Ala Asp Ser Thr Ala Leu Lys Ala Phe Ser Ser Ser Thr95 100 105gcg caa aac gcc tcc gcg ttt tca cag tgg aac atc aag ctg att caa 869Ala Gln Asn Ala Ser Ala Phe Ser Gln Trp Asn Ile Lys Leu Ile Gln110 115 120gcc gca ctg gct tgg aat aaa ggt ttg acc gga aaa cag gtg aag atc 917Ala Ala Leu Ala Trp Asn Lys Gly Leu Thr Gly Lys Gln Val Lys Ile125 130 135gcg gtt att gac agc ggg att tcc ccc cat gag gag ctg tcg atc gcc 965Ala Val Ile Asp Ser Gly Ile Ser Pro His Glu Glu Leu Ser Ile Ala140 145 150 155ggc ggt gca tcg atg gtc ggc tat acc gct tca tac cgt gac gat aat1013Gly Gly Ala Ser Met Val Gly Tyr Thr Ala Ser Tyr Arg Asp Asp Asn160 165 170ggc cac gga acc cat gtt gcc gga atc atc gga gcg aag cat aac gga1061Gly His Gly Thr His Val Ala Gly Ile Ile Gly Ala Lys His Asn Gly175 180 185cgg ggg atc gac ggc atc gcg ccc ggc gcg cag ctg tat gcc gta aaa1109Arg Gly Ile Asp Gly Ile Ala Pro Gly Ala Gln Leu Tyr Ala Val Lys190 195 200gcg ctg gac cgg aat ggt gcg ggg gat ctg aaa ggt atc tta caa ggc1157Ala Leu Asp Arg Asn Gly Ala Gly Asp Leu Lys Gly Ile Leu Gln Gly205 210 215atc gat tgg tcg atc caa cac gga atc gat atc atc aat atg agc ctc1205Ile Asp Trp Ser Ile Gln His Gly Ile Asp Ile Ile Asn Met Ser Leu220 225 230 235gtc gtt tca ggc gac agt caa gtt ctc cac gat gcg gta gat aaa gca1253Val Val Ser Gly Asp Ser Gln Val Leu His Asp Ala Val Asp Lys Ala240 245 250tac aaa cgg gga atc att tta gtc gga gcg agc gga aat gcc gga aac1301Tyr Lys Arg Gly Ile Ile Leu Val Gly Ala Ser Gly Asn Ala Gly Asn255 260 265gga aaa tcc gtt tat tac ccg gcc gcc tac agc agc gtc att gcc gtc1349Gly Lys Ser Val Tyr Tyr Pro Ala Ala Tyr Ser Ser Val Ile Ala Val270 275 280tcg gca acc aat gaa aaa aat cag atc gcc tca ttt acc aac acc cgg1397Ser Ala Thr Asn Glu Lys Asn Gln Ile Ala Ser Phe Thr Asn Thr Arg285 290 295agc gcc gta tagtattccg ctccgagcac atctattatc aatacatcgg1446Ser Ala Val300gcaatcgcgg atatgcaatc ggaagctgca ctgcacaagg gacacccgac gtcacctgcg 1506tgatcgcact tatgaaacag ctgcacccaa ccgcttcaaa tgctgagctg cgcaagaaaa 1566tgcagtttta tacaagcgat ttgggcgctc ccggtcgtga tcatttgttc ggctaccgat 1626taatccgctt caaagaggtc acacagccat tggaaaaagc gcaaaaagcc gtcggacaag 1686cggaaaagac aaagaaaaaa gcggacattc aaaccgcaca aaaagcgatc gaaccgctcc 1746ctgcagacgc agataaaccc gctttgaaaa agagactcaa tactgttaaa gagcagctta 1806
aaaaaacagc cgaaagcaaa gtgaaactcg ctgagaagca aaaaaagaaa accaatgccg1866actccgccca aaaagcggtc aacgaactcg acagcggcac tttcaaaacg aatttgcaaa1926aacggatcaa cgcggtccgt tcaagcctct tgaagaccgc aaagcaggcc gttgccaaag1986ctgaaaaagc ggcaacagac tcaaacctcg gcaaagcgca aaaagcgatc aatgagcttc2046ccgccggaaa agacaaatca aatctgcaaa aaaggctgaa caccgcgaaa aagcaagcag2106ctgcagccta taataaaaaa gtttctgcag caaaggccaa agtcaaaacg gccgaacaaa2166agagaacgaa aaaaacaaaa tcagcagcgc aatcagcagt aggcaagctg aaagcatccg2226ccgaaaaaac gaagctgcaa aaacggatca atgccattaa gctgaagtag tacggaaaaa2286agcccggaat catccgggcc ttttaatttt catatttagc aaatgcgtca ttattgatgc2346tgttgtacta attgacattt cgggctcgtc atttgcgtaa tcgtattcct tggtcggccg2406cttactctcc cccgtactct ccaatcactt cttatcaatt ctttccgtca caatcccgac2466gatgtcccgt tctgaaacaa agccgaaatg gcggctgtca aagctgtgga tccggttgtc2526cccgaggacg aagtactcgt tttcaggcac cttctcctct ccggtcactt cctgcagtgt2586aaaatcccct gtcaagtccc ctccggcggt gacggccttt aagtcgtcca gataaggctc2646tttgatcttt tcttcgttga tatacagctg atcatcttca tacctgaggg tttcgcccgg2706aagcccgatc acccttttaa tgaatatatc tttgtcaggt cctttaaaca gcacgatgtc2766aaatcg 2772<210>22<211>302<212>PRT<213>地衣芽孢桿菌<400>22Met Lys Lys Leu Trp Lys Ile Ala Val Ser Ala Ala Met Phe Val Gly1 5 10 15Phe Phe Ala Asn Ser Pro Arg Ile Gln Ala Glu Ser Asn Lys Gln Glu20 25 30Asn Glu Val Ile Val Val Tyr Lys Asn Thr Ser Gly Lys Glu Thr Val35 40 45Ile Glu Gln Ala Asp Thr Val Glu His Val Tyr Arg His Ile Pro Ala50 55 60Ala Ala Val Thr Ala Asp Asp Lys Thr Val Arg Glu Leu Glu His Asp65 70 75 80Pro Asp Val Leu Tyr Val Glu Asp Asn Leu Pro Val Ala Ala Ala Asp85 90 95Ser Thr Ala Leu Lys Ala Phe Ser Ser Ser Thr Ala Gln Asn Ala Ser100 105 110
Ala Phe Ser Gln Trp Asn Ile Lys Leu Ile Gln Ala Ala Leu Ala Trp115 120 125Asn Lys Gly Leu Thr Gly Lys Gln Val Lys Ile Ala Val Ile Asp Ser130 135 140Gly Ile Ser Pro His Glu Glu Leu Ser Ile Ala Gly Gly Ala Ser Met145 150 155 160Val Gly Tyr Thr Ala Ser Tyr Arg Asp Asp Asn Gly His Gly Thr His165 170 175Val Ala Gly Ile Ile Gly Ala Lys His Asn Gly Arg Gly Ile Asp Gly180 185 190Ile Ala Pro Gly Ala Gln Leu Tyr Ala Val Lys Ala Leu Asp Arg Asn195 200 205Gly Ala Gly Asp Leu Lys Gly Ile Leu Gln Gly Ile Asp Trp Ser Ile210 215 220Gln His Gly Ile Asp Ile Ile Asn Met Ser Leu Val Val Ser Gly Asp225 230 235 240Ser Gln Val Leu His Asp Ala Val Asp Lys Ala Tyr Lys Arg Gly Ile245 250 255Ile Leu Val Gly Ala Ser Gly Asn Ala Gly Asn Gly Lys Ser Val Tyr260 265 270Tyr Pro Ala Ala Tyr Ser Ser Val Ile Ala Val Ser Ala Thr Asn Glu275 280 285Lys Asn Gln Ile Ala Ser Phe Thr Asn Thr Arg Ser Ala Val290 295 300<210>23<211>2185<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(214)..(1149)<400>23atcagggcat gcagacggac cggtttattt tcaacctcgg acacgggatc tttccagaag 60tgagtcccga cacactgaaa aaattgacgg catttgttca tgagtattcc gcctcaaaaa120aatcgagcct gatcacatga gcatttcagt tggtcttttc aatataaact gtcaaaatac180gtatagatca tgatctttaa agaggtgttc acg ttg ggt aaa aag aaa atg ggg 234Leu Gly Lys Lys Lys Met Gly
ctt ctt gtc atg gca tat ggg acg ccg tac aaa gag gaa gac att gaa 282Leu Leu Val Met Ala Tyr Gly Thr Pro Tyr Lys Glu Glu Asp Ile Glu10 15 20cgt tat tat aca cat atc aga aga ggc aga aag cct gag cct gac atg 330Arg Tyr Tyr Thr His Ile Arg Arg Gly Arg Lys Pro Glu Pro Asp Met25 30 35ctt cag gac tta aaa gac cgc tat aag gcg atc gga gga att tct ccg 378Leu Gln Asp Leu Lys Asp Arg Tyr Lys Ala Ile Gly Gly Ile Ser Pro40 45 50 55ctt gca aag atc acg cag gag cag acg aag cag ctg gaa aaa cgc tta 426Leu Ala Lys Ile Thr Gln Glu Gln Thr Lys Gln Leu Glu Lys Arg Leu60 65 70aac gag ctt cag gat gat gtg gtg ttc aag gca tat atc ggt ctc aaa 474Asn Glu Leu Gln Asp Asp Val Val Phe Lys Ala Tyr Ile Gly Leu Lys75 80 85cat atc gaa cct ttc att gag gat gcc gtt cgg gag atg cat aaa gac 522His Ile Glu Pro Phe Ile Glu Asp Ala Val Arg Glu Met His Lys Asp90 95 100ggg att aca gaa gcc gtc agc atc gtg ctc gcg ccc cat ttc tcc acc 570Gly Ile Thr Glu Ala Val Ser Ile Val Leu Ala Pro His Phe Ser Thr105 110 115ttt agc gta cag tcg tac aac aag cgg gcg aaa gac gag gct gaa aag 618Phe Ser Val Gln Ser Tyr Asn Lys Arg Ala Lys Asp Glu Ala Glu Lys120 125 130 135ctc ggc gaa ttg tcg atc aca tcg atc aac agc tgg tat gat gag ccg 666Leu Gly Glu Leu Ser Ile Thr Ser Ile Asn Ser Trp Tyr Asp Glu Pro140 145 150aaa ttt atc gct tat tgg gcg gat cag gtg aga aaa atc tat gac gaa 714Lys Phe Ile Ala Tyr Trp Ala Asp Gln Val Arg Lys Ile Tyr Asp Glu155 160 165atg cct tcg gaa gag agg gaa aac gcc gtc ttg att gta tcg gcg cac 762Met Pro Ser Glu Glu Arg Glu Asn Ala Val Leu Ile Val Ser Ala His170 175 180agc ctt ccc gaa aaa atc gtc gaa atg ggt gat ccg tat cct gaa cag 810Ser Leu Pro Glu Lys Ile Val Glu Met Gly Asp Pro Tyr Pro Glu Gln185 190 195ctt aaa gaa tca gcg aag ctg att gcc gaa gcg gcg gga gta aaa gat 858Leu Lys Glu Ser Ala Lys Leu Ile Ala Glu Ala Ala Gly Val Lys Asp200 205 210 215tac gcc gtc ggc tgg caa agc gaa gga aac acc cct gat ccg tgg ctg 906Tyr Ala Val Gly Trp Gln Ser Glu Gly Asn Thr Pro Asp Pro Trp Leu220 225 230gga ccg gat gtt cag gat ttg acg cgc gac ttg tca gag caa aaa ggg 954Gly Pro Asp Val Gln Asp Leu Thr Arg Asp Leu Ser Glu Gln Lys Gly235 240 245tac agc gct ttt gtt tat gct ccc gtc ggc ttt gta gcc gac cat ttg1002Tyr Ser Ala Phe Val Tyr Ala Pro Val Gly Phe Val Ala Asp His Leu250 255 260gaa gtc ctt tat gac aat gac tat gag tgc aaa gtc gtc acg gac gat1050Glu Val Leu Tyr Asp Asn Asp Tyr Glu Cys Lys Val Val Thr Asp Asp265 270 275
atc gga gca agc tat tac aga cct gaa atg cct aac gct aaa cat gag1098Ile Gly Ala Ser Tyr Tyr Arg Pro Glu Met Pro Asn Ala Lys His Glu280 285 290 295ttt atc gat gct tta gcg gat gtt gta tta aaa caa ttg gag aaa gaa1146Phe Ile Asp Ala Leu Ala Asp Val Val Leu Lys Gln Leu Glu Lys Glu300 305 310cag taaagaaggc gattgtaaat gagtggagaa cgcaggcaga ttgtcatcat 1199Glncggcggcggt attaccgggt tggccgccgc cttctacttg gagaaggaaa tcaaaaagaa 1259tgatctcccg gccgaagtca cgcttgttga agcaagcccg agactgggcg gaaagatgca 1319gacggtacac aaagacggct atgtgattga acggggtccg gattcatttt tggaacggaa 1379acaaagcgca cctcagctcg tgaaggatct cggactggag catttgcttg tgaacaatgc 1439gactggccag tcttatgtgc ttgtcagcga aacgctctat ccgattccgg aaggagccgt 1499gatgggggtg ccgacaaaaa tcggtccttt tctgacaacc ggcttgtttt ccttgccggg 1559gaagttgaga gccggaatgg atttggtgct tccagcaagc aagccgcagg aagaccaatc 1619gctcggagaa tttttcagaa ggcgcgtcgg cggtgaagtg gtggagaacc tgatcgaacc 1679gcttttgtca ggcatttatg ccggggacat cgaccgtctc agtctgatgt cgactttccc 1739gcagttctat cagacagagc agaagtacag aagcttgatc atcgggatga aaaaatcgcg 1799cggcgcccag agcgtcggaa agcctgcaaa caaaaagaaa ggccagttcc agacggtgaa 1859aaccggcctg cagacgatag ttgaagaatt ggaaaagcag ctcggcatca caaaggttta 1919taaaggcaca aaggtcgtca gcattgagcg tgcgggctcg gcttacagca tgaagcttga 1979caacggcaag gtgcttcacg ccgattcggc cgtggtcacc gctcctcata aagcagctgc 2039cgcgatgttt caaaaagagg attggctgaa gggtttagag gatatggtgt caacatctgt 2099cgccaatgtg gcgctcggat ttccggaaga agccgtccaa atggagcatc aaggcacggg 2159ctttgtcatt tcaagaaaca gcgatt 2185<210>24<211>312<212>PRT<213>地衣芽孢桿菌<400>24Leu Gly Lys Lys Lys Met Gly Leu Leu Val Met Ala Tyr Gly Thr Pro1 5 10 15Tyr Lys Glu Glu Asp Ile Glu Arg Tyr Tyr Thr His Ile Arg Arg Gly20 25 30Arg Lys Pro Glu Pro Asp Met Leu Gln Asp Leu Lys Asp Arg Tyr Lys35 40 45Ala Ile Gly Gly Ile Ser Pro Leu Ala Lys Ile Thr Gln Glu Gln Thr
50 55 60Lys Gln Leu Glu Lys Arg Leu Asn Glu Leu Gln Asp Asp Val Val Phe65 70 75 80Lys Ala Tyr Ile Gly Leu Lys His Ile Glu Pro Phe Ile Glu Asp Ala85 90 95Val Arg Glu Met His Lys Asp Gly Ile Thr Glu Ala Val Ser Ile Val100 105 110Leu Ala Pro His Phe Ser Thr Phe Ser Val Gln Ser Tyr Asn Lys Arg115 120 125Ala Lys Asp Glu Ala Glu Lys Leu Gly Glu Leu Ser Ile Thr Ser Ile130 135 140Asn Ser Trp Tyr Asp Glu Pro Lys Phe Ile Ala Tyr Trp Ala Asp Gln145 150 155 160Val Arg Lys Ile Tyr Asp Glu Met Pro Ser Glu Glu Arg Glu Asn Ala165 170 175Val Leu Ile Val Ser Ala His Ser Leu Pro Glu Lys Ile Val Glu Met180 185 190Gly Asp Pro Tyr Pro Glu Gln Leu Lys Glu Ser Ala Lys Leu Ile Ala195 200 205Glu Ala Ala Gly Val Lys Asp Tyr Ala Val Gly Trp Gln Ser Glu Gly210 215 220Asn Thr Pro Asp Pro Trp Leu Gly Pro Asp Val Gln Asp Leu Thr Arg225 230 235 240Asp Leu Ser Glu Gln Lys Gly Tyr Ser Ala Phe Val Tyr Ala Pro Val245 250 255Gly Phe Val Ala Asp His Leu Glu Val Leu Tyr Asp Asn Asp Tyr Glu260 265 270Cys Lys Val Val Thr Asp Asp Ile Gly Ala Ser Tyr Tyr Arg Pro Glu275 280 285Met Pro Asn Ala Lys His Glu Phe Ile Asp Ala Leu Ala Asp Val Val290 295 300Leu Lys Gln Leu Glu Lys Glu Gln305 310<210>25
<211>2107<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1604)<400>25aaaaaagctt ggcatagcca agctttctat tatgcagaaa gtccgttcac ttttttcgct 60aggtttgatt tgtaacgagc ggcggtgttt ttgtggacaa gacctttttt agctgcttta 120tcgattttct ttgctgctgc agatagagct gctttcgctt tgtctgcttc gttgttgctt 180acgaaagttt caacttgttt catagcagtg cgcattgcag atttgattgt tgcgttgtgc 240gcgcggcgct cgttgtttgt ttttgtgcgt ttgatcgctg atttaatgtt tggcaatgtg 300tttcacctcc taagacataa cctctgttcc ttttctatag gttagatctc aacaatagaa 360caaattgtat tctatcaaac acacccttag attgcaatat aaatgtaaag tatttttatt 420tgaaagctcc gcttcgcggg aatgtttgca gaccttgtgg gcacactaac accagttatg 480tttttggagg gaatgcaaat atg gag aaa aag aag ctc gat tta agt caa tat 533Met Glu Lys Lys Lys Leu Asp Leu Ser Gln Tyr1 5 10gca gtc cgg aca gac tta gcc gtg gaa gcg agg gat ctg gca gag gaa581Ala Val Arg Thr Asp Leu Ala Val Glu Ala Arg Asp Leu Ala Glu Glu15 20 25aag gaa gct tct cca aag aaa gag ctg aaa ggg ttt aca gtc aaa gaa629Lys Glu Ala Ser Pro Lys Lys Glu Leu Lys Gly Phe Thr Val Lys Glu30 35 40tac gaa aaa gac ggg atc aaa att cag acg atg gat att gat gaa gaa677Tyr Glu Lys Asp Gly Ile Lys Ile Gln Thr Met Asp Ile Asp Glu Glu45 50 55gga gcg aag ctg tcg ggg aaa aaa gcc ggc cgc tat tta acc ttt gaa725Gly Ala Lys Leu Set Gly Lys Lys Ala Gly Arg Tyr Leu Thr Phe Glu60 65 70 75aca caa ggg atc aga cag cag gat tcc gta ttg cag gaa aaa gtc gtc773Thr Gln Gly Ile Arg Gln Gln Asp Ser Val Leu Gln Glu Lys Val Val80 85 90gat gtc ttc gca aag gaa ttt tcc tcg ttt ctc gat tat ttg ggc att821Asp Val Phe Ala Lys Glu Phe Ser Ser Phe Leu Asp Tyr Leu Gly Ile95 100 105ccg cgg gat gcc agc tgc tta ata gtc ggg ctc ggc aac tgg aat gtc869Pro Arg Asp Ala Ser Cys Leu Ile Val Gly Leu Gly Asn Trp Asn Val110 115 120acg ccc gac tcg ctc ggc ccc cta gtg acg gag aac ctt ctc gtg acg917Thr Pro Asp Ser Leu Gly Pro Leu Val Thr Glu Asn Leu Leu Val Thr125 130 135agg cac ctg ttt cag ctg cag ccc gaa aat gta gag gaa ggc tac cgt965Arg His Leu Phe Gln Leu Gln Pro Glu Asn Val Glu Glu Gly Tyr Arg140 145 150 155ccg gtg agt gcg ctt tcc ccg gga gtc atg ggc ttg acc gga att gaa 1013Pro Val Ser Ala Leu Ser Pro Gly Val Met Gly Leu Thr Gly Ile Glu160 165 170
acg agc gac att atc caa ggg gtg atc gat cgc tca aag ccc gat ttt1061Thr Ser Asp Ile Ile Gln Gly Val Ile Asp Arg Ser Lys Pro Asp Phe175 180 185gtc att gcc att gat gcg ctg gca tca aga ggg atc gag cgt gtg aat1109Val Ile Ala Ile Asp Ala Leu Ala Ser Arg Gly Ile Glu Arg Val Asn190 195 200tct acc att caa att tca gac agc ggg ata cac ccc ggg tct ggt gtg1157Ser Thr Ile Gln Ile Ser Asp Ser Gly Ile His Pro Gly Ser Gly Val205 210 215ggg aat aaa aga aaa gag tta agc aag gat acg ctc ggc att ccg gtc1205Gly Asn Lys Arg Lys Glu Leu Ser Lys Asp Thr Leu Gly Ile Pro Val220 225 230 235atc gcc atc ggt gtt ccg aca gtg gtc gat gcc gtc acg atc acg agc1253Ile Ala Ile Gly Val Pro Thr Val Val Asp Ala Val Thr Ile Thr Ser240 245 250gac acg att gat tac atg ctg aag cat ttt gga aga gag atg aga gac1301Asp Thr Ile Asp Tyr Met Leu Lys His Phe Gly Arg Glu Met Arg Asp255 260 265gac agt ccg tcc agg tcg ctc gtt ccc gcg ggg atg agc ttt ggg aaa1349Asp Ser Pro Ser Arg Ser Leu Val Pro Ala Gly Met Ser Phe Gly Lys270 275 280aga aag gtc ctc acc gaa gaa gac ctt ccc gat gaa gag cac cgc aaa1397Arg Lys Val Leu Thr Glu Glu Asp Leu Pro Asp Glu Glu His Arg Lys285 290 295tcg ttt ctc ggt ata gtc gga ggc ctc gca gag gat gaa aaa agg cag1445Ser Phe Leu Gly Ile Val Gly Gly Leu Ala Glu Asp Glu Lys Arg Gln300 305 310 315ctg atc cat gaa gtc ctc gct ccg ctc ggc cat aac tta atg gtg acc1493Leu Ile His Glu Val Leu Ala Pro Leu Gly His Asn Leu Met Val Thr320 325 330cct aaa gag gtc gat aca ttt atc gat gat atg gca aat gtg att gca1541Pro Lys Glu Val Asp Thr Phe Ile Asp Asp Met Ala Asn Val Ile Ala335 340 345aac ggt ctg aac aca gcg ctc cat gaa aat gta tcg caa gac aat aaa1589Asn Gly Leu Asn Thr Ala Leu His Glu Asn Val Ser Gln Asp Asn Lys350 355 360gga atg tat aac cac taagatcaag gcggctggcc cggccgcttt ttttcatgac1644Gly Met Tyr Asn His365atttagcatt gccggaactt gttctacttc ctctatcttg tacatagtct atttactaga 1704caagctctgg agggatttac aatgagaaaa agaggcagga atcgtcagtt tgtgctggct l764gtaaatggaa gaagcgcggt taagacagta ttcttattta tcgtcagcct gctgctcgtt 1824tttattttat caggcgtgct cacatcgctg cgtcctgagt taaggccgtc ttcatcgttg 1884taccgggtgg ctgatgaact gaagggcgag acctttgggc tcattttggg aatggaaaac 1944cactactttg catcagaatt gccggaaccg aataagcgct ttgagctttc ccccctcgtc 2004ctgaagctgg cgaccagcat taatttgaaa gatccgcgaa gttttctcgg acgggagctt 2064ccgggattct ctcattttga ctcggaaatc ctcattgccg ggc2107
<2l0>26<211>368<212>PRT<213>地衣芽孢桿菌<400>26Met Glu Lys Lys Lys Leu Asp Leu Ser Gln Tyr Ala Val Arg Thr Asp1 5 10 15Leu Ala Val Glu Ala Arg Asp Leu Ala Glu Glu Lys Glu Ala Ser Pro20 25 30Lys Lys Glu Leu Lys Gly Phe Thr Val Lys Glu Tyr Glu Lys Asp Gly35 40 45Ile Lys Ile Gln Thr Met Asp Ile Asp Glu Glu Gly Ala Lys Leu Ser50 55 60Gly Lys Lys Ala Gly Arg Tyr Leu Thr Phe Glu Thr Gln Gly Ile Arg65 70 75 80Gln Gln Asp Ser Val Leu Gln Glu Lys Val Val Asp Val Phe Ala Lys85 90 95Glu Phe Ser Ser Phe Leu Asp Tyr Leu Gly Ile Pro Arg Asp Ala Ser100 105 110Cys Leu Ile Val Gly Leu Gly Asn Trp Asn Val Thr Pro Asp Ser Leu115 120 125Gly Pro Leu Val Thr Glu Asn Leu Leu Val Thr Arg His Leu Phe Gln130 135 140Leu Gln Pro Glu Asn Val Glu Glu Gly Tyr Arg Pro Val Ser Ala Leu145 150 155 160Ser Pro Gly Val Met Gly Leu Thr Gly Ile Glu Thr Ser Asp Ile Ile165 170 175Gln Gly Val Ile Asp Arg Ser Lys Pro Asp Phe Val Ile Ala Ile Asp180 185 190Ala Leu Ala Ser Arg Gly Ile Glu Arg Val Asn Ser Thr Ile Gln Ile195 200 205Ser Asp Ser Gly Ile His Pro Gly Ser Gly Val Gly Asn Lys Arg Lys210 215 220Glu Leu Ser Lys Asp Thr Leu Gly Ile Pro Val Ile Ala Ile Gly Val225 230 235 240
Pro Thr Val Val Asp Ala Val Thr Ile Thr Ser Asp Thr Ile Asp Tyr245 250 255Met Leu Lys His Phe Gly Arg Glu Met Arg Asp Asp Ser Pro Ser Arg260 265 270Ser Leu Val Pro Ala Gly Met Ser Phe Gly Lys Arg Lys Val Leu Thr275 280 285Glu Glu Asp Leu Pro Asp Glu Glu His Arg Lys Ser Phe Leu Gly Ile290 295 300Val Gly Gly Leu Ala Glu Asp Glu Lys Arg Gln Leu Ile His Glu Val305 310 315 320Leu Ala Pro Leu Gly His Asn Leu Met Val Thr Pro Lys Glu Val Asp325 330 335Thr Phe Ile Asp Asp Met Ala Asn Val Ile Ala Asn Gly Leu Asn Thr340 345 350Ala Leu His Glu Asn Val Ser Gln Asp Asn Lys Gly Met Tyr Asn His355 360 365<210>27<211>2358<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1868)<400>27tgtcaatgtc ggcatcgcgc gcagaaataa aaatgacggg gacttccgga tcttgatttt 60ttatttcttt tattagcgta tacccgtcga tatctggtaa catgatatcg agaatccata 120aatgaggtga ctggcctatt ttttgcctgg cgctttcacc gtttagaaaa gaagtgacgt 180gccagccttc actctctaag tattttgtca gcaattcatt taaattctct tcatcttcaa 240ctagataaat ggagtatgac aagggcggtt cacatccttt ctacgtcatt atactagttt 300taacataagt ctggtcaatt ttcataattt cacatattct tttcattttt atcccacaat 360gttttcgtaa catattcatc agggaagaaa gggaatgaaa acagagtgaa cgatggccgg 420gtctcttttt ttgtggatgt taaagcttta atgccatttg cgatagttga agacaagaaa 480aataaaggaa gtgtacgctt atg gat ttt aga cgc gat gac gaa caa aaa cat 533Met Asp Phe Arg Arg Asp Asp Glu Gln Lys His1 5 10gca aat gaa gag cag ttt cac gaa gaa gcg cag acg ccg caa acg gca581Ala Asn Glu Glu Gln Phe His Glu Glu Ala Gln Thr Pro Gln Thr Ala15 20 25ggt tct gag gag cag cag tca gaa cag ccg gca aag gag ccc gag ctg629Gly Ser Glu Glu Gln Gln Ser Glu Gln Pro Ala Lys Glu Pro Glu Leu
30 35 40att tta aag aag gat aca gac gat cag tca gat ctt acg aat aaa gaa 677Ile Leu Lys Lys Asp Thr Asp Asp Gln Ser Asp Leu Thr Asn Lys Glu45 50 55acc gct gca gcc ctg gaa gat gcc agg gaa acg aga gcg gca aaa gaa 725Thr Ala Ala Ala Leu Glu Asp Ala Arg Glu Thr Arg Ala Ala Lys Glu60 65 70 75aag cgg cgc aag gct tcg tgg ctg agc ccg att ttg ggc ggg atc atc 773Lys Arg Arg Lys Ala Ser Trp Leu Ser Pro Ile Leu Gly Gly Ile Ile80 85 90gga ggc ggc ttg gtg ctt gga atc tcc ccg tat ctg ccg ggc gat cat 821Gly Gly Gly Leu Val Leu Gly Ile Ser Pro Tyr Leu Pro Gly Asp His95 100 105ccg agc gaa act gcc gga acc gga cag aca gag cca aag cag tcg cag 869Pro Ser Glu Thr Ala Gly Thr Gly Gln Thr Glu Pro Lys Gln Ser Gln110 115 120aat ttc acc aca aaa cct gtc acg aac gca gat aac gta cct gat atg 917Asn Phe Thr Thr Lys Pro Val Thr Asn Ala Asp Asn Val Pro Asp Met125 130 135gtg gaa gat ttg gag cct gcg atc gtc gga gtt tca aat att caa acg 965Val Glu Asp Leu Glu Pro Ala Ile Val Gly Val Ser Asn Ile Gln Thr140 145 150 155agc ttc ggc ttc tct gaa gac gac gtg gag gag agc ggc acg gga tca1013Ser Phe Gly Phe Ser Glu Asp Asp Val Glu Glu Ser Gly Thr Gly Ser160 165 170ggc gtc atc ttt aag aaa gac ggc ggc aag gcc tac att atc aca aac1061Gly Val Ile Phe Lys Lys Asp Gly Gly Lys Ala Tyr Ile Ile Thr Asn175 180 185aac cat gtc gtt gaa ggc gcc tcg aag gtg acc atc tcc tta tac aac1109Asn His Val Val Glu Gly Ala Ser Lys Val Thr Ile Ser Leu Tyr Asn190 195 200gga aag acc gcg gat gcc aag att atc ggc agc gac gct ttg act gat1157Gly Lys Thr Ala Asp Ala Lys Ile Ile Gly Ser Asp Ala Leu Thr Asp205 210 215ctg gct gtt tta gag atc agc agc aaa ggc gtt gat aag gtt gca agt1205Leu Ala Val Leu Glu Ile Ser Ser Lys Gly Val Asp Lys Val Ala Ser220 225 230 235ttc gga gac tcc gca aaa ctg cgc gcc ggc gaa aaa gta att gcg atc1253Phe Gly Asp Ser Ala Lys Leu Arg Ala Gly Glu Lys Val Ile Ala Ile240 245 250ggg aac cct ctc ggc ctt caa ttt tca aga acg gtt aca gaa ggc atc1301Gly Asn Pro Leu Gly Leu Gln Phe Ser Arg Thr Val Thr Glu Gly Ile255 260 265atc agc ggt gtc aac cgg acg att gaa gtc tca aca tct gaa ggg aat1349Ile Ser Gly Val Asn Arg Thr Ile Glu Val Ser Thr Ser Glu Gly Asn270 275 280tgg gat atg aac gtc ctt cag aca gat gcg gcg atc aac cct gga aac1397Trp Asp Met Asn Val Leu Gln Thr Asp Ala Ala Ile Asn Pro Gly Asn285 290 295agc ggc ggg cct ctg att aac agc agc ggg cag gtc atc ggc atc aac1445Ser Gly Gly Pro Leu Ile Asn Ser Ser Gly Gln Val Ile Gly Ile Asn300 305 310 315
agc ctg aag atc agc caa agc ggt gtt gaa tcg ctt ggc ttc gcg att1493Ser Leu Lys Ile Ser Gln Ser Gly Val Glu Ser Leu Gly Phe Ala Ile320 325 330ccg agc aac gat gtt cag ccg atc gtt gac gag ctt ttg gaa aaa ggg1541Pro Ser Asn Asp Val Gln Pro Ile Val Asp Glu Leu Leu Glu Lys Gly335 340 345aaa gtg gaa cgg cct ttc ctc ggc gtg caa atg atc gat atg cag cag1589Lys Val Glu Arg Pro Phe Leu Gly Val Gln Met Ile Asp Met Gln Gln350 355 360gtg cct gaa cag tac cag caa aat acg ctc ggc cta ttc gga gac cag1637Val Pro Glu Gln Tyr Gln Gln Asn Thr Leu Gly Leu Phe Gly Asp Gln365 370 375ctt aat aaa ggc gtg tac atc gat aaa gta tcg ccg aaa tcg cct gcg1685Leu Asn Lys Gly Val Tyr Ile Asp Lys Val Ser Pro Lys Ser Pro Ala380 385 390 395gca gac gcg ggt atg aaa gcc ggc gac gtc atc acc aaa atg aac ggc1733Ala Asp Ala Gly Met Lys Ala Gly Asp Val Ile Thr Lys Met Asn Gly400 405 410aaa aac gtc gaa acc acc tca gac ctc aga aaa att ctt tat aca gaa1781Lys Asn Val Glu Thr Thr Ser Asp Leu Arg Lys Ile Leu Tyr Thr Glu415 420 425gca aaa gca gga gat act gtc aca ttt gag gtg ctg aga aac gga aag1829Ala Lys Ala Gly Asp Thr Val Thr Phe Glu Val Leu Arg Asn Gly Lys430 435 440caa aca aca atg aaa gca aaa ctt gcg aaa agc aaa tca taaataaaag 1878Gln Thr Thr Met Lys Ala Lys Leu Ala Lys Ser Lys Ser445 450 455ccatggcaaa cgccatggct ttttcatatt acttgtccaa aaatgaagaa agcatccaaa 1938tctgcttatc aatttcctct gtcagggcga tataaaggtc agcggtagaa tgatctgatt 1998tatcttccgc aagttcgatg gtatgctgaa tttcatcgcg gatttgacgg taatcgctta 2058ccagtgcgga aaccatttct tcggccgttt tttcggctcc gttttcctca attgtgccgt 2118ggtcaatgta ttctttcatt gtggcgagcg gctgtccgcc gatggccagc aagcgttccg 2178cgatcacatc tgctgtttct gacgcatggt tgtatagctc ttcaaatttt tcatgtaggg 2238taaagaactg cggccctttt acgtaccagt gaaaacggtg aagcttcgtg taaaggatga 2298accagtttga caaattcgta ttcattgagt tttcaagtac aggatttgct gttttaattt 2358<210>28<211>456<212>PRT<213>地衣芽孢桿菌<400>28Met Asp Phe Arg Arg Asp Asp Glu Gln Lys His Ala Asn Glu Glu Gln1 5 10 15Phe His Glu Glu Ala Gln Thr Pro Gln Thr Ala Gly Ser Glu Glu Gln20 25 30
Gln Ser Glu Gln Pro Ala Lys Glu Pro Glu Leu Ile Leu Lys Lys Asp35 40 45Thr Asp Asp Gln Ser Asp Leu Thr Asn Lys Glu Thr Ala Ala Ala Leu50 55 60Glu Asp Ala Arg Glu Thr Arg Ala Ala Lys Glu Lys Arg Arg Lys Ala65 70 75 80Ser Trp Leu Ser Pro Ile Leu Gly Gly Ile Ile Gly Gly Gly Leu Val85 90 95Leu Gly Ile Ser Pro Tyr Leu Pro Gly Asp His Pro Ser Glu Thr Ala100 105 110Gly Thr Gly Gln Thr Glu Pro Lys Gln Ser Gln Asn Phe Thr Thr Lys115 120 125Pro Val Thr Asn Ala Asp Asn Val Pro Asp Met Val Glu Asp Leu Glu130 135 140Pro Ala Ile Val Gly Val Ser Asn Ile Gln Thr Ser Phe Gly Phe Ser145 150 155 160Glu Asp Asp Val Glu Glu Ser Gly Thr Gly Ser Gly Val Ile Phe Lys165 170 175Lys Asp Gly Gly Lys Ala Tyr Ile Ile Thr Asn Asn His Val Val Glu180 185 190Gly Ala Ser Lys Val Thr Ile Ser Leu Tyr Asn Gly Lys Thr Ala Asp195 200 205Ala Lys Ile Ile Gly Ser Asp Ala Leu Thr Asp Leu Ala Val Leu Glu210 215 220Ile Ser Ser Lys Gly Val Asp Lys Val Ala Ser Phe Gly Asp Ser Ala225 230 235 240Lys Leu Arg Ala Gly Glu Lys Val Ile Ala Ile Gly Asn Pro Leu Gly245 250 255Leu Gln Phe Ser Arg Thr Val Thr Glu Gly Ile Ile Ser Gly Val Asn260 265 270Arg Thr Ile Glu Val Ser Thr Ser Glu Gly Asn Trp Asp Met Asn Val275 280 285Leu Gln Thr Asp Ala Ala Ile Asn Pro Gly Asn Ser Gly Gly Pro Leu290 295 300Ile Asn Ser Ser Gly Gln Val Ile Gly Ile Asn Ser Leu Lys Ile Ser
305 310 315 320Gln Ser Gly Val Glu Ser Leu Gly Phe Ala Ile Pro Ser Asn Asp Val325 330 335Gln Pro Ile Val Asp Glu Leu Leu Glu Lys Gly Lys Val Glu Arg Pro340 345 350Phe Leu Gly Val Gln Met Ile Asp Met Gln Gln Val Pro Glu Gln Tyr355 360 365Gln Gln Asn Thr Leu Gly Leu Phe Gly Asp Gln Leu Asn Lys Gly Val370 375 380Tyr Ile Asp Lys Val Ser Pro Lys Ser Pro Ala Ala Asp Ala Gly Met385 390 395 400Lys Ala Gly Asp Val Ile Thr Lys Met Asn Gly Lys Asn Val Glu Thr405 410 415Thr Ser Asp Leu Arg Lys Ile Leu Tyr Thr Glu Ala Lys Ala Gly Asp420 425 430Thr Val Thr Phe Glu Val Leu Arg Asn Gly Lys Gln Thr Thr Met Lys435 440 445Ala Lys Leu Ala Lys Ser Lys Ser450 455<210>29<211>1647<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1559)<400>29cactgtaagt ggacattttt ttgcgtgatg agaaggggga ggcagagttg ccgcatattt60taattttgaa cggaccgaat ttaaaccggc ttggaaaacg cgaacccgat gtatacggga 120cagacacatt aaccgatttg gaacaaagac tttttcaatt tgccgaaggc attcagacag 180aattgacttt ttttcagtca aaccacgaag gggacctgat tgatgctctt catgaggcag 240aggagcaata cgatggaatc gtcttgaatc cgggggcttt ctcgcattac agctatgccc 300tccgcgatgc ggtggctgca atcagcatac cggtgattga ggttcatctt tcaaatcctc 360atgcaaggga agagttccgg caccgctcgg tcatcgctcc ggttgcaaga ggccagatca 420ccggactcgg ctttgaaggt tataagctgg ccatctctta ttttatgaac acaaataaca 480aataggggga ctagtggaca atg aaa cta aac aaa ttg aga gag ctt ttc ggc 533Met Lys Leu Asn Lys Leu Arg Glu Leu Phe Gly
1 5 10ggt tta ggc att gac ggt ata ttg gtg acg agc ggc gtt aat ttg cgg 581Gly Leu Gly Ile Asp Gly Ile Leu Val Thr Ser Gly Val Asn Leu Arg15 20 25tac atc acc gga ttt acc gga tct tca gga ctg gcg gtc atc tca gac 629Tyr Ile Thr Gly Phe Thr Gly Ser Ser Gly Leu Ala Val Ile Ser Asp30 35 40gat caa gca gtc ttt att acg gat ttc cgc tat aca gaa cag gcg aag 677Asp Gln Ala Val Phe Ile Thr Asp Phe Arg Tyr Thr Glu Gln Ala Lys45 50 55gat caa atc aaa agc ttt gac atc gtt cag cat gcc ggc gga atc gtt 725Asp Gln Ile Lys Ser Phe Asp Ile Val Gln His Ala Gly Gly Ile Val60 65 70 75caa aag acc gcc gaa gtg atc aaa gag atg ggg ata aac aaa atc ggt 773Gln Lys Thr Ala Glu Val Ile Lys Glu Met Gly Ile Asn Lys Ile Gly80 85 90ttt gaa cag gat aaa atg aca tac gga acg tat gcc gcc tac aaa gag 821Phe Glu Gln Asp Lys Met Thr Tyr Gly Thr Tyr Ala Ala Tyr Lys Glu95 100 105cag ctt ggc gca gct gaa ctc gtt ccc gtt tct caa tct gtg gaa aag 869Gln Leu Gly Ala Ala Glu Leu Val Pro Val Ser Gln Ser Val Glu Lys110 115 120ttg cgc ttg att aag tca agt gaa gag att aag ata tta aag gaa gct 917Leu Arg Leu Ile Lys Ser Ser Glu Glu Ile Lys Ile Leu Lys Glu Ala125 130 135gca aag att gca gat gat gca ttc agc cat att ctg acg gtc atc aag 965Ala Lys Ile Ala Asp Asp Ala Phe Ser His Ile Leu Thr Val Ile Lys140 145 150 155ccc ggc atc acc gaa att gaa gtc gcc aat gag ctc gag ttt tac atg1013Pro Gly Ile Thr Glu Ile Glu Val Ala Asn Glu Leu Glu Phe Tyr Met160 165 170cgg agc cag gga gcc gat cat tcg tcc ttc gat atg atc gtg gcg tca1061Arg Ser Gln Gly Ala Asp His Ser Ser Phe Asp Met Ile Val Ala Ser175 180 185ggc gta cgc tca agt ctg ccg cac gga gtg gca agc ggc aaa gcg atc1109Gly Val Arg Ser Ser Leu Pro His Gly Val Ala Ser Gly Lys Ala Ile190 195 200gag aaa ggc gac ctt gtg acg ctg gac ttt ggg gct tac tat aaa ggt1157Glu Lys Gly Asp Leu Val Thr Leu Asp Phe Gly Ala Tyr Tyr Lys Gly205 210 215tac tgc tcg gac att acg cgt aca gtc gcg gta gga gag ccg gat gat1205Tyr Cys Ser Asp Ile Thr Arg Thr Val Ala Val Gly Glu Pro Asp Asp220 225 230 235gag ctg aaa cgc att tat caa acc gtg ttt gag gcg cag gct atc ggc1253Glu Leu Lys Arg Ile Tyr Gln Thr Val Phe Glu Ala Gln Ala Ile Gly240 245 250atg cgg agc atc aag ccg gga atc acc ggg aaa cag gct gac gca tac1301Met Arg Ser Ile Lys Pro Gly Ile Thr Gly Lys Gln Ala Asp Ala Tyr255 260 265aca agg gac tac ata tct tca caa ggc tac ggc gat tat ttc ggc cat1349Thr Arg Asp Tyr Ile Ser Ser Gln Gly Tyr Gly Asp Tyr Phe Gly His270 275 280
tca aca ggc cat ggc ctc ggc atg gaa gtt cat gaa agt ecg gcg ctt1397Ser Thr Gly His Gly Leu Gly Met Glu Val His Glu Ser Pro Ala Leu285 290 295tca gca cgt tct gac cag atg ctt gaa aaa ggc atg gtt gta acg gtt1445Ser Ala Arg Ser Asp Gln Met Leu Glu Lys Gly Met Val Val Thr Val300 305 310 315gaa ccg gga att tac ata ccg ggc aaa ggc ggg gtc agg ata gaa gat1493Glu Pro Gly Ile Tyr Ile Pro Gly Lys Gly Gly Val Arg Ile Glu Asp320 325 330gat att gtc ctg aca gag gaa gga aac gaa tcc ctt acc cat tcc gcg1541Asp Ile Val Leu Thr Glu Glu Gly Asn Glu Ser Leu Thr His Ser Ala335 340 345aaa gat tta att att tta taatccgttt gattggagca ataggaggat 1589Lys Asp Leu Ile Ile Leu350aatcatgatt tcagtaaacg atttcgcaca gtttaagtat caataaggga gtttctaa1647<210>30<211>353<212>PRT<213>地衣芽孢桿菌<400>30Met Lys Leu Asn Lys Leu Arg Glu Leu Phe Gly Gly Leu Gly Ile Asp1 5 10 15Gly Ile Leu Val Thr Ser Gly Val Asn Leu Arg Tyr Ile Thr Gly Phe20 25 30Thr Gly Ser Ser Gly Leu Ala Val Ile Ser Asp Asp Gln Ala Val Phe35 40 45Ile Thr Asp Phe Arg Tyr Thr Glu Gln Ala Lys Asp Gln Ile Lys Ser50 55 60Phe Asp Ile Val Gln His Ala Gly Gly Ile Val Gln Lys Thr Ala Glu65 70 75 80Val Ile Lys Glu Met Gly Ile Asn Lys Ile Gly Phe Glu Gln Asp Lys85 90 95Met Thr Tyr Gly Thr Tyr Ala Ala Tyr Lys Glu Gln Leu Gly Ala Ala100 105 110Glu Leu Val Pro Val Ser Gln Ser Val Glu Lys Leu Arg Leu Ile Lys115 120 125Ser Ser Glu Glu Ile Lys Ile Leu Lys Glu Ala Ala Lys Ile Ala Asp130 135 140Asp Ala Phe Ser His Ile Leu Thr Val Ile Lys Pro Gly Ile Thr Glu
145 150 155 160Ile Glu Val Ala Asn Glu Leu Glu Phe Tyr Met Arg Ser Gln Gly Ala165 170 175Asp His Ser Ser Phe Asp Met Ile Val Ala Ser Gly Val Arg Ser Ser180 185 190Leu Pro His Gly Val Ala Ser Gly Lys Ala Ile Glu Lys Gly Asp Leu195 200 205Val Thr Leu Asp Phe Gly Ala Tyr Tyr Lys Gly Tyr Cys Ser Asp Ile210 215 220Thr Arg Thr Val Ala Val Gly Glu Pro Asp Asp Glu Leu Lys Arg Ile225 230 235 240Tyr Gln Thr Val Phe Glu Ala Gln Ala Ile Gly Met Arg Ser Ile Lys245 250 255Pro Gly Ile Thr Gly Lys Gln Ala Asp Ala Tyr Thr Arg Asp Tyr Ile260 265 270Ser Ser Gln Gly Tyr Gly Asp Tyr Phe Gly His Ser Thr Gly His Gly275 280 285Leu Gly Met Glu Val His Glu Ser Pro Ala Leu Ser Ala Arg Ser Asp290 295 300Gln Met Leu Glu Lys Gly Met Val Val Thr Val Glu Pro Gly Ile Tyr305 310 315 320Ile Pro Gly Lys Gly Gly Val Arg Ile Glu Asp Asp Ile Val Leu Thr325 330 335Glu Glu Gly Asn Glu Ser Leu Thr His Ser Ala Lys Asp Leu Ile Ile340 345 350Leu<210>31<211>1941<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1451)<400>31gcaaaaaaat cgaaaagccg gaaattacaa agctcacaga catcatcgat acagatttga 60tcgaacctca tttgtcagga gatacgagag acgacattat tgatgaaatg atccaaaaat120
tgtcccggaa aggcgtactg cattcagaaa gcgaatttaa acaagctatc atcaatcgcg 180aacttgaggg tacaacggcg atcggtatga atatcgcgat cccgcacggg aagtctgatg 240ccgtcaagaa gccgagtgta gcattcggca ttaaacgttc gggcgtagac tggaggagtc 300ttgacggaac tgacgccaaa ctgattttta tgatcgccgt cccgaaagaa agcggaggaa 360acgagcatct gaagcttctg caaatgctct ccagaaaact gatggatgac acctaccggg 420acaagcttct ctctgcccaa acaaaagaag aagcatacaa acttctggat gaaatcatct 480aaaaaaggag gacgcccgtc atg act gaa ccg tta ttt ttc gag cct gtt ttt 533Met Thr Glu Pro Leu Phe Phe Glu Pro Val Phe1 5 10aaa gaa aga ata tgg ggc gga acg gct tta gcc tct ttc ggc tat gac581Lys Glu Arg Ile Trp Gly Gly Thr Ala Leu Ala Ser Phe Gly Tyr Asp15 20 25att cca tcc gaa caa aca ggg gag tgc tgg gct ttc gcc gca cat caa629Ile Pro Ser Glu Gln Thr Gly Glu Cys Trp Ala Phe Ala Ala His Gln30 35 40aat gga caa agc gtc gtg aaa aac ggc ttg tat aaa ggg ctc aca tta677Asn Gly Gln Ser Val Val Lys Asn Gly Leu Tyr Lys Gly Leu Thr Leu45 50 55agc gaa tta tgg gat gaa cac cgt cat tta ttc gga cat ata aag gga725Ser Glu Leu Trp Asp Glu His Arg His Leu Phe Gly His Ile Lys Gly60 65 70 75gac cgc ttt cct ctt ttg aca aaa att ttg gac gcc gac caa gac cta773Asp Arg Phe Pro Leu Leu Thr Lys Ile Leu Asp Ala Asp Gln Asp Leu80 85 90tcc gta caa gtg cat cca aat gat gaa tat gca aag ctg cac gaa aac821Ser Val Gln Val His Pro Asn Asp Glu Tyr Ala Lys Leu His Glu Asn95 100 105ggg gag ctt gga aag acg gaa tgc tgg tat atc att gat tgc aag gaa869Gly Glu Leu Gly Lys Thr Glu Cys Trp Tyr Ile Ile Asp Cys Lys Glu110 115 120gat gcg gag atc att tac ggc cac cat gcc aag acg cgg gaa gaa ctg917Asp Ala Glu Ile Ile Tyr Gly His His Ala Lys Thr Arg Glu Glu Leu125 130 135gtc tca atg atc gaa aac gga aaa tgg aat gag ctt ttg cgc cgc gtc965Val Ser Met Ile Glu Asn Gly Lys Trp Asn Glu Leu Leu Arg Arg Val140 145 150 155aaa gtg aag ccg gga gat ttc ttc tat gtg ccg agc gga acc gtc cat 1013Lys Val Lys Pro Gly Asp Phe Phe Tyr Val Pro Ser Gly Thr Val His160 165 170gcg atc gga aaa ggc att ttg att ttg gag acg cag cag aat tct gac 1061Ala Ile Gly Lys Gly Ile Leu Ile Leu Glu Thr Gln Gln Asn Ser Asp175 180 185aca acc tac aga cta tat gat tat gac aga aaa gac gct gaa gga aac 1109Thr Thr Tyr Arg Leu Tyr Asp Tyr Asp Arg Lys Asp Ala Glu Gly Asn190 195 200ctg cgc gag ctc cat ttg caa aaa agc ata gaa gtg atc gac gtt ccg 1157Leu Arg Glu Leu His Leu Gln Lys Ser Ile Glu Val Ile Asp Val Pro205 210 215
tct tcc cct gat cgc gaa acc gtt caa tac gaa aca tcg ggc gac gtc1205Ser Ser Pro Asp Arg Glu Thr Val Gln Tyr Glu Thr Ser Gly Asp Val220 225 230 235aag act gcc gca ctg att gat tgt cct tat ttc tca gta gaa aaa tgg1253Lys Thr Ala Ala Leu Ile Asp Cys Pro Tyr Phe Ser Val Glu Lys Trp240 245 250gat gta aaa gga tcg gcc tgt ttc aaa cag gat aaa ccg ttt ctt ctt1301Asp Val Lys Gly Ser Ala Cys Phe Lys Gln Asp Lys Pro Phe Leu Leu255 260 265gcc agc gtc ata gaa ggg gaa ggg cgc atc gca tct ggc ggc cat aac1349Ala Ser Val Ile Glu Gly Glu Gly Arg Ile Ala Ser Gly Gly His Asn270 275 280ttt tca ttt aaa aaa gga gat cac atg ctg ctg ccc tgg ggc ttt gga1397Phe Ser Phe Lys Lys Gly Asp His Met Leu Leu Pro Trp Gly Phe Gly285 290 295gaa ttt acg ctc gcc ggc cat gcg gaa tgt atc gtc tca agc tta tca1445Glu Phe Thr Leu Ala Gly His Ala Glu Cys Ile Val Ser Ser Leu Ser300 305 310 315att gaa taaacgatca gcggcgccct ttctgaacag accataaaac tatggtctgt 1501Ile Glutttatttttg caaaaaaaat aatctcgact tgccaagacg tgttcacaga gggctgacaa 1561tgcatattcc caagctttca caagtaaaat agagtaaacc aaaaaaacat ttggaaatct 1621tccaattatt tgataagctg atattaaaag cttcatatct ttcatcagct gcactgcccg 1681ggacttttcg cggaacagct gcttcatgaa acaaaaaggc ggtcctatgt tccggcgtct 1741ggcaagaaaa gcctgctaaa tgaccgaaac aaccgtgccc ttcataattg gttggtcctt 1801atctggatga gaaaaggaga cgatgattcg tatcagcaaa aatgtaagcg gattcatcgt 1861ctttttatct cggtttatca ctctttttag cggaagcgag gaggtaagca atggggaaag 1921caaaacttcg atggaatgaa 1941<210>32<211>317<212>PRT<213>地衣芽孢桿菌<400>32Met Thr Glu Pro Leu Phe Phe Glu Pro Val Phe Lys Glu Arg Ile Trp1 5 10 15Gly G1y Thr A1a Leu A1a Ser Phe Gly Tyr Asp Ile Pro Ser G1u Gln20 25 30Thr Gly Glu Cys Trp Ala Phe Ala A1a His G1n Asn Gly Gln Ser Val35 40 45Val Lys Asn G1y Leu Tyr Lys Gly Leu Thr Leu Ser Glu Leu Trp Asp50 55 60Glu His Arg His Leu Phe Gly His Ile Lys Gly Asp Arg Phe Pro Leu
65 70 75 80Leu Thr Lys Ile Leu Asp Ala Asp Gln Asp Leu Ser Val Gln Val His85 90 95Pro Asn Asp Glu Tyr Ala Lys Leu His Glu Asn Gly Glu Leu Gly Lys100 105 110Thr Glu Cys Trp Tyr Ile Ile Asp Cys Lys Glu Asp Ala Glu Ile Ile115 120 125Tyr Gly His His Ala Lys Thr Arg Glu Glu Leu Val Ser Met Ile Glu130 135 140Asn Gly Lys Trp Asn Glu Leu Leu Arg Arg Val Lys Val Lys Pro Gly145 150 155 160Asp Phe Phe Tyr Val Pro Ser Gly Thr Val His Ala Ile Gly Lys Gly165 170 175Ile Leu Ile Leu Glu Thr Gln Gln Asn Ser Asp Thr Thr Tyr Arg Leu180 185 190Tyr Asp Tyr Asp Arg Lys Asp Ala Glu Gly Asn Leu Arg Glu Leu His195 200 205Leu Gln Lys Ser Ile Glu Val Ile Asp Val Pro Ser Ser Pro Asp Arg210 215 220Glu Thr Val Gln Tyr Glu Thr Ser Gly Asp Val Lys Thr Ala Ala Leu225 230 235 240Ile Asp Cys Pro Tyr Phe Ser Val Glu Lys Trp Asp Val Lys Gly Ser245 250 255Ala Cys Phe Lys Gln Asp Lys Pro Phe Leu Leu Ala Ser Val Ile Glu260 265 270Gly Glu Gly Arg Ile Ala Ser Gly Gly His Asn Phe Ser Phe Lys Lys275 280 285Gly Asp His Met Leu Leu Pro Trp Gly Phe Gly Glu Phe Thr Leu Ala290 295 300Gly His Ala Glu Cys Ile Val Ser Ser Leu Ser Ile Glu305 310 315<210>33<211>1747<212>DNA<213>地衣芽孢桿菌
<220>
<221>CDS<222>(501)..(1244)<400>33tcatacattg ataaaggtga gcttgttccg gatgaagtga caattggtat tgtcagggag 60agacttggca agaatgattg tgacggaggt tttcttctgg acggatttcc gagaacagtc120gctcaagctg aagcacttga ggaaattctt aaaggacttg gcaagtcgat tgaccatgtc180atcaacattc aagtcgataa agatgcattg atggaacgtc tgacaggacg cagaatctgc240aaaaactgcg gtgcaactta tcatttagtc tttaatccgc ctgcaaaaga aaatgtatgc300gacaagtgtg gaggagagct ttatcagcgt gaagacgata atgaagctac cgtatccacg360cggttagagg tcaacatgaa gcaaacccag cctttactcg atttctatga agataaagga420tatctcgtaa acattgacgg gcagaagcac atcaatgaag tttacgctga tataaaggag480cttctcggag gccgggacaa atg att att tgt aaa act ccc cga gaa atc gaa533Met Ile Ile Cys Lys Thr Pro Arg Glu Ile Glu1 5 10atc atg cgg gag gcc gga cga att gtc gct tta act cat cag gag cta 581Ile Met Arg Glu Ala Gly Arg Ile Val Ala Leu Thr His Gln Glu Leu15 20 25aaa aag cac att aaa ccc ggc atc tcg aca aaa gaa ttg gat caa att 629Lys Lys His Ile Lys Pro Gly Ile Ser Thr Lys Glu Leu Asp Gln Ile30 35 40gcc gaa cgt ttt att aca aag caa ggt gca atc cca tct ttt aaa ggg 677Ala Glu Arg Phe Ile Thr Lys Gln Gly Ala Ile Pro Ser Phe Lys Gly45 50 55tat aat ggt ttt cgc ggg agc att tgc gtt tcg gtc aac gaa gaa ctc 725Tyr Asn Gly Phe Arg Gly Ser Ile Cys Val Ser Val Asn Glu Glu Leu60 65 70 75gtg cac ggt att ccg gga aaa cga gtc ctt cgc gac ggg gac atc atc 773Val His Gly Ile Pro Gly Lys Arg Val Leu Arg Asp Gly Asp Ile Ile80 85 90agc atc gat atc ggt gcg aag cta aac ggt tat cat ggt gac tcc gct 821Ser Ile Asp Ile Gly Ala Lys Leu Asn Gly Tyr His Gly Asp Ser Ala95 100 105tgg act tat cca gtg ggt acg atc agc gat gat gat cag aag ctg ctg 869Trp Thr Tyr Pro Val Gly Thr Ile Ser Asp Asp Asp Gln Lys Leu Leu110 115 120gat gtg aca gag gag tct tta tat aga ggc ttg aag gaa gca aaa ccg 917Asp Val Thr Glu Glu Ser Leu Tyr Arg Gly Leu Lys Glu Ala Lys Pro125 130 135gga gaa cgt ttg tcg aat att tcc cac gca ata caa acg tat gtc gaa 965Gly Glu Arg Leu Ser Asn Ile Ser His Ala Ile Gln Thr Tyr Val Glu140 145 150 155agc gaa caa ttt tca gtc gtc agg gaa tat gta gga cac ggg gta ggg 1013Ser Glu Gln Phe Ser Val Val Arg Glu Tyr Val Gly His Gly Val Gly160 165 170cag gat ctg cat gag gat ccg caa att cct cac tac ggt ccg ccg aac 1061Gln Asp Leu His Glu Asp Pro Gln Ile Pro His Tyr Gly Pro Pro Asn175 180 185
aaa ggc ccg cgt tta aag cct ggc atg gtc ttg gcc att gag ccg atg1109Lys Gly Pro Arg Leu Lys Pro Gly Met Val Leu Ala Ile Glu Pro Met190 195 200gtc aat gca ggt aca cgc tat gtc aaa aca ttg gct gac aac tgg acg1157Val Asn Ala Gly Thr Arg Tyr Val Lys Thr Leu Ala Asp Asn Trp Thr205 210 215gtt gta act gtg gac ggg aag aag tgt gct cac ttt gaa cac acg att1205Val Val Thr Val Asp Gly Lys Lys Cys Ala His Phe Glu His Thr Ile220 225 230 235gcc att acg gat gcc ggt ttt gag ata ctg aca aaa gcc taggtgaaat 1254Ala Ile Thr Asp Ala Gly Phe Glu Ile Leu Thr Lys Ala240 245gagatgtatc ggaaaacacc tgaaattgga cagttcgtac gcatgacaag aggccgcgaa 1314atgaaccaat acgcagttgt tattgatata gtcgatcatc atcatgtgct ggtagcagac 1374ggagaaaagc gtaaatttca ttctcctaag aaaaagaata tcaatcattt aaccttttat 1434gattgcgtat ctccggaagt tcagaacagt ataaacgaaa cagggcgtgt gacaaacgga 1494aaattgagat tttctctttt gaaatttgtc agagagcaag ttactgattt gaagaaggga 1554gaacacttga atggcgaaag acgatgtaat tgaagtggaa ggtactgtag tcgaaacgct 1614gccaaacgca atgttcaaag ttgaacttga gaatggccac acggttttgg ctcacgtatc 1674tggtaaaatc cgcatgcact tcattcgcat tttacctgga gacaaagtta cggtagaatt 1734atctccatat gac 1747<210>34<211>248<212>PRT<213>地衣芽孢桿菌<400>34Met Ile Ile Cys Lys Thr Pro Arg Glu Ile Glu Ile Met Arg Glu Ala1 5 10 15Gly Arg Ile Val Ala Leu Thr His Gln Glu Leu Lys Lys His Ile Lys20 25 30Pro Gly Ile Ser Thr Lys Glu Leu Asp Gln Ile Ala Glu Arg Phe Ile35 40 45Thr Lys Gln Gly Ala Ile Pro Ser Phe Lys Gly Tyr Asn Gly Phe Arg50 55 60Gly Ser Ile Cys Val Ser Val Asn Glu Glu Leu Val His Gly Ile Pro65 70 75 80Gly Lys Arg Val Leu Arg Asp Gly Asp Ile Ile Ser Ile Asp Ile Gly85 90 95Ala Lys Leu Asn Gly Tyr His Gly Asp Ser Ala Trp Thr Tyr Pro Val100 105 110
Gly Thr Ile Ser Asp Asp Asp Gln Lys Leu Leu Asp Val Thr Glu Glu115 120 125Ser Leu Tyr Arg Gly Leu Lys Glu Ala Lys Pro Gly Glu Arg Leu Ser130 135 140Asn Ile Ser His Ala Ile Gln Thr Tyr Val Glu Ser Glu Gln Phe Ser145 150 155 160Val Val Arg Glu Tyr Val Gly His Gly Val Gly Gln Asp Leu His Glu165 170 175Asp Pro Gln Ile Pro His Tyr Gly Pro Pro Asn Lys Gly Pro Arg Leu180 185 190Lys Pro Gly Met Val Leu Ala Ile Glu Pro Met Val Asn Ala Gly Thr195 200 205Arg Tyr Val Lys Thr Leu Ala Asp Asn Trp Thr Val Val Thr Val Asp210 215 220Gly Lys Lys Cys Ala His Phe Glu His Thr Ile Ala Ile Thr Asp Ala225 230 235 240Gly Phe Glu Ile Leu Thr Lys Ala245<210>35<211>1928<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1427)<400>35atcaccggct tgaaaccggc cgaagatctc gagctcaaaa cgggagaaac cgtgaaaatc 60gagtttgaaa gcgccgctga tttggatgct gtctttgtga tcagaatgcc gctgaccaat 120ttcaaaaccg ctgcccaaaa cgtaacggag ctgccgatca gagaagtctc aaaaggaaaa 180tatgaaggat attggaccgc tacttcaact gcaaaagcaa aaggagcgga aatcgaggtc 240atcgtcagag atgattacgg caatgaaacg agacaaacgg caaaaggcaa gctgtatatc 300aatgaaaagc tgaaataaag gtgaaaagac gctgtcttta atggcagcgt ttttttcgtt 360ttacgatcga caaattcagt acgaaaactt caaaaaatgt acgatttacg caacattaat 420tgacagactt tacctttggg cttgatttat acttaggaaa acaaacacta aggtcaccga 480gccgcagaaa ggggaaggat gtg gaa atc tat tta gat gcg ata tgg ctg tta 533Val Glu Ile Tyr Leu Asp Ala Ile Trp Leu Leu1 5 10
aac ttt tgt ttt gac ttg ctg ctt tta atg atg acc gca ttt att tta 581Asn Phe Cys Phe Asp Leu Leu Leu Leu Met Met Thr Ala Phe Ile Leu15 20 25aag cga agg gtt aaa aag cgg agg ctg atc cta ggg gca ttt gtc gcg629Lys Arg Arg Val Lys Lys Arg Arg Leu Ile Leu Gly Ala Phe Val Ala30 35 40tca agc atc gtt ctg ttt atg ttt aca cct ttt tca ccg tac gtc ctt 677Ser Ser Ile Val Leu Phe Met Phe Thr Pro Phe Ser Pro Tyr Val Leu45 50 55cat cct gcc ggc aaa ctg tcg ttt tcg gtt gtg atc gtt ctt gtg gca 725His Pro Ala Gly Lys Leu Ser Phe Ser Val Val Ile Val Leu Val Ala60 65 70 75ttt ggt ttt aag cgg ttc cgg ttt ttt ttg cag aat ttg ttt tct ttt 773Phe Gly Phe Lys Arg Phe Arg Phe Phe Leu Gln Asn Leu Phe Ser Phe80 85 90tat ttt gcc act ttt tta atg gga gga ggg att atc gga gcg cat tct 821Tyr Phe Ala Thr Phe Leu Met Gly Gly Gly Ile Ile Gly Ala His Ser95 100 105ttg ctt gaa acg gat tcg atc atg gaa aac ggc gtc ttt atg acg aat 869Leu Leu Glu Thr Asp Ser Ile Met Glu Asn Gly Val Phe Met Thr Asn110 115 120tgg tcc ggt ttt gga gac ccc gtc agc tgg ctg ttt gtc tgt gtg ggt 917Trp Ser Gly Phe Gly Asp Pro Val Ser Trp Leu Phe Val Cys Val Gly125 130 135ttt gcg gct gta tgg ctg ttt tca aaa aag cgt ttt gaa gat gct gaa 965Phe Ala Ala Val Trp Leu Phe Ser Lys Lys Arg Phe Glu Asp Ala Glu140 145 150 155gcg aag aaa att caa tac gaa gaa cgc gtc cgc cta gag gcc tgc att1013Ala Lys Lys Ile Gln Tyr Glu Glu Arg Val Arg Leu Glu Ala Cys Ile160 165 170ggt gaa cat acg ctt cat ttc acc gga ttg att gac tcc gga aac cag1061Gly Glu His Thr Leu His Phe Thr Gly Leu Ile Asp Ser Gly Asn Gln175 180 185ctc tac gat cca atc aca aaa acg ccc gtc atg atc gtc aat att gaa1109Leu Tyr Asp Pro Ile Thr Lys Thr Pro Val Met Ile Val Asn Ile Glu190 195 200aaa ttg aaa gtt gta ttg gga gaa gag gca agt gtg acc atc aag gaa1157Lys Leu Lys Val Val Leu Gly Glu Glu Ala Ser Val Thr Ile Lys Glu205 210 215atg agc ccg ctt gat gcc gtc ggg aaa ctg gat gaa gca ctg ccg tat1205Met Ser Pro Leu Asp Ala Val Gly Lys Leu Asp Glu Ala Leu Pro Tyr220 225 230 235atc ggg cgg atc cgc ctg att ccg tac cgc ggg gtc ggc cat cag cat1253Ile Gly Arg Ile Arg Leu Ile Pro Tyr Arg Gly Val Gly His Gln His240 245 250cag ttt ctg ctc tgc tta aag ccg gat cat gtg ctc gtt tgt acg gaa1301Gln Phe Leu Leu Cys Leu Lys Pro Asp His Val Leu Val Cys Thr Glu255 260 265aga gaa gtg att gaa gcg ccg aaa tgc ctg att ggc atc agc aca tca1349Arg Glu Val Ile Glu Ala Pro Lys Cys Leu Ile Gly Ile Ser Thr Ser270 275 280
ccg ctt tcc gct gac ggc gaa ttt gac gcc atc gtc cat ccg aaa atg1397Pro Leu Ser Ala Asp Gly Glu Phe Asp Ala Ile Val His Pro Lys Met285 290 295ctg gcc gga aac ccg gtc aaa cac gtt tct taaacttgaa gtctgttaca 1447Leu Ala Gly Asn Pro Val Lys His Val Ser300 305ttatcatact cctgaagacg tttatttaga agggggagga agatgaaaaa actaaaatta 1507aggttaacct atctatggta caaactttta atgaaactgg ggctgaaaag cgacgaaatt 1567tattatatcg gcggaagcga ggcgcttccc ccgccattgt caaaagatga agagcaggtg 1627cttctccata agctgcctga cggtgatcag gcggcacgag cgattttgat tgaacgaaat 1687ctcagactgg tcgtgtacat cgcgagaaaa tttgaaaata caggaatcaa tatcgaggat 1747ttaatctcca tcggcacgat cgggctcatc aaagcggtga atacgtttaa tcccgagaaa 1807aaaatcaaac tggctacata tgcttccaga tgcattgaaa atgaaatttt gatgtattta 1867agaagaaaca ataaaatccg ttcagaggta tcattcgacg aaccgctgaa catcgattgg 1927g 1928<210>36<211>309<212>PRT<213>地衣芽孢桿菌<400>36Val Glu Ile Tyr Leu Asp Ala Ile Trp Leu Leu Asn Phe Cys Phe Asp1 5 10 15Leu Leu Leu Leu Met Met Thr Ala Phe Ile Leu Lys Arg Arg Val Lys20 25 30Lys Arg Arg Leu Ile Leu Gly Ala Phe Val Ala Ser Ser Ile Val Leu35 40 45Phe Met Phe Thr Pro Phe Ser Pro Tyr Val Leu His Pro Ala Gly Lys50 55 60Leu Ser Phe Ser Val Val Ile Val Leu Val Ala Phe Gly Phe Lys Arg65 70 75 80Phe Arg Phe Phe Leu Gln Asn Leu Phe Ser Phe Tyr Phe Ala Thr Phe85 90 95Leu Met Gly Gly Gly Ile Ile Gly Ala His Ser Leu Leu Glu Thr Asp100 105 110Ser Ile Met Glu Asn Gly Val Phe Met Thr Asn Trp Ser Gly Phe Gly115 120 125Asp Pro Val Ser Trp Leu Phe Val Cys Val Gly Phe Ala Ala Val Trp130 135 140
Leu Phe Ser Lys Lys Arg Phe Glu Asp Ala Glu Ala Lys Lys Ile Gln145 150 155 160Tyr Glu Glu Arg Val Arg Leu Glu Ala Cys Ile Gly Glu His Thr Leu165 170 175His Phe Thr Gly Leu Ile Asp Ser Gly Asn Gln Leu Tyr Asp Pro Ile180 185 190Thr Lys Thr Pro Val Met Ile Val Asn Ile Glu Lys Leu Lys Val Val195 200 205Leu Gly Glu Glu Ala Ser Val Thr Ile Lys Glu Met Ser Pro Leu Asp210 215 220Ala Val Gly Lys Leu Asp Glu Ala Leu Pro Tyr Ile Gly Arg Ile Arg225 230 235 240Leu Ile Pro Tyr Arg Gly Val Gly His Gln His Gln Phe Leu Leu Cys245 250 255Leu Lys Pro Asp His Val Leu Val Cys Thr Glu Arg Glu Val Ile Glu260 265 270Ala Pro Lys Cys Leu Ile Gly Ile Ser Thr Ser Pro Leu Ser Ala Asp275 280 285Gly Glu Phe Asp Ala Ile Val His Pro Lys Met Leu Ala Gly Asn Pro290 295 300Val Lys His Val Ser305<210>37<211>1390<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1310)<400>37gaagaatttg ctcaagccgc cgtcagaaac agcagcctca cgaaagaaga agcgtttcaa60tttctgattc attcattgct gggaacaggc aagctgttaa tggaaaaaga catgacgttt 120gctcaaacgc ttgatcgggt ggcgacaaaa ggcggcataa ccgcagaagg tgcggaagtc 180attcaagatg ccatgccgga agtctttgac gagctgtttg ccagaacgat gaagaaatat 240gaagtcatca aagacgctgt ccgccggcag tgaaccgcgg cattcatcga catatccgga 300tatccgcaag ctgcctgacc aggcagcttt tttatttgca ttcgacaaca ttcatcaaaa 360
ttccaatttt atattaaatt tctaaaatta ccaattgtta ttgcatttct attaaaattc 420taatataatt tgttagaata ttcccaaaat aataaaaaat ttgagacgtt tttaaggggt 480ggttttttgt gaagctgtat atg tct gtt gat atg gag ggg att tcc ggg ctt 533Met Ser Val Asp Met Glu Gly Ile Ser Gly Leu1 5 10ccg gat gat acg ttc gtc gat tca agc aag ccg aac tat gag aga ggc581Pro Asp Asp Thr Phe Val Asp Ser Ser Lys Pro Asn Tyr Glu Arg Gly15 20 25cgg aag atc atg acg gac gag gcc aat cat gtg att gac gaa gcg ttc629Arg Lys Ile Met Thr Asp Glu Ala Asn His Val Ile Asp Glu Ala Phe30 35 40aaa aat gga tgc aga gag gtg att gtt aat gac agc cac tct aaa atg677Lys Asn Gly Cys Arg Glu Val Ile Val Asn Asp Ser His Ser Lys Met45 50 55aac aac ctg tta att gag agg ctc cat cct gaa gcg cgg ctt att tcg725Asn Asn Leu Leu Ile Glu Arg Leu His Pro G1u Ala Arg Leu Ile Ser60 65 70 75gga gat gtc aag ccg ttt tcg atg gtt cag ggg ctt gat gat aca tac773G1y Asp Val Lys Pro Phe Ser Met Val Gln G1y Leu Asp Asp Thr Tyr80 85 90gac gga gcc gtg ttc gtc ggc tac cat gcg aga gcg tcg atg aga ggc821Asp Gly Ala Val Phe Val Gly Tyr His Ala Arg Ala Ser Met Arg Gly95 100 105gtg atg tcg cac agc atg att ttc ggc gtc aga cat ttt tac atc aac869Val Met Ser His Ser Met Ile Phe Gly Val Arg His Phe Tyr Ile Asn110 115 120gat cag gcg gtc ggc gag ctg ggg ttc aac gcc tat gtt gcc ggt tat917Asp Gln Ala Val Gly Glu Leu Gly Phe Asn Ala Tyr Val Ala Gly Tyr125 130 135tac ggc gtc ccg gtc atc atg gct gcc ggt gac agc gag gcg gca gct965Tyr Gly Val Pro Val Ile Met Ala Ala Gly Asp Ser Glu Ala Ala Ala140 145 150 155gag gcg gag gag ctg att ccg aac gta acg aca gca gcc gtc aaa gaa 1013Glu Ala Glu Glu Leu Ile Pro Asn Val Thr Thr Ala Ala Val Lys Glu160 165 170acg gtc tcc cgt tcg gcg gtc aaa tgt ctg acg ccg gag aaa gcg ggg 1061Thr Val Ser Arg Ser Ala Val Lys Cys Leu Thr Pro Glu Lys Ala Gly175 180 185cgg cta tta aga gaa cgg atg gca tat gcg att caa aac cgc ggt caa 1109Arg Leu Leu Arg Glu Arg Met Ala Tyr Ala Ile Gln Asn Arg Gly Gln190 195 200gtc aag ccg ctc aca cct ccg gac agg ccg gtg ctg cgg ata gaa ttt 1157Val Lys Pro Leu Thr Pro Pro Asp Arg Pro Val Leu Arg Ile Glu Phe205 210 215gce aat tac ggc caa gcc gag tgg gcc agt ctg atg ccg ggt gct gaa 1205Ala Asn Tyr Gly Gln Ala Glu Trp Ala Ser Leu Met Pro Gly Ala Glu220 225 230 235atc gaa ccg caa acg acg act gtc cgc tat caa gcg aaa gac att ctt 1253Ile Glu Pro Gln Thr Thr Thr Val Arg Tyr Gln Ala Lys Asp Ile Leu240 245 250gaa gcc tat caa gcg atg ctt gtc atg acc gag ctt gcg atg aga acg 1301
Glu Ala Tyr Gln Ala Met Leu Val Met Thr Glu Leu Ala Met Arg Thr255 260 265aca ttc tcc taggaaggtg ggatgaaaat ggcgggttac atcatgaagc 1350Thr Phe Ser270ggctgctgtg gatgctggca accattatgg tactccgagg 1390<210>38<211>270<212>PRT<213>地衣芽孢桿菌<400>38Met Ser Val Asp Met Glu Gly Ile Ser Gly Leu Pro Asp Asp Thr Phe1 5 10 15Val Asp Ser Ser Lys Pro Asn Tyr Glu Arg Gly Arg Lys Ile Met Thr20 25 30Asp Glu Ala Asn His Val Ile Asp Glu Ala Phe Lys Asn Gly Cys Arg35 40 45Glu Val Ile Val Asn Asp Ser His Ser Lys Met Asn Asn Leu Leu Ile50 55 60Glu Arg Leu His Pro Glu Ala Arg Leu Ile Ser Gly Asp Val Lys Pro65 70 75 80Phe Ser Met Val Gln Gly Leu Asp Asp Thr Tyr Asp Gly Ala Val Phe85 90 95Val Gly Tyr His Ala Arg Ala Ser Met Arg Gly Val Met Ser His Ser100 105 110Met Ile Phe Gly Val Arg His Phe Tyr Ile Asn Asp Gln Ala Val Gly115 120 125Glu Leu Gly Phe Asn Ala Tyr Val Ala Gly Tyr Tyr Gly Val Pro Val130 135 140Ile Met Ala Ala Gly Asp Ser Glu Ala Ala Ala Glu Ala Glu Glu Leu145 150 155 160Ile Pro Asn Val Thr Thr Ala Ala Val Lys Glu Thr Val Ser Arg Ser165 170 175Ala Val Lys Cys Leu Thr Pro Glu Lys Ala Gly Arg Leu Leu Arg Glu180 185 190Arg Met Ala Tyr Ala Ile Gln Asn Arg Gly Gln Val Lys Pro Leu Thr195 200 205
Pro Pro Asp Arg Pro Val Leu Arg Ile Glu Phe Ala Asn Tyr Gly Gln210 215 220Ala Glu Trp Ala Ser Leu Met Pro Gly Ala Glu Ile Glu Pro Gln Thr225 230 235 240Thr Thr Val Arg Tyr Gln Ala Lys Asp Ile Leu Glu Ala Tyr Gln Ala245 250 255Met Leu Val Met Thr Glu Leu Ala Met Arg Thr Thr Phe Ser260 265 270<210>39<211>2119<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(745)..(1974)<400>39gcttctttta aagttaatcc cagtgctgct tttactaggc gttcttcttc gtcctcagtc 60aagctaacga ttgcccgatt ttgcccttga ttcatttcga tcattttctt gagcgtttct 120ttaatttctg caaagctggg caattgaaac tctacaattg tgatgtcctt ttccaattca 180ggaggaagcg ttaatgttgg cgccacaagc acaacatttt tggaaacaag actcttttcc 240aatacagaga caagatcttt cattttacga atcaaacggt attcaggggg gcgattattt 300gcacccaaat aaacataaaa atccttcaat ataaagactg ctggctgatc atatttttca 360atgaacgcca aagcctttaa cgggtctttt gtttcggctg atggaggcat tccattgctt 420tcaaaccctt tggttaaact ccaaacaaat aaggttcttg gtgttctaat caattctgta 480tccgaaacaa tatgactcag ttgatgtatg acacgttctt cttcccatgt agaaatatac 540aggaacggaa agcgggccct gaaataattc gctacttctt ttctaaagtg atgatatcct 600tccatttttc tccctttcta ttgacctatt ttttattatt atactaaatt tttccacaac 660aggaataaaa gatttcaatc ctcccattcg ccttctctaa atgataagat taaaataaaa 720ccaacaattg gagatgaagg aaac atg aag aac aaa ttg att gaa cgc ctc 771Met Lys Asn Lys Leu Ile Glu Arg Leu1 5att tct tat gcg aaa gtg gat acg cag tca aat gaa aac agt cag acg819Ile Ser Tyr Ala Lys Val Asp Thr Gln Ser Asn Glu Asn Ser Gln Thr10 15 20 25acg cct tcc act ccc ggg cag ctg gcg ctt gcc aat atg ctt gtc gaa867Thr Pro Ser Thr Pro Gly Gln Leu Ala Leu Ala Asn Met Leu Val Glu30 35 40gag ctg aaa gag atc ggc atg aag gac gtc aca atc gat gaa aac ggc915Glu Leu Lys Glu Ile Gly Met Lys Asp Val Thr Ile Asp Glu Asn Gly45 50 55tat gtg atg gcg acg ctt cct tcg aat aca gaa aaa gag gtg ccg acg963
Tyr Val Met Ala Thr Leu Pro Ser Asn Thr Glu Lys Glu Val Pro Thr60 65 70atc ggt ttc ttg gct cat gtg gat aca gca aca gat ttt acc gga aag1011Ile Gly Phe Leu Ala His Val Asp Thr Ala Thr Asp Phe Thr Gly Lys75 80 85aac gtc aat ccg cag gtt atc gaa caa tac gac gga aag gat att gtg1059Asn Val Asn Pro Gln Val Ile Glu Gln Tyr Asp Gly Lys Asp Ile Val90 95 100 105ctg aat gaa tcc ctc aat gtc gtc tta tcg ccc aag gaa ttc ccc gag1107Leu Asn Glu Ser Leu Asn Val Val Leu Ser Pro Lys Glu Phe Pro Glu110 115 120cta gca gat tat gcg gga cat acg ttg att aca acc gac gga acg act1155Leu Ala Asp Tyr Ala Gly His Thr Leu Ile Thr Thr Asp Gly Thr Thr125 130 135ttg ctc ggc gcc gac aat aaa gct ggg atc tct gaa atc atg acg gca1203Leu Leu Gly Ala Asp Asn Lys Ala Gly Ile Ser Glu Ile Met Thr Ala140 145 150atg gaa tat tta att gcg cac ccc gaa atc aaa cac ggg aag atc aga1251Met Glu Tyr Leu Ile Ala His Pro Glu Ile Lys His Gly Lys Ile Arg155 160 165gtc gct ttt aca cct gat gaa gag atc ggc aga ggg ccg cac aag ttt1299Val Ala Phe Thr Pro Asp Glu Glu Ile Gly Arg Gly Pro His Lys Phe170 175 180 185gac gtc gag gcg ttc aat gcc aaa ttc gcg tat acg gtt gac ggc gga1347Asp Val Glu Ala Phe Asn Ala Lys Phe Ala Tyr Thr Val Asp Gly Gly190 195 200ccg ctt ggc gaa ctg cag tac gaa agc ttc aat gcc gca gcc gcg aaa1395Pro Leu Gly Glu Leu Gln Tyr Glu Ser Phe Asn Ala Ala Ala Ala Lys205 210 215atc act tgt aaa gga acg aac gtc cac ccc ggc aca gca aaa gga aaa1443Ile Thr Cys Lys Gly Thr Asn Val His Pro Gly Thr Ala Lys Gly Lys220 225 230atg gtc aat gcc gcc aaa atc gcg atg cag ttc cac gcc gca ttg ccg1491Met Val Asn Ala Ala Lys Ile Ala Met Gln Phe His Ala Ala Leu Pro235 240 245gaa aac gaa gct cct gaa ttt aca gaa ggt tat gaa ggc ttt tat cat1539Glu Asn Glu Ala Pro Glu Phe Thr Glu Gly Tyr Glu Gly Phe Tyr His250 255 260 265ctg ctt tca atc aag ggc gat gtt tct gaa acg agc ctc tct tat atc1587Leu Leu Ser Ile Lys Gly Asp Val Ser Glu Thr Ser Leu Ser Tyr Ile270 275 280att aga gat ttt gac aga gac cga ttt aac gag aga aaa gac acc gtc1635Ile Arg Asp Phe Asp Arg Asp Arg Phe Asn Glu Arg Lys Asp Thr Val285 290 295caa aaa atc gca aac aac ctt aaa gcg aaa tac ggc gaa aac agc gtc1683Gln Lys Ile Ala Asn Asn Leu Lys Ala Lys Tyr Gly Glu Asn Ser Val300 305 310aca gtg gac atg aat gat caa tat tac aac atg agg gaa aag atc gaa1731Thr Val Asp Met Asn Asp Gln Tyr Tyr Asn Met Arg Glu Lys Ile Glu315 320 325ccg gtc aaa gag att gtc gac att gcc tat aaa gcg atg aaa aac ctt1779Pro Val Lys Glu Ile Val Asp Ile Ala Tyr Lys Ala Met Lys Asn Leu
330 335 340 345gat att gaa ccg gtc gtt aaa ccg atc cgc ggc ggt aca gac ggc tct1827Asp Ile Glu Pro Val Val Lys Pro Ile Arg Gly Gly Thr Asp Gly Ser350 355 360cag ctc tca tat atg ggg ctt cct tgt ccg aac att ttc aca ggc ggg1875Gln Leu Ser Tyr Met Gly Leu Pro Cys Pro Asn Ile Phe Thr Gly Gly365 370 375gaa aac ttt cac gga aaa tac gag tat att tcc gcc gac aat atg gta1923Glu Asn Phe His Gly Lys Tyr Glu Tyr Ile Ser Ala Asp Asn Met Val380 385 390aaa gcc gcg aat gtc atc gtg gaa att gtg aag ctg ttt gaa gaa aga1971Lys Ala Ala Asn Val Ile Val Glu Ile Val Lys Leu Phe Glu Glu Arg395 400 405gct taaacgaaaa aagcggaaag cggctgcaag acaaagcagc cgcttttttt 2024Ala410tccagatcaa tctttatctg cgctttgctg aaattagaac atgttgcaag gcggtttctt 2084tttggagcgg gataaagcca attaacatct caccg 2119<210>40<211>410<212>PRT<213>地衣芽孢桿菌<400>40Met Lys Asn Lys Leu Ile Glu Arg Leu Ile Ser Tyr Ala Lys Val Asp1 5 10 15Thr Gln Ser Asn Glu Asn Ser Gln Thr Thr Pro Ser Thr Pro Gly Gln20 25 30Leu Ala Leu Ala Asn Met Leu Val Glu Glu Leu Lys Glu Ile Gly Met35 40 45Lys Asp Val Thr Ile Asp Glu Asn Gly Tyr Val Met Ala Thr Leu Pro50 55 60Ser Asn Thr Glu Lys Glu Val Pro Thr Ile Gly Phe Leu Ala His Val65 70 75 80Asp Thr Ala Thr Asp Phe Thr Gly Lys Asn Val Asn Pro Gln Val Ile85 90 95Glu Gln Tyr Asp Gly Lys Asp Ile Val Leu Asn Glu Ser Leu Asn Val100 105 110Val Leu Ser Pro Lys Glu Phe Pro Glu Leu Ala Asp Tyr Ala Gly His115 120 125Thr Leu Ile Thr Thr Asp Gly Thr Thr Leu Leu Gly Ala Asp Asn Lys130 135 140
Ala Gly Ile Ser Glu Ile Met Thr Ala Met Glu Tyr Leu Ile Ala His145 150 155 160Pro Glu Ile Lys His Gly Lys Ile Arg Val Ala Phe Thr Pro Asp Glu165 170 175Glu Ile Gly Arg Gly Pro His Lys Phe Asp Val Glu Ala Phe Asn Ala180 185 190Lys Phe Ala Tyr Thr Val Asp Gly Gly Pro Leu Gly Glu Leu Gln Tyr195 200 205Glu Ser Phe Asn Ala Ala Ala Ala Lys Ile Thr Cys Lys Gly Thr Asn210 215 220Val His Pro Gly Thr Ala Lys Gly Lys Met Val Asn Ala Ala Lys Ile225 230 235 240Ala Met Gln Phe His Ala Ala Leu Pro Glu Asn Glu Ala Pro Glu Phe245 250 255Thr Glu Gly Tyr Glu Gly Phe Tyr His Leu Leu Ser Ile Lys Gly Asp260 265 270Val Ser Glu Thr Ser Leu Ser Tyr Ile Ile Arg Asp Phe Asp Arg Asp275 280 285Arg Phe Asn Glu Arg Lys Asp Thr Val Gln Lys Ile Ala Asn Asn Leu290 295 300Lys Ala Lys Tyr Gly Glu Asn Ser Val Thr Val Asp Met Asn Asp Gln305 310 315 320Tyr Tyr Asn Met Arg Glu Lys Ile Glu Pro Val Lys Glu Ile Val Asp325 330 335Ile Ala Tyr Lys Ala Met Lys Asn Leu Asp Ile Glu Pro Val Val Lys340 345 350Pro Ile Arg Gly Gly Thr Asp Gly Ser Gln Leu Ser Tyr Met Gly Leu355 360 365Pro Cys Pro Asn Ile Phe Thr Gly Gly Glu Asn Phe His Gly Lys Tyr370 375 380Glu Tyr Ile Ser Ala Asp Asn Met Val Lys Ala Ala Asn Val Ile Val385 390 395 400Glu Ile Val Lys Leu Phe Glu Glu Arg Ala405 410
<210>41<211>1782<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(1238)..(1780)<400>41acaggcacca caacgcataa tcacactcag ccgagaccga aaataaacac ctcaccaaca60aacctaacac caacacaccc cacactcaaa ccacccaagg ataacaaaac cgaacgacca 120cgacatcacg caaaaacggc ccccacaagc agacaaaata caaccgcaca tccaaaaaat 180aaacacatcc caaaataaag acacacgaaa agcaccacct gacaacaaga ccacacacac 240aacaccgaga acaacccacc aaaaccggcc ccacaaaaca catcacacac aaccgagacg 300aaaaccgcag caacgtccca aaacaaccac accccgccac gatgacaaca gacgcacaac 360cgcgcataaa gtagaaaaaa agacgatacg ccacagcaaa aaacagacac agcaccctta 420acctcctacc cccgcatgcc atatacacac tactcatccc caccaaaaca ccccagggca 480cacgcccaca ccgcaaccaa tcccatcacg gaagcgaaca aaaaaaaatc ggcgccatac 540aacaccaacg gaccggaaac caagagaccc gctgcgcgca taatatatcg ctgatacata 600tttggaaaac gttgaatgtt ataacgaagt acggggaatg tacgggttta ccggcactta 660taaaggaaag cgcgtttccg ttcaggggac gggcatgggt gtgccgtcta tttcaattta 720tgtcaatgaa ctgatcagaa gctatgatgt gaaaaacctg atccgcgtcg gatcgtgcgg 780tgcgatcaga aaagacgtca atgtaagaga tgtcattctt gcgatgactt catcaactga 840ttcacaaatc aacagagtgg cattcggctc gatcgacttt gcgccctgcg cggacttcgg 900cctgctaaaa gctgcttatg acgctgcaag tgagcggaac atcccggtta cggcagggaa 960tgtgtttacg gcggatcaat tctataacga cgacagccaa attgagaagc ttgccaaaca 1020cggagtgctt gcggtcgaga tggaaacgac ggcgctttat acattggctg cgaaattcgg 1080ccgaaaagcc ctctccattt taacggtcag cgatcacgtc attaccggcg aggaaacaac 1140ggcagaagaa agacagacga cctttaatga tatgattctg ctggctctgg atactgcttt 1200gtaaaagcat acatgcactg aaaaaggatg gattcga atg aac gga aag tac aaa 1255Met Asn Gly Lys Tyr Lys1 5tac gtg acg att gct tca ttg ctg agt gca gcg gtc ctg ctt ggc aca1303Tyr Val Thr Ile Ala Ser Leu Leu Ser Ala Ala Val Leu Leu Gly Thr10 15 20gga tgt acc atg acc gaa cag aag caa aat gac acg gct aaa aca cag1351Gly Cys Thr Met Thr Glu Gln Lys Gln Asn Asp Thr Ala Lys Thr Gln25 30 35aat act gca aat gca agc aag gat gca aaa gac caa aag tca gac agc1399Asn Thr Ala Asn Ala Ser Lys Asp Ala Lys Asp Gln Lys Ser Asp Ser40 45 50agc ctg gaa gat cag gac ttc ata ctc gag agc aag tac ttt aat caa1447
Ser Leu Glu Asp Gln Asp Phe Ile Leu Glu Ser Lys Tyr Phe Asn Gln55 60 65 70ata aaa gat gta gac ggg ctg ccg acg att caa aat ccg gaa aac ata1495Ile Lys Asp Val Asp Gly Leu Pro Thr Ile Gln Asn Pro Glu Asn Ile75 80 85atg gcg ctt gta aat aaa gaa tac gcg ctt ccc ggc aac tat gca ccg1543Met Ala Leu Val Asn Lys Glu Tyr Ala Leu Pro Gly Asn Tyr Ala Pro90 95 100agc gac ctg aca gtg cct gat gtc gcg ttt tct ttt acc gag gat atc1591Ser Asp Leu Thr Val Pro Asp Val Ala Phe Ser Phe Thr Glu Asp Ile105 110 115gac aag cgt tat atc aga aaa gaa gcc gcc aaa gcg ctt gaa gag atg1639Asp Lys Arg Tyr Ile Arg Lys Glu Ala Ala Lys Ala Leu Glu Glu Met120 125 130ttc agc gcc gct aag aaa gaa ggc tat gaa ctc gtt gca gtg tcc ggc1687Phe Ser Ala Ala Lys Lys Glu Gly Tyr Glu Leu Val Ala Val Ser Gly135 140 145 150tac aga tcc tat gac aga caa aaa gcg atc tat aac aac gaa gtc agc1735Tyr Arg Ser Tyr Asp Arg Gln Lys Ala Ile Tyr Asn Asn Glu Val Ser155 160 165caa aag gga gaa gaa aag gcg aaa gaa gct gtg gca tat ccg ggc ca 1782Gln Lys Gly Glu Glu Lys Ala Lys Glu Ala Val Ala Tyr Pro Gly170 175 180<210>42<211>181<212>PRT<213>地衣芽孢桿菌<400>42Met Asn Gly Lys Tyr Lys Tyr Val Thr Ile Ala Ser Leu Leu Ser Ala1 5 10 15Ala Val Leu Leu Gly Thr Gly Cys Thr Met Thr Glu Gln Lys Gln Asn20 25 30Asp Thr Ala Lys Thr Gln Asn Thr Ala Asn Ala Ser Lys Asp Ala Lys35 40 45Asp Gln Lys Ser Asp Ser Ser Leu Glu Asp Gln Asp Phe Ile Leu Glu50 55 60Ser Lys Tyr Phe Asn Gln Ile Lys Asp Val Asp Gly Leu Pro Thr Ile65 70 75 80Gln Asn Pro Glu Asn Ile Met Ala Leu VaI Asn Lys Glu Tyr Ala Leu85 90 95Pro Gly Asn Tyr Ala Pro Ser Asp Leu Thr Val Pro Asp Val Ala Phe100 105 110Ser Phe Thr Glu Asp Ile Asp Lys Arg Tyr Ile Arg Lys Glu Ala Ala115 120 125
Lys Ala Leu Glu Glu Met Phe Ser Ala Ala Lys Lys Glu Gly Tyr Glu130 135 140Leu Val Ala Val Ser Gly Tyr Arg Ser Tyr Asp Arg Gln Lys Ala Ile145 150 155 160Tyr Asn Asn Glu Val Ser Gln Lys Gly Glu Glu Lys Ala Lys Glu Ala165 170 175Val Ala Tyr Pro Gly180<210>43<211>1663<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(3)..(1166)<400>43tc cgg tct gtg gcc atc gga gtt tgg att gga acc gga tca aga cat 47Arg Ser Val Ala Ile Gly Val Trp Ile Gly Thr Gly Ser Arg His1 5 10 15gaa aca ccc gaa att aac ggc att tcg cac ttt ctt gag cac atg ttt 95Glu Thr Pro Glu Ile Asn Gly Ile Ser His Phe Leu Glu His Met Phe20 25 30ttc aag ggg aca aaa acg cgc act gca agg gac atc gca gag tct ttc143Phe Lys Gly Thr Lys Thr Arg Thr Ala Arg Asp Ile Ala Glu Ser Phe35 40 45gat aga atc ggc gga cag gtc aac gcg ttt acc tca aag gaa tat acg191Asp Arg Ile Gly Gly Gln Val Asn Ala Phe Thr Ser Lys Glu Tyr Thr50 55 60tgc tat tac gcc aaa gtg ctc gat gag cat gca agc tat gct ctg gaa239Cys Tyr Tyr Ala Lys Val Leu Asp Glu His Ala Ser Tyr Ala Leu Glu65 70 75gtg ctt tct gac atg ttc ttt cat tca tca ttt gac gaa gaa gaa tta287Val Leu Set Asp Met Phe Phe His Ser Ser Phe Asp Glu Glu Glu Leu80 85 90 95aaa aaa gaa aaa aac gtc gtc tat gaa gag att aaa atg tat gag gac335Lys Lys Glu Lys Asn Val Val Tyr Glu Glu Ile Lys Met Tyr Glu Asp100 105 110aca cct gat gac atc gtc cac gat ctc ttg agc aag gcg tca tac ggc383Thr Pro Asp Asp Ile Val His Asp Leu Leu Ser Lys Ala Ser Tyr Gly115 120 125agc cat tcc ctc ggc tat ccg atc ctc ggc aca gag gaa aca ctt gcc431Ser His Ser Leu Gly Tyr Pro Ile Leu Gly Thr Glu Glu Thr Leu Ala130 135 140gaa ttt gac gga gat tcg ctc aga aaa tat atg aac gaa tat tac acg479Glu Phe Asp Gly Asp Ser Leu Arg Lys Tyr Met Asn Glu Tyr Tyr Thr145 150 155
cct gac cga gtc gtc att tcc ata gcc gga aat gtg ccg gaa act ttt 527Pro Asp Arg Val Val Ile Ser Ile Ala Gly Asn Val Pro Glu Thr Phe160 165 170 175ata aaa gaa gct gaa aag cat ttc ggt tca tat gaa gcc aaa ggc aaa 575Ile Lys Glu Ala Glu Lys His Phe Gly Ser Tyr Glu Ala Lys Gly Lys180 185 190aga acc ggc atg aca aaa ccg gat ttt cac cat gag aaa atg acg cgc 623Arg Thr Gly Met Thr Lys Pro Asp Phe His His Glu Lys Met Thr Arg195 200 205aaa aag gaa acc gaa cag gct cac ctc tgc ctc ggc ttc aac ggg ctg 671Lys Lys Glu Thr Glu Gln Ala His Leu Cys Leu Gly Phe Asn Gly Leu210 215 220gaa gca ggc cat ccg gaa atc tac gat ttg atc gtt ctc aat aat att 719Glu Ala Gly His Pro Glu Ile Tyr Asp Leu Ile Val Leu Asn Asn Ile225 230 235ttg gga gga agc atg agc agc cgc ctc ttt cag gat gtc cgc gaa gat 767Leu Gly Gly Ser Met Ser Ser Arg Leu Phe Gln Asp Val Arg Glu Asp240 245 250 255aaa ggt ctt gca tat tcc gtg ttc agc tac cac act tcc tat gaa gac 815Lys Gly Leu Ala Tyr Ser Val Phe Ser Tyr His Thr Ser Tyr Glu Asp260 265 270agc ggc atg atg acc atc tac gcg gga acc ggc gca aat cag ctt cag 863Ser Gly Met Met Thr Ile Tyr Ala Gly Thr Gly Ala Asn Gln Leu Gln275 280 285ctc ctg tca gaa acg att cat gaa aca ctg cgt gcg ctg aaa agc gac 911Leu Leu Ser Glu Thr Ile His Glu Thr Leu Arg Ala Leu Lys Ser Asp290 295 300ggt att aca ccg aaa gag ctt gaa aac agc aaa gag caa atg aaa ggc 959Gly Ile Thr Pro Lys Glu Leu Glu Asn Ser Lys Glu Gln Met Lys Gly305 310 315agc ctg atg ctc agt ctc gaa agc aca aac agc aaa atg agc cgc aac1007Ser Leu Met Leu Ser Leu Glu Ser Thr Asn Ser Lys Met Ser Arg Asn320 325 330 335ggc aaa aat gag ctt ttg ctc ggt aaa cac cgg acg ctt gat gaa atc1055Gly Lys Asn Glu Leu Leu Leu Gly Lys His Arg Thr Leu Asp Glu Ile340 345 350atc gaa aag ttg aat gcc gtc agc ctc gag cgt gtg aac aat ctt gcc1103Ile Glu Lys Leu Asn Ala Val Ser Leu Glu Arg Val Asn Asn Leu Ala355 360 365aac cgg att ttc acc gat gat tac tca tct gca tta atc agc ccg tcg1151Asn Arg Ile Phe Thr Asp Asp Tyr Ser Ser Ala Leu Ile Ser Pro Ser370 375 380ggt gag ctg ccg aaa taatgcctcc ttaaaacatg cttgtctttt taggcaggca1206Gly Glu Leu Pro Lys385tgttttttgt tatcggttca tacatcataa caaaaggggg tttttttcat gaggctgagt 1266gaactttcgg gaaaagaaat cgttgatgtt aagcgggccg aacggctcgg cgtactgggg 1326cagacggacc ttgagatcaa tgagcaggac ggtcagatta ccgctcttat catcccttct 1386gttaaatggt tcggactagg aagaaaacag gggaatgaca ttaaggtgcc ctggtcgcaa 1446
attcaaaaaa tcggctcgga tatgatcatt ttggatgttc cggaaagcag cgtgacaaaa 1506gaggagtaag caaggtggaa acccgcccgt cccatggggg cgggcttttt gtttttctgg 1566atttcaattc accgctattt cctttttgtc atatgatgaa attagcttat gaattagatc 1626cttgtcaaaa aagaaggtga atgttagagc catgtta 1663<210>44<211>388<212>PRT<213>地衣芽孢桿菌<400>44Arg Ser Val Ala Ile Gly Val Trp Ile Gly Thr Gly Ser Arg His Glu1 5 10 15Thr Pro Glu Ile Asn Gly Ile Ser His Phe Leu Glu His Met Phe Phe20 25 30Lys Gly Thr Lys Thr Arg Thr Ala Arg Asp Ile Ala Glu Ser Phe Asp35 40 45Arg Ile Gly Gly Gln Val Asn Ala Phe Thr Ser Lys Glu Tyr Thr Cys50 55 60Tyr Tyr Ala Lys Val Leu Asp Glu Hi Ala Ser Tyr Ala Leu Glu Val65 70 75 80Leu Ser Asp Met Phe Phe His Ser Ser Phe Asp Glu Glu Glu Leu Lys85 90 95Lys Glu Lys Asn Val Val Tyr Glu Glu Ile Lys Met Tyr Glu Asp Thr100 105 110Pro Asp Asp Ile Val His Asp Leu Leu Ser Lys Ala Ser Tyr Gly Ser115 120 125His Ser Leu Gly Tyr Pro Ile Leu Gly Thr Glu Glu Thr Leu Ala Glu130 135 140Phe Asp Gly Asp Ser Leu Arg Lys Tyr Met Asn Glu Tyr Tyr Thr Pro145 150 155 160Asp Arg Val Val Ile Ser Ile Ala Gly Asn Val Pro Glu Thr Phe Ile165 170 175Lys Glu Ala Glu Lys His Phe Gly Ser Tyr Glu Ala Lys Gly Lys Arg180 185 190Thr Gly Met Thr Lys Pro Asp Phe His His Glu Lys Met Thr Arg Lys195 200 205Lys Glu Thr Glu Gln Ala His Leu Cys Leu Gly Phe Asn Gly Leu Glu
210 215 220Ala Gly His Pro Glu Ile Tyr Asp Leu Ile Val Leu Asn Asn Ile Leu225 230 235 240Gly Gly Ser Met Ser Ser Arg Leu Phe Gln Asp Val Arg Glu Asp Lys245 250 255Gly Leu Ala Tyr Ser Val Phe Ser Tyr His Thr Ser Tyr Glu Asp Ser260 265 270Gly Met Met Thr Ile Tyr Ala Gly Thr Gly Ala Asn Gln Leu Gln Leu275 280 285Leu Ser Glu Thr Ile His Glu Thr Leu Arg Ala Leu Lys Ser Asp Gly290 295 300Ile Thr Pro Lys Glu Leu Glu Asn Ser Lys Glu Gln Met Lys Gly Ser305 310 315 320Leu Met Leu Ser Leu Glu Ser Thr Asn Ser Lys Met Ser Arg Asn Gly325 330 335Lys Asn Glu Leu Leu Leu Gly Lys His Arg Thr Leu Asp Glu Ile Ile340 345 350Glu Lys Leu Asn Ala Val Ser Leu Glu Arg Val Asn Asn Leu Ala Asn355 360 365Arg Ile Phe Thr Asp Asp Tyr Ser Ser Ala Leu Ile Ser Pro Ser Gly370 375 380Glu Leu Pro Lys385<210>45<211>1553<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(499)..(1296)<400>45ctttcaaatc atcccagctg aaatgccccg aatcgttcct ggagacgagg aaggttccgt 60ccgtctgggt aagctgtgca aaattaatca ccggatcgct tgcgccctgg gcatttacgt 120atattgacgt ttccgagccg acaagcgcaa tatcggcgcc gtttgacaga agggcagtca 180tcgttttatc tccgccccag gtcgttttca gttcaacatc aaggccttct tctttaaaaa 240aaccttcaga taaggcgata tataaaggcg tatagaaaac ggaacgggtc acttccgcca 300
gacggatctt ttcggtcttt tgtccgccgc atgaagcgag gataaacagc atcatgatga 360aggataatat ggtaaaaaag aaacgtttca aaccgatttc cctcctttga tttcggttat 420tttcggcata aaatagttta tgttatatac taaacaggtg tgagggacag ctgcggatta 480aaagaggtga cgtgacga atg tgg ata gaa aaa aga agg ttt cca tcg ccg 531Met Trp Ile Glu Lys Arg Arg Phe Pro Ser Pro1 5 10aac aaa gag gtg cgt ctg ttt tcc gtc aca tac ctt tca caa gga ctc 579Asn Lys Glu Val Arg Leu Phe Ser Val Thr Tyr Leu Ser Gln Gly Leu15 20 25aag gtt aag ggg ctt ttg gcc gaa ccg gcc gct ccc gga aat tat gac 627Lys Val Lys Gly Leu Leu Ala Glu Pro Ala Ala Pro Gly Asn Tyr Asp30 35 40ggg ttt ttg tat ttg cgc ggc gga att aaa aac gtc ggg atg gtc aga 675Gly Phe Leu Tyr Leu Arg Gly Gly Ile Lys Asn Val Gly Met Val Arg45 50 55ccg ggc cga atc gtc cag ttc gct tca cag ggc ttt gtc gtt ctt gct 723Pro Gly Arg Ile Val Gln Phe Ala Ser Gln Gly Phe Val Val Leu Ala60 65 70 75ccg ttt tac agg ggc aat caa gga gga gaa ggc gat gag gat ttt gca 771Pro Phe Tyr Arg Gly Asn Gln Gly Gly Glu Gly Asp Glu Asp Phe Ala80 85 90ggt aat gac cgg gaa gat gct ttt gcc gct ttt cgt ctt ctt cag aag 819Gly Asn Asp Arg Glu Asp Ala Phe Ala Ala Phe Arg Leu Leu Gln Lys95 100 105cac gaa ctg gta aag gac gga cgc gtt cac att ttt ggc ttt tcc cgc 867His Glu Leu Val Lys Asp Gly Arg Val His Ile Phe Gly Phe Ser Arg110 115 120ggc gga atc atg ggc atc tta acg gca atc gaa atg aaa gca gcg gcc 915Gly Gly Ile Met Gly Ile Leu Thr Ala Ile Glu Met Lys Ala Ala Ala125 130 135gct tcg ttt gtg tct tgg ggc ggt gtc agc gac atg gtc atg acc tat 963Ala Ser Phe Val Ser Trp Gly Gly Val Ser Asp Met Val Met Thr Tyr140 145 150 155cgc gaa cga aaa gat ttg cgg cgg atg atg aag cgg gtc atc ggc ggg1011Arg Glu Arg Lys Asp Leu Arg Arg Met Met Lys Arg Val Ile Gly Gly160 165 170acg cct gaa aag gtt ccc gaa gcg tat gaa tgg agg acg cct ttt gac1059Thr Pro Glu Lys Val Pro Glu Ala Tyr Glu Trp Arg Thr Pro Phe Asp175 180 185gag ctg gag aag att gaa gcg ccc ggc ttt ttg atc cac gga gca acc1107Glu Leu Glu Lys Ile Glu Ala Pro Gly Phe Leu Ile His Gly Ala Thr190 195 200gtt gaa aat gtc tcc atc gag cat gcg cgg cgc ctt gaa gcg gga ctt1155Val Glu Asn Val Ser Ile Glu His Ala Arg Arg Leu Glu Ala Gly Leu205 210 215ctg cga ggg aca agt ctg ttg gaa act ggg aat ttt agc cca tat acc1203Leu Arg Gly Thr Ser Leu Leu Glu Thr Gly Asn Phe Ser Pro Tyr Thr220 225 230 235cac tat ttt cct ccg cgc gaa aac agg cgg att gtt aga gaa tta tcc1251
His Tyr Phe Pro Pro Arg Glu Asn Arg Arg Ile Val Arg Glu Leu Ser240 245 250caa tgg atg aaa agc cgt cct tgg aca agt tat ggt aaa cta aag1296Gln Trp Met Lys Ser Arg Pro Trp Thr Ser Tyr Gly Lys Leu Lys255 260 265taaaagagaa aaggaggggg actttaggtg ggaatgccag ttgaatttaa cacgatgatt 1356gtgacaaaag gaaaagaaac gcgggttgag gaaaatgtgt ttacactcga gaaagaaggt 1416tatcgggttt accctcttca tgtgccgctt gaagtcagaa agaccaagcg gggcgacatt 1476accggcaccg cgcaggcgga gcgccttgaa tggtcagacg gacggactca tttaacatac 1536aggctcgtat cattaaa 1553<210>46<211>266<212>PRT<213>地衣芽孢桿菌<400>46Met Trp Ile Glu Lys Arg Arg Phe Pro Ser Pro Asn Lys Glu Val Arg1 5 10 15Leu Phe Ser Val Thr Tyr Leu Ser Gln Gly Leu Lys Val Lys Gly Leu20 25 30Leu Ala Glu Pro Ala Ala Pro Gly Asn Tyr Asp Gly Phe Leu Tyr Leu35 40 45Arg Gly Gly Ile Lys Asn Val Gly Met Val Arg Pro Gly Arg Ile Val50 55 60Gln Phe Ala Ser Gln Gly Phe Val Val Leu Ala Pro Phe Tyr Arg Gly65 70 75 80Asn Gln Gly Gly Glu Gly Asp Glu Asp Phe Ala Gly Asn Asp Arg Glu85 90 95Asp Ala Phe Ala Ala Phe Arg Leu Leu Gln Lys His Glu Leu Val Lys100 105 110Asp Gly Arg Val His Ile Phe Gly Phe Ser Arg Gly Gly Ile Met Gly115 120 125Ile Leu Thr Ala Ile Glu Met Lys Ala Ala Ala Ala Ser Phe Val Ser130 135 140Trp Gly Gly Val Ser Asp Met Val Met Thr Tyr Arg Glu Arg Lys Asp145 150 155 160Leu Arg Arg Met Met Lys Arg Val Ile Gly Gly Thr Pro Glu Lys Val165 170 175
Pro Glu Ala Tyr Glu Trp Arg Thr Pro Phe Asp Glu Leu Glu Lys Ile180 185 190Glu Ala Pro Gly Phe Leu Ile His Gly Ala Thr Val Glu Asn Val Ser195 200 205Ile Glu His Ala Arg Arg Leu Glu Ala Gly Leu Leu Arg Gly Thr Ser210 215 220Leu Leu Glu Thr Gly Asn Phe Ser Pro Tyr Thr His Tyr Phe Pro Pro225 230 235 240Arg Glu Asn Arg Arg Ile Val Arg Glu Leu Ser Gln Trp Met Lys Ser245 250 255Arg Pro Trp Thr Ser Tyr Gly Lys Leu Lys260 265<210>47<211>1576<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1073)<400>47acgaagacga aaggaacggt ctgtgcaccg ctgacattgt ttgcagaata taaaaaccgc 60ggcagtcaaa tcgccgactc tgaatggaag tgggagcttc ataaactcgg tcattccaaa 120aagccgctag aggacgggac tgtgatcgat aaaggcgtgt tcaaaaaaga agccgaaagc 180agcatctacc ggatagagtc aaagcgggca gcaaaaggag ggctatacgc ttttaaattg 240atcagacctg aaggccatcc agatgttaaa aagggcaaac ctttcatttg gtcaaatgta 300atggagcttc aagactgcaa tgaagaaaca ccgaagcctc ctaaaaaagc ttcagcaaaa 360ccaaagcaaa ctgaacagac tcaaacaacg caaaagtctg aacaggatat agaggaaaca 420ccaaaggaca gtgaatccgt agataaagaa aatagaaaag aagataattc attagaaagc 480ggggaagcgt catgaaacat gtg atg aaa tgg atc agc aat ttc tta tat gtg 533Val Met Lys Trp Ile Ser Asn Phe Leu Tyr Val1 5 10att att ttc aca atc atc atc gcc gcg gtc atc gtc gtg att tcg aca581Ile Ile Phe Thr Ile Ile Ile Ala Ala Val Ile Val Val Ile Ser Thr15 20 25aag tcg tcc ggc ggg gag ccg cag ctc ttc ggc tat caa ttg aaa acg629Lys Ser Ser Gly Gly Glu Pro Gln Leu Phe Gly Tyr Gln Leu Lys Thr30 35 40gtt tta tcc gga tcg atg gag ccg gag ttt aaa aca ggt tct gtg att677Val Leu Ser Gly Ser Met Glu Pro Glu Phe Lys Thr Gly Ser Val Ile45 50 55gcc gtt caa aaa gtt gaa aat ccc ggg tct ttg aaa aag gga gat atc725Ala Val Gln Lys Val Glu Asn Pro Gly Ser Leu Lys Lys Gly Asp Ile
60 65 70 75att aca ttc atg caa gac gaa aac acc atg gtt acc cac cga att atc 773Ile Thr Phe Met Gln Asp Glu Asn Thr Met Val Thr His Arg Ile Ile80 85 90ggt ata aca aaa aat aaa tca aat ctc atg ttc aag aca aag ggt gac 821Gly Ile Thr Lys Asn Lys Ser Asn Leu Met Phe Lys Thr Lys Gly Asp95 100 105aat aac caa aac cot gat tcc gat ccg gta ctg gcg gaa aat gtt gtc 869Asn Asn Gln Asn Pro Asp Ser Asp Pro Val Leu Ala Glu Asn Val Val110 115 120gct aag tat tcg ggc att acg gtt ccg tat gcc ggg tat ttg ctg gac 917Ala Lys Tyr Ser Gly Ile Thr Val Pro Tyr Ala Gly Tyr Leu Leu Asp125 130 135ttt gca agt aaa ccg atc ggc aca gcc att ttg ctg atc gtg ccg gga 965Phe Ala Ser Lys Pro Ile Gly Thr Ala Ile Leu Leu Ile Val Pro Gly140 145 150 155ctc ttg ttg att ctt tat gca gta att act gta tcc gcg gct tta aga1013Leu Leu Leu Ile Leu Tyr Ala Val Ile Thr Val Ser Ala Ala Leu Arg160 165 170gag att gac caa aaa gct aaa gca att gaa gcc gct gga aaa gat caa1061Glu Ile Asp Gln Lys Ala Lys Ala Ile Glu Ala Ala Gly Lys Asp Gln175 180 185tca gtt tcc atg taatcttcgt tggtcacctg aacattcagg tctccatata1113Ser Val Ser Met190tacagatttt cttaattata aaaggaggag ttatcaatgg gtacaaagaa aaaactagga 1173ttaggcgttg cgtctgctgc gcttggactg gcattagtag gaggaggaac ttgggctgcg 1233tttaacgaca tcgaaacaac tcaagcaact tatgcagcag gtacgcttga cttaaatgcg 1293aaagatacat ctgcaagagt gaacttgtcc aacttaaaac caggcgacaa attcactaaa 1353gatttcgagt tcaaaaatga cggatcactt gcgattaaag aagtgctgat gcaggttggc 1413tacagcaatt tcgttgacgg aaacgcgaaa aacggcggaa aaagcacagc ggaagacttc 1473ctgaaacaat ttaaagtcag cgttctgact gtcggagttg aaggcggtaa cggctatcct 1533aaaaacatca ttttagatga agccaacctt tatgatctgt aca1576<210>48<211>191<212>PRT<213>地衣芽孢桿菌<400>48Val Met Lys Trp Ile Ser Asn Phe Leu Tyr Val Ile Ile Phe Thr Ile1 5 10 15Ile Ile Ala Ala Val Ile Val Val Ile Ser Thr Lys Ser Ser Gly Gly20 25 30Glu Pro Gln Leu Phe Gly Tyr Gln Leu Lys Thr Val Leu Ser Gly Ser35 40 45
Met Glu Pro Glu Phe Lys Thr Gly Ser Val Ile Ala Val Gln Lys Val50 55 60Glu Asn Pro Gly Ser Leu Lys Lys Gly Asp Ile Ile Thr Phe Met Gln65 70 75 80Asp Glu Asn Thr Met Val Thr His Arg Ile Ile Gly Ile Thr Lys Asn85 90 95Lys Ser Asn Leu Met Phe Lys Thr Lys Gly Asp Asn Asn Gln Asn Pro100 105 110Asp Ser Asp Pro Val Leu Ala Glu Asn Val Val Ala Lys Tyr Ser Gly115 120 125Ile Thr Val Pro Tyr Ala Gly Tyr Leu Leu Asp Phe Ala Ser Lys Pro130 135 140Ile Gly Thr Ala Ile Leu Leu Ile Val Pro Gly Leu Leu Leu Ile Leu145 150 155 160Tyr Ala Val Ile Thr Val Ser Ala Ala Leu Arg Glu Ile Asp Gln Lys165 170 175Ala Lys Ala Ile Glu Ala Ala Gly Lys Asp Gln Ser Val Ser Met180 185 190<210>49<211>1514<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1016)<400>49tgcccctgct cgtgtttgtt gttgccaatg taaattcgtt cggtttatgg caggggtttt 60tgttgacgtg ggtcggggca tctttaggtt ctatagcggt ttttcttttt atccgccatt 120tcggccagca aaaagcactc ggttttatcc gcaggcatcc atccgtaaaa aaactgatga 180tctgggtgga aaaacggggc ttcggtccgc tgtttctcct gctttgcttt ccattcacgc 240cttcggcggc ggtaaatgtt gtggcaggac tttcgcgagt cagcctgctg cctttttcat 300tggcggtgct ttgcggcaag ttcgtgatga tttttatggt gagctttgtc ggccatgatc 360tgaaggcgct ttttacccag cctttgcgga cagccgccgt gatcatcgtc atgctgatct 420tatggtacgc cggaaaacgg gttgaacatc gtctgaacgt gagattcagc cagagagaga 480aagactaggg ggacacagaa ttg aaa aag cgt ttt ttg gtc ata gcc ggg gta 533Leu Lys Lys Arg Phe Leu Val Ile Ala Gly Val1 5 10
ctg ctt gtc atc gtc gtc ggg att cag gtc aaa aat gcc gtt ttc att 581Leu Leu Val Ile Val Val Gly Ile Gln Val Lys Asn Ala Val Phe Ile15 20 25gaa tat aaa gta gaa ggc gtc agc atg aat ccg acc ttt cag gag ggc 629Glu Tyr Lys Val Glu Gly Val Ser Met Asn Pro Thr Phe Gln Glu Gly30 35 40aac gag ctt ttg atc aac agg ttc gcc cac cgc ttt aaa acg atc agc 677Asn Glu Leu Leu Ile Asn Arg Phe Ala His Arg Phe Lys Thr Ile Ser45 50 55cga ttt gac atc gtg ctg ttt aaa gga cct gac aaa gat ata ttc att 725Arg Phe Asp Ile Val Leu Phe Lys Gly Pro Asp Lys Asp Ile Phe Ile60 65 70 75aaa agg gtg atc ggg ctt ccg ggc gaa acc ctc agg tat gaa gat gat 773Lys Arg Val Ile Gly Leu Pro Gly Glu Thr Leu Arg Tyr Glu Asp Asp80 85 90cag ctg tat atc aac gaa gaa aag atc aaa gag cct tat ctg gac gac 821Gln Leu Tyr Ile Asn Glu Glu Lys Ile Lys Glu Pro Tyr Leu Asp Asp95 100 105tta aag gcc gtc acc gcc gga ggg gac ttg aca ggg gat ttt aca ctg 869Leu Lys Ala Val Thr Ala Gly Gly Asp Leu Thr Gly Asp Phe Thr Leu110 115 120cag gaa gtg acc gga gag gag aag gtg cct gaa aac gag tac ttc gtc 917Gln Glu Val Thr Gly Glu Glu Lys Val Pro Glu Asn Glu Tyr Phe Val125 130 135ctc ggg gac aac cgg atc cac agc ttt gac agc cgc cat ttc ggc ttt 965Leu Gly Asp Asn Arg Ile His Ser Phe Asp Ser Arg His Phe Gly Phe140 145 150 155gtt tca gaa cgg gac atc gtc ggg att gtg acg gaa aga att gat aag1013Val Ser Glu Arg Asp Ile Val Gly Ile Val Thr Glu Arg Ile Asp Lys160 165 170aag tgat tggaga gtacggggga gagtaagcgg ccgaccaagg aatacgatta1066Lyscgcaaatgac gagcccgaaa tgtcaattag tacaacagca tcaataatga cgcatttgct 1126aaatatgaaa attaaaaggc ccggatgatt ccgggctttt ttccgtacta cttcagctta 1186atggcattga tccgtttttg cagcttcgtt ttttcggcgg atgctttcag cttgcctact 1246gctgattgcg ctgctgattt tgtttttttc gttctctttt gttcggccgt tttgactttg 1306gcctttgctg cagaaacttt tttattatag gctgcagctg cttgcttttt cgcggtgttc 1366agcctttttt gcagatttga tttgtctttt ccggcgggaa gctcattgat cgctttttgc 1426gctttgccga ggtttgagtc tgttgccgct ttttcagctt tggcaacggc ctgctttgcg 1486gtcttcaaga ggcttgaacg gaccgcgt 1514<210>50<211>172<212>PRT<213>地衣芽孢桿菌<400>50
Leu Lys Lys Arg Phe Leu Val Ile Ala Gly Val Leu Leu Val Ile Val1 5 10 15Val Gly Ile Gln Val Lys Asn Ala Val Phe Ile Glu Tyr Lys Val Glu20 25 30Gly Val Ser Met Asn Pro Thr Phe Gln Glu Gly Asn Glu Leu Leu Ile35 40 45Asn Arg Phe Ala His Arg Phe Lys Thr Ile Ser Arg Phe Asp Ile Val50 55 60Leu Phe Lys Gly Pro Asp Lys Asp Ile Phe Ile Lys Arg Val Ile Gly65 70 75 80Leu Pro Gly Glu Thr Leu Arg Tyr Glu Asp Asp Gln Leu Tyr Ile Asn85 90 95Glu Glu Lys Ile Lys Glu Pro Tyr Leu Asp Asp Leu Lys Ala Val Thr100 105 110Ala Gly Gly Asp Leu Thr Gly Asp Phe Thr Leu Gln Glu Val Thr Gly115 120 125Glu Glu Lys Val Pro Glu Asn Glu Tyr Phe Val Leu Gly Asp Asn Arg130 135 140Ile His Ser Phe Asp Ser Arg His Phe Gly Phe Val Ser Glu Arg Asp145 150 155 160Ile Val Gly Ile Val Thr Glu Arg Ile Asp Lys Lys165 170<210>51<211>1081<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(1)..(585)<400>51aaa cag ctg tgg tgc atc ttt tta acc ctc gga gtc aga agt ttc ggc 48Lys Gln Leu Trp Cys Ile Phe Leu Thr Leu Gly Val Arg Ser Phe Gly1 5 10 15ctg aca tgg aac tgg gcc aat acg ttc gca gac ggc gct tta gaa gaa 96Leu Thr Trp Asn Trp Ala Asn Thr Phe Ala Asp Gly Ala Leu Glu Glu20 25 30aga aac gcc ggc ttg act tct tac ggg gaa aga ctg att cgc aag gcg144Arg Asn Ala Gly Leu Thr Ser Tyr Gly Glu Arg Leu Ile Arg Lys Ala35 40 45aac aaa aac cgg gca tgg aca gat ctc tcc cat tta tcc gaa aaa agc192
Asn Lys Asn Arg Ala Trp Thr Asp Leu Ser His Leu Ser Glu Lys Ser50 55 60ttt tgg ggt gct att gaa acg gcg gcg tat ccg atc gcc tct cat tcg 240Phe Trp Gly Ala Ile Glu Thr Ala Ala Tyr Pro Ile Ala Ser His Ser65 70 75 80aat gcc cgc agc ctt tgc cct cat ccg aga aat tta aaa gac gat caa 288Asn Ala Arg Ser Leu Cys Pro His Pro Arg Asn Leu Lys Asp Asp Gln85 90 95atc aaa gcg ctt att gat aag aac ggc gtc atc ggc ttg aca ttt gtc 336Ile Lys Ala Leu Ile Asp Lys Asn Gly Val Ile Gly Leu Thr Phe Val100 105 110cct gaa ttc gtg aga aac gga aaa acg cct gtg atc aaa gat atc ctc 384Pro Glu Phe Val Arg Asn Gly Lys Thr Pro Val Ile Lys Asp Ile Leu115 120 125tcg cat att gac cat gtc tgc tct tta cga ggc gag cgg cat atc gga 432Ser His Ile Asp His Val Cys Ser Leu Arg Gly Glu Arg His Ile Gly130 135 140ttc ggg tct gat ttt gac ggc atc gac cgt gtc att ccg aat ctt gaa 480Phe Gly Ser Asp Phe Asp Gly Ile Asp Arg Val Ile Pro Asn Leu Glu145 150 155 160gcc cac aaa gat tac ggg aat ctg atc gaa gcg ctg cag cgc tca tat 528Ala His Lys Asp Tyr Gly Asn Leu Ile Glu Ala Leu Gln Arg Ser Tyr165 170 175aca cca agt caa gtc gac gga ttt ctc ttt caa aat ttc atc agc cgc 576Thr Pro Ser Gln Val Asp Gly Phe Leu Phe Gln Asn Phe Ile Ser Arg180 185 190atc cca ttt taaaaaaata tcatacattt ttcagagggt gttcatctgt 625Ile Pro Phe195tttctccaga aggacactgg tgtttaccaa gactgattat cgtgctacaa tccttttgta 685aagcgatttt acaaaggggg tctgttcatc ttggacggaa atatgaaagc gcttatcaag 745aagccggggg agccgggcgc cagcttcgag ctcgtcccga ttccgaagat cgacaaacac 805gatgtcctga tccaagtcac agccgcatcg atttgcggaa acgatgtcca tatttataat 865tgggcatgaa tgcgccacac acctccggta aacccgacct aatgtctccg gacaccgaac 925tttctcggcc tacaatcctt ccaatgcacc ccacatccgt ccaacaactc cccccagaac 985ccacctagca ttccccacac cccccccact acccttttcg ccccatcacc acctcccccc 1045cctcccccta atattatccc tcctccatag aaattt1081<210>52<211>195<212>PRT<213>地衣芽孢桿菌<400>52Lys Gln Leu Trp Cys Ile Phe Leu Thr Leu Gly Val Arg Ser Phe Gly1 5 10 15Leu Thr Trp Asn Trp Ala Asn Thr Phe Ala Asp Gly Ala Leu Glu Glu20 25 30
Arg Asn Ala Gly Leu Thr Ser Tyr Gly Glu Arg Leu Ile Arg Lys Ala35 40 45Asn Lys Asn Arg Ala Trp Thr Asp Leu Ser His Leu Ser Glu Lys Ser50 55 60Phe Trp Gly Ala Ile Glu Thr Ala Ala Tyr Pro Ile Ala Ser His Ser65 70 75 80Asn Ala Arg Ser Leu Cys Pro His Pro Arg Asn Leu Lys Asp Asp Gln85 90 95Ile Lys Ala Leu Ile Asp Lys Asn Gly Val Ile Gly Leu Thr Phe Val100 105 110Pro G1u Phe Val Arg Asn Gly Lys Thr Pro Val Ile Lys Asp Ile Leu115 120 125Ser His Ile Asp His Val Cys Ser Leu Arg G1y Glu Arg His Ile Gly130 135 140Phe Gly Ser Asp Phe Asp G1y 11e Asp Arg Val Ile Pro Asn Leu Glu145 150 155 160Ala His Lys Asp Tyr Gly Asn Leu Ile Glu Ala Leu Gln Arg Ser Tyr165 170 175Thr Pro Ser Gln Val Asp Gly Phe Leu Phe Gln Asn Phe Ile Ser Arg180 185 190Ile Pro Phe195<210>53<211>3560<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(3122)<400>53aagaaggaaa agccgaatta aagcttggcc atgccgaaca aattccttat ccggatcgtt60cttttcataa agtattttca gtccacacca tttatttttg gacggatttc aaacaggcat 120taaaagaaat ttaccgtgtt ttacaggttg acggcacgct gtttctcgct gtgcatctgg 180aagggcaaat gaaaaaatcg aaaaaaacaa aaggcttttc cttatactca gaagagcaaa 240tcaagcaact gcttgaggaa agccatttca gagacatcac ggtacatatg aacaaaaatt 300actgctgcat ttctgcggta aaatcatgaa ctttgtatga tcatcccttt caatacggaa 360
gggatttttt atgtttgata gagttgaaac tggatcttaa atatcatatt tttgattttt 420aaagaaaagt attccattaa catagcaaac atggtttaat atcaaagtga acgtttttac 480tatattttcc ggaggtattt atg aac aaa agg atc gtg aaa agt tca att gtt 533Met Asn Lys Arg Ile Val Lys Ser Ser Ile Val1 5 10ttc ttt ctc ctt gcc gca tta att ttt ggc cag ctg cct tta ccg aag 581Phe Phe Leu Leu Ala Ala Leu Ile Phe Gly Gln Leu Pro Leu Pro Lys15 20 25aca atg gca gcg gaa gac agc gtt ccg aat aac gaa acc aca ttg acc 629Thr Met Ala Ala Glu Asp Ser Val Pro Asn Asn Glu Thr Thr Leu Thr30 35 40agc gcg tcc cct gtt gaa gct tcg ttt caa agc gat gac gag gtg cat 677Ser Ala Ser Pro Val Glu Ala Ser Phe Gln Ser Asp Asp Glu Val His45 50 55tgg tat aaa gtc aat cct tca aat cag gaa atc gca aac tat acg cac 725Trp Tyr Lys Val Asn Pro Ser Asn Gln Glu Ile Ala Asn Tyr Thr His60 65 70 75ttc cgc gtc aaa ttg aaa tca gat gca gag ctg aac att tcc gtc tac 773Phe Arg Val Lys Leu Lys Ser Asp Ala Glu Leu Asn Ile Ser Val Tyr80 85 90tcc agc ctg gaa aat gca act ggt cat caa acg ttt gac cga tac aac 821Ser Ser Leu Glu Asn Ala Thr Gly His Gln Thr Phe Asp Arg Tyr Asn95 100 105ggc tac tcc tat gaa aat aat cct gct tta atc gat ttt ccg att gcc 869Gly Tyr Ser Tyr Glu Asn Asn Pro Ala Leu Ile Asp Phe Pro Ile Ala110 115 120tgg aaa ggt cct tac tac ata aaa gta gaa aat cac cat gat gag gaa 917Trp Lys Gly Pro Tyr Tyr Ile Lys Val Glu Asn His His Asp Glu Glu125 130 135aac gaa acc act tca att aca gat att tct tac acc atc agc tat gaa 965Asn Glu Thr Thr Ser Ile Thr Asp Ile Ser Tyr Thr Ile Ser Tyr Glu140 145 150 155ggc gtc acc ctg cct ccg tca atc caa gag gca gaa gaa gag tgt ccg1013Gly Val Thr Leu Pro Pro Ser Ile Gln Glu Ala Glu Glu Glu Cys Pro160 165 170gca gaa tta agc gtt tcc gaa agg gaa aca ggc aag ggc ata tta aaa1061Ala Glu Leu Ser Val Ser Glu Arg Glu Thr Gly Lys Gly Ile Leu Lys175 180 185cag tta aga acg atc agg gat gaa gtt ctt tca aaa act gaa aaa gga1109Gln Leu Arg Thr Ile Arg Asp Glu Val Leu Ser Lys Thr Glu Lys Gly190 195 200aaa gag ctg tct tcc ctt tac tat aaa gca gct cca ttc atc agc gca1157Lys Glu Leu Ser Ser Leu Tyr Tyr Lys Ala Ala Pro Phe Ile Ser Ala205 210 215aaa atg ctc ttt aac aaa tcg atg aga gac agt gtc tac aaa gac ctg1205Lys Met Leu Phe Asn Lys Ser Met Arg Asp Ser Val Tyr Lys Asp Leu220 225 230 235gtg cag ctg aag ccg ctg ttt gca gat gtc gct aaa aac gga caa gtg1253Val Gln Leu Lys Pro Leu Phe Ala Asp Val Ala Lys Asn Gly Gln Val240 245 250agc gca tac tcg att acg aat gat gat caa aaa gcg atc agt cgc ctg1301
Ser Ala Tyr Ser Ile Thr Asn Asp Asp Gln Lys Ala Ile Ser Arg Leu255 260 265tac gaa aca gct cgc gcg tcc gtt ccc gag ccg ttg aaa aaa cag ctg1349Tyr Glu Thr Ala Arg Ala Ser Val Pro Glu Pro Leu Lys Lys Gln Leu270 275 280gat caa gtc gcg aaa gac atc ggc atc gaa caa tta aca ggc agc aaa1397Asp Gln Val Ala Lys Asp Ile Gly Ile Glu Gln Leu Thr Gly Ser Lys285 290 295gta tcg gct gtg ctt gaa aaa gcg ggg atg gcg aca gct tca tca agc1445Val Ser Ala Val Leu Glu Lys Ala Gly Met Ala Thr Ala Ser Ser Ser300 305 310 315gcg ccc gaa aac cgt tac atc gta aaa ttg aaa gaa ggc aaa aaa ccg1493Ala Pro Glu Asn Arg Tyr Ile Val Lys Leu Lys Glu Gly Lys Lys Pro320 325 330gga tct ttc aaa tct aaa gcc caa tca tcc ggc gtc cag gca tta gag1541Gly Ser Phe Lys Ser Lys Ala Gln Ser Ser Gly Val Gln Ala Leu Glu335 340 345ccc ctc ggt aaa agc aaa acg gca ttt aaa gat atg tac gtt gtg gaa1589Pro Leu Gly Lys Ser Lys Thr Ala Phe Lys Asp Met Tyr Val Val Glu350 355 360atg aag gaa agc cgt tct tcc gga ttc aaa gcg gcg gca aag caa tat1637Met Lys Glu Ser Arg Ser Ser Gly Phe Lys Ala Ala Ala Lys Gln Tyr365 370 375cag gcg gca gcc tcc aag atc gcc aag atg cct gaa gtg gaa ttc gtc1685Gln Ala Ala Ala Ser Lys Ile Ala Lys Met Pro Glu Val Glu Phe Val380 385 390 395gaa cag gtt cag caa tat gaa gca ctg tca aga gac acc caa tat cca1733Glu Gln Val Gln Gln Tyr Glu Ala Leu Ser Arg Asp Thr Gln Tyr Pro400 405 410tat caa tgg tcg ctc aaa aat aac ggc aaa aac cgt gct gcg aat gct1781Tyr Gln Trp Ser Leu Lys Asn Asn Gly Lys Asn Arg Ala Ala Asn Ala415 420 425gac ata caa ttt gaa cag ctt cag aag ctg atg aaa ggc aaa aag ctg1829Asp Ile Gln Phe Glu Gln Leu Gln Lys Leu Met Lys Gly Lys Lys Leu430 435 440aaa gat aca gta atc gcc gtc gtt gac aca ggc gtt gat cat acc ctt1877Lys Asp Thr Val Ile Ala Val Val Asp Thr Gly Val Asp His Thr Leu445 450 455gcg gat tta agc ggc agc gtc aaa aaa gac gaa ggc tat aac tat gtc1925Ala Asp Leu Ser Gly Ser Val Lys Lys Asp Glu Gly Tyr Asn Tyr Val460 465 470 475ggc cgc acg gcg gat gcg atg gat gac aat ggc cac ggc aca cac gtg1973Gly Arg Thr Ala Asp Ala Met Asp Asp Asn Gly His Gly Thr His Val480 485 490tca ggc atc att gca gcc gcg caa gac aac cat ttt tcg atg gcg gga2021Ser Gly Ile Ile Ala Ala Ala Gln Asp Asn His Phe Ser Met Ala Gly495 500 505atc aat gct tat gcc aaa att ctg cct gtc aaa gtg ctg gat tct tca2069Ile Asn Ala Tyr Ala Lys Ile Leu Pro Val Lys Val Leu Asp Ser Ser510 515 520ggc agt gga gat acg gaa cag att gca aac ggc atc atc tat gcc gcc2117Gly Ser Gly Asp Thr Glu Gln Ile Ala Asn Gly Ile Ile Tyr Ala Ala
525 530 535gac cac ggt gca aaa gtc atc aat tta agt ctt ggc ggg cca tac agc2165Asp His Gly Ala Lys Val Ile Asn Leu Ser Leu Gly Gly Pro Tyr Ser540 545 550 555cgg gtg atg gaa tat gcg ctt aaa tat gcg gct tct aaa aat gtg acg2213Arg Val Met Glu Tyr Ala Leu Lys Tyr Ala Ala Ser Lys Asn Val Thr560 565 570atc gtt gcc gcc acc gga aat gac gga gta tcg gag att tcc tac cct2261Ile Val Ala Ala Thr Gly Asn Asp Gly Val Ser Glu Ile Ser Tyr Pro575 580 585gca tct tcg aaa tat acg ctt tca gtc ggg gcg acc aat aat ctc gat2309Ala Ser Ser Lys Tyr Thr Leu Ser Val Gly Ala Thr Asn Asn Leu Asp590 595 600ctt gtc tcg gac tac tcc aat tat gga aaa ggt ctc gat atg gtg gcg2357Leu Val Ser Asp Tyr Ser Asn Tyr Gly Lys Gly Leu Asp Met Val Ala605 610 615ccg gga acc gat att cca agc ctc gtt ccg gac ggg aat gtc act tat2405Pro Gly Thr Asp Ile Pro Ser Leu Val Pro Asp Gly Asn Val Thr Tyr620 625 630 635atg agc gga aca tcg atg gcg gcg ccg cac gtg gca gct gca gca gga2453Met Ser Gly Thr Ser Met Ala Ala Pro His Val Ala Ala Ala Ala Gly640 645 650ctt ctt ttg tca cag aat ccg tcc ttg aaa cca aag caa atc gca agc2501Leu Leu Leu Ser Gln Asn Pro Ser Leu Lys Pro Lys Gln Ile Ala Ser655 660 665cta ttg acc gag acg aca gca gat gtg gca ttt gaa gag cag gat aat2549Leu Leu Thr Glu Thr Thr Ala Asp Val Ala Phe Glu Glu Gln Asp Asn670 675 680cca aac ccg gat tat gac ctg gat ata gaa ccg gct gca caa att ccc2597Pro Asn Pro Asp Tyr Asp Leu Asp Ile Glu Pro Ala Ala Gln Ile Pro685 690 695gga tat gac ttc gtc tcc ggg tgg gga agg ctg aat gtt ttt cat gca2645Gly Tyr Asp Phe Val Ser Gly Trp Gly Arg Leu Asn Val Phe His Ala700 705 710 715gcc agc gtt ttt gag ctg aac atg aag gtt cat ccc gtt tta aac cgc2693Ala Ser Val Phe Glu Leu Asn Met Lys Val His Pro Val Leu Asn Arg720 725 730cat acg gca gtg aca ggc aca gcc aaa agc ggt gtg acg gtc aaa atc2741His Thr Ala Val Thr Gly Thr Ala Lys Ser Gly Val Thr Val Lys Ile735 740 745ttg cga ggg aag caa gta ttg ggg acg ggc acg gcc gga aaa tca ggc2789Leu Arg Gly Lys Gln Val Leu Gly Thr Gly Thr Ala Gly Lys Ser Gly750 755 760gcg ttt tca gtg aaa att ccg gcc cag aag gcg ggg caa gtt ctt cat2837Ala Phe Ser Val Lys Ile Pro Ala Gln Lys Ala Gly Gln Val Leu His765 770 775gtc gcg gca tcg ggc cat cag gcg gaa acc tcg ctc aga acc gtc gtg2885Val Ala Ala Ser Gly His Gln Ala Glu Thr Ser Leu Arg Thr Val Val780 785 790 795gaa aaa gcg ccg aaa aac ccg tcc gtc aaa cgc atc acg aac aaa gat2933Glu Lys Ala Pro Lys Asn Pro Ser Val Lys Arg Ile Thr Asn Lys Asp800 805 810
act gcc gta acg ggt aga acg gca gcc ggc tac acg atc aaa gtg aaa2981Thr Ala Val Thr Gly Arg Thr Ala Ala Gly Tyr Thr Ile Lys Val Lys815 820 825aac gcg tgc aaa aaa gtg atc gcg caa ggc aga gcg gat gca tcc gtg3029Asn Ala Cys Lys Lys Val Ile Ala Gln Gly Arg Ala Asp Ala Ser Val830 835 840agc gtt aaa gtg aaa atc aac aag caa aaa gat atg ccg ttt tgt atg3077Ser Val Lys Val Lys Ile Asn Lys Gln Lys Asp Met Pro Phe Cys Met845 850 855tct ccg cat ctg ctg atg acc aca gag aaa gcg gcg atg tca aaa3122Ser Pro His Leu Leu Met Thr Thr Glu Lys Ala Ala Met Ser Lys860 865 870tgacggtggc tgacgtcatc ccgccaggcg ccccgaaagt ttatcaggtt tccgataaaa 3182gtacggtgat tcagggaaaa acagaagcaa acgcgcaagt cagcgcaaaa gctaaaggaa 3242agacgatcgc ttccggcaaa gcgaacggaa aaggtgaata caagctgaaa atcagcaggc 3302aaaaagccgg aaccgtcatc ggtgtaacag caaaggataa agccggaaat gtcagcaaag 3362cgacagccgt cactgtgctt gacaaaaccc cgccgtctgc gccgaaggtg aatccggtga 3422caaacaagag cacggccgtt aaggggaaag cagaagcgaa cgccgccatc atcgtcaaat 3482caggaaagaa aacgatcgga accggcaagg ccgataaaaa aggcgcgttt tttgtcaaaa 3542taaaaaaaca aaaggcaa3560<210>54<211>874<212>PRT<213>地衣芽孢桿菌<400>54Met Asn Lys Arg Ile Val Lys Ser Ser Ile Val Phe Phe Leu Leu Ala15 10 15Ala Leu Ile Phe Gly Gln Leu Pro Leu Pro Lys Thr Met Ala Ala Glu20 25 30Asp Ser Val Pro Asn Asn Glu Thr Thr Leu Thr Ser Ala Ser Pro Val35 40 45Glu Ala Ser Phe Gln Ser Asp Asp Glu Val His Trp Tyr Lys Val Asn50 55 60Pro Ser Asn Gln Glu Ile Ala Asn Tyr Thr His Phe Arg Val Lys Leu65 70 75 80Lys Ser Asp Ala Glu Leu Asn Ile Ser Val Tyr Ser Ser Leu Glu Asn85 90 95Ala Thr Gly His Gln Thr Phe Asp Arg Tyr Asn Gly Tyr Ser Tyr Glu100 105 110
Asn Asn Pro Ala Leu Ile Asp Phe Pro Ile Ala Trp Lys Gly Pro Tyr115 120 125Tyr Ile Lys Val Glu Asn His His Asp Glu Glu Asn Glu Thr Thr Ser130 135 140Ile Thr Asp Ile Ser Tyr Thr Ile Ser Tyr Glu Gly Val Thr Leu Pro145 150 155 160Pro Ser Ile Gln Glu Ala Glu Glu Glu Cys Pro Ala Glu Leu Ser Val165 170 175Ser Glu Arg Glu Thr Gly Lys Gly Ile Leu Lys Gln Leu Arg Thr Ile180 185 190Arg Asp Glu Val Leu Ser Lys Thr Glu Lys Gly Lys Glu Leu Ser Ser195 200 205Leu Tyr Tyr Lys Ala Ala Pro Phe Ile Ser Ala Lys Met Leu Phe Asn210 215 220Lys Ser Met Arg Asp Ser Val Tyr Lys Asp Leu Val Gln Leu Lys Pro225 230 235 240Leu Phe Ala Asp Val Ala Lys Asn Gly Gln Val Ser Ala Tyr Ser Ile245 250 255Thr Asn Asp Asp Gln Lys Ala Ile Ser Arg Leu Tyr Glu Thr Ala Arg260 265 270Ala Ser Val Pro Glu Pro Leu Lys Lys Gln Leu Asp Gln Val Ala Lys275 280 285Asp Ile Gly Ile Glu Gln Leu Thr Gly Ser Lys Val Ser Ala Val Leu290 295 300Glu Lys Ala Gly Met Ala Thr Ala Ser Ser Ser Ala Pro Glu Asn Arg305 310 315 320Tyr Ile Val Lys Leu Lys Glu Gly Lys Lys Pro Gly Ser Phe Lys Ser325 330 335Lys Ala Gln Ser Ser Gly Val Gln Ala Leu Glu Pro Leu Gly Lys Ser340 345 350Lys Thr Ala Phe Lys Asp Met Tyr Val Val Glu Met Lys Glu Ser Arg355 360 365Ser Ser Gly Phe Lys Ala Ala Ala Lys Gln Tyr Gln Ala Ala Ala Ser370 375 380
Lys Ile Ala Lys Met Pro Glu Val Glu Phe Val Glu Gln Val Gln Gln385 390 395 400Tyr Glu Ala Leu Ser Arg Asp Thr Gln Tyr Pro Tyr Gln Trp Ser Leu405 410 415Lys Asn Asn Gly Lys Asn Arg Ala Ala Asn Ala Asp Ile Gln Phe Glu420 425 430Gln Leu Gln Lys Leu Met Lys Gly Lys Lys Leu Lys Asp Thr Val Ile435 440 445Ala Val Val Asp Thr Gly Val Asp His Thr Leu Ala Asp Leu Ser Gly450 455 460Ser Val Lys Lys Asp Glu Gly Tyr Asn Tyr Val Gly Arg Thr Ala Asp465 470 475 480Ala Met Asp Asp Asn Gly His Gly Thr His Val Ser Gly Ile Ile Ala485 490 495Ala Ala Gln Asp Asn His Phe Ser Met Ala Gly Ile Asn Ala Tyr Ala500 505 510Lys Ile Leu Pro Val Lys Val Leu Asp Ser Ser Gly Ser Gly Asp Thr515 520 525Glu Gln Ile Ala Asn Gly Ile Ile Tyr Ala Ala Asp His Gly Ala Lys530 535 540Val Ile Asn Leu Ser Leu Gly Gly Pro Tyr Ser Arg Val Met Glu Tyr545 550 555 560Ala Leu Lys Tyr Ala Ala Ser Lys Asn Val Thr Ile Val Ala Ala Thr565 570 575Gly Asn Asp Gly Val Ser Glu Ile Ser Tyr Pro Ala Ser Ser Lys Tyr580 585 590Thr Leu Ser Val Gly Ala Thr Asn Asn Leu Asp Leu Val Ser Asp Tyr595 600 605Ser Asn Tyr Gly Lys Gly Leu Asp Met Val Ala Pro Gly Thr Asp Ile610 615 620Pro Ser Leu Val Pro Asp Gly Asn Val Thr Tyr Met Ser Gly Thr Ser625 630 635 640Met Ala Ala Pro His Val Ala Ala Ala Ala Gly Leu Leu Leu Ser Gln645 650 655Asn Pro Ser Leu Lys Pro Lys Gln Ile Ala Ser Leu Leu Thr Glu Thr
660 665 570Thr Ala Asp Val Ala Phe Glu Glu Gln Asp Asn Pro Asn Pro Asp Tyr675 680 685Asp Leu Asp Ile Glu Pro Ala Ala Gln Ile Pro Gly Tyr Asp Phe Val690 695 700Ser Gly Trp Gly Arg Leu Asn Val Phe His Ala Ala Ser Val Phe Glu705 710 715 720Leu Asn Met Lys Val His Pro Val Leu Asn Arg His Thr Ala Val Thr725 730 735Gly Thr Ala Lys Ser Gly Val Thr Val Lys Ile Leu Arg Gly Lys Gln740 745 750Val Leu Gly Thr Gly Thr Ala Gly Lys Ser Gly Ala Phe Ser Val Lys755 760 765Ile Pro Ala Gln Lys Ala Gly Gln Val Leu His Val Ala Ala Ser Gly770 775 780His Gln Ala Glu Thr Ser Leu Arg Thr Val Val Glu Lys Ala Pro Lys785 790 795 800Asn Pro Ser Val Lys Arg Ile Thr Asn Lys Asp Thr Ala Val Thr Gly805 810 815Arg Thr Ala Ala Gly Tyr Thr Ile Lys Val Lys Asn Ala Cys Lys Lys820 825 830Val Ile Ala Gln Gly Arg Ala Asp Ala Ser Val Ser Val Lys Val Lys835 840 845Ile Asn Lys Gln Lys Asp Met Pro Phe Cys Met Ser Pro His Leu Leu850 855 860Met Thr Thr Glu Lys Ala Ala Met Ser Lys865 870<210>55<211>1540<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(505)..(1050)<400>55catcagaaaa gcactttctt tttctgcatt tgaaccggtc atcgtcagag ctgcgggttt60
tatccgtttt atccgcttta gctgttttat ctgttttatt cgttttatct gttttattac 120tatccgccga acgccgcgct ttcattgact gtctttcttt ttcccgctca gggctgtatg 180gatcgtaatt gtcatagtac tcttcgtagc tgctcatttt gaggcaatca ccgcctttgg 240gtcatgatat gataatgtat tataaagagg ggcgttttgc ctgtgcatat ataacggttt 300ttcgtgctgt aaataagctg atcaactgac aaaattcaac attgaataaa ggatttttcg 360tttcaaccgc taatattata tgtatcaacc atttttttac ggctgcgcct gccggtgctt 420gcggaaagca ttcagacgga cggcctgata tgatagaagg agcggaatgc cgatgaaacg 480atccggaagt gaaggagagt actt atg aaa aat agt aga aaa aaa gag atc 531Met Lys Asn Ser Arg Lys Lys Glu Ile1 5tta tcg tgg gtg aaa aca ctt gtc atc gcc gca gcg ctt gtg atg gtt 579Leu Ser Trp Val Lys Thr Leu Val Ile Ala Ala Ala Leu Val Met Val10 15 20 25tgc cgc tat ttt ctg ttt acg ccg tca acc gtt ttg gga gat tca atg 627Cys Arg Tyr Phe Leu Phe Thr Pro Ser Thr Val Leu Gly Asp Ser Met30 35 40tat ccg act tta gag gac ggc aac atg gtc atg gtt agt aag atc agc 675Tyr Pro Thr Leu G1u Asp Gly Asn Met Val Met Val Ser Lys Ile Ser45 50 55gat att cag cgc ttt gat aaa att att ttt cac gcg cct gat tcg gat 723Asp Ile Gln Arg Phe Asp Lys Ile Ile Phe His Ala Pro Asp Ser Asp60 65 70gaa aac tat gtg aaa agg gtg atc ggg ctc ccg gga gat acg atc gaa 771G1u Asn Tyr Val Lys Arg Val Ile Gly Leu Pro Gly Asp Thr Ile Glu75 80 85atg aag gat gat gtc ctt tat ata aac gga aaa gca tac gat gaa cct 819Met Lys Asp Asp Val Leu Tyr Ile Asn Gly Lys Ala Tyr Asp Glu Pro90 95 100 105tat tta aag aaa aac aag caa aag ctg acg ctt cat gaa cat tta acc 867Tyr Leu Lys Lys Asn Lys Gln Lys Leu Thr Leu His Glu His Leu Thr110 115 120gat aat ttc aca ttg gag aaa tta aca ggt gaa caa aag gtg ccg gaa 915Asp Asn Phe Thr Leu Glu Lys Leu Thr Gly Glu Gln Lys Val Pro Glu125 130 135gat cac ttg ttc gtg atg ggg gat aac cgt caa aac agc aaa gac agc 963Asp His Leu Phe Val Met Gly Asp Asn Arg Gln Asn Ser Lys Asp Ser140 145 150cgc ttt ttc gga ttt atc aca atg gac tcc gtt gtc ggc aaa gtt gag1011Arg Phe Phe Gly Phe Ile Thr Met Asp Ser Val Val Gly Lys Val Glu155 160 165ttc cgt tat ttc cct ttc aat gag atc ggc gga ata gaa tagaaaacaa 1060Phe Arg Tyr Phe Pro Phe Asn Glu Ile Gly Gly Ile Glu170 175 180aaaggcttcc catgatacct aatgggaagt ctttttgttt gtcgcaaagt caccaaaagt 1120ggtttaaacc aagccgctat gcttgagcac ggcggcaatt ctactattca cttaaattta 1180aattagagcc agtttttgat atcgcagttg cagaattagg tctctctgaa ttataaggat 1240tgcctttaat ttggtataaa agcctgttta atcgctgatt acccaaacaa aaataactaa 1300
aaaatcctcg atttaacaat ttagtgtatg gtactcttta aacatgtaat caaaaaacta 1360tctttttcgc tccaaagcaa cataccgtat ttcttggttt gttttttagt tgttctaaat 1420attttttatt gttatcgcaa ttcttacgtt gtttgctgca atgattgaag tactatatta 1480cataaattct gataggaggt cattatgttt aatagacaac agatagatca tctgcttgaa 1540<210>56<211>182<212>PRT<213>地衣芽孢桿菌<400>56Met Lys Asn Ser Arg Lys Lys Glu Ile Leu Ser Trp Val Lys Thr Leu1 5 10 15Val Ile Ala Ala Ala Leu Val Met Val Cys Arg Tyr Phe Leu Phe Thr20 25 30Pro Ser Thr Val Leu Gly Asp Ser Met Tyr Pro Thr Leu Glu Asp Gly35 40 45Asn Met Val Met Val Ser Lys Ile Ser Asp Ile Gln Arg Phe Asp Lys50 55 60Ile Ile Phe His Ala Pro Asp Ser Asp Glu Asn Tyr Val Lys Arg Val65 70 75 80Ile Gly Leu Pro Gly Asp Thr Ile Glu Met Lys Asp Asp Val Leu Tyr85 90 95Ile Asn Gly Lys Ala Tyr Asp Glu Pro Tyr Leu Lys Lys Asn Lys Gln100 105 110Lys Leu Thr Leu His Glu His Leu Thr Asp Asn Phe Thr Leu Glu Lys115 120 125Leu Thr Gly Glu Gln Lys Val Pro Glu Asp His Leu Phe Val Met Gly130 135 140Asp Asn Arg Gln Asn Ser Lys Asp Ser Arg Phe Phe Gly Phe Ile Thr145 150 155 160Met Asp Ser Val Val Gly Lys Val Glu Phe Arg Tyr Phe Pro Phe Asn165 170 175Glu Ile Gly Gly Ile Glu180<210>57<211>2212<212>DNA
<213>地衣芽孢桿菌<220>
<221>CDS<222>(2)..(604)<400>57g ttt tca ggc tct gtt aca caa gga atc atc tca gga acc gaa cgc gct 49Phe Ser Gly Ser Val Thr Gln Gly Ile Ile Ser Gly Thr Glu Arg Ala1 5 10 15gtg ccg gtc gat tcc aac gga gac gga cag ccg gac tgg aac gcc gaa 97Val Pro Val Asp Ser Asn Gly Asp Gly Gln Pro Asp Trp Asn Ala Glu20 25 30gta ctg cag acg gat gct gcc att aac ccg ggt aac agc gga ggc ggg145Val Leu Gln Thr Asp Ala Ala Ile Asn Pro Gly Asn Ser Gly Gly Gly35 40 45ctg ttt aat att gat gga aaa gtc atc ggc att aat tcg atg aaa atc193Leu Phe Asn Ile Asp Gly Lys Val Ile Gly Ile Asn Ser Met Lys Ile50 55 60gct gaa tca gcc gtc gaa ggc atc ggt ctg agc att ccg gcc aac ctt241Ala Glu Ser Ala Val Glu Gly Ile Gly Leu Ser Ile Pro Ala Asn Leu65 70 75 80gcc att ccg gtt atc gaa gat ttg gaa acg tac ggt gaa gtg aga cgg289Ala Ile Pro Val Ile Glu Asp Leu Glu Thr Tyr Gly Glu Val Arg Arg85 90 95ccg tat ctc gga att gag atg aaa tca ctt ggc gat atc gca agc tac337Pro Tyr Leu Gly Ile Glu Met Lys Ser Leu Gly Asp Ile Ala Ser Tyr100 105 110cac tgg cag gaa acg ctt aaa ctg ccg aaa aac gta aca tca ggc gta385His Trp Gln Glu Thr Leu Lys Leu Pro Lys Asn Val Thr Ser Gly Val115 120 125gtt gtg atg ggt gtt cag ccg gta tct cct gca ggc aga gcc ggt ctg433Val Val Met Gly Val Gln Pro Val Ser Pro Ala Gly Arg Ala Gly Leu130 135 140aaa gag ctc gat gtc atc gtc gaa ttt aac ggt gac cgt gtt tac gat481Lys Glu Leu Asp Val Ile Val Glu Phe Asn Gly Asp Arg Val Tyr Asp145 150 155 160atc gtt gat ctg cgt aaa aag ctt tac acc aaa aac gtc ggc gac aag529Ile Val Asp Leu Arg Lys Lys Leu Tyr Thr Lys Asn Val Gly Asp Lys165 170 175gta aaa atc aaa tac ctg cgg ggc gga aaa gaa aag aca aca gaa gta577Val Lys Ile Lys Tyr Leu Arg Gly Gly Lys Glu Lys Thr Thr Glu Val180 185 190aag ctg acc cga tcc caa tta gga agc tgataaagac agtctggccg 624Lys Leu Thr Arg Ser Gln Leu Gly Ser195 200gtacggccgg gctgtttttt tattatgaag cgcccaacga atgatcgtca aaaggaaaaa 684atgtgatttt tggagagtga aatgagattt tttctgagtg aataatcgaa tttttgggtg 744tgaattccaa gtttttgccc tgcaaaaatt atagtttgta aaaaatggac aaagcatcat 804attgcgtttc aattttgcgt acgatatgat gactttaata ttctgacaaa tggccgattt 864
aatgcaggat ttaaagatca gggcggaaag ggagatagga tgagtcagca tgcacttgta 924ttacaatctg attttggcct tgatgacggg gcggtaaatg cgatgtacgg tgtggcttat 984tcggttgaca gttcaattcg gatttttgat ttgacgcaca acattcccgt ttttcacatt 1044tgtgaagctt cctatcggct gctgcagtcc gtttcttatt ggcctgaagg cactgtcttc 1104gtgtcggtcg tcgatccagg ggtcggctca gaacgccgca gcgtcgcagt cagaacctct 1164tccaatcaat atatcatcac accagacaac ggaacactca cccacatcag ccggcaaaat 1224ggaattgccg aggcgcgcta tcttgatgaa gcgcaaaaca ggcttccaaa atcaggcgaa 1284tcacatacat tccatggacg cgacatttac gcctacaccg gggcaagact ggcagccggc 1344gctctcagct tcgacaggat cggtccggaa gtttctatag attccatcgt caaactgccg 1404gtcatggaag cgtacatcga aaatgattgg ataaccggaa cgatcgatat tttagatgtc 1464cggtttggaa acctttggac gaatatcagc cggacgctct tcaagtcact tgatgttcaa 1524tacggagatc cagtagaagt cgtcatcaag catgaagaca caaaagtcta tcagcaaacg 1584ttggcatttg gccggtcgtt tgccgatatg cctgtaggca agccggttgt ttatatcaat 1644tcattagacc gcttgggcat tgccctcaac caaggatcat ttgctaaaac ataccatatt 1704ggcacaggca tcaactggcg gatttctctt cgaaaaagta tatagcgtta gacaaaagag 1764cctgattttc cagtcaggct cttgcttttg tgtcattctt atgacataaa aacggttgtc 1824acacattttt cagctaatcg tgaagacagc aacctcttca aaaaatggtt cttttgtcac 1884attggtacct aagtcatatt gtctaaaaca agagaaaaga atatattgag ggggtgtgcc 1944gctgctatga aaaaattcat tcaattcatt tgtttggaaa acgagtgtcc ggaggaatga 2004tatgaacgac aatgtgcagg ctgcgttgaa aacgctgtct aatgtaaaga gcagaagtat 2064cttgtccgtt tttgtgctat gtctgatcac actgggggtt tacataccat actggtttct 2124atccaggaaa aaaagcttcg atttgctgcc ttatcatgat attccctaca ccacgttaaa 2184agctatcata ttttatcatt tattttct 2212<210>58<211>201<212>PRT<213>地衣芽孢桿菌<400>58Phe Ser Gly Ser Val Thr Gln Gly Ile Ile Ser Gly Thr Glu Arg Ala1 5 10 15Val Pro Val Asp Ser Asn Gly Asp Gly Gln Pro Asp Trp Asn Ala Glu20 25 30Val Leu Gln Thr Asp Ala Ala Ile Asn Pro Gly Asn Ser Gly Gly Gly35 40 45Leu Phe Asn Ile Asp Gly Lys Val Ile Gly Ile Asn Ser Met Lys Ile
50 55 60Ala Glu Ser Ala Val Glu Gly Ile Gly Leu Ser Ile Pro Ala Asn Leu65 70 75 80Ala Ile Pro Val Ile Glu Asp Leu Glu Thr Tyr Gly Glu Val Arg Arg85 90 95Pro Tyr Leu Gly Ile Glu Met Lys Ser Leu Gly Asp Ile Ala Ser Tyr100 105 110His Trp Gln Glu Thr Leu Lys Leu Pro Lys Asn Val Thr Ser Gly Val115 120 125Val Val Met Gly Val Gln Pro Val Ser Pro Ala Gly Arg Ala Gly Leu130 135 140Lys Glu Leu Asp Val Ile Val Glu Phe Asn Gly Asp Arg Val Tyr Asp145 150 155 160Ile Val Asp Leu Arg Lys Lys Leu Tyr Thr Lys Asn Val Gly Asp Lys165 170 175Val Lys Ile Lys Tyr Leu Arg Gly Gly Lys Glu Lys Thr Thr Glu Val180 185 190Lys Leu Thr Arg Ser Gln Leu Gly Ser195 200<210>59<211>1519<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1016)<400>59aaaaagctcg aaaggggttt atgatgaagg atattctgcg tgaaattgga atgattgccc 60gagcattgga ttctataagc aatatcgaat ttaaagaaca tgacctgaca aaagggcaat 120atttgtacct tgtccgaata tgcgaaaacc cgggaatcat tcaggagaag ctggctgaga 180tgattaaagt agacaggacc accgcagccc gtgcaataaa aaaacttgaa atgaagggct 240ttattgaacg gagagatgat gaacataaca aaaaaataaa aaaacttttt ccgacggaaa 300agggaaatga ggtatttcct tttataaaaa gagaaaatga ccattcgaat cgtgtggcat 360tagagggatt ttccgagggg gaagcggaga tcatcttcaa tcttcttcaa agagtaagaa 420aaaatgtaga aaaggactgg gaattcgtga aaaaggggaa caagagaaat tattgacacg 480taaaggagcg gcagattgaa atg aca gta aat ata aaa agg tgt acc ctt gaa 533Met Thr Val Asn Ile Lys Arg Cys Thr Leu Glu1 5 10
gac tta cac aag ctt cag gaa att ggt tat gaa aca ttt aat gag aca 581Asp Leu His Lys Leu Gln Glu Ile Gly Tyr Glu Thr Phe Asn Glu Thr15 20 25ttt aag cat cag aat tcg cca gaa aat atg aaa gcc tac ttg gac aag 629Phe Lys His Gln Asn Ser Pro Glu Asn Met Lys Ala Tyr Leu Asp Lys30 35 40gca ttt aac tta aaa caa tta gaa aaa gaa tta tcc aat agc tct tcg 677Ala Phe Asn Leu Lys Gln Leu Glu Lys Glu Leu Ser Asn Ser Ser Ser45 50 55caa ttc ttt ttt gtt tat ttt aac aac gaa atc gct gga tat tta aag 725Gln Phe Phe Phe Val Tyr Phe Asn Asn Glu Ile Ala Gly Tyr Leu Lys60 65 70 75gtc aac acc gat gag gcc cag tct gaa aaa atg ggt gat gaa tca ctt 773Val Asn Thr Asp Glu Ala Gln Ser Glu Lys Met Gly Asp Glu Ser Leu80 85 90gaa atc gag aga att tat atc aag aac aac ttt caa aaa cac ggc ctg 821Glu Ile Glu Arg Ile Tyr Ile Lys Asn Asn Phe Gln Lys His Gly Leu95 100 105ggc aaa tat ctg ttc aat aaa gct gtg gaa att gca aag gaa ctg aat 869Gly Lys Tyr Leu Phe Asn Lys Ala Val Glu Ile Ala Lys Glu Leu Asn110 115 120aaa aag aaa atc tgg ctg ggc gta tgg gaa aaa aac gaa aat gcg att 917Lys Lys Lys Ile Trp Leu Gly Val Trp Glu Lys Asn Glu Asn Ala Ile125 130 135gct ttt tat aag aaa atg ggg ttt gta caa acc ggc gcc cac tct ttt 965Ala Phe Tyr Lys Lys Met Gly Phe Val Gln Thr Gly Ala His Ser Phe140 145 150 155tat atg gga gat gaa gag caa acg gac ttt atc atg gcc aaa aca atc1013Tyr Met Gly Asp Glu Glu Gln Thr Asp Phe Ile Met Ala Lys Thr Ile160 165 170cta taacttttta aaaggaggat gacgatgtat attccaaaat attttaaagt 1066Leucgaaaatgct gatgaaattt tggattttgt ccaaaaaaac tcttttggca cgattgttac 1126gacagaacaa gggaagccaa tcgcgactca tttgccttta gggctcaata aaaaaggcga 1186tgattactat atcactggcc atatcgctta tggaaacccg caggtgggaa ctttggaaac 1246ctgtaaagat gtgcttgtca tgtttcaggg gccgcacgca tatatttctt cttcctggta 1306tgggcatgag gatgttccaa catggaatta tcaagccgtc catatatacg gtaaagcaag 1366cattctagag agagatgaat taatagaaga attaacaatc atgatggaaa aatacgagaa 1426gcatcgggaa aatccaattt tatgggataa cctttctcct caacttttgg agagacaact 1486gaaagcgatt gtcgggttta agattaaggt gga 1519<210>60<211>172<212>PRT<213>地衣芽孢桿菌<400>60
Met Thr Val Asn Ile Lys Arg Cys Thr Leu Glu Asp Leu His Lys Leu1 5 10 15Gln Glu Ile Gly Tyr Glu Thr Phe Asn Glu Thr Phe Lys His Gln Asn20 25 30Ser Pro Glu Asn Met Lys Ala Tyr Leu Asp Lys Ala Phe Asn Leu Lys35 40 45Gln Leu Glu Lys Glu Leu Ser Asn Ser Ser Ser Gln Phe Phe Phe Val50 55 60Tyr Phe Asn Asn Glu Ile Ala Gly Tyr Leu Lys Val Asn Thr Asp Glu65 70 75 80Ala Gln Ser Glu Lys Met Gly Asp Glu Ser Leu Glu Ile Glu Arg Ile85 90 95Tyr Ile Lys Asn Asn Phe Gln Lys His Gly Leu Gly Lys Tyr Leu Phe100 105 110Asn Lys Ala Val Glu Ile Ala Lys Glu Leu Asn Lys Lys Lys Ile Trp115 120 125Leu Gly Val Trp Glu Lys Asn Glu Asn Ala Ile Ala Phe Tyr Lys Lys130 135 140Met Gly Phe Val Gln Thr Gly Ala His Ser Phe Tyr Met Gly Asp Glu145 150 155 160Glu Gln Thr Asp Phe Ile Met Ala Lys Thr Ile Leu165 170<210>61<211>2072<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(2009)<400>61tcgttgacaa agcggttaaa atcgcggaca agccggttga tcactgttgc ttgttctccg60gcatgtcttc tcactccggt tgcatcaaac atcgtaataa cggcaaatat tgcggaaacg 120gcgaacaaag aagaatccat cccgtgatca agagctacac cggtcgagag cgccgtgacg 180gccgctgagt gtgaactcgg cattccgccc gtgcttgtga caagccgcca atcccatttt 240tttgagacga caaagtaaat gggaaccttg ataaactgag cgaaaaagat cgcggcaaag 300ctcgccagca aaggaaagtt tgtgaggatt tccattttgc agaacatcct ttctataaag 360
aaaatagaga tgaattcaga aaagcacgca gttatttgct aaaatatatg atatgaaacc 420ccatcgggaa aaaatgtttt tcctattata acattgttgt tttaatttta ctcactttgc 480tgataaggag ctgtgaacgc atg ttt tat gcc ttt aaa gat ttc gaa aaa aaa 533Met Phe Tyr Ala Phe Lys Asp Phe Glu Lys Lys1 5 10gaa acg ctt tta atc gga ctg ttt aaa aaa agc cgg ctg tac ggt aag581Glu Thr Leu Leu Ile Gly Leu Phe Lys Lys Ser Arg Leu Tyr Gly Lys15 20 25gct gaa gaa atc gac cgc ctt ttg aac ggc cag ctt tct cag ctg ctg629Ala Glu Glu Ile Asp Arg Leu Leu Asn Gly Gln Leu Ser Gln Leu Leu30 35 40aaa gac ggg gat gta tct tcc aaa aaa gcg aaa gtg tct aaa ata ttt677Lys Asp Gly Asp Val Ser Ser Lys Lys Ala Lys Val Ser Lys Ile Phe45 50 55acc cct tcg ctt cag gga gtc aaa cgc att tat atc gtc gga ttg ggc725Thr Pro Ser Leu Gln Gly Val Lys Arg Ile Tyr Ile Val Gly Leu Gly60 65 70 75cgt gaa gcg gaa ttt acc ttc gag gat gcg aag cag tgc ttt gcc gaa773Arg Glu Ala Glu Phe Thr Phe Glu Asp Ala Lys Gln Cys Phe Ala Glu80 85 90gct gtt cag ctg att cac aaa gat cga aag cag gag tta acc gtc atg821Ala Val Gln Leu Ile His Lys Asp Arg Lys Gln Glu Leu Thr Val Met95 100 105ctc gac agc ttc gta tcc gaa gaa gtc cca gct gct gac gcc gca cat869Leu Asp Ser Phe Val Ser Glu Glu Val Pro Ala Ala Asp Ala Ala His110 115 120gca ttg gct gaa tcg tgc atg ctg tca tgc tat gaa gtg cag gat tac917Ala Leu Ala Glu Ser Cys Met Leu Ser Cys Tyr Glu Val Gln Asp Tyr125 130 135aag cac aga tca aat gtg cct gat caa tgt ctg caa agc gtc tat gtt965Lys His Arg Ser Asn Val Pro Asp Gln Cys Leu Gln Ser Val Tyr Val140 145 150 155ttg aca gac cat gac ctg aag gag att cag gcg agt ctg cat gtc gga 1013Leu Thr Asp His Asp Leu Lys Glu Ile Gln Ala Ser Leu His Val Gly160 165 170cag gtg tac gga aat gcg acg aat tcg gcg aga acc ctc gtg aac atg 1061Gln Val Tyr Gly Asn Ala Thr Asn Ser Ala Arg Thr Leu Val Asn Met175 180 185ccg ggg aac atg ctg aca gcg gcg gat ctc gcg tca tat gcc gct gaa 1109Pro Gly Asn Met Leu Thr Ala Ala Asp Leu Ala Ser Tyr Ala Ala Glu190 195 200ttg gcg gcg aaa tac gaa ttt gaa tgt gaa att ctt gaa aag gct gaa 1157Leu Ala Ala Lys Tyr Glu Phe Glu Cys Glu Ile Leu Glu Lys Ala Glu205 210 215atg gaa gag ctc ggg atg ggc ggt ctc ctc gcg gtc aac caa gga tca 1205Met Glu Glu Leu Gly Met Gly Gly Leu Leu Ala Val Asn Gln Gly Ser220 225 230 235gaa gag ccg ccg aaa atg atc gtc ttg aaa tat caa gga aaa gaa aca 1253Glu Glu Pro Pro Lys Met Ile Val Leu Lys Tyr Gln Gly Lys Glu Thr240 245 250tgg gat gac gtc atc ggt tta gtc ggc aaa ggg att acg ttt gat acc 1301
Trp Asp Asp Val Ile Gly Leu Val Gly Lys Gly Ile Thr Phe Asp Thr255 260 265gga ggc tat tcg att aag aca aag agc ggg att gtc ggc atg aag tcc1349Gly Gly Tyr Ser Ile Lys Thr Lys Ser Gly Ile Val Gly Met Lys Ser270 275 280gat atg ggc gga gcc gcc agc gtt ttg gga gcg atg gaa gcg atc ggc1397Asp Met Gly Gly Ala Ala Ser Val Leu Gly Ala Met Glu Ala Ile Gly285 290 295gaa ttg agg ccg gaa caa aac gtg ctt gcc gtg att ccg tcg act gat1445Glu Leu Arg Pro Glu Gln Asn Val Leu Ala Val Ile Pro Ser Thr Asp300 305 310 315aac atg att tcg gga agc gca atg aag ccg gac gac gtc atc gtt tcc1493Asn Met Ile Ser Gly Ser Ala Met Lys Pro Asp Asp Val Ile Val Ser320 325 330ttg agc ggc aag acg att gag atc ttg aat acg gac gcc gaa gga agg1541Leu Ser Gly Lys Thr Ile Glu Ile Leu Asn Thr Asp Ala Glu Gly Arg335 340 345ctg gcg ctc gct gac gga ctg aca tat gcg aag cac cac gga gct tcc1589Leu Ala Leu Ala Asp Gly Leu Thr Tyr Ala Lys His His Gly Ala Ser350 355 360gtg ctg atc gat gtc gcg aca ctg acc gga ggc gtc gtc gta gct ctg1637Val Leu Ile Asp Val Ala Thr Leu Thr Gly Gly Val Val Val Ala Leu365 370 375gga acg gaa acg acg gga gcg atg aca aat cat gat ccg ctt tat cag1685Gly Thr Glu Thr Thr Gly Ala Met Thr Asn His Asp Pro Leu Tyr Gln380 385 390 395cag gtg aga cag gcg gct gaa gaa gcg gga gaa gcg att tgg cag ctt1733Gln Val Arg Gln Ala Ala Glu Glu Ala Gly Glu Ala Ile Trp Gln Leu400 405 410ccg att act gaa aaa gac aaa aaa aga gta aaa aac agc caa atg gcc1781Pro Ile Thr Glu Lys Asp Lys Lys Arg Val Lys Asn Ser Gln Met Ala415 420 425gat ctt aac aat tca ccg ggc agg gaa ggc cat gcg att atg gcc gga1829Asp Leu Asn Asn Ser Pro Gly Arg Glu Gly His Ala Ile Met Ala Gly430 435 440acg ttc ctc ggc gaa ttc gcc gag cag acg cca tgg gtt cac ctt gac1877Thr Phe Leu Gly Glu Phe Ala Glu Gln Thr Pro Trp Val His Leu Asp445 450 455atc gcc gga acg gca acg aca gct caa aac tca tgc ttt gga ccg aaa1925Ile Ala Gly Thr Ala Thr Thr Ala Gln Asn Ser Cys Phe Gly Pro Lys460 465 470 475ggc gga acg ggc gtg atg gtg aga acg ctt gtc acg ttt gtc gag cgg1973Gly Gly Thr Gly Val Met Val Arg Thr Leu Val Thr Phe Val Glu Arg480 485 490gtt tcg gga aat ttt gta gtc ttt cat tcc ctt tgc tagaggggga 2019Val Ser Gly Asn Phe Val Val Phe His Ser Leu Cys495 500tttttttatg gaaaaacgca gatgtctcat tgacgcatta taaacatggt gta 2072<210>62<211>503<212>PRT
<213>地衣芽孢桿菌<400>62Met Phe Tyr Ala Phe Lys Asp Phe Glu Lys Lys Glu Thr Leu Leu Ile1 5 10 15Gly Leu Phe Lys Lys Ser Arg Leu Tyr Gly Lys Ala Glu Glu Ile Asp20 25 30Arg Leu Leu Asn Gly Gln Leu Ser Gln Leu Leu Lys Asp Gly Asp Val35 40 45Ser Ser Lys Lys Ala Lys Val Ser Lys Ile Phe Thr Pro Ser Leu Gln50 55 60Gly Val Lys Arg Ile Tyr Ile Val Gly Leu Gly Arg Glu Ala Glu Phe65 70 75 80Thr Phe Glu Asp Ala Lys Gln Cys Phe Ala Glu Ala Val Gln Leu Ile85 90 95His Lys Asp Arg Lys Gln Glu Leu Thr Val Met Leu Asp Ser Phe Val100 105 110Ser Glu Glu Val Pro Ala Ala Asp Ala Ala His Ala Leu Ala Glu Ser115 120 125Cys Met Leu Ser Cys Tyr Glu Val Gln Asp Tyr Lys His Arg Ser Asn130 135 140Val Pro Asp Gln Cys Leu Gln Ser Val Tyr Val Leu Thr Asp His Asp145 150 155 160Leu Lys Glu Ile Gln Ala Ser Leu His Val Gly Gln Val Tyr Gly Asn165 170 175Ala Thr Asn Ser Ala Arg Thr Leu Val Asn Met Pro Gly Asn Met Leu180 185 190Thr Ala Ala Asp Leu Ala Ser Tyr Ala Ala Glu Leu Ala Ala Lys Tyr195 200 205Glu Phe Glu Cys Glu Ile Leu Glu Lys Ala Glu Met Glu Glu Leu Gly210 215 220Met Gly Gly Leu Leu Ala Val Asn Gln Gly Ser Glu Glu Pro Pro Lys225 230 235 240Met Ile Val Leu Lys Tyr Gln Gly Lys Glu Thr Trp Asp Asp Val Ile245 250 255Gly Leu Val Gly Lys Gly Ile Thr Phe Asp Thr Gly Gly Tyr Ser Ile
260 265 270Lys Thr Lys Ser Gly Ile Val Gly Met Lys Ser Asp Met Gly Gly Ala275 280 285Ala Ser Val Leu Gly Ala Met Glu Ala Ile Gly Glu Leu Arg Pro Glu290 295 300Gln Asn Val Leu Ala Val Ile Pro Ser Thr Asp Asn Met Ile Ser Gly305 310 315 320Ser Ala Met Lys Pro Asp Asp Val Ile Val Ser Leu Ser Gly Lys Thr325 330 335Ile Glu Ile Leu Asn Thr Asp Ala Glu Gly Arg Leu Ala Leu Ala Asp340 345 350Gly Leu Thr Tyr Ala Lys His His Gly Ala Ser Val Leu Ile Asp Val355 360 365Ala Thr Leu Thr Gly Gly Val Val Val Ala Leu Gly Thr Glu Thr Thr370 375 380Gly Ala Met Thr Asn His Asp Pro Leu Tyr Gln Gln Val Arg Gln Ala385 390 395 400Ala Glu Glu Ala Gly Glu Ala Ile Trp Gln Leu Pro Ile Thr Glu Lys405 410 415Asp Lys Lys Arg Val Lys Asn Ser Gln Met Ala Asp Leu Asn Asn Ser420 425 430Pro Gly Arg Glu Gly His Ala Ile Met Ala Gly Thr Phe Leu Gly Glu435 440 445Phe Ala Glu Gln Thr Pro Trp Val His Leu Asp Ile Ala Gly Thr Ala450 455 460Thr Thr Ala Gln Asn Ser Cys Phe Gly Pro Lys Gly Gly Thr Gly Val65 470 475 480Met Val Arg Thr Leu Val Thr Phe Val Glu Arg Val Ser Gly Asn Phe485 490 495Val Val Phe His Ser Leu Cys500<210>63<211>1544<212>DNA<213>地衣芽孢桿菌
<220>
<221>CDS<222>(501)..(1043)<400>63ctacgcgaca ggcatgcggg taagcgaact ttgcagcctg aaggaatctg atttggatct60gtttttagat acggttttgg tccacgggaa aggcaggaag caaaggtata ttccatttgg 120ttcattcgcc cgggaggctt tggatctgta tttgcaaaat ggaagacgca tccttctgtt 180aaaagcgaaa gagccctgcc cttttatttt tttgaatcaa aggggcgggc ccttaacccc 240gaggggggtt cgttacattt tgggagaact ggtcaagaag acttcgggta cattacatat 300acatccgcat atgctccgcc atacatttgc cacccatttg ttaaatgagg gtgcggatct 360gaggagcgtt caggaactgc ttgggcattc aaatctttct tcaacccagg tgtatacgca 420tgtatctaaa gatatgctca gaaagacata tatgtctcat caccctcggg cacataaagg 480gaaataaagg aggttctttt atg tca tct ttt cac gca acg acg ata ttt gcc 533Met Ser Ser Phe His Ala Thr Thr Ile Phe Ala1 5 10gtc cag cac aac gga aaa agc gcg atg gcg gga gac ggc cag gtt acg 581Val Gln His Asn Gly Lys Ser Ala Met Ala Gly Asp Gly Gln Val Thr15 20 25ttc ggt cag gct gtc gtc atg aag cat acc gca aga aag gta aga aaa 629Phe Gly Gln Ala Val Val Met Lys His Thr Ala Arg Lys Val Arg Lys30 35 40ctc ttc aac gga aag gtc att gcc ggt ttt gcc ggt tct gtt gca gac 677Leu Phe Asn Gly Lys Val Ile Ala Gly Phe Ala Gly Ser Val Ala Asp45 50 55gct ttc acg ctc ttt gaa atg ttt gaa gcg aag ctt gaa gaa tac aac 725Ala Phe Thr Leu Phe Glu Met Phe Glu Ala Lys Leu Glu Glu Tyr Asn60 65 70 75ggc aat ttg cag cgg gcg gca gtc gag ctt gca aaa gag tgg cga agc 773Gly Asn Leu Gln Arg Ala Ala Val Glu Leu Ala Lys Glu Trp Arg Ser80 85 90gat aaa gtc ctc aga aag ctt gag gcg atg ctg atc gtc atg aat gcc 821Asp Lys Val Leu Arg Lys Leu Glu Ala Met Leu Ile Val Met Asn Ala95 100 105gac agc atg ctg ctc gtt tcc gga aca ggc gaa gtg att gaa ccg gac 869Asp Ser Met Leu Leu Val Ser Gly Thr Gly Glu Val Ile Glu Pro Asp110 115 120gac ggc att ttg gcg atc ggc tca ggt gga aac tac gcg ctt gca gcc 917Asp Gly Ile Leu Ala Ile Gly Ser Gly Gly Asn Tyr Ala Leu Ala Ala125 130 135gga agg gcg ctg aaa cgt cat gcc ggc agc cag ctt gac gcg aaa gcg 965Gly Arg Ala Leu Lys Arg His Ala Gly Ser Gln Leu Asp Ala Lys Ala140 145 150 155att gcg agg gct tca ctt gaa acc gcg ggc gaa atc tgt gtg tat acg1013Ile Ala Arg Ala Ser Leu Glu Thr Ala Gly Glu Ile Cys Val Tyr Thr160 165 170aac gat cag atc att gta gaa gaa ctt gaa tagaaaggat atgaggcata 1063Asn Asp Gln Ile Ile Val Glu Glu Leu Glu
175180tggaaaaaaa gccgctgact cctaggcaga tcgtcgaaag gcttgatcaa tacatcgtcg 1123gccagctcga tgcgaaaaaa gcggtcgccg tggcattgag gaaccgctac agaagaagtc 1183ttcttgatga aaaactgcgc gaagaaatcg ttccgaaaaa catattgatg atgggcccga 1243caggtgtggg aaaaaccgag attgcaagac gaatcgcgaa actcgtcggc gctccgttcg 1303tcaaaatcga agcgacgaag ttcacggaag tcggttatgt gggcagagat gtggagtcga 1363tggtcaggga tcttgtggaa acgtcagtcc gcctcgtcaa agaggaaaaa atgaacgaag 1423tgaaaggcat cgctgaagaa aacgcaaata aacgcctcgt ccgcctgctt gtaccgggaa 1483gaaaaaaaca gacaggtgcg aaaaatcctt ttgaaatgct gtttggcgga aatcaggatc 1543a 1544<210>64<211>181<212>PRT<213>地衣芽孢桿菌<400>64Met Ser Ser Phe His Ala Thr Thr Ile Phe Ala Val Gln His Asn Gly1 5 10 15Lys Ser Ala Met Ala Gly Asp Gly Gln Val Thr Phe Gly Gln Ala Val20 25 30Val Met Lys His Thr Ala Arg Lys Val Arg Lys Leu Phe Asn Gly Lys35 40 45Val Ile Ala Gly Phe Ala Gly Ser Val Ala Asp Ala Phe Thr Leu Phe50 55 60Glu Met Phe Glu Ala Lys Leu Glu Glu Tyr Asn Gly Asn Leu Gln Arg65 70 75 80Ala Ala Val Glu Leu Ala Lys Glu Trp Arg Ser Asp Lys Val Leu Arg85 90 95Lys Leu Glu Ala Met Leu Ile Val Met Asn Ala Asp Ser Met Leu Leu100 105 110Val Ser Gly Thr Gly Glu Val Ile Glu Pro Asp Asp Gly Ile Leu Ala115 120 125Ile Gly Ser Gly Gly Asn Tyr Ala Leu Ala Ala Gly Arg Ala Leu Lys130 135 140Arg His Ala Gly Ser Gln Leu Asp Ala Lys Ala Ile Ala Arg Ala Ser145 150 155 160Leu Glu Thr Ala Gly Glu Ile Cys Val Tyr Thr Asn Asp Gln Ile Ile
165170 175Val Glu Glu Leu Glu180<210>65<211>2263<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1763)<400>65aatgaaagag gatgctgaaa aacgcgtgaa atccaacctg acactggaag cgatcgccaa 60agcggaaaac cttcaagtca ctgacgaaga agtcgaagaa gagctttcta aaatggctga 120agcatacaac atgcctgttg aaaacatcaa acaagcaatc ggttctacag acggaatgaa 180agaggactta aaagttcgca aagcaattga ttttcttgta gaaaaccgtt aatatgtttc 240ataatcatac tttatataaa acagggcgcg aatgtttcgt gccttgtttt atacaatttt 300gacttatcat cttttctttg ggaaaggata gataatgtcg ttttgatcaa catacatatt 360gaatatgccg gacggcaaac acaacgttat cgcctttttg gaaagtgtgg taaaatgcaa 420tacataatgg aacgaatgtt ttcaagcgat gtttctttta acgaagaaac aggaatgaca 480aggatataag gggtgaaaga atg ttt aaa ttc aac gag gaa aaa gga caa ttg 533Met Phe Lys Phe Asn Glu Glu Lys Gly Gln Leu1 5 10aaa tgc tcg ttt tgc ggt aaa aca caa gat cag gta cgc aag crt gtc581Lys Cys Ser Phe Cys Gly Lys Thr Gln Asp Gln Val Arg Lys Leu Val15 20 25gcc gga cca ggc gta tat ata tgt gac gag tgc atc gaa ctt tgc act629Ala Gly Pro Gly Val Tyr Ile Cys Asp Glu Cys Ile Glu Leu Cys Thr30 35 40gaa att gta gaa gag gaa ctc ggc tct gaa gaa gaa gtg gag ttc aaa677Glu Ile Val Glu Glu Glu Leu Gly Ser Glu Glu Glu Val Glu Phe Lys45 50 55gat gtt ccg aag cca aag gaa atc cgc gag att ctt gat gaa tac gtc725Asp Val Pro Lys Pro Lys Glu Ile Arg Glu Ile Leu Asp Glu Tyr Val60 65 70 75atc ggc cag gac cag gca aaa aaa tcc ctt gcc gtg gcc gtc tac aat773Ile Gly Gln Asp Gln Ala Lys Lys Ser Leu Ala Val Ala Val Tyr Asn80 85 90cat tac aag cgg atc aat tcg aac agc aaa gtc gac gac gtc gag ctg821His Tyr Lys Arg Ile Asn Ser Asn Ser Lys Val Asp Asp Val Glu Leu95 100 105tca aaa agt aac att tcc atg atc gga ccg act gga agc gga aaa acg869Ser Lys Ser Asn Ile Ser Met Ile Gly Pro Thr Gly Ser Gly Lys Thr110 115 120ctt ctt gct caa acg ctt gcc cgc atc ctg aat gtg ccg ttc gcc att917Leu Leu Ala Gln Thr Leu Ala Arg Ile Leu Asn Val Pro Phe Ala Ile
125 130 135gcg gat gcc acg tcg ctt act gaa gca ggc tat gtc ggt gaa gat gtg 965Ala Asp Ala Thr Ser Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val140 145 150 155gag aac att ttg ctt aag ctg atc cag gca gct gac tac gat gtt gaa1013Glu Asn Ile Leu Leu Lys Leu Ile Gln Ala Ala Asp Tyr Asp Val Glu160 165 170aaa gcg gaa aaa ggc att atc tac atc gat gaa atc gat aaa gtg gcc1061Lys Ala Glu Lys Gly Ile Ile Tyr Ile Asp Glu Ile Asp Lys Val Ala175 180 185aga aag tcc gag aac cct tcg att aca cgc gat gta tca ggc gaa ggc1109Arg Lys Ser Glu Asn Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly190 195 200gtt cag cag gca ttg ctt aaa att ctt gaa ggt acg gtt gcc agc gtt1157Val Gln Gln Ala Leu Leu Lys Ile Leu Glu Gly Thr Val Ala Ser Val205 210 215cct cca caa ggc gga aga aag cat cct cat caa gag ttc atc caa att1205Pro Pro Gln Gly Gly Arg Lys His Pro His Gln Glu Phe Ile Gln Ile220 225 230 235gac aca acc aac atc ctg ttc att tgc ggc gga gct ttt gac gga atc1253Asp Thr Thr Asn Ile Leu Phe Ile Cys Gly Gly Ala Phe Asp Gly Ile240 245 250gag cag atc atc aag cgc cgt ctc ggc cag aag gtc atc ggc ttc ggc1301Glu Gln Ile Ile Lys Arg Arg Leu Gly Gln Lys Val Ile Gly Phe Gly255 260 265tct gac aac aag cat gaa gac ctt gaa aaa gaa gcg ctt ctt tcc aaa1349Ser Asp Asn Lys His Glu Asp Leu Glu Lys Glu Ala Leu Leu Ser Lys270 275 280gtg ctt ccg gaa gac ttg ctt cgc ttc ggg ctg atc cct gaa ttc atc1397Val Leu Pro Glu Asp Leu Leu Arg Phe Gly Leu Ile Pro Glu Phe Ile285 290 295ggc cgt ctc ccg atc atc gca agc ctt gag ccg ctg gat gaa aaa gcg1445Gly Arg Leu Pro Ile Ile Ala Ser Leu Glu Pro Leu Asp Glu Lys Ala300 305 310 315ctg gtt gaa att tta aca aaa ccg aaa aac gcg ctt gtt aaa caa tat1493Leu Val Glu Ile Leu Thr Lys Pro Lys Asn Ala Leu Val Lys Gln Tyr320 325 330aga aaa atg ctt gaa ttg gat gat gtg gag ctt gtg ttt gaa gac gaa1541Arg Lys Met Leu Glu Leu Asp Asp Val Glu Leu Val Phe Glu Asp Glu335 340 345gcg ctc aca gaa atc gct aaa aaa gca att gaa cgt aaa aca gga gca1589Ala Leu Thr Glu Ile Ala Lys Lys Ala Ile Glu Arg Lys Thr Gly Ala350 355 360cgc gga ctt cgt tcc att atc gaa gga atc atg ctc gac gtg atg ttt1637Arg Gly Leu Arg Ser Ile Ile Glu Gly Ile Met Leu Asp Val Met Phe365 370 375gat ctg ccg tct cgc gag gat att gaa aaa tgc gtg atc aca ggc aaa1685Asp Leu Pro Ser Arg Glu Asp Ile Glu Lys Cys Val Ile Thr Gly Lys380 385 390 395acc gtc act gac ggc gaa ccg ccg cgt ctc att atg aaa gac ggc acg1733Thr Val Thr Asp Gly Glu Pro Pro Arg Leu Ile Met Lys Asp Gly Thr400 405 410
gtc gtc aat aaa gac aaa aaa act tca gca taatgcagag aacctcctgg 1783Val Val Asn Lys Asp Lys Lys Thr Ser Ala415 420cctaaaggag gttctttttt tgcatccgga aaccgtcggc gtcccggagc agagtgacgg 1843cttcaaaagc aaaataatcc ctttctgtac gtttattccc tccctgatca ggaaatacta 1903tctgacatac taaagatacg aaaccatttc aggagggacc atcagttgag ctggacgagc 1963attgcacttt ttgtacaact atttttcggg atcatcatcg gattatattt ttggaatttg 2023ctcaaaaatc aaagaaccca aaaggttacg atagacagag agtccaaaaa agagatggaa 2083cagctccgga aaatgagatc aatccattta tcagaacctt tatcggaaaa ggtgaggccg 2143acggctttta aagacatcgt cggccaggag gacggcatta aagcgctgaa agccgccctt 2203tgcggaccga atccgcagca tgtcattatt tacggccctc caggtgtggg aaaaacggca 2263<210>66<211>421<212>PRT<213>地衣芽孢桿菌<400>66Met Phe Lys Phe Asn Glu Glu Lys Gly Gln Leu Lys Cys Ser Phe Cys1 5 10 15Gly Lys Thr Gln Asp Gln Val Arg Lys Leu Val Ala Gly Pro Gly Val20 25 30Tyr Ile Cys Asp Glu Cys Ile Glu Leu Cys Thr Glu Ile Val Glu Glu35 40 45Glu Leu Gly Ser Glu Glu Glu Val Glu Phe Lys Asp Val Pro Lys Pro50 55 60Lys Glu Ile Arg Glu Ile Leu Asp Glu Tyr Val Ile Gly Gln Asp Gln65 70 75 80Ala Lys Lys Ser Leu Ala Val Ala Val Tyr Asn His Tyr Lys Arg Ile85 90 95Asn Ser Asn Ser Lys Val Asp Asp Val Glu Leu Ser Lys Ser Asn Ile100 105 110Ser Met Ile Gly Pro Thr Gly Ser Gly Lys Thr Leu Leu Ala Gln Thr115 120 125Leu Ala Arg Ile Leu Asn Val Pro Phe Ala Ile Ala Asp Ala Thr Ser130 135 140Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu Asn Ile Leu Leu145 150 155 160
Lys Leu Ile Gln Ala Ala Asp Tyr Asp Val Glu Lys Ala Glu Lys Gly165 170 175Ile Ile Tyr Ile Asp Glu Ile Asp Lys Val Ala Arg Lys Ser Glu Asn180 185 190Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val Gln Gln Ala Leu195 200 205Leu Lys Ile Leu Glu Gly Thr Val Ala Ser Val Pro Pro Gln Gly Gly210 215 220Arg Lys His Pro His Gln Glu Phe Ile Gln Ile Asp Thr Thr Asn Ile225 230 235 240Leu Phe Ile Cys Gly Gly Ala Phe Asp Gly Ile Glu Gln Ile Ile Lys245 250 255Arg Arg Leu Gly Gln Lys Val Ile Gly Phe Gly Ser Asp Asn Lys His260 265 270Glu Asp Leu Glu Lys Glu Ala Leu Leu Ser Lys Val Leu Pro Glu Asp275 280 285Leu Leu Arg Phe Gly Leu Ile Pro Glu Phe Ile Gly Arg Leu Pro Ile290 295 300Ile Ala Ser Leu Glu Pro Leu Asp Glu Lys Ala Leu Val Glu Ile Leu305 310 315 320Thr Lys Pro Lys Asn Ala Leu Val Lys Gln Tyr Arg Lys Met Leu Glu325 330 335Leu Asp Asp Val Glu Leu Val Phe Glu Asp Glu Ala Leu Thr Glu Ile340 345 350Ala Lys Lys Ala Ile Glu Arg Lys Thr Gly Ala Arg Gly Leu Arg Ser355 360 365Ile Ile Glu Gly Ile Met Leu Asp Val Met Phe Asp Leu Pro Ser Arg370 375 380Glu Asp Ile Glu Lys Cys Val Ile Thr Gly Lys Thr Val Thr Asp Gly385 390 395 400Glu Pro Pro Arg Leu Ile Met Lys Asp Gly Thr Val Val Asn Lys Asp405 410 415Lys Lys Thr Ser Ala420
<210>67<211>2653<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(2153)<400>67gttgaaattt taacaaaacc gaaaaacgcg cttgttaaac aatatagaaa aatgcttgaa 60ttggatgatg tggagcttgt gtttgaagac gaagcgctca cagaaatcgc taaaaaagca 120attgaacgta aaacaggagc acgcggactt cgttccatta tcgaaggaat catgctcgac 180gtgatgtttg atctgccgtc tcgcgaggat attgaaaaat gcgtgatcac aggcaaaacc 240gtcactgacg gcgaaccgcc gcgtctcatt atgaaagacg gcacggtcgt caataaagac 300aaaaaaactt cagcataatg cagagaacct cctggcctaa aggaggttct ttttttgcat 360ccggaaaccg tcggcatccc ggagcagagt gacggcttca aaagcaaaat aatccctttc 420tgtacgttta ttccctccct gatcaggaaa tactatctga catactaaag atacgaaacc 480atttcaggag ggaccatcag ttg agc tgg acg agc att gca ctt ttt gta caa 533Leu Ser Trp Thr Ser Ile Ala Leu Phe Val Gln1 5 10cta ttt ttc ggg atc atc atc gga tta tat ttt tgg aat ttg ctc aaa581Leu Phe Phe Gly Ile Ile Ile Gly Leu Tyr Phe Trp Asn Leu Leu Lys15 20 25aat caa aga acc caa aag gtt acg ata gac aga gag tcc aaa aaa gag629Asn Gln Arg Thr Gln Lys Val Thr Ile Asp Arg Glu Ser Lys Lys Glu30 35 40atg gaa cag ctc cgg aaa atg aga tca atc cat tta tca gaa cct tta677Met Glu Gln Leu Arg Lys Met Arg Ser Ile His Leu Ser Glu Pro Leu45 50 55tcg gaa aag gtg agg ccg acg gct ttt aaa gac atc gtc ggc cag gag725Ser Glu Lys Val Arg Pro Thr Ala Phe Lys Asp Ile Val Gly Gln Glu60 65 70 75gac ggc att aaa gcg ctg aaa gcc gcc ctt tgc gga ccg aat ccg cag773Asp Gly Ile Lys Ala Leu Lys Ala Ala Leu Cys Gly Pro Asn Pro Gln80 85 90cat gtc att att tac ggc cct cca ggt gtg gga aaa acg gca gct gca821His Val Ile Ile Tyr Gly Pro Pro Gly Val Gly Lys Thr Ala Ala Ala95 100 105agg ctt gtc ctt gaa gaa gcg aaa aag aac aag cgc tea ccg ttc cag869Arg Leu Val Leu Glu Glu Ala Lys Lys Asn Lys Arg Ser Pro Phe Gln110 115 120gaa cat tcc gcc ttt gtc gag ctt gac gcg aca acg gcc cgt ttc gac917Glu His Ser Ala Phe Val Glu Leu Asp Ala Thr Thr Ala Arg Phe Asp125 130 135gag cgg gga atc gcc gac ccg ttg atc ggt tcg gtc cac gat ccg att965Glu Arg Gly Ile Ala Asp Pro Leu Ile Gly Ser Val His Asp Pro Ile140 145 150 155
tat cag ggc gcc gga gcg atg ggg cag gcc gga att ccc cag cca aag1013Tyr Gln Gly Ala Gly Ala Met Gly Gln Ala Gly Ile Pro Gln Pro Lys160 165 170cag gga gcc gtc acg cat gca cac ggt gga gta tta ttc atc gat gaa1061Gln Gly Ala Val Thr His Ala His Gly Gly Val Leu Phe Ile Asp Glu175 180 185atc gga gag ctt cat ccg att caa atg aat aaa atg ctg aag gtg ctc1109Ile Gly Glu Leu His Pro Ile Gln Met Asn Lys Met Leu Lys Val Leu190 195 200gaa gat cgg aaa gtt ttt ttg gaa agc gcc tat tac agt gaa gaa aac1157Glu Asp Arg Lys Val Phe Leu Glu Ser Ala Tyr Tyr Ser Glu Glu Asn205 210 215acg caa atc ccc aag cat att cac gac att ttt caa aac ggg ctg ccg1205Thr Gln Ile Pro Lys His Ile His Asp Ile Phe Gln Asn Gly Leu Pro220 225 230 235gcg gat ttc aga ctg atc ggc gcg acg aca agg atg cct gat gaa atc1253Ala Asp Phe Arg Leu Ile Gly Ala Thr Thr Arg Met Pro Asp Glu Ile240 245 250ccg cct gcc att cgg tcg aga tgt ttg gaa gtg ttc ttc cgc gac ctt1301Pro Pro Ala Ile Arg Ser Arg Cys Leu Glu Val Phe Phe Arg Asp Leu255 260 265gaa aag cac gaa tta aaa acg gtc gcc aaa aaa gcg gca gat aag att1349Glu Lys His Glu Leu Lys Thr Val Ala Lys Lys Ala Ala Asp Lys Ile270 275 280caa aag aaa gtc gca gag gaa gga ctc gac ctg ttg acg cgg tat gcg1397Gln Lys Lys Val Ala Glu Glu Gly Leu Asp Leu Leu Thr Arg Tyr Ala285 290 295aga aac ggc cgc gaa gtc gtc aat atg atg caa atc gcc gcc gga atg1445Arg Asn Gly Arg Glu Val Val Asn Met Met Gln Ile Ala Ala Gly Met300 305 310 315gct ttg act gaa gag cgc gac gag gtg acg gtt gaa gat atc gaa tgg1493Ala Leu Thr Glu Glu Arg Asp Glu Val Thr Val Glu Asp Ile Glu Trp320 325 330gtg atc cat tcg agc cag ctg aca ccg aaa tac gaa caa aaa atc gcc1541Val Ile His Ser Ser Gln Leu Thr Pro Lys Tyr Glu Gln Lys Ile Ala335 340 345tct aaa ccg cag gtc ggc atc gtc aac gga ctt gcc gtc cac ggg cct1589Ser Lys Pro Gln Val Gly Ile Val Asn Gly Leu Ala Val His Gly Pro350 355 360aac agc ggt tcg ctg ctc gaa att gag gtc aca gtc aat cag gcg gct1637Asn Ser Gly Ser Leu Leu Glu Ile Glu Val Thr Val Asn Gln Ala Ala365 370 375gat aaa gga tcg att aat atc aca ggc att gca gaa gaa gag aat atc1685Asp Lys Gly Ser Ile Asn Ile Thr Gly Ile Ala Glu Glu Glu Asn Ile380 385 390 395ggc aac cag tca aaa tcg atc cgc cgg aaa agc atg gcg aaa gga tcg1733Gly Asn Gln Ser Lys Ser Ile Arg Arg Lys Ser Met Ala Lys Gly Ser400 405 410gtc gaa aac gtc atg act gta ttg cgg aca atg gga gtc aaa gct tcg1781Val Glu Asn Val Met Thr Val Leu Arg Thr Met Gly Val Lys Ala Ser415 420 425
gat tat gat att cac gtg aat ttc ccc ggc ggt atc ccg gtt gac gga1829Asp Tyr Asp Ile His Val Asn Phe Pro Gly Gly Ile Pro Val Asp Gly430 435 440cca tct gca ggg att gca atg gcg gcg ggc ata ttc tcc gct att cat1877Pro Ser Ala Gly Ile Ala Met Ala Ala Gly Ile Phe Ser Ala Ile His445 450 455aag att ccg ata gac cat act gtc gcc atg acg ggt gaa atc agc ctg1925Lys Ile Pro Ile Asp His Thr Val Ala Met Thr Gly Glu Ile Ser Leu460465 470 475aac ggc cgg gta aag ccg atc ggc ggc gtg att ccg aaa atc aaa gcg1973Asn Gly Arg Val Lys Pro Ile Gly Gly Val Ile Pro Lys Ile Lys Ala480 485 490gcc aag cat gcg ggg gca aaa aca gtc att att ccg cac gaa aat gtt2021Ala Lys His Ala Gly Ala Lys Thr Val Ile Ile Pro His Glu Asn Val495 500 505cag tcg att tta aag cgg ata gaa ggc ata cat atc gtt ccc gtc aaa2069Gln Ser Ile Leu Lys Arg Ile Glu Gly Ile His Ile Val Pro Val Lys510 515 520acc ttg cag gaa gta ctc gac ctc gta ttg gtc aac cct cct tca gaa2117Thr Leu Gln Glu Val Leu Asp Leu Val Leu Val Asn Pro Pro Ser Glu525 530 535atg ccc gac ggc gtt caa atg aaa aga gaa tcc gtt taaatcccca 2163Met Pro Asp Gly Val Gln Met Lys Arg Glu Ser Val540 545 550tcgtttcaga tggggatttt tttcggggcg gtttctccga agccgggagc cggcatgttt 2223tcttagcgga ggacatcttt tcctttttca gaactgaaag aaagggtata ctacaggaga 2283ccctttttat ttcgccgggc ttacaattgt acaggaataa aaaaagtata ttataatggt 2343tcatactaaa gttacggagg tgtcagtcaa aatggcagat gaaacgaaac gaaacatccc 2403gctcctccct ttaagaggtt tactcgtcta tccgacgatg gttttgcacc ttgacgtcgg 2463gcgtgaaaag tcggttcagg cacttgaaca ggcgatgatg aatgaccata tgatcttttt 2523ggcaacgcaa aaggacatat ccatagacga accggatgaa gacgagattt ttacattcgg 2583aacctatacg aaaatcaagc agatgctcaa gctgccgaac ggaacaatcc gcgttttggt 2643ggaggggttg 2653<210>68<211>551<212>PRT<213>地衣芽孢桿菌<400>68Leu Ser Trp Thr Ser Ile Ala Leu Phe Val Gln Leu Phe Phe Gly Ile1 5 10 15Ile Ile Gly Leu Tyr Phe Trp Asn Leu Leu Lys Asn Gln Arg Thr Gln20 25 30
Lys Val Thr Ile Asp Arg Glu Ser Lys Lys Glu Met Glu Gln Leu Arg35 40 45Lys Met Arg Ser Ile His Leu Ser Glu Pro Leu Ser Glu Lys Val Arg50 55 60Pro Thr Ala Phe Lys Asp Ile Val Gly Gln Glu Asp Gly Ile Lys Ala65 70 75 80Leu Lys Ala Ala Leu Cys Gly Pro Asn Pro Gln His Val Ile Ile Tyr85 90 95Gly Pro Pro Gly Val Gly Lys Thr Ala Ala Ala Arg Leu Val Leu Glu100 105 110Glu Ala Lys Lys Asn Lys Arg Ser Pro Phe Gln Glu His Ser Ala Phe115 120 125Val Glu Leu Asp Ala Thr Thr Ala Arg Phe Asp Glu Arg Gly Ile Ala130 135 140Asp Pro Leu Ile Gly Ser Val His Asp Pro Ile Tyr Gln Gly Ala Gly145 150 155 160Ala Met Gly Gln Ala Gly Ile Pro Gln Pro Lys Gln Gly Ala Val Thr165 170 175His Ala His Gly Gly Val Leu Phe Ile Asp Glu Ile Gly Glu Leu His180 185 190Pro Ile Gln Met Asn Lys Met Leu Lys Val Leu Glu Asp Arg Lys Vall95 200 205Phe Leu Glu Ser Ala Tyr Tyr Ser Glu Glu Asn Thr Gln Ile Pro Lys210 215 220His Ile His Asp Ile Phe Gln Asn Gly Leu Pro Ala Asp Phe Arg Leu225 230 235 240Ile Gly Ala Thr Thr Arg Met Pro Asp Glu Ile Pro Pro Ala Ile Arg245 250 255Ser Arg Cys Leu Glu Val Phe Phe Arg Asp Leu Glu Lys His Glu Leu260 265 270Lys Thr Val Ala Lys Lys Ala Ala Asp Lys Ile Gln Lys Lys Val Ala275 280 285Glu Glu Gly Leu Asp Leu Leu Thr Arg Tyr Ala Arg Asn Gly Arg Glu290 295 300Val Val Asn Met Met Gln Ile Ala Ala Gly Met Ala Leu Thr Glu Glu
305 310 315 320Arg Asp Glu Val Thr Val Glu Asp Ile Glu Trp Val Ile His Ser Ser325 330 335Gln Leu Thr Pro Lys Tyr Glu Gln Lys Ile Ala Ser Lys Pro Gln Val340 345 350Gly Ile Val Asn Gly Leu Ala Val His Gly Pro Asn Ser Gly Ser Leu355 360 365Leu Glu Ile Glu Val Thr Val Asn Gln Ala Ala Asp Lys Gly Ser Ile370 375 380Asn Ile Thr Gly Ile Ala Glu Glu Glu Asn Ile Gly Asn Gln Ser Lys385 390 395 400Ser Ile Arg Arg Lys Ser Met Ala Lys Gly Ser Val Glu Asn Val Met405 410 415Thr Val Leu Arg Thr Met Gly Val Lys Ala Ser Asp Tyr Asp Ile His420 425430Val Asn Phe Pro Gly Gly Ile Pro Val Asp Gly Pro Ser Ala Gly Ile435 440 445Ala Met Ala Ala Gly Ile Phe Ser Ala Ile His Lys Ile Pro Ile Asp450 455 460His Thr Val Ala Met Thr Gly Glu Ile Ser Leu Asn Gly Arg Val Lys465 470 475 480Pro Ile Gly Gly Val Ile Pro Lys Ile Lys Ala Ala Lys His Ala Gly485 490 495Ala Lys Thr Val Ile Ile Pro His Glu Asn Val Gln Ser Ile Leu Lys500 505 510Arg Ile Glu Gly Ile His Ile Val Pro Val Lys Thr Leu Gln Glu Val515 520 525Leu Asp Leu Val Leu Val Asn Pro Pro Ser Glu Met Pro Asp Gly Val530 535 540Gln Met Lys Arg Glu Ser Val545 550<210>69<211>2230<212>DNA<213>地衣芽孢桿菌
<220>
<221>CDS<222>(501)..(1730)<400>69cagagacaag atctttcatt ttacgaatca aacggtattc aggggggcga ttatttgcac60ccaaataaac ataaaaatcc ttcaatataa agactgctgg ctgatcatat ttttcaatga 120acgccaaagc ctttaacggg tcttttgttt cggctgatgg aggcattcca ttgctttcaa 180accctttggt taaactccaa acaaataagg ttcttggtgt tctaatcaat tctgtatccg 240aaacaatatg actcagttga tctatgacac gttcttcttc ccatgtagaa atatacagga 300acggaaagcg ggccctgaaa taattcgcta cttcttttct aaagtgatga tatccttcca 360tttttctccc tttctattga cctatttttt attattatac taaatttttc cacaacagga 420ataaaagattt caatcctcc cattcgcctt ctctaaatga taagattaaa ataaaaccaa 480caattggaga tgaaggaaac atg aag aac aaa ttg att gaa cgc ctc att tct 533Met Lys Asn Lys Leu Ile Glu Arg Leu Ile Ser1 5 10tat gcg aaa gtg gat acg cag tca aat gaa aac agt cag acg acg cct 581Tyr Ala Lys Val Asp Thr Gln Ser Asn Glu Asn Ser Gln Thr Thr Pro15 20 25tcc act ccc ggg cag ctg gcg ctt gcc aat atg ctt gtc gaa gag ctg 629Ser Thr Pro Gly Gln Leu Ala Leu Ala Asn Met Leu Val Glu Glu Leu30 35 40aaa gag atc ggc atg aag gac gtc aca atc gat gaa aac ggc tat gtg 677Lys Glu Ile Gly Met Lys Asp Val Thr Ile Asp Glu Asn Gly Tyr Val45 50 55atg gcg acg ctt cct tcg aat aca gaa aaa gag gtg ccg acg atc ggt 725Met Ala Thr Leu Pro Ser Asn Thr Glu Lys Glu Val Pro Thr Ile Gly60 65 70 75ttc ttg gct cat gtg gat aca gca aca gat ttt acc gga aag aac gtc 773Phe Leu Ala His Val Asp Thr Ala Thr Asp Phe Thr Gly Lys Asn Val80 85 90aat ccg cag gtt atc gaa caa tac gac gga aag gat att gtg ctg aat 821Asn Pro Gln Val Ile Glu Gln Tyr Asp Gly Lys Asp Ile Val Leu Asn95 100 105gaa tcc ctc aat gtc gtc tta tcg ccc aag gaa ttc ccc gag cta gca 869Glu Ser Leu Asn Val Val Leu Ser Pro Lys Glu Phe Pro Glu Leu Ala110 115 120gat tat gcg gga cat acg ttg att aca acc gac gga acg act ttg ctc 917Asp Tyr Ala Gly His Thr Leu Ile Thr Thr Asp Gly Thr Thr Leu Leu125 130 135ggc gcc gac aat aaa gct ggg atc tct gaa atc atg acg gca atg gaa 965Gly Ala Asp Asn Lys Ala Gly Ile Ser Glu Ile Met Thr Ala Met Glu140 145 150 155tat tta att gcg cac ccc gaa atc aaa cac ggg aag atc aga gtc gct1013Tyr Leu Ile Ala His Pro Glu Ile Lys His Gly Lys Ile Arg Val Ala160 165 170ttt aca cct gat gaa gag atc ggc aga ggg ccg cac aag ttt gac gtc1061Phe Thr Pro Asp Glu Glu Ile Gly Arg Gly Pro His Lys Phe Asp Val175 180 185
gag gcg ttc aat gcc aaa ttc gcg tat acg gtt gac ggc gga ccg ctt1109Glu Ala Phe Asn Ala Lys Phe Ala Tyr Thr Val Asp Gly Gly Pro Leu190 195 200ggc gaa ctg cag tac gaa agc ttc aat gcc gca gcc gcg aaa atc act1157Gly Glu Leu Gln Tyr Glu Ser Phe Asn Ala Ala Ala Ala Lys Ile Thr205 210 215tgt aaa gga acg aac gtc cac ccc ggc aca gca aaa gga aaa atg gtc1205Cys Lys Gly Thr Asn Val His Pro Gly Thr Ala Lys Gly Lys Met Val220 225 230 235aat gcc gcc aaa atc gcg atg cag ttc cac gcc gca ttg ccg gaa aac1253Asn Ala Ala Lys Ile Ala Met Gln Phe His Ala Ala Leu Pro Glu Asn240 2452 50gaa gct cct gaa ttt aca gaa ggt tat gaa ggc ttt tat cat ctg ctt1301Glu Ala Pro Glu Phe Thr Glu Gly Tyr Glu Gly Phe Tyr His Leu Leu255 260 265tca atc aag ggc gat gtt tct gaa acg agc ctc tct tat atc att aga1349Ser Ile Lys Gly Asp Val Ser Glu Thr Ser Leu Ser Tyr Ile Ile Arg270 275 280gat ttt gac aga gac cga ttt aac gag aga aaa gac acc gtc caa aaa1397Asp Phe Asp Arg Asp Arg Phe Asn Glu Arg Lys Asp Thr Val Gln Lys285 290 295atc gca aac aac ctt aaa gcg aaa tac ggc gaa aac agc gtc aca gtg1445Ile Ala Asn Asn Leu Lys Ala Lys Tyr Gly Glu Asn Ser Val Thr Val300 305 310 315gac atg aat gat caa tat tac aac atg agg gaa aag atc gaa ccg gtc1493Asp Met Asn Asp Gln Tyr Tyr Asn Met Arg Glu Lys Ile Glu Pro Val320 325 330aaa gag att gtc gac att gcc tat aaa gcg atg aaa aac ctt gat att1541Lys Glu Ile Val Asp Ile Ala Tyr Lys Ala Met Lys Asn Leu Asp Ile335 340 345gaa ccg gtc gtt aaa ccg atc cgc ggc ggt aca gac ggc tct cag ctc1589Glu Pro Val Val Lys Pro Ile Arg Gly Gly Thr Asp Gly Ser Gln Leu350 355 360tca tat atg ggg ctt cct tgt ccg aac att ttc aca ggc ggg gaa aac1637Ser Tyr Met Gly Leu Pro Cys Pro Asn Ile Phe Thr Gly Gly Glu Asn365 370 375ttt cac gga aaa tac gag tat att tcc gcc gac aat atg gta aaa gcc1685Phe His Gly Lys Tyr Glu Tyr Ile Ser Ala Asp Asn Met Val Lys Ala380 385 390 395gcg aat gtc atc gtg gaa att gtg aag ctg ttt gaa gaa aga gct1730Ala Asn Val Ile Val Glu Ile Val Lys Leu Phe Glu Glu Arg Ala400 405 410taaacgaaaa aagcggaaag cggctgcaag acaaagcagc cgcttttttt tccagatcaa 1790tctttatctg cgctttgctg aaattagaac atgttgcaag gcggtttctt tttggagcgg 1850gataaagcca attaacatct caccgatgca aaaaattctg ttcatttggc ttcatcacct 1910ttccaaatat ctttcaacgt ccagtaagtg aattcttcaa cccgtacgtt gcctttctca 1970gaaaacagct caatccccgc tcttccttcc ttcggatata tgcggcttgt cattgtcgct 2030
tcaccatgat ttgcaaatac ttcaatagac gatctgtcga gaaacagatg caaagtgagc 2090ttctcatccg cttcaagccg tacgtttctc acaccgtccc tcgctttgcc ggactttgaa 2150cagtcgagcg tcagcttttg atcaatcaag ctgtacttga tggttgtttc ttcctgttca 2210attccccgga tctttaagcc 2230<210>70<211>410<212>PRT<213>地衣芽孢桿菌<400>70Met Lys Asn Lys Leu Ile Glu Arg Leu Ile Ser Tyr Ala Lys Val Asp1 5 10 15Thr Gln Ser Asn Glu Asn Ser Gln Thr Thr Pro Ser Thr Pro Gly Gln20 25 30Leu Ala Leu Ala Asn Met Leu Val Glu Glu Leu Lys Glu Ile Gly Met35 40 45Lys Asp Val Thr Ile Asp Glu Asn Gly Tyr Val Met Ala Thr Leu Pro50 55 60Ser Asn Thr Glu Lys Glu Val Pro Thr Ile Gly Phe Leu Ala His Val65 70 75 80Asp Thr Ala Thr Asp Phe Thr Gly Lys Asn Val Asn Pro Gln Val Ile85 90 95Glu Gln Tyr Asp Gly Lys Asp Ile Val Leu Asn Glu Ser Leu Asn Val100 105 110Val Leu Ser Pro Lys Glu Phe Pro Glu Leu Ala Asp Tyr Ala Gly His115 120 125Thr Leu Ile Thr Thr Asp Gly Thr Thr Leu Leu Gly Ala Asp Asn Lys130 135 140Ala Gly Ile Ser Glu Ile Met Thr Ala Met Glu Tyr Leu Ile Ala His145 150 155 160Pro Glu Ile Lys His Gly Lys Ile Arg Val Ala Phe Thr Pro Asp Glu165 170 175Glu Ile Gly Arg Gly Pro His Lys Phe Asp Val Glu Ala Phe Asn Ala180 185 190Lys Phe Ala Tyr Thr Val Asp Gly Gly Pro Leu Gly Glu Leu Gln Tyr195 200 205
Glu Ser Phe Asn Ala Ala Ala Ala Lys Ile Thr Cys Lys Gly Thr Asn210 215 220Val His Pro Gly Thr Ala Lys Gly Lys Met Val Asn Ala Ala Lys Ile225 230 235 240Ala Met Gln Phe His Ala Ala Leu Pro Glu Asn Glu Ala Pro Glu Phe245 250 255Thr Glu Gly Tyr Glu Gly Phe Tyr His Leu Leu Ser Ile Lys Gly Asp260 265 270Val Ser Glu Thr Ser Leu Ser Tyr Ile Ile Arg Asp Phe Asp Arg Asp275 280 285Arg Phe Asn Glu Arg Lys Asp Thr Val Gln Lys Ile Ala Asn Asn Leu290 295 300Lys Ala Lys Tyr Gly Glu Asn Ser Val Thr Val Asp Met Asn Asp Gln305 310 315 320Tyr Tyr Asn Met Arg Glu Lys Ile Glu Pro Val Lys Glu Ile Val Asp325 330 335Ile Ala Tyr Lys Ala Met Lys Asn Leu Asp Ile Glu Pro Val Val Lys340 345 350Pro Ile Arg Gly Gly Thr Asp Gly Ser Gln Leu Ser Tyr Met Gly Leu355 360 365Pro Cys Pro Asn Ile Phe Thr Gly Gly Glu Asn Phe His Gly Lys Tyr370 375 380Glu Tyr Ile Ser Ala Asp Asn Met Val Lys Ala Ala Asn Val Ile Val385 390 395 400Glu Ile Val Lys Leu Phe Glu Glu Arg Ala405 410<210>71<211>2002<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1502)<400>71ttttattatg gggcattttt atcacagttg gtaaatagcg gatttgcccc agctattttt60gaaaatagcg aaaaaagacg tatttataaa ttgtcaacta attatggtga ctataaagtt 120tatacgaagt atgtatcgaa gcaaagtaat aaaggtcaaa acagaatgtg gcattttcca 180
ttcaatttgg atgaagtaaa caacattcaa caagaccaat ctatcaatat gtttgctttt 240atttgcgggg ttgaagattt agttaatagt gagatagtga ttttaagtag agaggaattt 300aatcagtgta tcggtgtagg ttttaaaaca gaaaatcgac gtgtgtcggt taaatctgaa 360aaaggtgcat ggaaccattt agtttatgga actggtatag aactaaaaga aactccactt 420aaggttatta aaaacttgaa taagagatta agtgaatttt caatggtctc tacttgatga 480taattaaaag aggtgattag gtg aat aat gaa tct aat ttt cta atg tac caa 533Val Asn Asn Glu Ser Asn Phe Leu Met Tyr Gln1 5 10acc gaa aat ggc gat acg aaa att caa gtc cgt tta gaa ggt gaa acc 581Thr Glu Asn Gly Asp Thr Lys Ile Gln Val Arg Leu Glu Gly Glu Thr15 20 25gtc tgg atg acg caa aag gca atg gca gag ctg ttt caa aag ggt gtt 629Val Trp Met Thr Gln Lys Ala Met Ala Glu Leu Phe Gln Lys Gly Val30 35 40cca aca ata aat gag cat att aaa aat ata tat gct gaa ggt gag tta 677Pro Thr Ile Asn Glu His Ile Lys Asn Ile Tyr Ala Glu Gly Glu Leu45 50 55aca gag gag gca act att cgg aaa aac cga ata gtt caa gtt gag ggt 725Thr Glu Glu Ala Thr Ile Arg Lys Asn Arg Ile Val Gln Val Glu Gly60 65 70 75tct cgt gaa gtt gaa cgt gaa gtg acc ttt tac aac ctt gaa gtt atc 773Ser Arg Glu Val Glu Arg Glu Val Thr Phe Tyr Asn Leu Glu Val Ile80 85 90att gca gtc ggc tat cgt gtt cga tct cat cgt ggt aca caa ttc cgt 821Ile Ala Val Gly Tyr Arg Val Arg Ser His Arg Gly Thr Gln Phe Arg95 100 105caa tgg gca aca gag cgt tta aat gaa tac atg gta aaa ggt ttt acg 869Gln Trp Ala Thr Glu Arg Leu Asn Glu Tyr Met Val Lys Gly Phe Thr110 115 120atg gat gat gaa cgc tta aaa gaa atg cgg aat att ggg gca gat tat 917Met Asp Asp Glu Arg Leu Lys Glu Met Arg Asn Ile Gly Ala Asp Tyr125 130 135ttt gat gaa tta ttg gaa cgt att cgt gat att cgt gct tcc gaa aga 965Phe Asp Glu Leu Leu Glu Arg Ile Arg Asp Ile Arg Ala Ser Glu Arg140 145 150 155cgt ttt tat tac aaa ata aca gat atc tat gct aca tct att gat tac1013Arg Phe Tyr Tyr Lys Ile Thr Asp Ile Tyr Ala Thr Ser Ile Asp Tyr160 165 170gat ccg aat aca cca att gca aga gaa ttc ttt gca acc gtt caa aat1061Asp Pro Asn Thr Pro Ile Ala Arg Glu Phe Phe Ala Thr Val Gln Asn175 180 185aaa ctc cat ttt gca atc cat gga cat act gct tcg gaa ctg ata atg1109Lys Leu His Phe Ala Ile His Gly His Thr Ala Ser Glu Leu Ile Met190 195 200aaa cga gcg gat gca aca aag cca aac atg ggc tta acg agt tgg aaa1157Lys Arg Ala Asp Ala Thr Lys Pro Asn Met Gly Leu Thr Ser Trp Lys205 210 215ggt gac aaa gtg cgt aaa cat gat gtc act gtg gcg aaa aat tat tta1205Gly Asp Lys Val Arg Lys His Asp Val Thr Val Ala Lys Asn Tyr Leu220 225 230 235
aca caa gaa gaa ctc agc gat tta aac cgt atc gtg aca atg tat tta1253Thr Gln Glu Glu Leu Ser Asp Leu Asn Arg Ile Val Thr Met Tyr Leu240 245 250gac tat gca gaa aca caa gcg aaa aag aaa aag ccg atg tac atg aag1301Asp Tyr Ala Glu Thr Gln Ala Lys Lys Lys Lys Pro Met Tyr Met Lys255 260 265gat tgg gca gaa aaa tta gat gca ttt tta gag ttt aat gaa cat gaa1349Asp Trp Ala Glu Lys Leu Asp Ala Phe Leu Glu Phe Asn Glu His Glu270 275 280ata tta act aac gct ggg aaa att aaa gcc aaa gta gcg gaa caa ttt1397Ile Leu Thr Asn Ala Gly Lys Ile Lys Ala Lys Val Ala Glu Gln Phe285 290 295gca aac gaa caa tat gaa gtg ttt cat caa caa cga tta gca gaa ccg1445Ala Asn Glu Gln Tyr Glu Val Phe His Gln Gln Arg Leu Ala Glu Pro300 305 310 315aag aaa gat gat ttt gat caa ttt ttg gaa caa agg aag cag tta gac1493Lys Lys Asp Asp Phe Asp Gln Phe Leu Glu Gln Arg Lys Gln Leu Asp320 325 330agt gaa aaa tagaaaggat tttaggtgaa tttcatggaa gcatttaagc1542Ser Glu Lysaatacaaatg ggaaaatgga aatcttagtg acattgctga cattactatg ggccagtcgc 1602caccaggtaa ttcctataat gatatcaagg atggaatcgg tttaatcaat ggaccaacag 1662aatttacgaa taaataccca gttgtaaaac aatggacatc taaacctaca aaactttgta 1722aagctgggga tatattattg tgtgtaagag gaagttcaac aggacggatg aatatagctg 1782atgatgaata ttgcattggt cgaggagtag cttctattag agctaaaaaa gataaagctg 1842aaacaagttt tatttattac acattaaatt ataaagttaa tcagttatta caaaagactg 1902cgggttctac tttcccaaat cttagtagta atgaaataaa agatatgatt gtcggtattc 1962cattatttgc agaacaacaa aaaatcgcct ccatcctctc2002<210>72<211>334<212>PRT<213>地衣芽孢桿菌<400>72Val Asn Asn Glu Ser Asn Phe Leu Met Tyr Gln Thr Glu Asn Gly Asp1 5 10 15Thr Lys Ile Gln Val Arg Leu Glu Gly Glu Thr Val Trp Met Thr Gln20 25 30Lys Ala Met Ala Glu Leu Phe Gln Lys Gly Val Pro Thr Ile Asn Glu35 40 45His Ile Lys Asn Ile Tyr Ala Glu Gly Glu Leu Thr Glu Glu Ala Thr50 55 60
Ile Arg Lys Asn Arg Ile Val Gln Val Glu Gly Ser Arg Glu Val Glu65 70 75 80Arg Glu Val Thr Phe Tyr Asn Leu Glu Val Ile Ile Ala Val Gly Tyr85 90 95Arg Val Arg Ser His Arg Gly Thr Gln Phe Arg Gln Trp Ala Thr Glu100 105 110Arg Leu Asn Glu Tyr Met Val Lys Gly Phe Thr Met Asp Asp Glu Arg115 120 125Leu Lys Glu Met Arg Asn Ile Gly Ala Asp Tyr Phe Asp Glu Leu Leu130 135 140Glu Arg Ile Arg Asp Ile Arg Ala Ser Glu Arg Arg Phe Tyr Tyr Lys145 150 155 160Ile Thr Asp Ile Tyr Ala Thr Ser Ile Asp Tyr Asp Pro Asn Thr Pro165 170 175Ile A1a Arg Glu Phe Phe Ala Thr Val Gln Asn Lys Leu His Phe Ala180 185 190Ile His Gly His Thr Ala Ser Glu Leu Ile Met Lys Arg Ala Asp Ala195 200 205Thr Lys Pro Asn Met Gly Leu Thr Ser Trp Lys Gly Asp Lys Val Arg210 215 220Lys His Asp Val Thr Val Ala Lys Asn Tyr Leu Thr Gln Glu Glu Leu225 230 235 240Ser Asp Leu Asn Arg Ile Val Thr Met Tyr Leu Asp Tyr Ala Glu Thr245 250 255Gln Ala Lys Lys Lys Lys Pro Met Tyr Met Lys Asp Trp Ala Glu Lys260 265 270Leu Asp Ala Phe Leu Glu Phe Asn Glu His Glu Ile Leu Thr Asn Ala275 280 285Gly Lys Ile Lys Ala Lys Val Ala Glu Gln Phe Ala Asn Glu Gln Tyr290 295 300Glu Val Phe His Gln Gln Arg Leu Ala Glu Pro Lys Lys Asp Asp Phe305 310 315 320Asp Gln Phe Leu Glu Gln Arg Lys Gln Leu Asp Ser Glu Lys325 330<210>73
<211>2170<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1670)<400>73cgaatacacc aattgcaaga gaattctttg caaccgttca aaataaactc cattttgcaa 60tccatggaca tactgcttcg gaactgataa tgaaacgagc ggatgcaaca aagccaaaca 120tgggcttaac gagttggaaa ggtgacaaag tgcgtaaaca tgatgtcact gtggcgaaaa 180attatttaac acaagaagaa ctcagcgatt taaaccgtat cgtgacaatg tatttagact 240atgcagaaac acaagcgaaa aagaaaaagc cgatgtacat gaaggattgg gcagaaaaat 300tagatgcatt tttagagttt aatgaacatg aaatattaac taacgctggg aaaattaaag 360ccaaagtagc ggaacaattt gcaaacgaac aatatgaagt gtttcatcaa caacgattag 420cagaaccgaa gaaagatgat tttgatcaat ttttggaaca aaggaagcag ttagacagtg 480aaaaatagaa aggattttag gtg aat ttc atg gaa gca ttt aag caa tac aaa 533Val Asn Phe Met Glu Ala Phe Lys Gln Tyr Lys1 5 10tgg gaa aat gga aat ctt agt gac att gct gac att act atg ggc cag581Trp Glu Asn Gly Asn Leu Ser Asp Ile Ala Asp Ile Thr Met Gly Gln15 20 25tcg cca cca ggt aat tcc tat aat gat atc aag gat gga atc ggt tta629Ser Pro Pro Gly Asn Ser Tyr Asn Asp Ile Lys Asp Gly Ile Gly Leu30 35 40atc aat gga cca aca gaa ttt acg aat aaa tac cca gtt gta aaa caa677Ile Asn Gly Pro Thr Glu Phe Thr Asn Lys Tyr Pro Val Val Lys Gln45 50 55tgg aca tct aaa cct aca aaa ctt tgt aaa gct ggg gat ata tta ttg725Trp Thr Ser Lys Pro Thr Lys Leu Cys Lys Ala Gly Asp Ile Leu Leu60 65 70 75tgt gta aga gga agt tca aca gga cgg atg aat ata gct gat gat gaa773Cys Val Arg Gly Ser Ser Thr Gly Arg Met Asn Ile Ala Asp Asp Glu80 85 90tat tgc att ggt cga gga gta gct tct att aga gct aaa aaa gat aaa821Tyr Cys Ile Gly Arg Gly Val Ala Ser Ile Arg Ala Lys Lys Asp Lys95 100 105gct gaa aca agt ttt att tat tac aca tta aat tat aaa gtt aat cag869Ala Glu Thr Ser Phe Ile Tyr Tyr Thr Leu Asn Tyr Lys Val Asn Gln110 115 120tta tta caa aag act gcg ggt tct act ttc cca aat ctt agt agt aat917Leu Leu Gln Lys Thr Ala Gly Ser Thr Phe Pro Asn Leu Ser Ser Asn125 130 135gaa ata aaa gat atg att gtc ggt att cca tta ttt gca gaa caa caa965Glu Ile Lys Asp Met Ile Val Gly Ile Pro Leu Phe Ala Glu Gln Gln140 145 150 155aaa atc gcc tcc atc ctc tct acc tgg gat aag gca att gag cta aaa 1013Lys Ile Ala Ser Ile Leu Ser Thr Trp Asp Lys Ala Ile Glu Leu Lys160 165 170
gaa aag cta atc gag cag aag aaa gag cag aaa aaa ggg tta atg cag1061Glu Lys Leu Ile Glu Gln Lys Lys Glu Gln Lys Lys Gly Leu Met Gln175 180 185aag ttg ctg act ggt aaa gtg aga ttg cct ggg ttt agt gac aaa tgg1109Lys Leu Leu Thr Gly Lys Val Arg Leu Pro Gly Phe Ser Asp Lys Trp190 195 200gaa aag aaa aaa atc ggt gaa cta tta gaa gaa agc aag gta att gca1157Glu Lys Lys Lys Ile Gly Glu Leu Leu Glu Glu Ser Lys Val Ile Ala205 210 215aaa aat ccg caa tta gat aaa agg att act gtg cga tta aat tta aaa1205Lys Asn Pro Gln Leu Asp Lys Arg Ile Thr Val Arg Leu Asn Leu Lys220 225 230 235ggt gtt tgt aaa aga gaa ata tct acg gtg gaa aaa gag gga gca act1253Gly Val Cys Lys Arg Glu Ile Ser Thr Val Glu Lys Glu Gly Ala Thr240 245 250aca caa tat atc aga aaa gaa ggc caa ttt att tat ggg aaa cag aat1301Thr Gln Tyr Ile Arg Lys Glu Gly Gln Phe Ile Tyr Gly Lys Gln Asn255 260 265ctt cat aag ggg gca ttt gga ttg att cca aaa gag tta gat gga ttc1349Leu His Lys Gly Ala Phe Gly Leu Ile Pro Lys Glu Leu Asp Gly Phe270 275 280cag tct tca tct gac ata cct tgt ttt gat ttt aaa gaa ggt gta gat1397Gln Ser Ser Ser Asp Ile Pro Cys Phe Asp Phe Lys Glu Gly Val Asp285 290 295ggt ctc tgg ttt tat tat tat ttt tca aga gaa agc ttc tat act aat1445Gly Leu Trp Phe Tyr Tyr Tyr Phe Ser Arg Glu Ser Phe Tyr Thr Asn300 305 310 315tta gaa aat atc tca agt ggt aca ggt tct aaa cgg atc caa cct aag1493Leu Glu Asn Ile Ser Ser Gly Thr Gly Ser Lys Arg Ile Gln Pro Lys320 325 330gaa tta tac aag tta aca att aaa ctc ccg tca ctt aga gaa caa caa1541Glu Leu Tyr Lys Leu Thr Ile Lys Leu Pro Ser Leu Arg Glu Gln Gln335 340 345aga cag tca aaa att tta gaa tgt agt gat aaa gaa att tat ttg tta1589Arg Gln Ser Lys Ile Leu Glu Cys Ser Asp Lys Glu Ile Tyr Leu Leu350 355 360gag aaa gaa tta gag act tat aga aaa caa aaa caa ggt ctt atg caa1637Glu Lys Glu Leu Glu Thr Tyr Arg Lys Gln Lys Gln Gly Leu Met Gln365 370 375ctt ctt cta aca ggg aaa gtc cga gtg aag gta taattccttt actccttttc 1690Leu Leu Leu Thr Gly Lys Val Arg Val Lys Val380 385 390ctttattctc aacaatggag gtggagaaat gtcgattccg caaagttatg atgaacgtta 1750cattagtcaa cagcctgcga tcgaggtgtt gcaaggctta ggatatgagt acatagaggc 1810tgaacgagct gaggcgatgc gtgagaatct ctatagcgtt ttgctgaaaa cggtattgga 1870aaataaattg aaggagttaa attcctatca gtataaaggt gttacgtaca agttcagtga 1930agcgaacatc caacaagcga tgcgagattt ggatgagcca ctcacaaacg gacttgtaaa 1990ggcaaatgaa agcatttatg aaacgttgat gttaggaaga acttatacgg aattacttcc 2050
agacggctcg aaaaagtcat ttaccattca atatattgat tgggagaatg ttgaaaataa 2110tgtttttcac attgtagagg aattttctgt tgaacgtgta gacggcaggg gtacagttcg 2170<210>74<211>390<212>PRT<213>地衣芽孢桿菌<400>74Val Asn Phe Met Glu Ala Phe Lys Gln Tyr Lys Trp Glu Asn Gly Asn1 5 10 15Leu Ser Asp Ile Ala Asp Ile Thr Met Gly Gln Ser Pro Pro Gly Asn20 25 30Ser Tyr Asn Asp Ile Lys Asp Gly Ile Gly Leu Ile Asn Gly Pro Thr35 40 45Glu Phe Thr Asn Lys Tyr Pro Val Val Lys Gln Trp Thr Ser Lys Pro50 55 60Thr Lys Leu Cys Lys Ala Gly Asp Ile Leu Leu Cys Val Arg Gly Ser65 70 75 80Ser Thr Gly Arg Met Asn Ile Ala Asp Asp Glu Tyr Cys Ile Gly Arg85 90 95Gly Val Ala Ser Ile Arg Ala Lys Lys Asp Lys Ala Glu Thr Ser Phe100 105 110Ile Tyr Tyr Thr Leu Asn Tyr Lys Val Asn Gln Leu Leu Gln Lys Thr115 120 125Ala Gly Ser Thr Phe Pro Asn Leu Ser Ser Asn Glu Ile Lys Asp Met130 135 140Ile Val Gly Ile Pro Leu Phe Ala Glu Gln Gln Lys Ile Ala Ser Ile145 150 155 160Leu Ser Thr Trp Asp Lys Ala Ile Glu Leu Lys Glu Lys Leu Ile Glu165 170 175Gln Lys Lys Glu Gln Lys Lys Gly Leu Met Gln Lys Leu Leu Thr Gly180 185 190Lys Val Arg Leu Pro Gly Phe Ser Asp Lys Trp Glu Lys Lys Lys Ile195 200 205Gly Glu Leu Leu Glu Glu Ser Lys Val Ile Ala Lys Asn Pro Gln Leu210 215 220Asp Lys Arg Ile Thr Val Arg Leu Asn Leu Lys Gly Val Cys Lys Arg
225 230 235 240Glu Ile Ser Thr Val Glu Lys Glu Gly Ala Thr Thr Gln Tyr Ile Arg245 250 255Lys Glu Gly Gln Phe Ile Tyr Gly Lys Gln Asn Leu His Lys Gly Ala260 265 270Phe Gly Leu Ile Pro Lys Glu Leu Asp Gly Phe Gln Ser Ser Ser Asp275 280 285Ile Pro Cys Phe Asp Phe Lys Glu Gly Val Asp Gly Leu Trp Phe Tyr290 295 300Tyr Tyr Phe Ser Arg Glu Ser Phe Tyr Thr Asn Leu Glu Asn Ile Ser305 310 315 320Ser Gly Thr Gly Ser Lys Arg Ile Gln Pro Lys Glu Leu Tyr Lys Leu325 330 335Thr Ile Lys Leu Pro Ser Leu Arg Glu Gln Gln Arg Gln Ser Lys Ile340 345 350Leu Glu Cys Ser Asp Lys Glu Ile Tyr Leu Leu Glu Lys Glu Leu Glu355 360 365Thr Tyr Arg Lys Gln Lys Gln Gly Leu Met Gln Leu Leu Leu Thr Gly370 375 380Lys Val Arg Val Lys Val385 390<210>75<211>1528<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1118)<220>
<221>misc_feature<222>(1518)..(1518)<223>n is a，c，g，or t<400>75cggcggtgtt gcagcattgc tgaccgcgcc gacttcagga aaaaacttga gaggacagct60taaagccaac tgcggtaaat gggaagacac cgtcaaaagg ctgaaaaacg acggaatcgc 120attaaaagag caattcgtca aaaccgccat agaaggcgcg gaaatcatta aagacgtcgg 180cagcgaactg caaacgtcca tccaggaatg gcgcgaggaa atcaaacctc atcagcagga 240cttaaaaaag gaaatcgctg agatcgaaga aaagataaaa cagctggaga aaaccttaca 300
aaactaatat actcagcgaa aaaaataaac cttaaaaaag gggtgtgaat ggaaaatttt 360cacgctcctt ttttaggtaa aatcctgatt tcacaccgta aaactggatt ttttaaaaaa 420ttcgtaaatt tacatcttta ttaatcttta ttttattggc ataatagaaa ttataagata 480aagagaagca ggtgaagtga atg aat cga gcg gaa gag ccc tat acg gtg aaa 533Met Asn Arg Ala Glu Glu Pro Tyr Thr Val Lys1 5 10gaa gcc ctt tta ttc agt caa aga atg gca cag ctt agc aag gcg ctt 581Glu Ala Leu Leu Phe Ser Gln Arg Met Ala Gln Leu Ser Lys Ala Leu15 20 25tgg aag tcg ata gaa aag gat tgg cag cag tgg att aaa cct tat gat 629Trp Lys Ser Ile Glu Lys Asp Trp Gln Gln Trp Ile Lys Pro Tyr Asp30 35 40ctg aac att aac gag cac cat att ttg tgg atc gcc tac cag ctg aac 677Leu Asn Ile Asn Glu His His Ile Leu Trp Ile Ala Tyr Gln Leu Asn45 50 55gga gca tcc att tca gaa atc gcc aag ttc ggc gtc atg cac gta tcc 725Gly Ala Ser Ile Ser Glu Ile Ala Lys Phe Gly Val Met His Val Ser60 65 70 75aca gcg ttt aat ttt tca aaa aag ctt gag gaa aga ggt tac ctg gag 773Thr Ala Phe Asn Phe Ser Lys Lys Leu Glu Glu Arg Gly Tyr Leu Glu80 85 90ttt tcc aaa aaa ttg aat gac aaa cgc aac act tac att cag ctg act 821Phe Ser Lys Lys Leu Asn Asp Lys Arg Asn Thr Tyr Ile Gln Leu Thr95 100 105ccg aaa ggt gaa gaa gtt ttc ctt aaa atc ctc gag tct tac gat ccg 869Pro Lys Gly Glu Glu Val Phe Leu Lys Ile Leu Glu Ser Tyr Asp Pro110 115 120acc cgg aac gcc gtc tta aaa ggc gct cag ccc ctt cat cag ctg tac 917Thr Arg Asn Ala Val Leu Lys Gly Ala Gln Pro Leu His Gln Leu Tyr125 130 135ggg aag ttt cca gaa atc gtc gag atg atg tcg atc atc cgc cat att 965Gly Lys Phe Pro Glu Ile Val Glu Met Met Ser Ile Ile Arg His Ile140 145 150 155tac ggc gat gat ttc atg gaa att ttc gaa aaa tcg ttt tca aac atc1013Tyr Gly Asp Asp Phe Met Glu Ile Phe Glu Lys Ser Phe Ser Asn Ile160 165 170gaa aat gaa ttt acg agc gaa gag ggc aaa atg aag aaa aaa caa gaa1061Glu Asn Glu Phe Thr Ser Glu Glu Gly Lys Met Lys Lys Lys Gln Glu175 180 185gca aaa gaa gcc ggg gaa tcg ata gag gtg gac aaa ccg ctt gag cct1109Ala Lys Glu Ala Gly Glu Ser Ile Glu Val Asp Lys Pro Leu Glu Pro190 195 200ctt aaa aac taagccagat cgtactgcct gataatgctg atgaattcgc1158Leu Lys Asn205tcatcagcgg tttgtaaaga ccttgtctgt gaagggcgaa aatcgtttcg tgcacttcaa 1218gtttttcatt taactgtccg gcaaaaagcg tgaggagatg atcgaacatc acaaatcctt 1278gcgctttttg tgcttcaagc tcctttgaga gctcttcgca cagcttttcc gtttcctttg 1338cttcttcctc tgataagccc ttttcgatca atattttata gtacggatac tcttccatat 1398
ccgcattgtt caataacagt ttgatgtaat attccagccg ctcgaggcgt tgctccattg 1458agttcatatt tgtctcttct ctccctttct cttattttag ccgatattcc cttctgatan 1518aagttcaaca 1528<210>76<211>206<212>PRT<213>地衣芽孢桿菌<400>76Met Asn Arg Ala Glu Glu Pro Tyr Thr Val Lys Glu Ala Leu Leu Phe1 5 10 15Ser Gln Arg Met Ala Gln Leu Ser Lys Ala Leu Trp Lys Ser Ile Glu20 25 30Lys Asp Trp Gln Gln Trp Ile Lys Pro Tyr Asp Leu Asn Ile Asn Glu35 40 45His His Ile Leu Trp Ile Ala Tyr Gln Leu Asn Gly Ala Ser Ile Ser50 55 60Glu Ile Ala Lys Phe Gly Val Met His Val Ser Thr Ala Phe Asn Phe65 70 75 80Ser Lys Lys Leu Glu Glu Arg Gly Tyr Leu Glu Phe Ser Lys Lys Leu85 90 95Asn Asp Lys Arg Asn Thr Tyr Ile Gln Leu Thr Pro Lys Gly Glu Glu100 105 110Val Phe Leu Lys Ile Leu Glu Ser Tyr Asp Pro Thr Arg Asn Ala Val115 120 125Leu Lys Gly Ala Gln Pro Leu His Gln Leu Tyr Gly Lys Phe Pro Glu130 135 140Ile Val Glu Met Met Ser Ile Ile Arg His Ile Tyr Gly Asp Asp Phe145 150 155 160Met Glu Ile Phe Glu Lys Ser Phe Ser Asn Ile Glu Asn Glu Phe Thr165 170 175Ser Glu Glu Gly Lys Met Lys Lys Lys Gln Glu Ala Lys Glu Ala Gly180 185 190Glu Ser Ile Glu Val Asp Lys Pro Leu Glu Pro Leu Lys Asn195 200 205<210>77<211>2104
<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1604)<400>77ggaaacgtca gtccctgtcg gttctgtcca ggatacttta tagacgcctt cagtcagctt 60gacgatatcg gctttttgat cgcgaaccca gcgtccccca accattccgc tgtgaatgcg 120gtaatcgatg gtatggtcat ttttaatgta gatttcatat tcccatccgt tttcatacgt 180atagatcata tggcttccta caaactcttt tacatcttga ttcatatgaa ccgctccttt 240atttgtttat tgtaatcgaa acatgttgat atttacatat ataattataa aatgccgtca 300aaaaagatgt caaacgaaaa tacttcctga gagatttgca caaataaaga agattgttac 360gattaatgtc agaattttga gttatcttag gaaattatgc caatatttag aaaagtgatt 420gtcaaaaaat aagcgattct gtaaaatgaa aaacaaccca taaaaaggaa atgacatggg 480aagaaaggaa ggataaacga ttg aag aca aaa att gcg tat gag gaa gtt gcg 533Leu Lys Thr Lys Ile Ala Tyr Glu Glu Val Ala1 5 10gga atg ctt aat caa tgg tat gtc atg atc aag cgt cac gaa gta tca581Gly Met Leu Asn Gln Trp Tyr Val Met Ile Lys Arg His Glu Val Ser15 20 25caa gcg gtc tcg att aaa tgc gac att gag cac cag ctg ccg aat atg629Gln Ala Val Ser Ile Lys Cys Asp Ile Glu His Gln Leu Pro Asn Met30 35 40gaa gaa aat caa gat ctg ctt ctt tat ttt aat ctt tta gac tat cgg677Glu Glu Asn Gln Asp Leu Leu Leu Tyr Phe Asn Leu Leu Asp Tyr Arg45 50 55cac aag ctg ctg aca gaa gag ttt gcc gct tcc aac aaa ctg ttc gag725His Lys Leu Leu Thr Glu Glu Phe Ala Ala Ser Asn Lys Leu Phe Glu60 65 70 75gat att cag gag caa aaa gcc gat atg caa agc aca gat gac atg att773Asp Ile Gln Glu Gln Lys Ala Asp Met Gln Ser Thr Asp Asp Met Ile80 85 90gaa tat tat tat ttc ttt ttc gct ggc atg tac gaa ttt cat aag aag821Glu Tyr Tyr Tyr Phe Phe Phe Ala Gly Met Tyr Glu Phe His Lys Lys95 100 105gat tat aca aat gca atc aat tat tat aaa tta gcc gag gaa aag ctc869Asp Tyr Thr Asn Ala Ile Asn Tyr Tyr Lys Leu Ala Glu Glu Lys Leu110 115 120agg aca atc ccc gat caa atc gaa atc gcc gaa ttc cat tac aaa ctg917Arg Thr Ile Pro Asp Gln Ile Glu Ile Ala Glu Phe His Tyr Lys Leu125 130 135gct atc gcc tac tat caa atc aaa caa aat ttc ctt tcc tta aac cat965Ala Ile Ala Tyr Tyr Gln Ile Lys Gln Asn Phe Leu Ser Leu Asn His140 145 150 155gcg aaa aca gct cta aaa acc ttc aaa gca cat gat gat tac att caa 1013Ala Lys Thr Ala Leu Lys Thr Phe Lys Ala His Asp Asp Tyr Ile Gln160 165 170
aaa gcg atc agc aac gat atg ctg atc ggg gca aat aaa ctc gat tta1061Lys Ala Ile Ser Asn Asp Met Leu Ile Gly Ala Asn Lys Leu Asp Leu175 180 185ttt cgt ttt gat gaa gcc gaa cag cat tac aag caa gcc ctt aaa gac1109Phe Arg Phe Asp Glu Ala Glu Gln His Tyr Lys Gln Ala Leu Lys Asp190 195 200gcg gca ctg atc aaa cat cat gtc ctc ctc ggc atg gct cac cac aac1157Ala Ala Leu Ile Lys His His Val Leu Leu Gly Met Ala His His Asn205 210 215tta ggg ttg agc tat gtc aat cgc aac ctc ctc aca ttg gct gaa cat1205Leu Gly Leu Ser Tyr Val Asn Arg Asn Leu Leu Thr Leu Ala Glu His220 225 230 235cat ttc aaa gaa gcg ctg ctt atc aaa gag cat gaa gaa tcg gtt tac1253Hi Phe Lys Glu Ala Leu Leu Ile Lys Glu His Glu Glu Ser Val Tyr240 245 250ggc atc cat tcc atg ttt gaa ctg aca cat gtg ctg tac aaa tca aat1301Gly Ile His Ser Met Phe Glu Leu Thr His Val Leu Tyr Lys Ser Asn255 260 265gtt gtc aaa gaa gca cgc aaa ttg tat gaa aaa gga ttt ttc cgt gcg1349Val Val Lys Glu Ala Arg Lys Leu Tyr Glu Lys Gly Phe Phe Arg Ala270 275 280gaa aaa gca gga gaa agg gaa tat ttg tcg aaa ttt aaa ctt att cat1397Glu Lys Ala Gly Glu Arg Glu Tyr Leu Ser Lys Phe Lys Leu Ile His285 290 295gct ctg tat gat gaa cag gat cca ctt acg gtt gaa cat gct tta gaa1445Ala Leu Tyr Asp Glu Gln Asp Pro Leu Thr Val Glu His Ala Leu Glu300 305 3l0 315tat ctt aaa acg atc aat ctc tgg acg gat gta gcg gaa tta aca ttt1493Tyr Leu Lys Thr Ile Asn Leu Trp Thr Asp Val Ala Glu Leu Thr Phe320 325 330gat atc gca ctt tac tat aaa gaa aat gga gat gca gac aaa gct gcc1541Asp Ile Ala Leu Tyr Tyr Lys Glu Asn Gly Asp Ala Asp Lys Ala Ala335 340 345gaa tat ttt gaa gaa tct cat cat gca aga gac caa att ctt aaa aga1589Glu Tyr Phe Glu Glu Ser His His Ala Arg Asp Gln Ile Leu Lys Arg350 355 360acg gag gag tta aag tgaaaaagat gattgccgtt gcgttgactg ccgtctttgc1644Thr Glu Glu Leu Lys365aagccttgtc gttttaagtt tttcgtctca gccgaaggga gatgccgagt ttgccggccg 1704agccattttt cttgacgata agccggctca gctgatggcg ggcagagcga tctttctcga 1764cagctttgac ggaaattcgc cagccgcctg acaaaacacc gcataaccct gcttgtcacc 1824ggacaagctc tcttcgaaaa gcgccgccag cggcgctttt tttatgaaag caaatataac 1884cggcgtccct gcaaatcgct tgcgaagatt aaaaatatga aacacatgac aggaatggcg 1944ctcaccggct tttggtcgaa tcctattgaa aaatctgccc gatttatagt gcttgtcatt 2004ctataaatcc ttataatggt cttaacgcca atgtgaggca tgccaataga cagattgcag 2064gggaaattct atactgaaca gcgcaattta gcatgtaatc2104
<210>78<211>368<212>PRT<213>地衣芽孢桿菌<400>78Leu Lys Thr Lys Ile Ala Tyr Glu Glu Val Ala Gly Met Leu Asn Gln1 5 10 15Trp Tyr Val Met Ile Lys Arg His Glu Val Ser Gln Ala Val Ser Ile20 25 30Lys Cys Asp Ile Glu His Gln Leu Pro Asn Met Glu Glu Asn Gln Asp35 40 45Leu Leu Leu Tyr Phe Asn Leu Leu Asp Tyr Arg His Lys Leu Leu Thr50 55 60Glu Glu Phe Ala Ala Ser Asn Lys Leu Phe Glu Asp Ile Gln Glu Gln65 70 75 80Lys Ala Asp Met Gln Ser Thr Asp Asp Met Ile Glu Tyr Tyr Tyr Phe85 90 95Phe Phe Ala Gly Met Tyr Glu Phe His Lys Lys Asp Tyr Thr Asn Ala100 105 110Ile Asn Tyr Tyr Lys Leu Ala Glu Glu Lys Leu Arg Thr Ile Pro Asp115 120 125Gln Ile Glu Ile Ala Glu Phe His Tyr Lys Leu Ala Ile Ala Tyr Tyr130 135 140Gln Ile Lys Gln Asn Phe Leu Ser Leu Asn His Ala Lys Thr Ala Leu145 150 155 160Lys Thr Phe Lys Ala His Asp Asp Tyr Ile Gln Lys Ala Ile Set Asn165 170 175Asp Met Leu Ile Gly Ala Asn Lys Leu Asp Leu Phe Arg Phe Asp Glu180 185 190Ala Glu Gln His Tyr Lys Gln Ala Leu Lys Asp Ala Ala Leu Ile Lys195 200 205His His Val Leu Leu Gly Met Ala His His Asn Leu Gly Leu Ser Tyr210 215 220Val Asn Arg Asn Leu Leu Thr Leu Ala Glu His His Phe Lys Glu Ala225 230 235 240Leu Leu Ile Lys Glu His Glu Glu Ser Val Tyr Gly Ile His Ser Met245 250 255
Phe Glu Leu Thr His Val Leu Tyr Lys Ser Asn Val Val Lys Glu Ala260 265 270Arg Lys Leu Tyr Glu Lys Gly Phe Phe Arg Ala Glu Lys Ala Gly Glu275 280 285Arg Glu Tyr Leu Ser Lys Phe Lys Leu Ile His Ala Leu Tyr Asp Glu290 295 300Gln Asp Pro Leu Thr Val Glu His Ala Leu Glu Tyr Leu Lys Thr Ile305 310 315 320Asn Leu Trp Thr Asp Val Ala Glu Leu Thr Phe Asp Ile Ala Leu Tyr325 330 335Tyr Lys Glu Asn Gly Asp Ala Asp Lys Ala Ala Glu Tyr Phe Glu Glu340 345 350Ser His His Ala Arg Asp Gln Ile Leu Lys Arg Thr Glu Glu Leu Lys355 360 365<210>79<211>2005<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1505)<400>79acctcgattt gctccgagtt tgtcgcttca atcattttat cggtatcata tatattaaaa 60tccgcataca aatgagcttt cccctttttg gccacgccta cgtacaggca gtcatccctg 120aagttcgtct ttcgaacagc ctgcaaaaat gtcccgtcgc cgccgatgct tgcgataata 180ttggcttcat tgtgatggtg aacgacgcga aatccgtgtt cttcggcaag ctgtttcagt 240tttttgcact gctcgtcggt ttcttcgctt tttttgtaaa agaaataaat gtttcggcgg 300tctgtcacaa tcattctccc ctttgtctgt taagtttaga tttttctgcc atctaacata 360tatgttaaaa tgacctgtga cccttagttt accatgtcca aaaacttctt gtatatgaag 420cggcatcata atgaactttt tctcttcata tacgtactaa gacatgcagc ttttttattc 480aaaggaggag aacagacaaa atg aat gcg aaa aga tgg atc gca ctt gtc atc 533Met Asn Ala Lys Arg Trp Ile Ala Leu Val Ile1 5 10gcg ctg ggc gtt ttt gcg tta tct gcg gtg atg agc ctt ttg ctt gca581Ala Leu Gly Val Phe Ala Leu Ser Ala Val Met Ser Leu Leu Leu Ala15 20 25gta ttt gac acg atg ggg aat gac gga aag atg cag ttt ggc ctc aat629Val Phe Asp Thr Met Gly Asn Asp Gly Lys Met Gln Phe Gly Leu Asn30 35 40
gac acc cag gaa gaa acg gtg ctt gaa caa gga aac agc tca aga aaa 677Asp Thr Gln Glu Glu Thr Val Leu Glu Gln Gly Asn Ser Ser Arg Lys45 50 55atc gcc gtt ctt gaa gtg aac gga acg att tcc gac aat ggc ggc gct 725Ile Ala Val Leu Glu Val Asn Gly Thr Ile Ser Asp Asn Gly Gly Ala60 65 70 75tca ggc ctg ttc agc tct gaa ggc tac aac cac aga tcg ttt ttg caa 773Ser Gly Leu Phe Ser Ser Glu Gly Tyr Asn His Arg Ser Phe Leu Gln80 85 90atg ctt gaa aaa gca aaa gac gat tct gcc gta aag ggc att gtt ctg 821Met Leu Glu Lys Ala Lys Asp Asp Ser Ala Val Lys Gly Ile Val Leu95 100 105cgc gtc aat tct ccg ggt ggc gga gta tac gag agc gct gag ata cat 869Arg Val Asn Ser Pro Gly Gly Gly Val Tyr Glu Ser Ala Glu Ile His110 115 120aag aag ctt gaa gag atc aaa aaa gat acg aaa aaa ccg att tac gta 917Lys Lys Leu Glu Glu Ile Lys Lys Asp Thr Lys Lys Pro Ile Tyr Val125 130 135tca atg gga tcg atg gct gcg tcc ggc ggc tat tac att tca acg cct 965Ser Met Gly Ser Met Ala Ala Ser Gly Gly Tyr Tyr Ile Ser Thr Pro140 145 150 155gcc gac aaa atc ttt gca gcg cct gac acg ctg acg ggt tca ctc ggg1013Ala Asp Lys Ile Phe Ala Ala Pro Asp Thr Leu Thr Gly Ser Leu Gly160 165 170gtc atc atg gaa agc ctt aac tac agc aag ctt gca gag aag ctt gga1061Val Ile Met Glu Ser Leu Asn Tyr Ser Lys Leu Ala Glu Lys Leu Gly175 180 185tta aaa acg gag acg att aaa agc ggt gag ttt aaa gat atc atg tca1109Leu Lys Thr Glu Thr Ile Lys Ser Gly Glu Phe Lys Asp Ile Met Ser190 195 200ccg acg cgc gat atg acg aaa aaa gaa aga gaa atc atg cag tca atg1157Pro Thr Arg Asp Met Thr Lys Lys Glu Arg Glu Ile Met Gln Ser Met205 210 215gtc gat gac gcg tac gaa ggg ttt gta gat gtt att gcc gaa ggg cgc1205Val Asp Asp Ala Tyr Glu Gly Phe Val Asp Val Ile Ala Glu Gly Arg220 225 230 235ggc atg tcc gaa aat gac gtg aaa aaa att gcc gac gga cgt gtc tat1253Gly Met Ser Glu Asn Asp Val Lys Lys Ile Ala Asp Gly Arg Val Tyr240 245 250gac ggc cgc cag gcg aaa cag aac cat ctc att gat gag ctc ggc tat1301Asp Gly Arg Gln Ala Lys Gln Asn His Leu Ile Asp Glu Leu Gly Tyr255 260 265tat gaa gat gcg gtc aaa gcg atg aaa aag gac cac aaa aac ctt gcc1349Tyr Glu Asp Ala Val Lys Ala Met Lys Lys Asp His Lys Asn Leu Ala270 275 280ggc gca tct gtt gtc agc tat gaa gaa tca gcc ggc ctt gcc tcg ctg1397Gly Ala Ser Val Val Ser Tyr Glu Glu Ser Ala Gly Leu Ala Ser Leu285 290 295ttt tca atg act gca aat aag atg ttt aaa agc gaa gct gat ttc tta1445Phe Ser Met Thr Ala Asn Lys Met Phe Lys Ser Glu Ala Asp Phe Leu300 305 310 315
aac att aaa gaa gcg att tct caa tcc ggt gca ccg aga ctc atg tat1493Asn Ile Lys Glu Ala Ile Ser Gln Ser Gly Ala Pro Arg Leu Met Tyr320 325 330tta tat gcc aaa taggagggga ccgaaaaaat ggatgtgact tatgatggaa1545Leu Tyr Ala Lys335aggatcataa tgaattatcg ccgaatgtcg gcgtttccag ggaagaatcg gccccgcatg 1605tagagcatgc ctatgccggt ttttggatca ggctgtttgc ttttctgctt gacggggttg 1665ttatcggcag catcaacaac ttagcggttt cacccgtttt cagcctgctg aacctcccta 1725aagaatcagg gtttttcacc ttttcgcttt attcttttgt gacggcggca gtattttttg 1785cttactttgt cctgatgacg aagtatttcg gacagacgtt gggcaagatg gtattcgggc 1845tgaaggtcgt gtcgcttgtc ccggaaaaag ggctgacatg ggatgtggtc ctgtttaggg 1905agtttatcgg ccgctatatc aacagtttat atattacgta tctcgtagca gcgttttcgc 1965ctaagaaaca agggattcac gatttctttg cggacccctg2005<210>80<211>335<212>PRT<213>地衣芽孢桿菌<400>80Met Asn Ala Lys Arg Trp Il e Ala Leu Val Ile Ala Leu Gly Val Phe1 510 15Ala Leu Ser Ala Val Met Ser Leu Leu Leu Ala Val Phe Asp Thr Met20 25 30Gly Asn Asp Gly Lys Met Gln Phe Gly Leu Asn Asp Thr Gln Glu Glu35 40 45Thr Val Leu Glu Gln Gly Asn Ser Ser Arg Lys Ile Ala Val Leu Glu50 55 60Val Asn Gly Thr Ile Ser Asp Asn Gly Gly Ala Ser Gly Leu Phe Ser65 70 75 80Ser Glu Gly Tyr Asn His Arg Ser Phe Leu Gln Met Leu Glu Lys Ala85 90 95Lys Asp Asp Ser Ala Val Lys Gly Ile Val Leu Arg Val Asn Ser Pro100 105 110Gly Gly Gly Val Tyr Glu Ser Ala Glu Ile His Lys Lys Leu Glu Glu115 120 125Ile Lys Lys Asp Thr Lys Lys Pro Ile Tyr Val Ser Met Gly Ser Met130 135 140
Ala Ala Ser Gly Gly Tyr Tyr Ile Ser Thr Pro Ala Asp Lys Ile Phe145 150 155 160Ala Ala Pro Asp Thr Leu Thr Gly Ser Leu Gly Val Ile Met Glu Ser165 170 175Leu Asn Tyr Ser Lys Leu Ala Glu Lys Leu Gly Leu Lys Thr Glu Thr180 185 190Ile Lys Ser Gly Glu Phe Lys Asp Ile Met Ser Pro Thr Arg Asp Met195 200 205Thr Lys Lys Glu Arg Glu Ile Met Gln Ser Met Val Asp Asp Ala Tyr210 215 220Glu Gly Phe Val Asp Val Ile Ala Glu Gly Arg Gly Met Ser Glu Asn225 230 235 240Asp Val Lys Lys Ile Ala Asp Gly Arg Val Tyr Asp Gly Arg Gln Ala245 250 255Lys Gln Asn His Leu Ile Asp Glu Leu Gly Tyr Tyr Glu Asp Ala Val260 265 270Lys Ala Met Lys Lys Asp His Lys Asn Leu Ala Gly Ala Ser Val Val275 280 285Ser Tyr Glu Glu Ser Ala Gly Leu Ala Ser Leu Phe Ser Met Thr Ala290 295 300Asn Lys Met Phe Lys Ser Glu Ala Asp Phe Leu Asn Ile Lys Glu Ala305 310 315 320Ile Ser Gln Ser Gly Ala Pro Arg Leu Met Tyr Leu Tyr Ala Lys325 330 335<210>81<211>1326<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(869)<400>81tgcgttgact gccgtctttg caagccttgt cgttttaagt ttttcgtctc agccgaaggg 60agatgccgag tttgccggcc gagccatttt tcttgacgat aagccggctc agctgatggc 120gggcagagcg atctttctcg acagctttga cggaaattcg ccagccgcct gacaaaacac 180cgcataaccc tgcttgtcac cggacaagct ctcttcgaaa agcgccgcca gcggcgcttt 240ttttatgaaa gcaaatataa ccggcgtccc tgcaaatcgc ttgcgaagat taaaaatatg 300
aaacacatga caggaatggc gctcaccggc ttttggtcga atcctattga aaaatctgcc 360cgatttatag tgcttgtcat tctataaatc cttataatgg tcttaacgcc aatgtgaggc 420atgccaatag acagattgca ggggaaattc tatactgaac agcgcaattt agcatgtaat 480catttttagg agggagcagc atg gaa gtc ttt gaa gaa tat tta gcg gga att 533Met Glu Val Phe Glu Glu Tyr Leu Ala Gly Ile15 10gat cat ccg gaa cat cgt gcc cgg atg gaa gaa gta ttg ggt tgg gta 581Asp His Pro Glu His Arg Ala Arg Met Glu Glu Val Leu Gly Trp Val15 20 25gcg gag aca ttt cca aat tta acg ccg aaa atc gct tgg aat cag ccg 629Ala Glu Thr Phe Pro Asn Leu Thr Pro Lys Ile Ala Trp Asn Gln Pro30 35 40atg ttt act gat cac ggc aca ttt att atc ggc ttt agc gtc gcg aaa 677Met Phe Thr Asp His Gly Thr Phe Ile Ile Gly Phe Ser Val Ala Lys45 50 55aat cat ttg gct gtc gcc cct gaa agc gct ggg atc gat cgt ttt tct 725Asn His Leu Ala Val Ala Pro Glu Ser Ala Gly Ile Asp Arg Phe Ser60 65 70 75gag gaa att gtg cag gcc ggc tat gat cat acg aag cag ctt gtc cgt 773Glu Glu Ile Val Gln Ala Gly Tyr Asp His Thr Lys Gln Leu Val Arg80 85 90ttc cgc tgg gat cgt ccg gtg gat ttt tca ttg ctt gag aaa atg atc 821Phe Arg Trp Asp Arg Pro Val Asp Phe Ser Leu Leu Glu Lys Met Ile95 100 105gag ttt aat att gcg gat aag gcg gac tgc tcg act ttt tgg cgg aaa 869Glu Phe Asn Ile Ala Asp Lys Ala Asp Cys Ser Thr Phe Trp Arg Lys110 115 120taaaaaagcg caaaaataag gggcatcctc tcatgaaggc gagagagcgc cccgaaagaa 929aaacgctgtt tatctaaaac tccaaggttt cgcgcatgta cgcggcaaac ctacccgtta 989tgatgcttat acatccaaag caagccggat ttcagcaggc gaggcactct gccgatcaac 1049ggccgttcag caactaaacc gaaaccggct tttttgccga gcgacccgag gacgcctttc 1109agcttgaatt tcggcattgt ttcaggaaga ggttcgcctt tccagcgcag ttcaagcact 1169tggacgattt gttccgcctg ggcttcggcc agctgtgcgc tcggcgcgtg aggcaggctt 1229gcacagtcgc cgacaacata aacgtgttcg tctcccggca ggttatggtg cggcgtcagg 1289acgacgcgcc cttgcggatc tttttccaca tcgagat 1326<210>82<211>123<212>PRT<213>地衣芽孢桿菌<400>82Met Glu Val Phe Glu Glu Tyr Leu Ala Gly Ile Asp His Pro Glu His1 5 10 15Arg Ala Arg Met Glu Glu Val Leu Gly Trp Val Ala Glu Thr Phe Pro20 25 30
Asn Leu Thr Pro Lys Ile Ala Trp Asn Gln Pro Met Phe Thr Asp His35 40 45Gly Thr Phe Ile Ile Gly Phe Ser Val Ala Lys Asn His Leu Ala Val50 55 60Ala Pro Glu Ser Ala Gly Ile Asp Arg Phe Ser Glu Glu Ile Val Gln65 70 75 80Ala Gly Tyr Asp His Thr Lys Gln Leu Val Arg Phe Arg Trp Asp Arg85 90 95Pro Val Asp Phe Ser Leu Leu Glu Lys Met Ile Glu Phe Asn Ile Ala100 105 110Asp Lys Ala Asp Cys Ser Thr Phe Trp Arg Lys115 120<210>83<211>1891<212>DNA<213>地衣芽孢桿菌<220>
<221>CDS<222>(501)..(1391)<400>83gtacaatgag aaggatgacg gcttggtcga tggaatattt tttgccgagt tccggatgcc 60ccatcaaatc ttttatatcg cgtttgatcc agttttggat ggctgtcgat tttaaggatg 120tcagttcgca caaatttcca agttcaacaa tttcatttgt ggaaagtccg tgttccgttt 180tttgtttttt ccgttcgtac cgggcaatga attcggggat gtccaaattg tcttccggct 240catcgggcgc caaatcggcc gatctcaaaa gaatcgctag cggactgaca tccagctgcc 300cttttaaagc gtacatcagt ttggccattt ccaggcgagt cagtttaaaa ggtttcatca 360ttccacctca ttatgtatta aaatgaaata gcaaacaata taaacattat cttacgtctt 420atcacttgca ttttcaattc tatgccgtat aatttttatt atacgagttc atttgatctt 480attataacat ggaggtaatc atg gga aaa aga atc ttt ctc ttt ctt tta tcg 533Met Gly Lys Arg Ile Phe Leu Phe Leu Leu Ser1 5 10aac atc tta gtc att acg acg atc ggt atc gtg ctt tcg att atc ggt581Asn Ile Leu Val Ile Thr Thr Ile Gly Ile Val Leu Ser Ile Ile Gly15 20 25tcg ctg aca ggg gta ggc acg tat ttc acg gct aac ggc ggc atc gat629Ser Leu Thr Gly Val Gly Thr Tyr Phe Thr Ala Asn Gly Gly Ile Asp30 35 40att gtt gcg ctt ctc gta ttt agt gca gtt gtc ggt ttt gtc ggc tcc677Ile Val Ala Leu Leu Val Phe Ser Ala Val Val Gly Phe Val Gly Ser45 50 55ttt atg tcg ctc ttg atg tca aga tgg atg gcg aaa atg gcg atg ggc725
Phe Met Ser Leu Leu Met Ser Arg Trp Met Ala Lys Met Ala Met Gly60 65 70 75gtt cag gtg ctg aat cct gat aag caa acg ctc agc tat gaa gaa cag 773Val Gln Val Leu Asn Pro Asp Lys Gln Thr Leu Ser Tyr Glu Glu Gln80 85 90cag ctc gtt gac cgg gta tat aag ctg tcc cgc gct gcc gga ctg aca 821Gln Leu Val Asp Arg Val Tyr Lys Leu Ser Arg Ala Ala Gly Leu Thr95 100 105aag atg cct gaa gtc ggc atc tac aat tca cgc gaa gtg aat gcc ttt869Lys Met Pro Glu Val Gly Ile Tyr Asn Ser Arg Glu Val Asn Ala Phe110 115 120gcg acc ggg ccg tct aaa aac cgc tcg ctt gtc gct gta tcg acg ggc 917Ala Thr Gly Pro Ser Lys Asn Arg Ser Leu Val Ala Val Ser Thr Gly125 130 135ctt ctt cag gaa atg gat gac gac gca gtg gaa ggc gta ctt gcc cac 965Leu Leu Gln Glu Met Asp Asp Asp Ala Val Glu Gly Val Leu Ala His140 145 150 155gaa gtg gcg cat att gca aac ggc gat atg gtg acg atg acg ctc ctt1013Glu Val Ala His Ile Ala Asn Gly Asp Met Val Thr Met Thr Leu Leu160 165 170caa gga atc gtc aat acg ttc gtt gtt ttc ttc gca agg att gcg gca1061Gln Gly Ile Val Asn Thr Phe Val Val Phe Phe Ala Arg Ile Ala Ala175 180 185tgg gct gtt tca cgc gtt gtc cgt gaa gag ctg gcg ccg atc gtt cac1109Trp Ala Val Ser Arg Val Val Arg Glu Glu Leu Ala Pro Ile Val His190 195 200ttt atc gcg gtc atc gta ttc caa att gtg ttc tct att ctc gga agc1157Phe Ile Ala Val Ile Val Phe Gln Ile Val Phe Ser Ile Leu Gly Ser205 210 215ctt gtc gtg ttt gcg tac tcc cgc cac cgc gaa tat cat gcg gat aga1205Leu Val Val Phe Ala Tyr Ser Arg His Arg Glu Tyr His Ala Asp Arg220 225 230 235gga gga gca gac ctt gca ggc aag gat aag atg att cac gcg ctc cgc1253Gly Gly Ala Asp Leu Ala Gly Lys Asp Lys Met Ile His Ala Leu Arg240 245 250tcg ctt gag cag tac act tca aga gtc aaa gaa gaa cag gca tct gtt1301Ser Leu Glu Gln Tyr Thr Ser Arg Val Lys Glu Glu Gln Ala Ser Val255 260 265gca act tta aaa atc aac ggg aaa aag cat aca tcg ctt ttc tca aca1349Ala Thr Leu Lys Ile Asn Gly Lys Lys His Thr Ser Leu Phe Ser Thr270 275 280cac cct gat tta agc gac aga atc cgc cgt ctg gaa gca aaa1391His Pro Asp Leu Ser Asp Arg Ile Arg Arg Leu Glu Ala Lys285 290 295taataaagat gcaaaccccc tttttcaagg gggtttgctt tttaaattta aaaatcgaaa 1451agcggctgta cataatagca tgtttccttt aaaatggaac acgttataca caagaagcaa 1511cttttgaata ttgaaaagaa ggaacgatta tgaaggtttt attgagaaaa ctactgcgcg 1571tgctgtctcc tgtacagctg atcgcgctct attatttttt agcggtgaca gtttcggttg 1631ttttattgag tctgcccgtt gcacacaaaa ataatgttga atggtctttt atcgacgccc 1691
tttttaccgc ggtgagcgca gtgagtgtaa ccggtttgac ggttgttgat acagccgata 1751cattcagcac ggccggaatt tggattttgg cgtttgttct gcaatttggc gggatcggcg 1811tgatggcttt gggtacattc gtctggctga ttttcggcaa gcggatcggt ttgaaagagc 1871gccgtctgat tatgaccgat 1891<210>84<211>297<212>PRT<213>地衣芽孢桿菌<400>84Met Gly Lys Arg Ile Phe Leu Phe Leu Leu Ser Asn Ile Leu Val Ile1 5 10 15Thr Thr Ile Gly Ile Val Leu Ser Ile Ile Gly Ser Leu Thr Gly Val20 25 30Gly Thr Tyr Phe Thr Ala Asn Gly Gly Ile Asp Ile Val Ala Leu Leu35 40 45Val Phe Ser Ala Val Val Gly Phe Val Gly Ser Phe Met Ser Leu Leu50 55 60Met Ser Arg Trp Met Ala Lys Met Ala Met Gly Val Gln Val Leu Asn65 70 75 80Pro Asp Lys Gln Thr Leu Ser Tyr Glu Glu Gln Gln Leu Val Asp Arg85 90 95Val Tyr Lys Leu Ser Arg Ala Ala Gly Leu Thr Lys Met Pro Glu Val100 105 110Gly Ile Tyr Asn Ser Arg Glu Val Asn Ala Phe Ala Thr Gly Pro Ser115 120 125Lys Asn Arg Ser Leu Val Ala Val Ser Thr Gly Leu Leu Gln Glu Met130 135 140Asp Asp Asp Ala Val Glu Gly Val Leu Ala His Glu Val Ala His Ile145 150 155 160Ala Asn Gly Asp Met Val Thr Met Thr Leu Leu Gln Gly Ile Val Asn165 170 175Thr Phe Val Val Phe Phe Ala Arg Ile Ala Ala Trp Ala Val Ser Arg180 185 190Val Val Arg Glu Glu Leu Ala Pro Ile Val His Phe Ile Ala Val Ile195 200 205Val Phe Gln Ile Val Phe Ser Ile Leu Gly Ser Leu Val Val Phe Ala
210 215 220Tyr Ser Arg His Arg Glu Tyr His Ala Asp Arg Gly Gly Ala Asp Leu225 230 235 240Ala Gly Lys Asp Lys Met Ile His Ala Leu Arg Ser Leu Glu Gln Tyr245 250 255Thr Ser Arg Val Lys Glu Glu Gln Ala Ser Val Ala Thr Leu Lys Ile260 265 270Asn Gly Lys Lys His Thr Ser Leu Phe Ser Thr His Pro Asp Leu Ser275 280 285Asp Arg Ile Arg Arg Leu Glu Ala Lys290 29權(quán)利要求
1.一種地衣芽孢桿菌突變體宿主細(xì)胞，其衍生自親代地衣芽孢桿菌宿主細(xì)胞，其中所述突變體宿主細(xì)胞有一或多個基因發(fā)生突變，所述基因編碼一或多種具有蛋白水解活性并且與SEQ ID NO2到84所示的一或多種多肽至少80％相同的多肽，其中所述突變體宿主細(xì)胞表達(dá)的所述一或多種具有蛋白水解活性的多肽，比在等同條件下培養(yǎng)的親代宿主細(xì)胞少至少5％。
2.根據(jù)權(quán)利要求1所述的宿主細(xì)胞，其通過一或多個基因的部分或完全缺失進(jìn)行突變，所述基因編碼所述一或多種具有蛋白水解活性的多肽。
3.根據(jù)權(quán)利要求1-2任一所述的宿主細(xì)胞，其在兩或多個編碼兩或多種具有蛋白水解活性的多肽的基因中突變。
4.根據(jù)權(quán)利要求1-3任一所述的宿主細(xì)胞，其包含一或多個編碼一或多種異源多肽的異源基因。
5.根據(jù)權(quán)利要求4所述的宿主細(xì)胞，其中所述異源基因以至少兩個拷貝存在。
6.根據(jù)權(quán)利要求4或5所述的宿主細(xì)胞，其中所述異源基因被穩(wěn)定整合到所述細(xì)胞的基因組。
7.根據(jù)權(quán)利要求4-6任一所述的宿主細(xì)胞，其中所述異源基因被整合到所述細(xì)胞的基因組，而沒有在整合位點(diǎn)留下任何抗生素抗性標(biāo)記物基因。
8.根據(jù)權(quán)利要求4-7任一所述的宿主細(xì)胞，其中所述異源基因從異源啟動子或人工啟動子轉(zhuǎn)錄。
9.根據(jù)權(quán)利要求4-8任一所述的宿主細(xì)胞，其中所述異源基因被包含在操縱子，優(yōu)選多順反子操縱子中。
10.根據(jù)權(quán)利要求4-9任一所述的宿主細(xì)胞，其中所述異源多肽是抗微生物肽或融合肽，所述融合肽包含當(dāng)為天然形式時具有抗微生物活性的肽部分。
11.根據(jù)權(quán)利要求4-9任一所述的宿主細(xì)胞，其中所述異源多肽具有生物合成的活性，并產(chǎn)生感興趣的化合物或中間體。
12.根據(jù)權(quán)利要求11所述的宿主細(xì)胞，其中所述感興趣的化合物或中間體包含維生素，氨基酸，抗生素，碳水化合物或表面活性劑。
13.根據(jù)權(quán)利要求12所述的宿主細(xì)胞，其中所述碳水化合物包含透明質(zhì)酸。
14.根據(jù)權(quán)利要求4-9任一所述的宿主細(xì)胞，其中所述異源多肽是酶，優(yōu)選分泌型酶。
15.根據(jù)權(quán)利要求14所述的宿主細(xì)胞，其中所述酶是選自如下酶類的酶氧化還原酶(EC1)，轉(zhuǎn)移酶(EC2)，水解酶(EC3)，裂合酶(EC4)，異構(gòu)酶(EC5)，和連接酶(EC6)。
16.根據(jù)權(quán)利要求15所述的宿主細(xì)胞，其中所述酶具有選自如下酶的活性氨肽酶，淀粉酶，淀粉葡萄糖苷酶，甘露聚糖酶，糖酶，羧肽酶，過氧化氫酶，纖維素酶，幾丁質(zhì)酶，角質(zhì)酶，環(huán)糊精糖基轉(zhuǎn)移酶，脫氧核糖核酸酶，酯酶，半乳糖苷酶，β-半乳糖苷酶，葡糖淀粉酶，葡萄糖氧化酶，葡糖苷酶，鹵過氧化物酶，半纖維素酶，轉(zhuǎn)化酶，異構(gòu)酶，漆酶，連接酶，脂酶，裂合酶，甘露糖苷酶，氧化酶，果膠酶，過氧化物酶，肌醇六磷酸酶，酚氧化酶，多酚氧化酶，蛋白酶，核糖核酸酶，轉(zhuǎn)移酶，轉(zhuǎn)谷氨酰胺酶和木聚糖酶。
17.根據(jù)權(quán)利要求16所述的宿主細(xì)胞，其中所述酶是淀粉酶或甘露聚糖酶。
18.在地衣芽孢桿菌突變體宿主細(xì)胞中產(chǎn)生至少一種感興趣的產(chǎn)物的方法，其包括在合適的培養(yǎng)基中培養(yǎng)權(quán)利要求1-17任一限定的地衣芽孢桿菌突變體宿主細(xì)胞，在該培養(yǎng)基中產(chǎn)生所述產(chǎn)物。
19.根據(jù)權(quán)利要求18所述的方法，其還包括分離或純化所述感興趣的產(chǎn)物。
20.權(quán)利要求1-17任一所限定的地衣芽孢桿菌突變體宿主細(xì)胞在制備至少一種感興趣的產(chǎn)物中的用途，其包含在適合的培養(yǎng)基中培養(yǎng)所述突變體宿主細(xì)胞，在該培養(yǎng)基中產(chǎn)生所述產(chǎn)物。
21.根據(jù)權(quán)利要求20的用途，其還包含分離或純化所述感興趣的產(chǎn)物。
全文摘要
一種地衣芽孢桿菌突變體宿主細(xì)胞，其包含一或多個基因突變(缺失)，所述基因編碼具有蛋白水解活性的多肽，其中所述突變體宿主細(xì)胞表達(dá)的所述一或多種蛋白酶，比在等同條件下培養(yǎng)的親代宿主細(xì)胞少至少5％。所述突變體宿主細(xì)胞用于制備異源多肽。
文檔編號C12N9/32GK1659282SQ03813316
公開日2005年8月24日 申請日期2003年3月25日 優(yōu)先權(quán)日2002年4月10日
發(fā)明者詹斯·T·安德森, 斯蒂恩·T·喬根森, 邁克爾·D·拉斯馬森, 彼得·B·奧爾森, 伊布·G·克勞森 申請人:諾維信公司