專利名稱：日本血吸蟲表皮特異抗原基因、蛋白及其應用的制作方法
技術領域：
本發(fā)明涉及基因工程技術領域，具體地說涉及一類表達于日本血吸蟲表皮的蛋白質、其編碼核苷酸和編碼氨基酸序列以及其作為藥物靶點和疫苗的作用。
背景技術：
目前研究表明，血吸蟲病由血吸蟲的感染引起，在世界上有76個國家，受感染者約1.5億。致病的血吸蟲有3種，既日本血吸蟲(Schistosoma japonicum)，曼氏血吸蟲(S.mansoni)和埃及血吸蟲(S.haematobium)。在我國流行的是日本血吸蟲。
研制新的治療血吸蟲病和抗血吸蟲的藥物，首先必須找到合適的藥物作用靶點。血吸蟲的表皮是宿主和血吸蟲相互作用的界面。而組成血吸蟲表皮的蛋白質暴露在這個界面上，對小分子的化學藥物和大分子的抗體藥物而言都是最理想的藥物作用靶點。已經(jīng)有研究表明治療血吸蟲的化學藥物——比喹酮的作用靶點就是存在于血吸蟲的表皮。另外一些已知的血吸蟲表皮蛋白，例如GST28，paramyosin，已經(jīng)用于疫苗研究和應用。

發(fā)明內(nèi)容
本發(fā)明要解決的技術問題是提供一類表達于日本血吸蟲表皮的蛋白質、其編碼核苷酸、及編碼氨基酸序列日本血吸蟲表皮蛋白，作為可能的藥物作用靶點和候選疫苗方面的作用。
為解決上述技術問題，本發(fā)明提供一種DNA序列，它具有序列表中SEQ IDNO.1～SEQ ID NO.23所述序列中任一序列的堿基序列；或者它是在中度嚴緊條件下與序列表中SEQ ID NO.1～SEQ ID NO.23所示的序列中任一條序列雜交的序列；或者它是與序列表中SEQ ID NO.1～SEQ ID NO.23所示的序列中任一條序列有至少70％同源性的序列。
為解決上述技術問題，本發(fā)明還提供一種氨基酸序列，它具有序列表中SEQID NO.24～SEQ ID NO.46所述序列中任一序列的氨基酸序列；或者它是與序列表中SEQ ID NO.24～SEQ ID NO.46所示的序列中任一條序列有至少70％同源性的序列；或者它是包含有序列表中SEQ ID NO.24～SEQ ID NO.46所示的序列中任一條序列的氨基酸序列。
本發(fā)明由于通過大規(guī)模的蛋白質組學研究，發(fā)現(xiàn)了23個日本血吸蟲表皮蛋白質，這些蛋白質，都是可能的藥物作用靶點和候選疫苗。血吸蟲病的流行給人民身體健康和經(jīng)濟發(fā)展都造成巨大的損失，因此，防治血吸蟲病、開發(fā)研制治療血吸蟲病和抗血吸蟲的藥物具有巨大的社會和經(jīng)濟效益。


圖1是免疫熒光抗體原位雜交實驗結果。
具體實施例方式
下面結合附圖和具體實施例對本發(fā)明作進一步詳細的說明。
實施例1日本血吸蟲表皮蛋白的分離和鑒定實驗1.日本血吸蟲表皮的分離純化血吸蟲表皮的純化按照文獻報道的Triton-x-100提取方法進行(參見Oaks，J.A.等人，寄生蟲學雜志，69(3)519-533(1983)。)。表皮蛋白從血吸蟲雄蟲、雌蟲、雌雄混合成蟲和童蟲分離。蟲體用Krebs’-Ringer’s，Tris-馬來酸鹽-緩沖鹽液(KRTM)洗3次，再用KRTM/0.2％Triton X-100溶液懸浮。這個液體和蟲體的混和物置于冰水浴中，在搖床上搖動10分鐘。然后，裝有液體和蟲體的混和物的試管在渦旋混合器上混旋幾次，然后再放回冰水浴中，靜置使蟲體下沉。用吸管吸出上部液體，放置到另一個新管中。這個試管在離心機上用2500×g的離心力，離心15分鐘。得到的沉淀就是血吸蟲的表皮。Krebs’-Ringer’s溶液的配方是0.15M NaCl，6.2mM KCl，1.2mM Na2HPO4，1.2mM MgSO4，10mM Glucose and 20mM Tris-Hepes，pH7.4。Tris maleate緩沖溶液的配方是0.2M Tris and 0.2MMaleicanhyclride，pH 7.4。
2.日本血吸蟲表皮蛋白的電泳分離日本血吸蟲表皮用裂解劑裂解，蛋白溶液用SDS-聚丙烯酰胺凝膠電泳分離，條帶切割后再做第二維的在線毛細液相色譜分離以及質譜鑒定。軟件分析得到鑒定蛋白。分離好的血吸蟲表皮，用SDS-聚丙烯酰胺凝膠電泳(SDS-PAGE)緩沖液懸浮，用超聲波促進組織細胞裂解和蛋白溶解，超聲波裂解方法為超聲1秒，暫停3秒，總時間2分鐘。并用100℃加熱5分鐘以使蛋白質變性。然后這些表皮蛋白混合物用SDS-PAGE分離，分離方法按照普通的SDS凝膠電泳方法進行。膠上電泳泳道用刀切割下來，再用刀均等分割成8-12份。接下來做蛋白的膠內(nèi)酶解。
3.日本血吸蟲表皮蛋白的膠內(nèi)酶解過程膠內(nèi)酶解參照文獻(Shevchenko，A.等人，化學年報，68850-858(1996)。)的方法進行。蛋白膠塊用刀切碎，每個小塊大小約為1立方毫米。加入100mMNH4HCO3，洗2次；轉入到試管中；加入10mM二硫蘇糖醇(dithiothreitol，DTT)/100mM NH4HCO3，56度放置反應1小時；冷卻到室溫，離心吸去DTT；加入等體積的55mM碘乙酰胺iodoacetamide/100mM NH4HCO3，室溫/黑暗反應45分鐘；吸去iodoacetamide，加入100mM NH4HCO3，洗2次；去掉NH4HCO3，加入乙腈縮膠；去掉乙腈，真空抽干；加入100mM NH4HCO3，膠膨脹，去掉NH4HCO3，加入乙腈縮膠；去掉乙腈，抽干；加入含Trypsin的消化緩沖液(50mM NH4HCO3/12.5ng/ul trypsin)冰上放置45min；去掉多余酶液，加入50mM NH4HCO3，以剛覆蓋膠為準；37度，過夜酶切；酶切結束后，從試管中吸出上清液到新管子中，膠中加入60％ACN/0.5％HAC，在水浴中超聲處理15min，再重復一次；吸出上清液到新管子中，在原管子中再加入60％ACN/0.5％HAC，37度40min，吸出上清液，與前面的上清液合并，真空抽氣去ACN。
4.日本血吸蟲表皮蛋白酶解肽段的在線色譜分離和質譜鑒定酶解產(chǎn)生的肽混合物，用真空將溶液體積抽減到30μl。之后，每個組分的肽混合物上樣到一個反相捕獲柱(C18，5μm，300，300μm i.d×5mm，LC Packings)，在20μl/min的流速下脫鹽。然后這個捕獲柱和一個分析用的75μm×150mm C18柱(LC Packings)相連接，肽混合物就在200nl/min的流速下，從這個柱子上洗脫進入裝有Protana NanoES的電噴霧離子源的QSTAR pulser i中。Agilent 1100毛細液相系統(tǒng)(Agilent Technologies)用來提供流動相A(0.5％乙酸/水)和流動相B(0.5％乙酸/乙腈)，線性梯度是60分鐘內(nèi)5％B到50％B，30分鐘內(nèi)50％B到90％B，然后是在90％B停留15分鐘。在分析柱后通過一個不銹鋼的兩通(Valco Instrument)連接一個10-μm直徑的PicoTip納噴霧頭(New Objective)，加上2500-3000伏的噴霧電壓，以獲得穩(wěn)定的噴霧。MS/MS串聯(lián)質譜譜圖通過信息依賴的采集(IDA)和任務-循環(huán)增強來記錄。在每個測定掃描中，選取3-6個信號最強、帶2到3個電荷的離子，用滾動碰撞能量打碎，以獲得串聯(lián)質譜的信息。
5.日本血吸蟲表皮蛋白的軟件搜索鑒定用牛胰酶的自裂解片段如([M+H]+)1153.5741，2163.0569和2274.1600作為內(nèi)標，或者以預先數(shù)據(jù)庫搜索得到的得分高的肽段的分子量，來校正所有的質譜譜圖。質譜搜索用的數(shù)據(jù)庫，包括從8420個從EST cluster預測得到的血吸蟲蛋白質序列，以及可能的污染蛋白質序列如牛胰酶的序列、宿主蛋白污染如人蛋白質序列、小鼠蛋白質序列、大鼠蛋白質序列、兔子蛋白質序列和角蛋白序列等。搜索軟件是MASCOT(http://www.matrixscience.com/，MatrixScience)。搜索參數(shù)為最大漏切點，3個；monoisotopic；肽電荷數(shù)，+1/+2/+3；修飾，半胱氨酸carbamoylmethylation和methionine氧化。搜索結果合并后，用下列標準進行過濾篩選1.對每一個串聯(lián)質譜譜圖的搜索結果列表，只提取排位在第一個的結果，其他的則丟棄；2.搜索得到的肽段中如果有KR，KK，RK，RR等序列，則這個肽段丟棄；3.搜索得到的肽段，長度在8個氨基酸以上的保留，小于8個氨基酸的丟棄；4.重復打到的肽段，只計算其中分值最高的，把這些肽段分值加起來，做為蛋白的得分；5.蛋白的得分超過60，就認為是可信的結果。低分的蛋白，手工檢查后保留或者丟棄。匹配到宿主或者牛胰酶、角蛋白的肽段，則丟棄。
實施例2日本血吸蟲表皮蛋白的生物信息學分析和功能注釋
1.同源性和相似性分析日本血吸蟲表皮蛋白序列對GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余數(shù)據(jù)庫(Non-redundant，NR)做BLASTP比對；日本血吸蟲表皮蛋白序列對Swissprot數(shù)據(jù)庫做BLASTP同源性比對。根據(jù)比對結果得到的與已知其他物種或已知的血吸蟲蛋白的序列相似性，做出日本血吸蟲表皮蛋白的功能注釋。
2.文獻查檢根據(jù)已經(jīng)發(fā)表的文獻報道，發(fā)現(xiàn)鑒定得到的23個表皮蛋白在蟲體上的位置，有一些的確在血吸蟲表皮上。而其他則是新發(fā)現(xiàn)的表皮蛋白。
舉例說明，比如以下表皮分離和質譜鑒定得到的蛋白，根據(jù)文獻報道，就是已知的表皮蛋白，而且有些蛋白已經(jīng)有進行了疫苗和抗原研究。
paramyosintropomyosinmyosindynein light chain 122.6 kDa tegument membrane-associated antigentegumental antigen Sm20nitric oxide synthase 1(NOS1)leucine aminopeptidase14-3-3 proteinsSnaKcathepsin B-like cysteine protease precursor
21.7 kDa antigenFK506-binding protein 50(immunophilin)實施例3日本血吸蟲表皮蛋白的實驗驗證為了進一步用實驗的方法驗證用蛋白質組學手段鑒定得到的血吸蟲表皮蛋白，選取了一個Immunophilin(SjFKBP50，GenBank登錄號為AY815389)做免疫熒光抗體原位雜交做蛋白定位分析。
實驗方法血吸蟲成蟲用冷凍包埋劑(OCT)完全包埋，切成5μm厚的切片。用抗Immunophilin的抗體覆蓋切片，在37℃反應60分鐘。該抗體是用重組表達的Immunophilin免疫小鼠制備的單克隆抗體。隨后切片用PBS洗滌，再加入FITC-偶聯(lián)的兔抗鼠免疫球蛋白的抗體(1∶40稀釋，用含有0.5mg/ml的Evans藍的PBS稀釋)，置濕盒中37℃反應60分鐘。染色結果用Leica DM-RB型熒光顯微鏡觀察并拍照。
圖一所示為免疫熒光抗體原位雜交實驗結果，表皮T；腸道G；下表皮ST。綠色熒光部分是Immunophilin的信號。
該結果證明了Immunophilin確實分布在血吸蟲成蟲的表皮上。這說明前面表皮分離和質譜鑒定的結果是可信的。
序列表<110>上海人類基因組研究中心<120>日本血吸蟲表皮特異抗原基因、蛋白及其應用<130>NP-10263<160>46<170>PatentIn version 3.1<210>1<211>1908<212>DNA<213>Schistosoma japonicum<400>1atcaaattaa gtatcaaaga attaattaat aatatttaac gatgccgtta acctcacgtt 60cacctcatga cagtaaaaca ctacttgcta aatattacga tttaccgcaa cctgatgata120aagttcaagt tatgtatgta tggatagatg gaagtggaga gaatttgcgc tgtaaaacaa180tgactttacc tgatgaacca caagctccag aagattgtcc catgtggaat ttcgacggaa240gtagtacagg tcaagctgaa ggttccaatt ctgatgttta tctaaaaccg tgtgcaatgt300tccgtgatcc atttcgtggt ggtaaaaata aattagtctt atgtgaaacc tttaattatg360ataaaaaacc acatgtttca aaccatcgtt atctttgtaa tttagtcatg gagaaagcaa420aaattcatga accatggttt ggtattgaac aagaatatgc attactagat attgatggtt480atccattaca atggcctaaa aatggtttcc ctccacctca gggaccttat tattgttctg540tcggggctgg taaagcactt ggtcgcgata taccagaagc tctatacaga gcttgtatgt600atgcaggagt caaaatttgt ggtagcaatg cagaagttat gccatcacag tgggaatttc660aagttggacc atgtgaaggt gtttcagccg gtgatcattt atggatggct agatttatat720tacatcgtgt agctgaagat ttcggtgtta ttgtcacact ggatcctaaa ccgatgcctg780ggaattggaa tggttctgga gctcatacaa actattctac tttggctatg agaaatccaa840aatcaggctt acaagcaata gaggatgctg ttgagaaact gtcgcataaa cacaccgagc900atattaaatg ttatgatcct aaacatggtt tagacaatca aagacgtttg actggtagtc960atgaaacaag cagtattaat gatttttcat cgggtgttgc aaatagaggc agtagtatac 1020gtattccaag acaagttgct gaaaatggtt gcggttattc tggaagatag acgcccgtca 1080tctaattgtg atccatacgt agtcactaga atgttggtag aaacaacatg tttttaacca 1140ctatcattat tatttttttt caaattgaca aaaaaatttc gaaaaaatat caaagaaatt 1200aatatttaca aaattactct tacattacat aatctctgtg tctcatgtaa tcaatttaac 1260ttaaacacgt cattctatta atgattacaa taaattagca gatttgttct aataattctg 1320gtcatcatta tcgttattgt cattgtcatc ttcattacag tttttttcat cgttaacagt 1380catcattgtc ataagaatta ttaacagcaa caacaacatc agaatcagca tcactttcat 1440gttgtccacc gatgattcaa catagttttt tttccatgta tttttagcac ttttttattg 1500atatatattc atagattact taacaaccta attgattgtc ttttgatcac tataagtcat 1560tacatcattt aaactatttc tatacaaatc aaaatattga atgttagatg ttctttttgt 1620tatacttcaa acatcaagtt ttctacttct tgttctatac tactgatttt actcaaaatt 1680
ttcatttgtg attttcattt aagtaatcag gatttttgtt gttacttatt tttaaaattg 1740tgaataaaaa tattagaaac aaaaaaaaaa gaatattctt cagttgagag catttctcct 1800ttgattacat aataaattat agtcttgaat atgattgatt gcgaatgaaa agtgattgct 1860aaacacagag tactgaaaaa ttattattat tattatattc taatttgt1908<210>2<211>1195<212>DNA<213>Schistosoma japonicum<400>2cggggggcac gttttgcagt tcacgttaac atggctgtta caaggcctat tgtcaatgtt 60tatggagtga acgggacgtc aacgggagcc accataaaga tgccggcggt atttactact120ccgcttcgct tggatctact gaatttcgtt catgatcaga tgcgaaaaaa ctcaagacaa180ccatatgcgg ttagtacaaa agctggccat caaacttctg ctgagtcctg gggtactggg240agagcagttg ctcgtatacc tagagtacgt ggtggcggta cacaccggtc aggccaaggt300gcttacggta atatgtgtcg tggtggtcat atgtatgctc caactaagac gatgagacga360tggcacagaa aaataaatat tcagcagcgg cgttatgcca tttgttctgc aatcgctgcc420actggggtac cgtcgttggt catggctcgc ggacaccgtg ttgatcgtgt tccggaactt480ccacttgttt tatctaaaga ggttgaatcg cttaaaaaga caaaagatgc tgtaaaggta540ctcaaggcaa tacgggcttg gccggatatt caaaaggttt acaactcaca acgatttcgt600gcaggtaaag gtaaaatgag aaatcgaagg cgaattcaaa aacgtggccc aataataatt660tataagaagg atgatgggat atcgcgtgct tttagaaata ttccaggtgt caccttgatt720aacgtgagag ctttaaatct ccttaagctg gctccgggtg gtcatatggg acgtttagct780atttggtcag atgcagcatt tcggcagtta gacaagcttt atggcacacg aaaaacagga840agtgtgaaaa agtcgggttt ccatctccct atagctaaaa tgaccaattc ggaccttggt900agacttttgc attgccgtgc agtttcctca gtcgtaaaat ctagacgtat gcatttgcaa960cgtccaggtc caaaagtcaa gaaaaatcca ttaaaaaatc aacaagctct actgaagttg 1020aatccttttg cactcggaga aaaacacttc aaaatggctc ttgcgcgtgc cattataaaa 1080ggaaagctca agcgaaaagc agctaacaaa gttgacgaaa caccttcaaa gaaggctaaa 1140actgtaaaag ttaaataata tctttaaata tagttgtgat aatggcatta aaaaa1195<210>3<211>753<212>DNA<213>Schistosoma japonicum<400>3ttggcatctg cgtgacttaa gtcgtggttg tcgagatggg acgcagacca gccaggtgtt 60atcgatattg caaaaataaa ccttatccga agtcgaggtt ttgtcgtggt gtcccagatc120ctaagatccg aatatttgac ctcggtaaga aaaaggcaga cgttgaggaa tttcctctat180gtgtgcatat gatatctgat gaatatgaac agttatccag cgaagcactg gaagctgctc240gtatttgtgc aaacaaatat ctgctgaagc actgtgggaa agactcattc cacggtcgga300
tacgcgttca tccgttccat gtgatccgta tcaataaaat gctttcttgt gccggggctg360atcgcttgca aacaggtatg aggggtgcat ttggtaaacc tcaggggact gttgcacgag420tcgacatcgg acaagttata atgtcagttc gtgctcgtga tcaacatcaa acgcaggtta480tcgaggccct caggcgtgcg aagatgaaat tcccaggtcg acaaaagatt gctgtgtcac540gcaactgggg ctttacaaaa tggccaagag ctaatttcca tgaaatgcga gataaaggac600aactagtttc agatggtgtt ggtgcgaaat atatccccac tcatggtccc ctggaacaat660ggaaacaagc acaagcacgt ctagcaggaa tagctgtata aaaagtagta ctaaaataca720tgattggtaa agaaaaaaaa agagaaaaag gaa 753<210>4<211>583<212>DNA<213>Schistosoma japonicum<400>4ctgctatcag gtttgcacgt taattggtag catggataat cagcttccga aaccggttcc 60gattaaaaaa gttacggtta gagatttgtc tcaaataccg tctaatcaca gcacaacacc120aggcggtact ctatttagca ctactccagg tggaacccgt ataatatatg aacgtgactt180tattctaagt ttacgcaact ccccagctgc tcgtagccca cctccagata tgatattctt240gccggaaaaa gcgccaaaca gtgatgataa aacaattcat gcatgtaatg gtcataaagt300taaagattct ttgcttgatc tgcctgagtc tatctctgat catgataaca gatccaacaa360aggcgatgat gctccatttg aaatggatat ttagacagat caatgtgttc gcctattcgt420gataaccata caaggacctt ttctatatat ctttgaataa gcgattgtat ttaacatatt480cttcaccttg tttgtgcctt ttatcattct tatctatcaa acaacctgat aaaatacaca540tctgttctgt cttgtgttac atattcaaaa aaaaaaaaaa aaa 583<210>5<211>875<212>DNA<213>Schistosoma japonicum<400>5tttctaccta atctcaacac tttaacgtta gagatggggc tatttgatgt agatttgttt 60gacgacttta agcgtatgaa caaaagacag gtatactatc aagtgttaac tattgctatg120gttgtagctt ctgccgtaat gatttggaaa ctttttataa ttatcagcca tagcgaaagt180cctttggttg ttgtcttgag tggaagtatg gagcctgcgt ttcaccgggg tgatgtgcta240tacttaacta actatcccga tgagcctatc cgcgtgggtg acatcgttgt attcaaaatc300gagggaagag aaattcctat tgtccatcgt gttttaaaac tacatgaaag tgttaacgga360actatcaagt tcctcacaaa gggcgacaat aatccagtac atgaccgtgg tttgtatgct420cctggtgaag attggttgac accatctcat cttattgggc gcgcaagagg cttcattcct480tatatcggtc aggttaccat aattatgaat gaaaatcctc gtctcaagta cagtgtctta540ggagctatga gtatctactt gctactgaat agaaatcaag agtaaaaatg tttgtacaaa600tgtgtacata aacatttatt aattttgtaa ttatagtgtt tttattctaa gaaaacagaa660
tgaccactgg tcatgttcac ccaactgctt tatattgaca tctaattcat cctagccaag720tcaatttgta tgtacttttt tgttgttttg tttcgttttt ttttctaatg cttatcgtcg780atatgtccct atatgtttgt ttgaaatgaa cttcaagttg attattttgt ttcttgttgg840taagattgtt tctcagtttg gaaaaaaaaa aaaaa 875<210>6<211>1303<212>DNA<213>Schistosoma japonicum<400>6gattaactac ttacagactg catacaatcg attcttcacc tttgatctca ccgtactaat 60tcaatcctaa atggctaaag tttctggatt tggtataaca cgttcattaa ttcatgcatt120tgatccacat ggaaaacagt acaagcctac aattaaacca acaacagggt tttcagcttc180tgctgatgct gaacgattgc acagagcaat gaaaggtccc ggtactgatg aaacaactat240tattaatata ttggcacgta gaacaaatta tgaacgacaa gaaatacgtc attcatacaa300aagtatgtat aaacgggatc ttaaagatga tttaaaatcg gataccagtg gggattttcg360taaagttcta tgccaattgg ttgtggatac accttatatg ttggctaaat cattgtatta420tgcaatgaaa ggtttaggta ccaatgatcg tgtactaatt gaaatactta ctacactatg480gaatgatgaa atgagagcag tagccaatgc atataaagaa gtacttaaag acaaaggaat540agaagattcc aaccgttcat tagttacaga tatgaaaaaa gagacatgtg gtgattatga600atatgcactg cttagtttag ttcaagctga acgtgatgat atacaactat tacaattgaa660ggccatacct gagaaaggtg ttaattctgt cattaaccat gaattggctg aagctgatgc720taaagattta tacgcttcag gagctggttg cttaggtacc agagaaaaac gtattacacg780tgttatatgc aatagaacac catatcaatt atatttaaca tcagagattt atttaaaatt840gtatggtaaa tctttactgg aacatattga atcggagaca tcaggtgatt atcgtaaact900acttgtatct gtcttacggt atgcaattga tcgtgccaat cttattgctg aatggttaca960tgattcaatg gttggtttag gcacgaaaga ttatgcctta atgcgtttat taattacacg 1020ttctgagatc gatttacaag acataatgaa ggcatatgaa gttatttatg gtaaatcttt 1080attgaatgca gtaaaagatg atacatcagg tgattatcgt caaacattat gtgttctact 1140tggtgaataa attgcttaac cataacatta attactactg atatgaattc agtatcgtta 1200aagaagattt atactgtcag attgttgatg tcatcaagtt gcttcattta tcattattct 1260atatgattga attataactt tcaataaatt tcattatatt caa 1303<210>7<211>588<212>DNA<213>Schistosoma japonicum<400>7ccaaagaatg tgaagatgct atcaatcaac aaatcaatat tgaactacaa gccgcttatg 60attacatggc attcttcacc tatttcgatc gagatgatgt atcatttccg aaggcagctg120aattctttag aaaggcttca catgaagaac gtgaacatgc tgagaaatta gcaagatatc180
agaataaacg aggtggttgt gttcgatata gtgatataaa gtgtccaaag aagaccgaat240tcaatggatt agaggatgca atgaatacag ctttgtcaat ggaaaaagca gttactgatt300cattactaaa actacatgca atagcttcaa aaaacaatga tccagcattg acagatttta360ttgaaagtga atttcttcat gaacaagaag aagcaatcaa acgatttgct gattatttga420cagaaacaca acgtgttgga aaaggacttg gagaatattt attcgataaa ctaacattga480atgaataaag aaaagaaaat ttactttagt aaacatgact gaatattatt atttcattta540atacaacgtt tttattctta taatgtaaat ataataatct tttgttcc 588<210>8<211>1094<212>DNA<213>Schistosoma japonicum<400>8aatcgtatac aattgaatat attctgaaag cacacagaca cacttacaca tacaatcatg 60taaccaccgc tacatttcaa caatataaat acagtttaca tttttaaaaa aagtctattg120ataataaaag aactcaagtt gaaagtaaaa gatatactct actgaattca cttaattcac180attttcatta caactatatc aaacgaaaat atggcgagta caatggaaca atttattaca240gcttacttaa caatttatcg tgatggtgat gaggttgttg acaaaaaaga actgattgac300tattgtagaa aagagaaact cgatatgaag atggttgata catggttatc acattttgat360gttgataaag ataatcgaat tacattagaa gaattttgtc gtggtttagg cttgaaagca420aatgaaatgc gtatcgaacg aaatcatatt aaagctgtac aatctggtag agtaccaaaa480ataccagatg gtgtagaaat tattgcatca acaatgccat taccaagaca agttgaagta540acacaattat ataaagattt actaccaaaa tccggtggac atgaagctga tatgcgtaaa600gtagcaaatg atttcaaaat taaattagaa gaacgttatg aacgtgtatg gcaagtggtt660ttattgactg gttcatattg gatgaatttt tcacatgaac cattacaatc aattcaattt720aaagttgata agaatatcat tcttggcttg gcgtacacca aacaattaat agactacaag780aaaatctatt gtaaaccata attaaaacaa atcaaatgaa tactttattt agaaataaca840atctacatta aacaacttca aagttatcaa gtactattga tatgatgaat acaaacagtt900tttttttcta tttctgttct ttctttctgt actcaaccaa ccaccccccc ccgctcctac960gccctctcct gcccacttta aaaatattcc tattctttta aacatactac ttaattctta 1020gtaacaatta ttgtggaaat gttttttttg ttttctttag aaaaaaaaag gtataaattt 1080agtacagtgt attc 1094<210>9<211>2330<212>DNA<213>Schistosoma japonicum<400>9ttagcaccga tttaagcgta gcgaatttgt gtcatcttat tattatttgt tttttgataa 60gcaacttaca tcctataaaa atattataga acatttgaga atgtgggttg agaataacga120ttcaccatgc ttgggatcag ttccggttat caaagggaag tttaaagaaa tgcctaagaa180
tgctcagcgc tttgttgcca aatgggtaga aatatgtaaa ccaacaggag tgtatatatg240cgatggtagc aaagaagaaa aacaagaact tacagagaaa ttaattgaac ttggttcttt300acacaaacta ccgccatatg aaaacaacta tatcacttgt acagatccaa aagatgttgc360gcgtgttgaa tccaaaactt ggatttgctc agagcataaa aaggacactg ttcctgatgt420tgcaccgggt gttcatggtg ttcttggaca atggatatca cctgatgacc taaatactga480aattcatgat cgttatccta attgtatgga aggtcgtgta ttatatgtga ttccatttag540tatgggtccg attgggtcac cgttaagtaa agttggcatt gaattgaccg acagtatata600tgttgttcta tgtatgacaa ttatgacacg tatgcatcca aaagtatgga acgttatcga660gtctagtaaa gagtttgtca gatgtgtaca cagtgttgga tgccctacct catcaaatca720aatagtaaaa aataattggc catgtaatcc ggagaaaact ctaatttctc atgtacctaa780agaacgttta attatgagtt ttggcagcgg atatggtggg aactcattgt taggtaaaaa840atgctttgca ttacgtattg ctggttgcat tgcacgtgat gaaggctggt tagctgagca900tatgttaatt atgtcggtaa caaacccaaa aggcgaagag aaatttatag cagctgcttt960tcctagtgct tgtggtaaaa caaatatggc tatgcttgaa ccatcagtac ctggttggaa 1020agtacaatgt gttggtgatg atatagcatg gatgcgcttc gacgaacacg gtgtgttacg 1080tgcaatcaat ccagaagctg gttttttcgg tgttgctcca ggtacaaata aaaaaacgaa 1140cccaaatgca atggcaacat gtatgaaaaa cactatattc actaatattg gtcaaactaa 1200agatggtcac atcttttggg aaggcttaga agatcaatat agtcctgata cagaaatcat 1260tacatggctt ggtgatcacg ttaaactatc agataaaagt aaagatccca aagcacatcc 1320taattcacga ttttgttgtc ctgctaatca gtgtccaatt attcatccta agtgggaaga 1380tccacaaggt gtaccgattt ctgcccttat ttttggtggt cgtagaccta gtggtattcc 1440actagttatg caatcatttg actggaaaca tggtgttatg ttaggcgcgg ctttaaaatc 1500agaggcaact gctgccgctg aatttacagg taaatcggtt atgcatgatc cgatggctat 1560gcgtccattt gttggatata attttggaca ttatttagat cattggcttg gtatggagaa 1620gccgtcacgt aaaatgccat taatatttca tgttaattgg ttcagattaa atgaacatgg 1680taaatttgta tggcccggtt tcggacataa tatacgtgtt atcgattgga ttcttcgacg 1740agttgatggg gaagataatg gtgttcattc cccgattggt attttgccta aaaaagattc 1800aatcaatttc gatggtttaa atattgattg ggatgaaaac ttctcattgc caaaagatta 1860tcttctagaa gatgttgatg aaacaatcaa atatttacat gaacaggttg gcaaagattt 1920accagttgtt atacagaaag aattgatcaa tcaacgtgaa cgtattcagt ctgaacttta 1980attggtgaaa ttatctgtta tgctttgtga tttcctactg caatatctaa tgaattgaaa 2040tcaatcaaaa ttgtttgata catttcaaat catttctgtt ctgttattgt tgttattctt 2100tgacttcatt gcacttcgca ttaaatgatc gttagttgac atgataactt gctttttaat 2160actactatta atagtactat tattattatt attattaatt atcctgacga atatgattca 2220aatgttttaa atacaaataa acaacaaata attgactgat tatttctctt tataagttgt 2280taatgttaat tttccaattg attaaaagat aagtcaattt tttcttcttt 2330<210>10<211>751<212>DNA<213>Schistosoma japonicum<400>10ttttaagatc ggtcacatgc ctcaaaacga acacatcgaa cttcatcgca gacgccatgg 60
atatcggttt gactattttg agaggaaacg aaagaaagag gctcgtgaac cacatgagag120aagtcgtaaa gccaaaaaac ttcgaggatt gaaagcgaaa attgccaaca aagaaagatt180taaggaaaaa gttcagatga agaaaaccct gcgtatgcat gaattaaaag gaaaaaaccc240aaatgtcgaa aatgtcggac gatctggcga agtgcctgca tatcttttag atcgtggtga300acagatttca gccaaaattt tgtctaacac tgttaaacag aaaagagctg agaaagcggg360aaaatgggag gtaccgttac caaaagtaag aagtgtctct gaagcggaag catttaaagt420tgtacgaaca ggaaagtcta aacgtaaggc gtggaagcgt ctggttacta aaatgtgctt480tgttggcgat acgtttaccc gaaaaccccc taaattcgaa cgttttattc ggccaatggg540tttacgtgtg aagaaagcaa atgtcactca tccggaactt aaaactactt ttcagctccc600gataattggg gtaaaaaaaa atccaagttc gcctatgtac actaccttag gtgtaataac660taaaggtaca attattgagg tcaatgtcag tgatcttggt ttggtcacac cgagtggcaa720agttgtgtgg ggtaaatatg ctcaagtaac c 751<210>11<211>2480<212>DNA<213>Schistosoma japonicum<400>11atcgagacaa gcgtcagtag aaaagtgtaa ccatgaagtt gagtttggtc ccagattgag 60catatatgca acagtaagaa gtacaatgta ggaaatgttg tgtctagaga tacaccgact120gctaaagagt cgcatacgag tatgaagcag ctatccggtg ccagctccta gtctccacta180cttctctaac tcacaccagt tcgtggtatc aaattaaatg gtcaaactta tttgtactgt240gacttgaatt tcttgctcct cgtcgtagat aatcgtcaaa ttgtgattgg ctgacagaca300actgaaaaca atggaatttc cgctgtgata ctgttctcaa tagttgtcgc ttgaaatcat360gtgttcgtga ttagttcaat gaagctgatc ttatccactt cagatcgatg gtgaaatttc420atcgaatttt caccaaaaga gtctgtaacg gtaaccaaac gtccagacgc cgggcgctga480agatacaact agacaggctt cccaaataag aaccaattga gccaaaccaa aagaaatgaa540tcccaaataa tgtatttatt gacctgtcta gcaatattgt tctatgcaat aaagtataaa600cgtattatta caaaccttca atattatagg cacccagtct gaaaacaaat ggttaacagt660ccaaaacaaa ggtgcgagcc aaaatggcgg gagtgaagat aaacaaacaa gatacatagt720gaacataaca aataagtcaa gtttggaaga gcatgacaag aataattgac agatttatat780aatcattaag tttaccgcaa cacgtactct acagcaaact ctggagtaag ctaccacaaa840atattcgcac tactaacttt atcctaatgt tcaaacgact gatcaccata tacctagaat900ctatagactt atacaaattt gttaacgact taccacttaa cgttgaatca atcagcaatg960gcttattaag gctctaaaac tacaaacact acgttgtagt ggcaaaaaag cgatgaaaag 1020aaaattctag tttgatattg attattttga tcccactaat taaaattcgc ttttcttttt 1080ctatttcata tattcttcta tatcattgtc tccccgagtt tgtgatctgc tctcgtatgg 1140aattggtctc ctttaagcaa cttggctcca aaccatgggt aagaaggaaa ttaacgcatc 1200aataactcac cgttctagac caacccacat aaattggctc tttcaaccta atatcaaata 1260ctcagtcctt acccgtttct ggatagtctc tatgatccat tctgcttacc acagccattt 1320tctgcggcac cctgggtttg tcggtccata ctacgatgtc ggttgaacgt gggacttcta 1380actctgcatc ctacaaaatg tttctcactc atggcgggtc acctatttct tattttcatg 1440atgttccact ttttgctgat gcgactaaca actgttacaa tatgattgtt gaaatccctc 1500
gctggacaaa tgcaaaaatg gagatttgca aggaagaact tatgaacccc atcaaacatg 1560atgtcaaaaa taataaattg cgctatatat ataatgtatt tccacataag ggttatatct 1620ggaattatgg agctttacct caaacatggg aagatccaag ctatgtagat gaggatacta 1680aagcgaaagg agacaacgat cctattgatg tttgtgaaat tggttcaaaa atttggcctt 1740caggttcagt aatacctgtc aaagtattag gtatattggg aatgatagat gaaggggaaa 1800ctgattggaa agttatcgct atcaatgtgg ccgacccgat ggctgaaaaa cttaatgaca 1860ttcttgatgt agatgcacat atgccgggat ttttgaaggc tacacgagat tggtttaagt 1920actacaaagt tcctgcaggg aaacctgaaa attcgttcgc ttttaacggg gaattcaaaa 1980acaaggaatt cgcagtaaaa attatttcca aaacacatga acattggcaa aaactaatat 2040ctaccaaagt cgaagcaggt cctattatac gagcaaatgt tactgtgaag ggaagtcctt 2100atatggtatc gaaagaagac tttatcgatg ctttacaaaa gcacgaagat tttaaacgtg 2160gaagtgagcc gacagatcaa gctattgaac aatggcattt ttgtaatacg aatatttaac 2220tccgcttcct cactggttgg ctatgttatc ttttaatcac tgttttgtgc attaatcaca 2280tggtgcattc tacacaatcc ctgcatatca aactttgcac atttttctag tatttttgaa 2340agcagaaagt atattattgt aatttttacg ttttgttgtt gttgttgtaa cctttagtat 2400ttacgtatgt gggtgtaggt ttatgaatat ttcgaattcg aacatttaat cgatataaat 2460atacatgttt ttttaaaaat 2480<210>12<211>708<212>DNA<213>Schistosoma japonicum<400>12cacacgcact taatcgtgcg ggtcgtatcg gcgtacttgt tttgtggaga tttgggtttt 60atcacataat atggaagcgt gtgcaacaat tataaaagaa aatattgatt tagtctcaca120actcaatgag aaagaatttc ccgcccttcc gaaaacagga ctgtcccgtc ccgtcaaagt180tttgggtgct ttgatacctt cctacaaagc taaaactaca gaagttttag aaataccttt240cgaggaaagg cgattcctta ataatgaaaa cgacaatctt tcagctggca aaagatacaa300tgttcagagc aaaatctgta tggaaatcgc taaggataca aatgtgaaga tgaatttaaa360cagctctaaa aatcatatct taacagtcat tgtttcaggg gaaccaagca gagttgctga420ggcaaagaga agagtgattg cagaattgca acatcaggaa actgttcgga tctctgttcc480tgttgagtgt cgaggttatt taattggtcg aaaaggtgag cgactgcaag aactagaatc540atctacgatg acacgaatca gcattcctcc tcacaatgcc cttgatccag atgtcgttat600tgttactggt cccaaaagag ggattttgga agcagaagaa ctgatatatg aaatcgttcg660caagcaatcc caacaagcgt ttgaacggtt gtcgatacca aaaattta 708<210>13<211>637<212>DNA<213>Schistosoma japonicum<400>13
ttgctatcgt tcgtattaat tctgctctca ggatcctgac ttcgatctaa cgttaggatg 60tcacttacag agagtaacga ctggttggcg ctttatgaaa gcgtatcacg tatcaaaaac120agcacaaatc ctgaattggg tcagggagaa tctgatgaga agccttttac tagggcggaa180atatcgtata tcaataaatt actgaactcc aagctatttg aaacgcatga tttagatttc240gaggttctaa gatctgaccc tgatcatcct cttcattctg ttcgcacttt tcaagaattg300aacttaaaag aaccactgtt aaaaggtatt gcagcaatgg gattttataa gccgtcaact360atccaggagc gagctctgag cagtttaata tctgacaacc cacaaaatat gattgcacag420tctcagtctg gtactggaaa gacagcaaca tttcttcttg ctatgttgtc acgtattaga480acagatgttc actactgcca gtgtctttgt atggcaccta cacgcgagtt ggccttgcag540atagaatcag ttggtcgaca aatggcacag tttatgacgg atgtttcttt tgctacagca600gtccgttctt cacgtgccaa agtcgattcg gataggt 637<210>14<211>605<212>DNA<213>Schistosoma japonicum<400>14caagtattaa actgaagttt aagaatggca agaatcggta aaggtattca atcatgtcta 60tcttgggttc atgaaagaga tggtcgtgta ccatcgaatg caattgacac aggtcatgct120gtttacatag gtcgaatgta ccattctggt gatttaattc ctggcaaagt tgtaccacat180ttgggtaaag catatgctag ttatggtggg agagaatatg aatttgattc ttatgaagta240ttatgtgata caaaattacc gcatatttct aaacaatgtt acagatggga acgacacagt300aacggatatg ttcctaaata tgctgttgtt ggtggtataa cttcttctaa tgaaccactt360tatatagcac gtgagcatat tgaaggagaa cgtgtagttg gaaagataca tgaaggtcat420gaatgtgctt atttcccatt tggtggtgaa gaacgaaaaa tgcaacatta tgatgttctt480gtattgatta aataatttga aacagagtga caatacggaa ctgaatgttt aattttgttt540ttacttttct tcaccttatg gtttaaataa aacaaatttt atttataaaa aaaaaaaaaa600aaaaa605<210>15<211>1437<212>DNA<213>Schistosoma japonicum<400>15taagaaaatc caaaccttct gttgagtata ttgaatcagt cgatggctgc aaacagttta 60tcgataatgc tacaatagcc gtccttggat ttatcaagga tacggactct ctagatttaa120cagatttcga aaaagtggct gatgaactgg acgatgcgga ttttgcagta gccaactcca180gtgatattct gaatgagtat ggtattacac agacaccaag aattgtcttg ttcaaaaatt240ttgacgaaaa tcgtgttgat tatactggaa aaacattgga aaacctgaag cacttcgtcc300aggtggaaag tgttccactg gtctcagaat tttctcagaa gaccgcaggg gttgtgttcg360gaagtccggt tcaaaaacat atcgtattct tcctttccaa atctgcggac cacttagatt420
atgttaacag actgaccgaa gttgcgaagc agttcaaaag caagcttcac gttatatacg480tcgatgttga tgttgaaaac aatttacgtg ttttggaatt tttcggactt tcaaaaaatg540atgccccaac ttaccgaatt atcgagttgg gtgaagaaac aactaaatac aaaccggaca600ccgatgatta ttctgtttct ggcatgtccg attttgtaca aagagcactt gacggaaaag660ttaaaccttt ccttatgagt gaagaaattc caactgatca aacaggtgct gttagggtcc720ttgtcgggaa aaattacaat gatgttgtta gggacagatc aaaggatgtc tttgttaaat780tgtatgctcc ttggtgcggt cattgcaagg ctcttgctcc agtctggaac gaattgggtg840aagcttttaa agacgcagat gtagtgattg caaaaatgga tgctacagtc aatgaagtag900aagatctcag agttactagt tttccaactc tgaagtttta tcccaaaaat tcggatgagg960ttatcgatta tacgggcgat agatcttttg aagctttgaa gaagtttgtg gagtcaggtg 1020gcaaatctag tgaaacaact aaagaagagg atcaaataaa ggatgaactc tgaacttact 1080ttgtagaacg atctactttg ctttttctca ctgctctcac ttcccttggc tttttgaatc 1140ttttttctcc attacctgtc aataaaaatt gggcaatagt ttaacttgcc cattaactag 1200ttatttgtag ccatatgtat ttgtttttaa ggcgctatct tacagtgcat ttccatgtga 1260atgttcctca agttttttta taacctacgt gcaccataaa tgttttttct ttctaaatat 1320ttttaaattg tgttaaatta aattcatatt cacttgtaaa tttaactttt ggaactatgg 1380aaacatttgt ttgcatcgaa tgtacaagaa agtttaccaa aaaattgtgt tgtttag 1437<210>16<211>580<212>DNA<213>Schistosoma japonicum<400>16tcaggtgaaa cgtgatattt gctaaaaatg ggtcgtatgc acagtggtgg taaagggatt 60tcgagaagtg ctctacctta tagacgatct gtaccatcat ggcaaaaaat gacggcagat120gaggtgaagg aacagatata caaactagcg aaaaaaggat tatcaccatc ccaaattggt180gttatattgc gtgacagttt tggggtcgct caggttaggt ggctagcagg aaataaaatt240ctccgcattc tgaagtgcaa aggattagca ccagatattc cagaggattt gtatcaattg300gttaaaaagg ccgttgcgat tcgaaaacat ctaggcagaa atgccaagga taaggatagc360aaatttagat tgattctagt tgaaagccgt atccagcgtt tagcacgcta ctataaaaga420aaaagagtcc taccaccgaa ctggaagtat gacagctcca cggcatcaac actgattgct480tgattttttg taattacatt taaagtatat ggtctgtaag ctctttttga agtaaagatg540ctatatgact cgttcttaaa aaaaaaaaaa aaaaaaaaac 580<210>17<211>2674<212>DNA<213>Schistosoma japonicum<400>17tctctacaaa ctaataattt tgatagaata ttgacatcat caccaactat ttccctgata 60ctattcatat tttcattgta ttctgtatta aatagtatca gggaaatagt tggtgatgat120
gtcaatatac aggctaaata cgatgaagaa agtgaagaag ctagtaattt aagaaaccaa180gtatctaagt ttaacgctga tatcgctgca ttaaaatcaa aatttgaacg tgaacttatg240agtaaaacag aggaattcga agaaatgaag cgaaaattga caatgagaat cactgaactt300gaagatgttg cagagagaga acgattaaaa gcggtatcat tagaaaaact caaaacaaaa360ttaacactgg aaattaaaga tctacagtct gaaatagaaa gtctttcatt agagaatggt420gaattaattc gtcgtgctaa atctgctgaa tcattagctt ctgaactaca acgtcgtgta480gatgagctta caattgaagt gaacacattg acatcacaaa acaatcaatt agaaagtgaa540aatatgcgat taaagagttt agtaaatgat ttaactgata aaaacaatgc attggaacgt600gaaaatcgtc aaatgaacga tcaagtaaag gaattaaaga gttcacttcg agatgctaat660cgtcgtctta ctgatttaga agctttaagg tctcaattag aagctgaaag agataatctt720gcttcagctt tacatgatgc cgaagaagct ttacgtgata tggaccagaa gtatcaagca780tcacaagctg cactgaatca tttaaaatct gaaatggaac agagacttag agaaagagat840gaagaattag aaagtctaag aaagagtaca actagaacaa ttgaagagtt gactgttaca900attactgaaa tggaagtgaa gtataaatca gaattgtcac gtttaaagaa acgttatgaa960tcaagcatag ctgatttaga aattcaactt gatgctacaa acaaagcaaa tgcaaatctt 1020atgaaagaaa ataagaattt agcacaacgt gttaaagatt tggaaacatt tttagatgat 1080gaacgtcgtc tacgtgaagc agcagaaaat aatctacaaa tcactgaaca taagcgtata 1140cagctggcaa atgaagttga agaattgcgt agtgcaatgg aaaatttgga acgtttgcgt 1200aaacatgctg aaacagaact tgaggaaact cagtcacgtg ttagtgaatt aactattcaa 1260gttaatacat tgagcaatga taaacgtcgt cttgaaggtg atattggtgt aatgcaagct 1320gatatggatg atgctatcaa tgctaaacaa gctgctgaag acagagcaac tagattgaac 1380aatgaagttt tgagattagc tgatgaatta cgccaagaac aagaaaatta taaacatgct 1440gaagctttga gaaaacaact tgaaattgaa attcgtgaaa tcactgtgaa attagaagaa 1500gctgaagcat ttgctactcg tgaaggtcgt cgtatggttc aaaaactgca ggcccgtgta 1560cgtgaacttg aagcagaatt cgatggtgaa tcacgtagat gtaaagatgc tctagcacaa 1620gcacgtaaat ttgagcgtca gtacaaagaa ttacagacac aagctgaaga tgatcgtcgt 1680atggttctag aacttcaaga tttattagat aaaactcaaa tgaaaatgaa agcatacaag 1740cgtcaattag aagagatgga ggaagtttct caaattacaa tgaataaata tcgtaaagca 1800caacaacaaa ttgaagaagc agaacatcgt gcagatatgg ctgaacgtac agtgacggta 1860cgtcgtgttg gtccaggtgg acgtgctgtg tcggtagcac gtgaattatc tgtcacttca 1920aatcgaggaa tgagagcaac aagtatgatg taaagcactt aaacacacat acatacacac 1980atatatacat atttgttcgt tattctttcg ctttcctcat ctttcctttg catttttatt 2040gaggaaatta tcatcaaatg ataactaata caaataacaa tcaacttatt gctacacaat 2100gtgctttgct gtactcactc tctctcttct tagttttctc tatgtgttct ttatctatgt 2160taattccttt aatgaagagt gaagaaacag tttcacaaca tgaaactaat tattgttacc 2220attactacta ctactactac cactaccaca aataacaata acaataatga taatagtagt 2280aatgataata gcattattat tattattact attatatttg ttccaaaatt agcttacaaa 2340acctaggcat gattttattt tgcacaattt cttttttttt ttacaaatat tatttctccc 2400cccccctctc cgaccctcca ctaccgaaaa aaacattgtt tcccttctta ctaaaacatg 2460aatagcatca tcaattattc tattggtgtc acctgcctac ttacaaaaac taaattactt 2520tcattccatg ttacaatttt ctggcttatt tgtggcttta tagtgtaaaa ccaaaaaaaa 2580cataaatccg tagattcata aaatggttta aaaaaaaaaa aaaaaaaccc cggggggggc 2640cccggaccaa tccccccaaa gggagcgata caat 2674
<210>18<211>1899<212>DNA<213>Schistosoma japonicum<400>18ccgacggcgt gagtcagtga taatcaaccg gtgacaatgt tacgagctct aagttcactt 60cgtggtagtt tggttccagc tagacaaaaa gttgttcaaa ggttttatgc aaaggaagtg120aagttcgggg ctgacgcaag atcagctatg ttgattggtg ttgatgtact ggcggatgct180gttgcagtca ccatgggccc taaaggtcgt aacgtaataa tagaaagctc gtggaaatct240ccaaaaatca caaaagatgg ggttactgtt gccaaaggta ttgagctgaa ggataagttc300cagaatattg gggctaagtt agtacaggac gttgctaaca acaccaacga agaggctggg360gatggtacta caacagccac tgttttagcg cgagctatag ctaaagaggg ttttgaaaag420ataagtaaag gcgctaatcc tatcgagttc cgaagaggtg ttatgcttgc tgttgatgct480gttgtcaaag agctcaaatc tttttcgaag caaatatcca caccagaaga gatagcacaa540gttgcgacaa tatccgctaa cggtgacaag gctattggcg acctaatcgc ttcagctatg600aagagggttg ggaatgatgg cacgataact gttaaggatg gcaaaactct tcacgatgag660ttggagttta tcgaaggtat gaaattcgac cgtggttata tctctccata ttttatcaac720actgagaaag gagcccgatg tgaattccaa gatgccttca tattgttttc tgaaaaaaag780ataaacagca ttcagacatt attaccagct ctcgaactat gccaccaaca aaaacggccg840ctgctgatta tcgccaagga tgttgagggt gaagctctta cagcacttgt actaaatcgc900ttaaaactgg gcttacaggt ctgtgcagtc aaggcaccca aggttttcgg ggataatcgc960gaaaaataca ttaaaagata tggctgtagc aacaggggta tagtgttcgg gacgaagcag 1020atatttgtcc aagttagagg atgtacaact gcaagatttg ggacgtgttt cagaagctgt 1080aataacaaaa gacgattgtt tactaatgcg aggtcgtgga aacaaaatgg acatcgacaa 1140acgtatcgcc acaaattaaa gatgaaatgg aagcctccaa cagcgagtat gaaaaaagaa 1200aaaatgcacg aacgcttggc caagttgtca aacggcgtcg cagtaattaa ggttggtgga 1260agtagtgaag tagaagttag tgagaaaaag gatcgatata ctgatgcact caatgcaacg 1320agagcagcaa ttgaggaagg catagtgcct ggtggtggca ctgctttgct gcgatgcatt 1380cctgtcctgg atacgttatc taccaagaac gaagatcagc gaacaggtgt gcggattgtt 1440ctacgtgcac tttcaactcc gtgctatacc attgcacata acgcaggtgt caacgcctct 1500gttgtcgttg aaaaagtcaa aggtatgagc cagaatatgg gttacgatgc tcaaaatgat 1560gtttacgttg atatgatcga ggctgggatt attgacccaa ctaaggtcgt cagaactgca 1620ttggttgatg cagctggtgt tgcttcctta ctcacaactg cagaaaccgt ggtgacggat 1680ttaccaaaag aagagaccgg aggcaacgca gcaggaatgg gtgggatggg aggtatgggt 1740ggaatgggag gcatgatgta aacactgcct gctcttaatg catttcgctg tttctaaatc 1800gtagttgagt aatcttaata gatgttccat ttgttcaaag tccctgattt acccccaaat 1860atacaaatga tttcatacag ttaaaaaaaa aaaaaaaaa 1899<210>19<211>1350<212>DNA<213>Schistosoma japonicum
<400>19aaaagaaaca gggattatcc aacgatgatg tagttgtcag atttaatgaa tctgaagcta 60agaatactga aacgcaggat atttttaagg agcctcactg taaaagatgt gatgaaccat120ttactgaaga tgaaaatatt gtctctgtaa aagaaggtac ctatcaccca acatgttttg180tatgtgctca atgttttcaa ccattaccta acaaagaatt ttacgaattt gaaggtcggc240gatattgcaa atatgatttt caagttcttt ttgcgccatt ttgttctaag tgtggtgact300ttatcatgtc caaagtggtt aaagcaataa acagaagttg gcatccagaa tgtttaatat360gtgatgaatg tggagtccaa cttgttagca aaggatttca gagacataat aatagaattc420tatgtaaaca ttgttggtct gttataaacc gagcattaac tgggtgttat atttgtcaaa480catgtaatag atcaattgaa ttgaataagc atatcaaatt tatgggtgat tttcatcatc540cacatcattt tcattgttac gattgtggtg aagaacttgg ctcagatgca cgtgaacata600gtggtgaact atattgtctc agatgttttg gacgtaccgg gatatctatt tgctcagctt660gcagacgtcc aatagacggt cgtatagttt gggcacttgg taaagtttgg catgttgaac720attttgtgtg ccatcactgt gaaataccat ttatgggtag tcgattttat gaatggcaag780gacatgccta ttgtttacta cattatcaag ctaagattgg aagtatttgt aataaatgca840caaaacctgt tactgggata ttggcaaaat tcactaataa aatttattgt cctgaacact900ttctctgctc gttgtgtgat agacagttag atgaaaaatc aaaactttat gaaatcgatt960tgaaaccagt atgtaaaaac tgttatgata agttaccaat acaatggaag aaaattttgg 1020cgaaaattca tcgtacagat agacaaaaat ggaatgattg ataattgtct gtgttgtcaa 1080tgtggttttt cttttgttta taactaatga aacgaaaaaa aatatttttt ttaaataaaa 1140aataacgaat gaaaaagtat ctatttttag aaatgaaagc tgcaaaacaa acatgttatc 1200gatgttgtat tatgagtacg atttttcagt atttccaaaa ttatcttttt tatttcagta 1260gtatttcgaa tgcaaaataa atttatagat ttcattttca tttttcacta tttaccaatt 1320gatcagtatt caaggttaaa cagtaaataa1350<210>20<211>1500<212>DNA<213>Schistosoma japonicum<400>20attgaaggac acatcttcgg tcatagtata cgtgatgggt ttgtcgaagc tcagtatctc 60cgatgtagat ttaaaaggca aacgtgtgct cattagggtt gattttaacg tccctatgaa120agatggaaaa gtcactaata cccaacggat tgcggctgct ataccaacta ttaaatatgc180cttggataag ggagctaaaa gcgtagtcct gatgtctcac ttaggtagac cagatgggaa240taaagttgaa aaatattcgc tgaaacccgt ttgctctgaa gtttccaagt tgctaggcaa300agaagttact ttccttagtg actgcgttgg tcctgatgtc attaacgcgt gttctaatcc360tgctcctggc agcgtcttct tacttgaaaa tttacgcttt catgtagaag aggaaggaaa420aggtgtctca ctatctggag ataaaacaaa ggccactgct gatcaaatca aagctttcag480tgagagttta acaaaacttg gtgatgttta tgtcaatgat gcctttggaa cagcacatcg540tgctcatgcc tctatggtcg gctgccaact gccacaaaaa gcttgtggat ttctcatgaa600taaagagttg acatattttg ctaaagctct ggaaaatcct gaacgtcctt tcttagctat660tttgggcggc gctaaggtta gcgataagat ccagttgatc aataatatgc tggacaaggt720taacgaattg atcattgggg gcggcatggc gtatactttc cttaagcaaa tacacaatat780
gaatatagga gctagtttat ttgacgcacc aggtgcagaa attgttcaga aggtaatgga840gactgcaaag gctaaacacg tggcaattca cttacctgtt gattttgtaa ctgctgataa900atttgctgaa gatgctaaca ccggaacagc aactatagaa tctggaattc cagatggttg960gatggggctg gatattggtc cgaaaaccat agaggaattc cgtaaggtga taagtcgtgc 1020caaaacaata gtttggaatg gtccgatggg tgtttttgaa atggataagt ttgctgcagg 1080gacaaaagca gcaatggatg cggtagttga agctactaaa aacggtgcta caactattat 1140tggtggcggt gatacagcga cttgttgtgc caagtggaac acagaagata aagtcagtca 1200tgtcagtacc ggtggtggtg ctagccttga acttttagaa ggtaaacaac tgccaggagt 1260cgttgcactt acggatgctc actaatctat cagactatga tttcctagtt aattgtcatt 1320agtactcctc ttactttcat aatgttcaaa ttatttactt gttattataa cacgtttgtt 1380cttgatttgt cgtatatttt tctctaccta atttcaatac gtgtagatct atttctgcca 1440tatgtgcatt ttcaggcaat aaaaactcat agatttttgc aaaaaaaaaa aaaaaaaaaa 1500<210>21<211>1577<212>DNA<213>Schistosoma japonicum<400>21tatcgtgtta tcttattctg ccatatcatt cttgactccc tacttattaa agatgcctta 60acggaatgta gaaataaaga tgctgtccgc ttttttccgg gagtatggcc tcagccaact120tttaaccact tttcaggcta tttgaatgga tccaacaaca atattagatt gcattattgg180cttgtagagg ctgtcagaag ccctaaaact gctccgttaa ttttgtggct aaatggcgga240ccaggatgtt catcgatgga agggtttttc agtgaaaacg gtccttacaa tatgatacgg300ggaacaaatt tggtagaaaa cccctattcc tggaacaaat tggcaaatgt cttgtatttg360gaatcgccag caggtgttgg gttctcatac gctgtagata ataatataac tacagatgat420gattatacag cactgaacaa ttattatgct ttgttgcatt ttctgaaaag gtttccagag480tataagggac gagaatttta tataacaggc gaaagttatg ctggtgttta tgtacctttg540ttagcattac acgtcattaa atcacaacaa ttcaatttga aaggtatagc tgtaggcaac600ggtttaacta attataaatt caatgataat tctttgatat acttcatcaa ataccacgga660ttagtgagtg aaagaatgtg gaatgatcta cttaaacatt gttgccacag tcagtattac720agtcactgtt ttttcacgga tgctacatca gttaaatgtc aaagtctagt taaatatatt780ttagataatg caactgctgg attaaatata tacaatttat atgattcctg tggtaatatc840aataattcta tggatcagaa acttgaaaat ctttatcact cgtcggatat gaaatcattt900tcccaaccat ttttacacag tgattttggt aatcttttta gaagtaataa attctttcaa960gaaaaacgtg agaaaataaa tgaactccga aagaaaattg gaacgagact agttttaccc 1020tgtgatgatg acaatattat tggtagatat ttggatctac cgtttgtacg tgaatcaatt 1080catgtgagag aagataaacc aaaaacatgg gaagtatgca gtgatagcgt aatgtcagtg 1140tataaacgga attatcaaga tttatctcct cagtatagaa atatccttaa gtcaaagatt 1200ccaatactta tatataatgg agatgttgat atggcttgca actttatagg agatgattgg 1260tttgtgaata atttgaaatt cgattctcat aaccaatatc aacgttggat ttataaatca 1320gaggatggta aagagcaaat tggaggattt tggaaatctt ttatacataa gagcgtgaac 1380ttgatcttta caactgttag aggtgcaggt catatggtac ctagagataa gccagctgct 1440atgtttcact tgattcagtc atttatacag gcaaaaagtt tatagactga cagtaacatt 1500
ttcaagaatg tactttatta attactacag tttccttaaa taaatatctt aattttatga 1560aaaaaaaaaa aaaaaaa 1577<210>22<211>822<212>DNA<213>Schistosoma japonicum<400>22tttttttttg gttgtttgca tgataatggt tccaggagct acaaacaagc ttgaaaatac 60aaaagttgca tcagattctg atagcgactc agacgactct gcgccagaat tagaaaattc120taaagaacag ggagatgcca aagtgtcaca tatagcggac gaattactca gtcgatgcaa180gcagtcaaga ggcgaaaaga aagctcggaa agccatgtca aaactaggac tgaagttggt240gcagggtgtt tgccgcgtaa ctatacggaa agcgaaaagt attatgtttg tgatcaatag300ggcagaggtc tataaatcat ctgcctcgga cacatatata atatttggtg aggccaaagt360ggaagatata tcagcacaag cacagattgc cgcagcggaa aaacttcgta gtcaagctat420gggaggtact gatctaggga cacctggaag cctatcgaaa gatgcagttt catcagtctc480taagactgta attgcagaag agtccgaaga tgatgaagag cctgatgcta gtggactgag540tgagaaagat attgaactta tcatgcaaca agctggcgtg tcccgaagca aagctattcg600ggcattgcgt gagaataacg atgacatggt caaatgcgat tatgtcactt acaggatagg660tggttggctt caacttataa ttcgtggtaa cttggattga cctacaagta cttctaaaac720tttcagtaac tacacacttg ttacttctca tacactcatt atcatccaag aaatttttct780acattggaaa aaaaaaaaaa aaaaacaaaa aaaaaaaaaa aa 822<210>23<211>1076<212>DNA<213>Schistosoma japonicum<400>23gtaattcatt gtagccaaac actcattcat tattaatcga gttcattgta ttcacaatag 60tgaagaaata aataattgat cattgataat aaaagaagaa aatgcatctc agtttaattc120tattacaatt atgcacattt taccttgtac tggcacaaga agaatatcat aaagttgaag180aagaaccgga tgaagatcca tgtgaaaatg taaaatgtaa attgggtgaa atatgtaaag240aaggtaaatg caattgtatg gatagttgtc catcggaatg gaatagttac aatagacaat300tgtgcgttga tggtgttaca tatagacatg aatgtgatct atggagaaat caatgttatt360gtcgttcagg tgatacaaga tgtggaagtg atcaattcag tagtcataga gcaaatgaaa420attcagcaat taaatattat gatgaatgtc gtgatcttag tggtctatgc gattgggatc480aaaatgaaag taccttctca cttcgacttg gaatgtggtt tcaagagtta ttacgtcaaa540aatggtcatc tgcttcttgg tcaattgatg atgatagtct tcttcgtcca atggatgcaa600aagcaagaag tactacaagt aaattagttt cacaaagttc atcacatgaa agagtcaatg660gagctgtgat cagttattgg ttttgtgaac ttgatagaag gatagaggat cattacaaca720agatgattta aattatttat atcaattatt gttaccttca acaccatgtt tagaagtatt780
cattaatcga tgttctggtt ctggtggaat atcatatgat caatggcata attgttttga840tattcctcaa gaagaacgtg ttcaatgtaa cgaattcaaa taaatcgaac tgatctattg900aatattgaac attgaaatgt aatagaacta atgaattcac tgaaaatttg ttttaatttt960cgaaaaaaaa acaaacaaaa catgaaaatc tataaaagaa acaaaaaaaa ttgaatgtaa 1020tttatcaaac aaaggtatta aaaataaaaa ttaatcttaa aaaaaaaaaa aaaaaa 1076<210>24<211>342<212>PRT<213>Schistosoma japonicum<400>24MPLTSRSPHDSKTLLAKYYDLPQPDDKVQVMYVWIDGSGENLRCKTMTLPDEPQAPEDCP60MWNFDGSSTGQAEGSNSDVYLKPCAMFRDPFRGGKNKLVLCETFNYDKKPHVSNHRYLCN120LVMEKAKIHEPWFGIEQEYALLDIDGYPLQWPKNGFPPPQGPYYCSVGAGKALGRDIPEA180LYRACMYAGVKICGSNAEVMPSQWEFQVGPCEGVSAGDHLWMARFILHRVAEDFGVIVTL240DPKPMPGNWNGSGAHTNYSTLAMRNPKSGLQAIEDAVEKLSHKHTEHIKCYDPKHGLDNQ300RRLTGSHETSSINDFSSGVANRGSSIRIPRQVAENGCGYSGR 342<210>25<211>375<212>PRT<213>Schistosoma japonicum<400>25MAVTRPIVNVYGVNGTSTGATIKMPAVFTTPLRLDLLNFVHDQMRKNSRQPYAVSTKAGH60QTSAESWGTGRAVARIPRVRGGGTHRSGQGAYGNMCRGGHMYAPTKTMRRWHRKINIQQR120RYAICSAIAATGVPSLVMARGHRVDRVPELPLVLSKEVESLKKTKDAVKVLKAIRAWPDI180QKVYNSQRFRAGKGKMRNRRRIQKRGPIIIYKKDDGISRAFRNIPGVTLINVRALNLLKL240APGGHMGRLAIWSDAAFRQLDKLYGTRKTGSVKKSGFHLPIAKMTNSDLGRLLHCRAVSS300VVKSRRMHLQRPGPKVKKNPLKNQQALLKLNPFALGEKHFKMALARAIIKGKLKRKAANK360VDETPSKKAKTVKVK 375<210>26<211>221<212>PRT<213>Schistosoma japonicum<400>26MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKKADVEEFPLCVHMISDEYEQL60SSEALEAARICANKYLLKHCGKDSFHGRIRVHPFHVIRINKMLSCAGADRLQTGMRGAFG120KPQGTVARVDIGQVIMSVRARDQHQTQVIEALRRAKMKFPGRQKIAVSRNWGFTKWPRAN180
FHEMRDKGQLVSDGVGAKYIPTHGPLEQWKQAQARLAGIAV 221<210>27<211>120<212>PRT<213>Schistosoma japonicum<400>27MDNQLPKPVPIKKVTVRDLSQIPSNHSTTPGGTLFSTTPGGTRIIYERDFILSLRNSPAA60RSPPPDMIFLPEKAPNSDDKTIHACNGHKVKDSLLDLPESISDHDNRSNKGDDAPFEMDI120<210>28<211>183<212>PRT<213>Schistosoma japonicum<400>28MGLFDVDLFDDFKRMNKRQVYYQVLTIAMVVASAVMIWKLFIIISHSESPLVVVLSGSME60PAFHRGDVLYLTNYPDEPIRVGDIVVFKIEGREIPIVHRVLKLHESVNGTIKFLTKGDNN120PVHDRGLYAPGEDWLTPSHLIGRARGFIPYIGQVTIIMNENPRLKYSVLGAMSIYLLLNR180NQE 183<210>29<211>359<212>PRT<213>Schistosoma japonicum<400>29MAKVSGFGITRSLIHAFDPHGKQYKPTIKPTTGFSASADAERLHRAMKGPGTDETTIINI60LARRTNYERQEIRHSYKSMYKRDLKDDLKSDTSGDFRKVLCQLVVDTPYMLAKSLYYAMK120GLGTNDRVLIEILTTLWNDEMRAVANAYKEVLKDKGIEDSNRSLVTDMKKETCGDYEYAL180LSLVQAERDDIQLLQLKAIPEKGVNSVINHELAEADAKDLYASGAGCLGTREKRITRVIC240NRTPYQLYLTSEIYLKLYGKSLLEHIESETSGDYRKLLVSVLRYAIDRANLIAEWLHDSM300VGLGTKDYALMRLLITRSEIDLQDIMKAYEVIYGKSLLNAVKDDTSGDYRQTLCVLLGE 359<210>30<211>140<212>PRT<213>Schistosoma japonicum<400>30
MAFFTYFDRDDVSFPKAAEFFRKASHEEREHAEKLARYQNKRGGCVRYSDIKCPKKTEFN60GLEDAMNTALSMEKAVTDSLLKLHAIASKNNDPALTDFIESEFLHEQEEAIKRFADYLTE120TQRVGKGLGEYLFDKLTLNE140<210>31<211>196<212>PRT<213>Schistosoma japonicum<400>31MASTMEQFITAYLTIYRDGDEVVDKKELIDYCRKEKLDMKMVDTWLSHFDVDKDNRITLE60EFCRGLGLKANEMRIERNHIKAVQSGRVPKIPDGVEIIASTMPLPRQVEVTQLYKDLLPK120SGGHEADMRKVANDFKIKLEERYERVWQVVLLTGSYWMNFSHEPLQSIQFKVDKNIILGL180AYTKQLIDYKKIYCKP196<210>32<211>626<212>PRT<213>Schistosoma japonicum<400>32MWVENNDSPCLGSVPVIKGKFKEMPKNAQRFVAKWVEICKPTGVYICDGSKEEKQELTEK60LIELGSLHKLPPYENNYITCTDPKDVARVESKTWICSEHKKDTVPDVAPGVHGVLGQWIS120PDDLNTEIHDRYPNCMEGRVLYVIPFSMGPIGSPLSKVGIELTDSIYVVLCMTIMTRMHP180KVWNVIESSKEFVRCVHSVGCPTSSNQIVKNNWPCNPEKTLISHVPKERLIMSFGSGYGG240NSLLGKKCFALRIAGCIARDEGWLAEHMLIMSVTNPKGEEKFIAAAFPSACGKTNMAMLE300PSVPGWKVQCVGDDIAWMRFDEHGVLRAINPEAGFFGVAPGTNKKTNPNAMATCMKNTIF360TNIGQTKDGHIFWEGLEDQYSPDTEIITWLGDHVKLSDKSKDPKAHPNSRFCCPANQCPI420IHPKWEDPQGVPISALIFGGRRPSGIPLVMQSFDWKHGVMLGAALKSEATAAAEFTGKSV480MHDPMAMRPFVGYNFGHYLDHWLGMEKPSRKMPLIFHVNWFRLNEHGKFVWPGFGHNIRV540IDWILRRVDGEDNGVHSPIGILPKKDSINFDGLNIDWDENFSLPKDYLLEDVDETIKYLH600EQVGKDLPVVIQKELINQRERIQSEL 626<210>33<211>250<212>PRT<213>Schistosoma japonicum<400>33FKIGHMPQNEHIELHRRRHGYRFDYFERKRKKEAREPHERSRKAKKLRGLKAKIANKERF60KEKVQMKKTLRMHELKGKNPNVENVGRSGEVPAYLLDRGEQISAKILSNTVKQKRAEKAG120KWEVPLPKVRSVSEAEAFKVVRTGKSKRKAWKRLVTKMCFVGDTFTRKPPKFERFIRPMG180
LRVKKANVTHPELKTTFQLPIIGVKKNPSSPMYTTLGVITKGTIIEVNVSDLGLVTPSGK240VVWGKYAQVT 250<210>34<211>287<212>PRT<213>Schistosoma japonicum<400>34MSVERGTSNSASYKMFLTHGGSPISYFHDVPLFADATNNCYNMIVEIPRWTNAKMEICKE60ELMNPIKHDVKNNKLRYIYNVFPHKGYIWNYGALPQTWEDPSYVDEDTKAKGDNDPIDVC120EIGSKIWPSGSVIPVKVLGILGMIDEGETDWKVIAINVADPMAEKLNDILDVDAHMPGFL180KATRDWFKYYKVPAGKPENSFAFNGEFKNKEFAVKIISKTHEHWQKLISTKVEAGPIIRA240NVTVKGSPYMVSKEDFIDALQKHEDFKRGSEPTDQAIEQWHFCNTNI 287<210>35<211>212<212>PRT<213>Schistosoma japonicum<400>35MEACATIIKENIDLVSQLNEKEFPALPKTGLSRPVKVLGALIPSYKAKTTEVLEIPFEER60RFLNNENDNLSAGKRYNVQSKICMEIAKDTNVKMNLNSSKNHILTVIVSGEPSRVAEAKR120RVIAELQHQETVRISVPVECRGYLIGRKGERLQELESSTMTRISIPPHNALDPDVVIVTG180PKRGILEAEELIYEIVRKQSQQAFERLSIPKI212<210>36<211>193<212>PRT<213>Schistosoma japonicum<400>36MSLTESNDWLALYESVSRIKNSTNPELGQGESDEKPFTRAEISYINKLLNSKLFETHDLD60FEVLRSDPDHPLHSVRTFQELNLKEPLLKGIAAMGFYKPSTIQERALSSLISDNPQNMIA120QSQSGTGKTATFLLAMLSRIRTDVHYCQCLCMAPTRELALQIESVGRQMAQFMTDVSFAT180AVRSSRAKVDSDR 193<210>37<211>156<212>PRT<213>Schistosoma japonicum
<400>37MARIGKGIQSCLSWVHERDGRVPSNAIDTGHAVYIGRMYHSGDLIPGKVVPHLGKAYASY60GGREYEFDSYEVLCDTKLPHISKQCYRWERHSNGYVPKYAVVGGITSSNEPLYIAREHIE120GERVVGKIHEGHECAYFPFGGEERKMQHYDVLVLIK156<210>38<211>356<212>PRT<213>Schistosoma japonicum<400>38RKSKPSVEYIESVDGCKQFIDNATIAVLGFIKDTDSLDLTDFEKVADELDDADFAVANSS60DILNEYGITQTPRIVLFKNFDENRVDYTGKTLENLKHFVQVESVPLVSEFSQKTAGVVFG120SPVQKHIVFFLSKSADHLDYVNRLTEVAKQFKSKLHVIYVDVDVENNLRVLEFFGLSKND180APTYRIIELGEETTKYKPDTDDYSVSGMSDFVQRALDGKVKPFLMSEEIPTDQTGAVRVL240VGKNYNDVVRDRSKDVFVKLYAPWCGHCKALAPVWNELGEAFKDADVVIAKMDATVNEVE300DLRVTSFPTLKFYPKNSDEVIDYTGDRSFEALKKFVESGGKSSETTKEEDQIKDEL356<210>39<211>151<212>PRT<213>Schistosoma japonicum<400>39MGRMHSGGKGISRSALPYRRSVPSWQKMTADEVKEQIYKLAKKGLSPSQIGVILRDSFGV60AQVRWLAGNKILRILKCKGLAPDIPEDLYQLVKKAVAIRKHLGRNAKDKDSKFRLILVES120RIQRLARYYKRKRVLPPNWKYDSSTASTLIA 151<210>40<211>650<212>PRT<213>Schistosoma japonicum<400>40SLQTNNFDRILTSSPTISLILFIFSLYSVLNSIREIVGDDVNIQAKYDEESEEASNLRNQ60VSKFNADIAALKSKFERELMSKTEEFEEMKRKLTMRITELEDVAERERLKAVSLEKLKTK120LTLEIKDLQSEIESLSLENGELIRRAKSAESLASELQRRVDELTIEVNTLTSQNNQLESE180NMRLKSLVNDLTDKNNALERENRQMNDQVKELKSSLRDANRRLTDLEALRSQLEAERDNL240ASALHDAEEALRDMDQKYQASQAALNHLKSEMEQRLRERDEELESLRKSTTRTIEELTVT300ITEMEVKYKSELSRLKKRYESSIADLEIQLDATNKANANLMKENKNLAQRVKDLETFLDD360ERRLREAAENNLQITEHKRIQLANEVEELRSAMENLERLRKHAETELEETQSRVSELTIQ420
VNTLSNDKRRLEGDIGVMQADMDDAINAKQAAEDRATRLNNEVLRLADELRQEQENYKHA480EALRKQLEIEIREITVKLEEAEAFATREGRRMVQKLQARVRELEAEFDGESRRCKDALAQ540ARKFERQYKELQTQAEDDRRMVLELQDLLDKTQMKMKAYKRQLEEMEEVSQITMNKYRKA600QQQIEEAEHRADMAERTVTVRRVGPGGRAVSVARELSVTSNRGMRATSMM 650<210>41<211>574<212>PRT<213>Schistosoma japonicum<400>41MLRALSSLRGSLVPARQKVVQRFYAKEVKFGADARSAMLIGVDVLADAVAVTMGPKGRNV60IIESSWKSPKITKDGVTVAKGIELKDKFQNIGAKLVQDVANNTNEEAGDGTTTATVLARA120IAKEGFEKISKGANPIEFRRGVMLAVDAVVKELKSFSKQISTPEEIAQVATISANGDKAI180GDLIASAMKRVGNDGTITVKDGKTLHDELEFIEGMKFDRGYISPYFINTEKGARCEFQDA240FILFSEKKINSIQTLLPALELCHQQKRPLLIIAKDVEGEALTALVLNRLKLGLQVCAVKA300PKVFGDNREKYIKRYGCSNRGIVFGTKQIFVQVRGCTTARFGTCFRSCNNKRRLFTNARS360WKQNGHRQTYRHKLKMKWKPPTASMKKEKMHERLAKLSNGVAVIKVGGSSEVEVSEKKDR420YTDALNATRAAIEEGIVPGGGTALLRCIPVLDTLSTKNEDQRTGVRIVLRALSTPCYTIA480HNAGVNASVVVEKVKGMSQNMGYDAQNDVYVDMIEAGIIDPTKVVRTALVDAAGVASLLT540TAETVVTDLPKEETGGNAAGMGGMGGMGGMGGMM 574<210>42<211>352<212>PRT<213>Schistosoma japonicum<400>42KKQGLSNDDVVVRFNESEAKNTETQDIFKEPHCKRCDEPFTEDENIVSVKEGTYHPTCFV60CAQCFQPLPNKEFYEFEGRRYCKYDFQVLFAPFCSKCGDFIMSKVVKAINRSWHPECLIC120DECGVQLVSKGFQRHNNRILCKHCWSVINRALTGCYICQTCNRSIELNKHIKFMGDFHHP180HHFHCYDCGEELGSDAREHSGELYCLRCFGRTGISICSACRRPIDGRIVWALGKVWHVEH240FVCHHCEIPFMGSRFYEWQGHAYCLLHYQAKIGSICNKCTKPVTGILAKFTNKIYCPEHF300LCSLCDRQLDEKSKLYEIDLKPVCKNCYDKLPIQWKKILAKIHRTDRQKWND352<210>43<211>416<212>PRT<213>Schistosoma japonicum<400>43MGLSKLSISDVDLKGKRVLIRVDFNVPMKDGKVTNTQRIAAAIPTIKYALDKGAKSVVLM60
SHLGRPDGNKVEKYSLKPVCSEVSKLLGKEVTFLSDCVGPDVINACSNPAPGSVFLLENL120RFHVEEEGKGVSLSGDKTKATADQIKAFSESLTKLGDVYVNDAFGTAHRAHASMVGCQLP180QKACGFLMNKELTYFAKALENPERPFLAILGGAKVSDKIQLINNMLDKVNELIIGGGMAY240TFLKQIHNMNIGASLFDAPGAEIVQKVMETAKAKHVAIHLPVDFVTADKFAEDANTGTAT300IESGIPDGWMGLDIGPKTIEEFRKVISRAKTIVWNGPMGVFEMDKFAAGTKAAMDAVVEA360TKNGATTIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKQLPGVVALTDAH416<210>44<211>409<212>PRT<213>Schistosoma japonicum<400>44MEGFFSENGPYNMIRGTNLVENPYSWNKLANVLYLESPAGVGFSYAVDNNITTDDDYTAL60NNYYALLHFLKRFPEYKGREFYITGESYAGVYVPLLALHVIKSQQFNLKGIAVGNGLTNY120KFNDNSLIYFIKYHGLVSERMWNDLLKHCCHSQYYSHCFFTDATSVKCQSLVKYILDNAT180AGLNIYNLYDSCGNINNSMDQKLENLYHSSDMKSFSQPFLHSDFGNLFRSNKFFQEKREK240INELRKKIGTRLVLPCDDDNIIGRYLDLPFVRESIHVREDKPKTWEVCSDSVMSVYKRNY300QDLSPQYRNILKSKIPILIYNGDVDMACNFIGDDWFVNNLKFDSHNQYQRWIYKSEDGKE360QIGGFWKSFIHKSVNLIFTTVRGAGHMVPRDKPAAMFHLIQSFIQAKSL 409<210>45<211>226<212>PRT<213>Schistosoma japonicum<400>45MIMVPGATNKLENTKVASDSDSDSDDSAPELENSKEQGDAKVSHIADELLSRCKQSRGEK60KARKAMSKLGLKLVQGVCRVTIRKAKSIMFVINRAEVYKSSASDTYIIFGEAKVEDISAQ120AQIAAAEKLRSQAMGGTDLGTPGSLSKDAVSSVSKTVIAEESEDDEEPDASGLSEKDIEL180IMQQAGVSRSKAIRALRENNDDMVKCDYVTYRIGGWLQLIIRGNLD 226<210>46<211>209<212>PRT<213>Schistosoma japonicum<400>46MHLSLILLQLCTFYLVLAQEEYHKVEEEPDEDPCENVKCKLGEICKEGKCNCMDSCPSEW60NSYNRQLCVDGVTYRHECDLWRNQCYCRSGDTRCGSDQFSSHRANENSAIKYYDECRDLS120GLCDWDQNESTFSLRLGMWFQELLRQKWSSASWSIDDDSLLRPMDAKARSTTSKLVSQSS180SHERVNGAVISYWFCELDRRIEDHYNKMI 209
權利要求
1.一種DNA序列，其編碼表達于日本血吸蟲表皮的蛋白質，其特征在于它具有序列表中SEQ ID NO.1～SEQ ID NO.23所述序列中任一序列的堿基序列；或者它是在中度嚴緊條件下與序列表中SEQ ID NO.1～SEQ ID NO.23所示的序列中任一條序列雜交的序列；或者它是與序列表中SEQ ID NO.1～SEQ ID NO.23所示的序列中任一條序列有至少70％同源性的序列。
2.一種氨基酸序列，其編碼表達于日本血吸蟲表皮的蛋白質，其特征在于它具有序列表中SEQ ID NO.24～SEQ ID NO.46所述序列中任一序列的氨基酸序列；或者它是與序列表中SEQ ID NO.24～SEQ ID NO.46所示的序列中任一條序列有至少70％同源性的序列；或者它是包含有序列表中SEQ IDNO.24～SEQ ID NO.46所示的序列中任一條序列的氨基酸序列。
3.一種從日本血吸蟲中分離獲得日本血吸蟲表皮蛋白質的制備方法。
4.根據(jù)權利要求2所述的氨基酸序列在制備血吸蟲免疫蛋白的免疫原方面的應用。
5.根據(jù)權利要求2所述的氨基酸序列在篩選化學和其他種類藥物的潛在的藥物的作用靶點。
6.根據(jù)權利要求2所述的氨基酸序列在制備抗血吸蟲抗體的特異性血吸蟲抗原方面的應用。
7.根據(jù)權利要求2所述的氨基酸序列在制備血清學診斷方面需要的蛋白質方面的應用。
8.根據(jù)權利要求1所述的核苷酸序列在制備用于基因治療的核苷酸序列方面的應用。
全文摘要
本發(fā)明公開了一類在日本血吸蟲表皮上表達的表皮蛋白，尤其公開了所述表皮蛋白的編碼核苷酸和編碼氨基酸。通過對其進行大規(guī)模的蛋白質組學研究，認為它們可能具有藥物作用靶點和候選疫苗的作用。血吸蟲病的流行給人民身體健康和經(jīng)濟發(fā)展都造成巨大的損失，因此，防治血吸蟲病、開發(fā)研制治療血吸蟲病和抗血吸蟲的藥物具有巨大的社會和經(jīng)濟效益。
文檔編號A61P33/00GK1986801SQ20051011172
公開日2007年6月27日 申請日期2005年12月20日 優(yōu)先權日2005年12月20日
發(fā)明者劉鋒, 韓澤廣, 王志勤, 胡薇, 馮正 申請人:上海人類基因組研究中心, 中國疾病預防控制中心寄生蟲病預防控制所