專(zhuān)利名稱(chēng)：日本血吸蟲(chóng)表皮基因、其編碼蛋白及應(yīng)用的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及生物基因領(lǐng)域，尤其涉及日本血吸蟲(chóng)表皮蛋白質(zhì)的氨基酸序列及其基因核酸序列；此外，本發(fā)明還涉及日本血吸蟲(chóng)表皮基因、蛋白的應(yīng)用。
背景技術(shù)：
血吸蟲(chóng)病由血吸蟲(chóng)的感染引起，在世界上有76個(gè)國(guó)家，受感染者約1.5億。致病的血吸蟲(chóng)有3種，既日本血吸蟲(chóng)(Schistosoma japonicum)，曼氏血吸蟲(chóng)(S.mansoni)和埃及血吸蟲(chóng)(S.haematobium)。在我國(guó)流行的是日本血吸蟲(chóng)。血吸蟲(chóng)病的流行給人民身體健康和經(jīng)濟(jì)發(fā)展都造成巨大的損失，因此，防治血吸蟲(chóng)病、開(kāi)發(fā)研制治療血吸蟲(chóng)病和抗血吸蟲(chóng)的藥物具有巨大的社會(huì)和經(jīng)濟(jì)效益。
研制新的治療血吸蟲(chóng)病和抗血吸蟲(chóng)的藥物，首先必須找到合適的藥物作用靶點(diǎn)。血吸蟲(chóng)的表皮是宿主和血吸蟲(chóng)相互作用的界面。而組成血吸蟲(chóng)表皮的蛋白質(zhì)暴露在這個(gè)界面上，對(duì)小分子的化學(xué)藥物和大分子的抗體藥物而言都是最理想的藥物作用靶點(diǎn)。已經(jīng)有研究表明治療血吸蟲(chóng)的化學(xué)藥物——吡喹酮的作用靶點(diǎn)就是存在于血吸蟲(chóng)的表皮。另外一些已知的血吸蟲(chóng)表皮蛋白，例如GST28，paramyosin，已經(jīng)用于疫苗研究和應(yīng)用。通過(guò)大規(guī)模的蛋白質(zhì)組學(xué)研究，發(fā)現(xiàn)了日本血吸蟲(chóng)表皮蛋白質(zhì)，這些蛋白質(zhì)，都是可能的藥物作用靶點(diǎn)和候選疫苗。
在本發(fā)明之前，還沒(méi)有出現(xiàn)涉及本發(fā)明的日本血吸蟲(chóng)表皮蛋白的公開(kāi)報(bào)道。

發(fā)明內(nèi)容
本發(fā)明要解決的技術(shù)問(wèn)題之一是提供一類(lèi)日本血吸蟲(chóng)表皮蛋白，及其基因的核酸序列。
本發(fā)明要解決的技術(shù)問(wèn)題之二是提供一類(lèi)日本血吸蟲(chóng)病防治疫苗，該疫苗以日本血吸蟲(chóng)表皮蛋白為免疫原。
本發(fā)明要解決的技術(shù)問(wèn)題之三是提供一類(lèi)與日本血吸蟲(chóng)表皮蛋白結(jié)合的抗體。
本發(fā)明要解決的技術(shù)問(wèn)題之四是提供日本血吸蟲(chóng)表皮蛋白在制備預(yù)防和治療日本血吸蟲(chóng)病的藥物中的應(yīng)用。
為了解決上述技術(shù)問(wèn)題，本發(fā)明通過(guò)如下技術(shù)方案實(shí)現(xiàn)在本發(fā)明的一個(gè)方面，提供了一類(lèi)日本血吸蟲(chóng)表皮蛋白，包括
(a)具有SEQ ID No.22～SEQ ID No.42所示氨基酸序列的蛋白；(b)由(a)的蛋白發(fā)生一個(gè)或幾個(gè)氨基酸置換、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
較佳地，所述蛋白是具有SEQ ID No.22～SEQ ID No.42氨基酸序列的蛋白。
在本發(fā)明的另一方面，提供了一類(lèi)編碼日本血吸蟲(chóng)表皮蛋白的基因，包括(a)SEQ ID No.1～SEQ ID No.21所示的核苷酸序列；(b)SEQ ID No.1～SEQ ID No.21所示的核苷酸序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.21所示的核苷酸序列中每條序列有至少70％同源性、且編碼相同功能蛋白質(zhì)的核苷酸序列。
較佳地，所述基因具有SEQ ID No.1～SEQ ID No.21所示的核苷酸序列。
在本發(fā)明的另一方面，還提供了一類(lèi)日本血吸蟲(chóng)病防治疫苗，該疫苗以日本血吸蟲(chóng)表皮蛋白為免疫原。
在本發(fā)明的另一方面，還提供了一類(lèi)與日本血吸蟲(chóng)表皮蛋白結(jié)合的抗體。
此外，本發(fā)明還提供了日本血吸蟲(chóng)表皮蛋白在制備預(yù)防和治療日本血吸蟲(chóng)病的藥物中的應(yīng)用。
在本發(fā)明中，術(shù)語(yǔ)“日本血吸蟲(chóng)表皮蛋白”指具有日本血吸蟲(chóng)表皮蛋白活性的SEQ ID No.22～SEQ ID No.42所示氨基酸序列的蛋白質(zhì)。該術(shù)語(yǔ)還包括具有與日本血吸蟲(chóng)表皮蛋白質(zhì)相同功能的、SEQ ID No.22～SEQ ID No.42序列的變異形式。這些變異形式包括(但并不限于)若干個(gè)(通常為1-50個(gè)，較佳地1-30個(gè)，更佳地1-20個(gè)，最佳地1-10個(gè))氨基酸的缺失、插入和/或取代，以及在C末端和/或N末端添加一個(gè)或數(shù)個(gè)(通常為20個(gè)以?xún)?nèi)，較佳地為10個(gè)以?xún)?nèi)，更佳地為5個(gè)以?xún)?nèi))氨基酸。例如，在本領(lǐng)域中，用性能相近或相似的氨基酸進(jìn)行取代時(shí)，通常不會(huì)改變蛋白質(zhì)的功能。又比如，在C末端和/或N末端添加一個(gè)或數(shù)個(gè)氨基酸通常也不會(huì)改變蛋白質(zhì)的功能。該術(shù)語(yǔ)還包括日本血吸蟲(chóng)表皮蛋白的活性片段和活性衍生物。
在本發(fā)明中，術(shù)語(yǔ)“編碼日本血吸蟲(chóng)表皮蛋白的基因序列”指編碼具有日本血吸蟲(chóng)表皮蛋白活性的核苷酸序列，如SEQ ID No.1～SEQ ID No.21所示的核苷酸序列及其簡(jiǎn)并序列。該簡(jiǎn)并序列是指，位于SEQ ID No.1～SEQ ID No.21序列中，有一個(gè)或多個(gè)密碼子被編碼相同氨基酸的簡(jiǎn)并密碼子所取代后而產(chǎn)生的序列。由于密碼子的簡(jiǎn)并性，所以與SEQ ID No.1～SEQ ID No.21所示核苷酸序列的同源性低至約70％的簡(jiǎn)并序列也能編碼出SEQ ID No.22～SEQ ID No.42所示的氨基酸序列。
在本發(fā)明中，可以使用一系列本領(lǐng)域已知的方法來(lái)制備針對(duì)日本血吸蟲(chóng)表皮蛋白的抗體。例如，將提純的日本血吸蟲(chóng)表皮蛋白產(chǎn)物或它的抗原片段注射入動(dòng)物體內(nèi)以產(chǎn)生多克隆抗體。同樣，表達(dá)日本血吸蟲(chóng)表皮蛋白或它的抗原片段的細(xì)胞也可以用來(lái)對(duì)動(dòng)物致免疫而產(chǎn)生抗體。本發(fā)明制備的抗體也可以是單克隆抗體，這些單克隆抗體可用雜交瘤技術(shù)制備。
應(yīng)用本發(fā)明的日本血吸蟲(chóng)表皮蛋白，可研究血吸蟲(chóng)治療藥物的作用靶點(diǎn)，從而在開(kāi)發(fā)研制預(yù)防或治療血吸蟲(chóng)病的藥物的過(guò)程中發(fā)揮重要作用。


圖1是用免疫熒光抗體原位雜交實(shí)驗(yàn)驗(yàn)證本發(fā)明的日本血吸蟲(chóng)表皮蛋白的圖。
具體實(shí)施例方式
下面結(jié)合附圖和實(shí)施例對(duì)本發(fā)明作進(jìn)一步詳細(xì)的說(shuō)明。
以下實(shí)施例僅用于說(shuō)明本發(fā)明而不用于限制本發(fā)明的范圍。實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法，通常按照常規(guī)條件，例如Sambrook等人，分子克隆實(shí)驗(yàn)室手冊(cè)(New YorkCold Spring Harbor Laboratory Press，1989)中所述的條件，或按照制造廠商所建議的條件。
實(shí)施例1日本血吸蟲(chóng)表皮蛋白的分離和鑒定實(shí)驗(yàn)。
1.日本血吸蟲(chóng)表皮的分離純化血吸蟲(chóng)表皮的純化按照文獻(xiàn)報(bào)道的Triton-x-100提取方法進(jìn)行。表皮蛋白從血吸蟲(chóng)雄蟲(chóng)、雌蟲(chóng)、雌雄混合成蟲(chóng)和童蟲(chóng)分離。蟲(chóng)體用Krebs’-Ringer’s，Tris-馬來(lái)酸鹽-緩沖鹽液(KRTM)洗3次，再用KRTM/0.2％Triton X-100溶液懸浮。這個(gè)液體和蟲(chóng)體的混和物置于冰水浴中，在搖床上搖動(dòng)10分鐘。然后，裝有液體和蟲(chóng)體的混和物的試管在渦旋混合器上混旋幾次，然后再放回冰水浴中，靜置使蟲(chóng)體下沉。用吸管吸出上部液體，放置到另一個(gè)新管中。這個(gè)試管在離心機(jī)上用2500g的離心力，離心15分鐘。得到的沉淀就是血吸蟲(chóng)的表皮。Krebs’-Ringer’s溶液的配方是0.15M NaCl，6.2mM KCl，1.2mM Na2HPO4，1.2mM MgSO4，10mM Glucose and 20mM Tris-Hepes，pH7.4。Tris maleate緩沖溶液的配方是0.2M Tris and 0.2M Maleicanhyclride，pH7.4。
2.日本血吸蟲(chóng)表皮蛋白的電泳分離日本血吸蟲(chóng)表皮用裂解劑裂解，蛋白溶液用SDS-聚丙烯酰胺凝膠電泳分離，條帶切割后再做第二維的在線(xiàn)毛細(xì)液相色譜分離以及質(zhì)譜鑒定。軟件分析得到鑒定蛋白。分離好的血吸蟲(chóng)表皮，用SDS-聚丙烯酰胺凝膠電泳(SDS-PAGE)緩沖液懸浮，用超聲波促進(jìn)組織細(xì)胞裂解和蛋白溶解，超聲波裂解方法為超聲1秒，暫停3秒，總時(shí)間2分鐘。并用100℃加熱5分鐘以使蛋白質(zhì)變性。然后這些表皮蛋白混合物用SDS-PAGE分離，分離方法按照普通的SDS凝膠電泳方法進(jìn)行。膠上電泳泳道用刀切割下來(lái)，再用刀均等分割成8-12份。接下來(lái)做蛋白的膠內(nèi)酶解。
3.日本血吸蟲(chóng)表皮蛋白的膠內(nèi)酶解過(guò)程膠內(nèi)酶解參照文獻(xiàn)的方法進(jìn)行。蛋白膠塊用刀切碎，每個(gè)小塊大小約為1立方毫米。加入100mM NH4HCO3，洗2次；轉(zhuǎn)入到試管中；加入10mM二硫蘇糖醇(dithio-threitol，DTT)/100mM NH4HCO3，56度放置反應(yīng)1小時(shí)；冷卻到室溫，離心吸去DTT；加入等體積的55mM碘乙酰胺iodoacetamide/100mM NH4HCO3，室溫/黑暗反應(yīng)45分鐘；吸去iodoacetamide，加入100mM NH4HCO3，洗2次；去掉NH4HCO3，加入乙腈縮膠；去掉乙腈，真空抽干；加入100mM NH4HCO3，膠膨脹，去掉NH4HCO3，加入乙腈縮膠；去掉乙腈，抽干；加入含Trypsin的消化緩沖液(50mM NH4HCO3/12.5ng/ul trypsin)冰上放置45min；去掉多余酶液，加入50mM NH4HCO3，以剛覆蓋膠為準(zhǔn)；37度，過(guò)夜酶切；酶切結(jié)束后，從試管中吸出上清液到新管子中，膠中加入60％ACN/0.5％HAC，在水浴中超聲處理15min，再重復(fù)一次；吸出上清液到新管子中，在原管子中再加入60％ACN/0.5％HAC，37度40min，吸出上清液，與前面的上清液合并，真空抽氣去ACN。
4.日本血吸蟲(chóng)表皮蛋白酶解肽段的在線(xiàn)色譜分離和質(zhì)譜鑒定酶解產(chǎn)生的肽混合物，用真空將溶液體積抽減到30μl。之后，每個(gè)組分的肽混合物上樣到一個(gè)反相捕獲柱(C18，5μm，300，300μm i.d×5mm，LC Packings)，在20μl/min的流速下脫鹽。然后這個(gè)捕獲柱和一個(gè)分析用的75μm×150mm C18柱(LCPackings)相連接，肽混合物就在200nl/min的流速下，從這個(gè)柱子上洗脫進(jìn)入裝有Protana NanoES的電噴霧離子源的QSTAR pulseri中。Agilent 1100毛細(xì)液相系統(tǒng)(Agilent Technologies)用來(lái)提供流動(dòng)相A(0.5％乙酸/水)和流動(dòng)相B(0.5％乙酸/乙腈)，線(xiàn)性梯度是60分鐘內(nèi)5％B到50％B，30分鐘內(nèi)50％B到90％B，然后是在90％B停留15分鐘。在分析柱后通過(guò)一個(gè)不銹鋼的兩通(Valco Instrument)連接一個(gè)10μm直徑的PicoTip納噴霧頭(New Objective)，加上2500-3000伏的噴霧電壓，以獲得穩(wěn)定的噴霧。MS/MS串聯(lián)質(zhì)譜譜圖通過(guò)信息依賴(lài)的采集(IDA)和任務(wù)-循環(huán)增強(qiáng)來(lái)記錄。在每個(gè)測(cè)定掃描中，選取3-6個(gè)信號(hào)最強(qiáng)、帶2到3個(gè)電荷的離子，用滾動(dòng)碰撞能量打碎，以獲得串聯(lián)質(zhì)譜的信息。
5.日本血吸蟲(chóng)表皮蛋白的軟件搜索鑒定用牛胰酶的自裂解片段如([M+H]+)1153.5741，2163.0569和2274.1600作為內(nèi)標(biāo)，或者以預(yù)先數(shù)據(jù)庫(kù)搜索得到的得分高的肽段的分子量，來(lái)校正所有的質(zhì)譜譜圖。質(zhì)譜搜索用的數(shù)據(jù)庫(kù)，包括從8420個(gè)從EST cluster預(yù)測(cè)得到的血吸蟲(chóng)蛋白質(zhì)序列，以及可能的污染蛋白質(zhì)序列如牛胰酶的序列、宿主蛋白污染如人蛋白質(zhì)序列、小鼠蛋白質(zhì)序列、大鼠蛋白質(zhì)序列、兔子蛋白質(zhì)序列和角蛋白序列等。搜索軟件是MASCOT(http://www.matrixscience.com/，Matrix Science)。搜索參數(shù)為最大漏切點(diǎn)，3個(gè)；monoisotopic；肽電荷數(shù)，+1/+2/+3；修飾，半胱氨酸carbamoylmethylation和methionine氧化。搜索結(jié)果合并后，用下列標(biāo)準(zhǔn)進(jìn)行過(guò)濾篩選(1)對(duì)每一個(gè)串聯(lián)質(zhì)譜譜圖的搜索結(jié)果列表，只提取排位在第一個(gè)的結(jié)果，其他的則丟棄；(2)搜索得到的肽段中如果有KR，KK，RK，RR等序列，則這個(gè)肽段丟棄；(3)搜索得到的肽段，長(zhǎng)度在8個(gè)氨基酸以上的保留，小于8個(gè)氨基酸的丟棄；(4)重復(fù)打到的肽段，只計(jì)算其中分值最高的，把這些肽段分值加起來(lái)，做為蛋白的得分；(5)蛋白的得分超過(guò)60，就認(rèn)為是可信的結(jié)果。低分的蛋白，手工檢查后保留或者丟棄。匹配到宿主或者牛胰酶、角蛋白的肽段，則丟棄。
實(shí)施例2日本血吸蟲(chóng)表皮蛋白的生物信息學(xué)分析和功能注釋。
1.同源性和相似性分析。日本血吸蟲(chóng)表皮蛋白序列對(duì)GenBank的非冗余數(shù)據(jù)庫(kù)(Non-redundant，NR)做BLASTP比對(duì)；日本血吸蟲(chóng)表皮蛋白序列對(duì)Swissprot數(shù)據(jù)庫(kù)做BLASTP同源性比對(duì)。根據(jù)比對(duì)結(jié)果得到的與已知其他物種或已知的血吸蟲(chóng)蛋白的序列相似性，做出日本血吸蟲(chóng)表皮蛋白的功能注釋。
2.文獻(xiàn)查檢。根據(jù)已經(jīng)發(fā)表的文獻(xiàn)報(bào)道，發(fā)現(xiàn)鑒定得到的50個(gè)表皮蛋白在蟲(chóng)體上的位置，有一些的確在血吸蟲(chóng)表皮上。而其他則是新發(fā)現(xiàn)的表皮蛋白。以下表皮分離和質(zhì)譜鑒定得到的蛋白，根據(jù)文獻(xiàn)報(bào)道，就是已知的表皮蛋白，且有些蛋白已經(jīng)進(jìn)行了疫苗和抗原研究，如paramyosin，tropomyosin，myosin，dynein light chain1，22.6kDa tegument membrane-associated antigen，tegumental antigen Sm20，nitric oxide synthase 1(NOS1)，leucine aminopeptidase，14-3-3 proteins，SnaK，cathepsin B-like cysteine protease precursor，21.7 kDa antigen，F(xiàn)K506-bindingprotein50(immunophilin)。
實(shí)施例3日本血吸蟲(chóng)表皮蛋白的實(shí)驗(yàn)驗(yàn)證。
為了進(jìn)一步驗(yàn)證用蛋白質(zhì)組學(xué)手段鑒定得到的血吸蟲(chóng)表皮蛋白，選取了一個(gè)Immunophilin(SjFKBP50，GenBank登錄號(hào)為AY815389)做免疫熒光抗體原位雜交實(shí)驗(yàn)，對(duì)Immunophilin蛋白作定位分析。
實(shí)驗(yàn)方法血吸蟲(chóng)成蟲(chóng)用冷凍包埋劑(OCT)完全包埋，切成5μm厚的切片。用抗Immunophilin的抗體覆蓋切片，在37℃反應(yīng)60分鐘。該抗體是用重組表達(dá)的Immunophilin免疫小鼠制備的單克隆抗體。隨后切片用PBS洗滌，再加入FITC-偶聯(lián)的兔抗鼠免疫球蛋白的抗體(1∶40稀釋?zhuān)煤?.5mg/ml的Evans藍(lán)的PBS稀釋)，置濕盒中37℃反應(yīng)60分鐘。染色結(jié)果用Leica DM-RB型熒光顯微鏡觀察并拍照，得圖1。從圖1可以看出，Immunophilin確實(shí)分布在血吸蟲(chóng)成蟲(chóng)的表皮上，T指tegument(表皮)，G指gut(腸道)，ST指subtegument(下表皮)，綠色熒光部分是Immunophilin的信號(hào)。免疫熒光抗體原位雜交實(shí)驗(yàn)結(jié)果表明，前面表皮分離和質(zhì)譜鑒定的結(jié)果是可信的。
序 列 表<110>上海人類(lèi)基因組研究中心<120>日本血吸蟲(chóng)表皮基因、其編碼蛋白及應(yīng)用<130>NP-10259<160>42<170>PatentIn version3.3<210>1<211>1745<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>1catttaacat aagctactat gagtctcgta actcctgcct tcccagtctt taatctggtg 60gattcagcat atgatgcagt tttgctacta aacgacgatg tagaacattt acctaatgct120ttacagctgg ctttgaatgc gttaaaggaa ttttctgagg tcaatcctaa attttcagat180gaagtcagta ttattccttt cccacaacat ccatcaaaaa gattaatatt tagtccaacc240ggtcaattaa atacagatga agcagacatt cgtaatgtgt atgatgctgc tgtgaatggc300atgttaaaat tgttgaaaat aggttgtaaa tcaccgctat tatgttgcgg atcattcgta360tcagctccaa aagatgctca atggtccgag cgtaattgtt tattattgaa tgctgtatta420ggagcatatc atgcattata tacgcctctt gaagttcgtg aaatgcttcc agagaagttt480cctaaagcca tccgatttgg agtgatggaa gctaacgact ccttactaaa tatcgccaaa540gctattgaag aagggagaac gttagcacgt gatattggtg ggtcagatcc tgaacgtatg600gcagccccga aaattgctga atatttaaaa aatgctttag atggtcttac aggtgttaca660atcactatta ataaagttga tgctaaaaag tatccgttaa tggcagctgt aaatagagct720gcatcagttg tacctcgtca tgacggtaga gttgtccata ttgactataa accatcaagc780tccaaagaaa ttgatacatc attatttctg gtcggtaaag gtataacttt tgatactggt840ggtgctgatg ttaaagctgg tgggattatg gctggaatgc atagagataa atgtggtgct900gcagcaatag ccggtttaat gaaaacgatt ggattattac aaccaactgg actaagtgta960tccgcagctt tagcgtttgt gcgaaatagt gttggtgagg aatcatatgt tgctgatgag 1020attatcattg cgcgttctgg acaacgggta cgtataggca atacagacgc tgaaggtaga 1080acggttatgg cagatttatt atgtgaagca aaagaaaagg cacttacagc taaaaatcca 1140tttctgttca ctgtagctac attgactggg catgttattc gagcttataa aaattacaca 1200agcggttatg gataatggac cagcgcgtgt gaagaaggtg tcatatgaat tacagaattc 1260tggtgatcta atttctgata ttgcagagat ttctactatt cgtaaagaag actatgctat 1320taacaaggcc aaaagtgaat atgaagattt attgcaatgt aataatttgc caagtagtgc 1380tacaccacgt ggtcatcaaa tgccagttgc atttttagtt gtcgctagtg gtttaaataa 1440acatggtctc gattcaaaaa atcaacttcc ttatactcat gtggatgtgg ctggtagtgc 1500tggtgacata tacgtaacac caactgctgc tcctttaatg atgtttgcaa gacgatatgt 1560
attacctcgt ctaggcttca aatgacaaac ttatgaccca tttttattca atttttatta 1620cctttttctt ttataaccta tttgtctcct ttaacgtatc aacacgctct tttctatgca 1680cgtttccgta tattaattta caagaagctt aattaaaatt ccaaatacga ataaaatttt 1740atttt 1745<210>2<211>1760<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>2tttgtaaacc acaatacggc tgacatcaga gcccatagtt tcaaagagct ccacctcaaa 60cttaagagat ttcccatcca tgtacgcata ttcaggcgac gcaatcaatt cacaaacttc120tcccaacctc atagttgcaa caccgatatc ccaagcttta attacactac ctttcccaat180tgtaaattca aacttttcat ttcgggctct gcttgagtcg aatacttcac catgcttttc240accaccgaag ttagtaccga cataatgtac tatgaccgtg tcatcatcgc atggtttgat300gtccgagtag ccttctcgta ctactttctg gaaacattag tggaatgaac aaggacgcag360ttgacatgtc ggagacgcca aagcagtctg aagaggaata tctaaaagat ttcatagact420taagcccatc tggtgatcgt ggtattctca agaaagtagt acgagaaggc tactcggaca480tcaaaccatg cgatggtgac acggtcatag tacattatgt cggtactaac ttcggtggtg540aaaagcatgg tgaagtattc gactcaagca gagcccgaaa tgaaaagttt gaatttacaa600ttgggaaagg tagtgtaatt aaagcttggg atatcggtgt tgcaactatg aggttgggag660aagtttgtga attgatcgcg tcgcctgaat atgcgtacat ggatgggaaa tctcttaagt720ttgaggtgga gctttttgaa actatgggct ctgatgtcag cagaaacaaa gatgggagta780ttcgcaagtc aattattaaa aagggaagag atatccacaa tcctgtagct ggggctgaag840ccactattgt tttccgcaat cttttgaact tgacggagga aacagaagtc acatattgtg900ttggcgaccc tccatcaaac gtaccggatg agttggacca atctgttcgt cacatgaata960caggtgaagt cagccgtatt gtggtttaca aagacggtca ttcattaact tctggagatt 1020cggataaaga tagaatagtt tacgaactaa cactgaagtc ttttgaaaag accaaacacc 1080tctctggtat tacgtctttt ccagagcgaa tagcctatgc aaatacactt aaagagaagg 1140ccaacaattt cttaaaggat tctaaatttg attcagctat cgaattatat aaacgtttgg 1200acgatgagct gcagtacgta gtggctaatg ggcctacaga acaaaaggaa ttgtctgggg 1260tcactgtagc tgtccaactc aatttagctc tggtctatct gaagctttgt aaaccagaca 1320aatgtattga gttttgcaag aaagtgctcg ataattttag cgacaacgaa aaggcgctat 1380ttagaattgg tcaggcacat ttactacgta aggaccatga agaagcagtt gtttacttca 1440aaaggattgt aacaaaaaat cctaacaatg catctgctgt aaaacaggtt caaatctgtg 1500aggaagagat tagaagagca aaagatatag aacgaaagaa gtttcgtcat attttcgaac 1560gttgtaaaga cacgggatta aatgatgctc aaaatcatga ggatggggtt gtagtgaatg 1620atgaaaagtc tgccctgtga gagcttaatt ctgtgtgtgg catgaagtta gcaccccgtg 1680attttgtaaa tgtttcttat aattactggt ttaatttcaa agttgtgaat acattttaaa 1740gcaaacaaaa aaaaaaaaaa 1760<210>3<211>1447
<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>3tgacggcgtg attgttcatc gcgcatgact ggggtaaaga agaagcacaa agtcaaagat 60gcagatgttg gggcaatcca ggcggccgaa ccctttgcgg ttattccaga ggacaaaaag 120ccaactcttg actgcagtca gtggccgcta ttgttgaaaa atgtacattt aatgaatgtc 180cgtgaatgtc attttgaagc agacacgact ggcttctccc ctctgcatcg acctttggac 240gaatacataa agagtggagt tataaacctc gacaagccat gcaacccatc ctcccatgaa 300gtagttgcgt gggtgaagag agctctcggt gtcaataaga caggtcacag tggcacttta 360gaccctaaag tttctggttg tctcatcgtt tgcatcgacc gtgctactag acttgtaaaa 420tctcagcaaa gtgcaggaaa ggaatacgta tgcatctttc gactgcatga ttctgtagac 480gacgtcaaga aaatacaaaa agctgtagag ggtctaactg gcgcattatt tcagcgacct 540ccattagtcg cagctgttaa gcgtcaactc agagtgcgta cgatttatga gagtaaactg 600ttagagtatg ataaaagtag gaatctagga ttaataacgg tccgatgtga ggctggtaca 660tatgttcgga cgttatgtgt tcatttgggg ctacttcttg gttgtggagg cgttatggaa 720gagcttcggc ggtcgcgaac tggtttcatg tcagaaaggg acaaccttgt tacaatgcat 780gacatattag atgccaaatg gctgtatgac aatagacgag atgaaactta cttacgccgt 840gttgttatgc ctcttgaaag actgttggtc aaacataagc gcatcatgct caaggatacg 900gctgtgtgcg ccatttgctt tggtgcaaaa ctaatgttgc caggcgtact ccgctacgac 960aacgacatta atgtcaatga tgaaatagtc ttaatgacta ccaaaggtga agccgtagcc 1020cttgctattg cattaatgac tactgcgaca attgcgacgt gcgaccacgg attagtggcc 1080aaactaaaaa gagtaattat ggagcgcgac ctttatccta gacagtgggg tcgtggtccc 1140gtcgcaatgg agaggaagaa gcttgttgcg caaggattac tcaagaagtt tgcaaaatct 1200ggcgaaattg agagtactcc taactctgct ggacttagaa aagaagttga agctgtaaaa 1260tcttcggttg ccaaaacgct gaaaataaaa ccgaaccttt ctgaatctgg taacacaagt 1320gatgatgagt ttgaaaggca aaatccaaat gattatactg ggcagtacac ggagtctcaa 1380gaaacatccg atgacagcga ttagcttcgt ctttgtacag agcatataat aaactgtata 1440tttccgc 1447<210>4<211>1427<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>4tttttagtac tagattagac tatttgatgt tgttttcttc tttttaggat aataaactaa 60attgaatgaa tttagaatga taagtcatca agctggtcca agtataacgt atccagatcg 120tatcagcgct gaaagcgatg ctgaacaact gcataatgca tgtaaaggat tatcaactga 180tgaagaaaca attacaaaaa ttttagggca tagaagtcaa tatcaacgtc atcaaatacg 240tgaagctttt caaaggcgat atcgtaagga tttagttcat gtgttatgtg gtgcaacaaa 300aggagattat catagtctta tcaagacgtt atttcgtgga aaactacaaa tattagccta 360tgatttatat aaaggtatta agaaaccaga aattatcaat gaaataattt gttgttgtaa 420taacaaagaa attgagaaat taaagatagc atatgaagaa gttctgagag aagaagatcc 480
aaaaaaagct tcacaacgta cacttgagac ggatataatt aaagaaacaa aacctccata 540tgaacaatta ttagttgcat tattaaaagc taatcgcgat gaagattcac ctgaattagt 600tgatgaagcg attcgaacta gaaatacatc aagactagtt aatcgaattc aagtcgataa 660agatgttgaa gagttatatt atgctggtga aaaacgagca ggtaaaggtg atgcagatac 720attcataaga attttaacaa aacgaagtca atatcatgta aaagagataa tggatgtcta 780ctattcaaga tatgatcata cattagttga tgcagtgaaa aataaatttt cagaaccttt 840cagaactgga ttgaatacaa tgattatggc gttaaaagat ttacgtttat tgttggtctg 900tcaattatat gatagtatgt atggacttgg aacacgtgaa gacactctaa tacgaattgt 960ctgcttaaga tgtgaagttg atatgaacac attgaaatca atgtatcgtg aatattttgg 1020taaaccatta attgaagcag tcagagaaga tacatcaggt gattttcgta aattattatt 1080agcattaatg ggtgaataat aaacataaat ttaaaaaaaa aacgacgaga aaacagaaat 1140gataatcaaa agaaagaaaa aaaaataatg agaagtatat tgtaatgctt atcactatga 1200caacagagtt tctctttcat ttgaatcaat ttgttctagt tgttttatta taaacaattt 1260tattgttacc aattgaattc acttataaaa cataaaacat aatggatttt gtatatgcat 1320tagttaatta tgtttgtttt gcttatttaa atgatgatag caataatgaa tgtattacgc 1380ataaacataa caaatttgtt gatataatta aaatgatttt ggatctg 1427<210>5<211>1665<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>5cgcaaatgtg gaaatgttga ggtacacgaa ggttttacct ctgggtgttg gaaggaattc 60agtacttatt aaattctttt ctactgctag ctcaaccagt gctactcaaa caaccaaaac 120tacttacgga ccactctccg atgaagatcg aatattcacc aatttatacg gtcgacatga 180ttggcgtctc aaaggtgcca tgaaacgtgg tcactggtat aaaacaaaag aaattatctt 240gaagggtagt gattggatca ttgaagagat taaaaagtct ggacttcgtg gtcgtggtgg 300tgctggtttt ccatctggta tgaagtggag ttttatgaat aaaccaagtg atggcagacc 360taaatatcta gtagtcaatg cagacgaagg tgaacctggt acctgcaagg atagggaaat 420tatgcgtcat gatccacata cacttattga aggttgtctc atagctggaa aagcaatggg 480tgctaatgta gcttacattt atatacgtgg tgaattttat aatgaagcct caaatatgca 540actagctatc aaagaggctt atgaagctgg ctttctcggc aaaaatgctt gtggcactgg 600gttcaatttc gatgtctata tgcatcgtgg agctggagca tatatatgtg gtgaagaaac 660agctctaatc gaatccattg aagggaaaca gggtaaacct cgtttgaaac caccatttcc 720ggctgacatc ggattattcg gttgtccaac aacagttacc aatgttgaaa ctgttgctgt 780ttcgcctaca atttgtcgtc gtggtggtga ttggtttgca agttttggtc gggaaagaaa 840tcgtggcact aaattattca atatttcagg acatgtgaat aatccatgca ccgttgaaga 900agaaatgtcg attccaatgc gtgatttaat tgaacgacat gcaggtggtg tcattggtgg 960ttgggataat ttattagctg ttattcctgg tggtagttct actccattac tacccaaaga 1020tatttgtagt actgtcctaa tggattttga tgcactggta caagcgaatt ctggtttagg 1080tacagcggca ttaattgtaa tgaatcgatc ggcagatgtt gtaaattgta tttccagact 1140tattaaattc tatgaacatg aaagctgtgg ccaatgtact ccatgccgtg aaggatgtcg 1200ttggatgggt caaataatgt ctagatttgt taaaggtaat gctaataaaa gtgaaattga 1260
tatgatttgg gaaatttcta agcaaatgga aggacgtaca atttgtgctt tagccgatgg 1320agctgcatgg cctgttcagg gtcttattcg acattttcgt cctgaacttg aagctagatt 1380tgctaaacat ggcatagaac tacacgaacc tctcaaagct gaatcgagta ttccctgtta 1440atcttaacat taatgcttcc ttctgcatta gcttatgttt ataaaagttg attttatcaa 1500attaaactat tatcctagtt tcctttttgt atcatgtctg tagtttgatc agtggttagg 1560tatatcttag tgtgttttca agtttaactt cttcgaaaat tcctatttcc acattgtctt 1620taggaaatat atatttcatt gttctctaaa aaaaaaaaaa aaaaa 1665<210>6<211>1673<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>6taaacaaatc ctttatcata aaatgcggac atctgcgttt caaagtttga cttcataatt 60tcaaataaat cggtcattca gtagatggtt taaaaatacg taatgtattt acgatacaca120ttggaatatc acaaatatgt cactatatat atatatatat atatatatat atatatatat180atatataccg gttttaaagt tagctgccaa aacatataat atatttctac gtaactgact240ctccactgta gcactttatc acttaaaatg tcgatcaaat agagtttact atatgcttct300tagtacttcg tgtggacaaa acaagcaaca aatgaaaata gtaacttcac ctcgcttaaa360ataggttttt ataacgcaac atacatccta tcatttaatt gaatatactt actaaacaga420caaatacaaa tgaaagttat taatccaaaa taaagttaga agtaacaagc agtttgttaa480caaacatttt agttgtgcat tacattactt gtaaaagaaa tcgccacaac tacacaatta540ttatatttta attccccaga atttttacat ttgtcacagc ctagctttat ttatgatgac600tacgacggca ttatatataa tattcatcta tcaaacgcgt gttattgtct ccgttaaagg660aatttaccat gtatagaaag gctggtattg catcaattaa aagtaaatca cttgctcagt720cgaagtataa agaaaaaggt gatgagttag cggagaatca gataacaact atatcaagac780agtttgacca agttcgtcat tcgcttgaat tatttgctag caaacatggt aacaagatta840aagaggattc acagctacgt tcacaatttc aagcaatgtg tagcagcata ggcgttgacc900ctatagccta tagtcgcgga tgttggtcag aaacactagg tttaggagaa ttttattacc960acctgggtgt gaaaataatc gaagtatgca tggctcacga aaagcacact ggaggaatta 1020tgcctctacg tgagctggta tccttattga ataagaatag aacacaatat gagtctgaag 1080ttagcactga agacgtaaaa agatctatca ggaagttacg ttgtttaggg acaggattca 1140gtctcattag tttacctggt ggtcggttat tggttcagtc ggtcccgggt gagatgaata 1200tggatcagac cttagtgttg gggctcgcag aaagttctga cagtcatgta acagcaacag 1260ctgtaatgga gaaattcaat tggaccaaag accgtgcaga tgccgtattt cgtcatttaa 1320tacaagaagg aattgcatgg gtagataatt tagatccatg tggtgaaaga gtattttggt 1380ttccgagttt attggactca acgattacgt catgaatgta tatttcatta tactatttta 1440tttttaatcc caatattttt tcggaagatt ttttatcaat tagtttctct atgtaatgga 1500ctcacgaaag ataataacta actatcgcaa aagatttgac tttttaaaca atttatcatc 1560aagtccagag agttaaatac atctccatct ttaatgaata tgattttgag tagtagaaat 1620atgcactagt gatagtgtat tgttgtatta attatacagg taactttaca atc 1673<210>7
<211>877<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>7ctttcagttt gctgttcgtg ctaacatggc aaaggtgaag tgcattgtca gacgaaagcg 60ggtgtcagcc aagcgacagt accaccatct aaagttcata gctaatgtaa ataaaaaact 120aaagaaagca ggaaaagatc cattgactgg gttgaggact cctaaaacaa aagaagcttt 180agtgaagcct aaaacgcgtt tccctcgcag ctatgtaaca caacagggaa agcgaaaact 240acgcctgcgg agagcgaagt gtttctcaaa tcatgtccga aagatccgtt caagtattca 300gcctggtacc gtacttatac tgcttgccgg acctcataaa ggcaagcgcg ttatctttct 360aaaagcccta aagagtggtc tgctacttgt cacagggcct ttcaaatata atggtgtccc 420acttcggaga gtaaatcagt gttatgtcat agcaacctct actcgtatag acatatcaaa 480gttcgatgtg cctaagcata ttgatgacga gtactttcgg cgtgttgacc tgaaagaaag 540caaaactgac gctgggaaac tattcagtac tgagacaaag aagtatactg tcagcgatat 600tcggaaagaa gaccagaagt tggttgacaa tcaaattcgt gcagctataa aaagccaccc 660tgactcaaaa attctccacg cctaccttcg ttcactcttt tctttaggaa aaagagatta 720cccacataaa atgatctttt aacgttgaga taatacactt ttgaaagggg gaaaactacg 780cagggacgtg agccgatttc ccctagggca aaactccaaa cccggggggg ggccggtacc 840catcgcccta aagggatcga ataaattcca ctggcgc 877<210>8<211>1351<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>8aattcggtca tatttgagcc gggtagttaa acagtcatgg agatgacaga tcgggaaatt 60ctcctatttc gcataaaatt ggtagaacag gcggggttgt atgatgatat ggttagtgca 120gtgaaaacac ttgtagagag gggagaagag ttaaccgtgg aagaacgtaa tctgctttca 180gtagcctaca aaaatgttat tgggcctcgg agatcatcat ggcgcgtgtt tagtgcaatt 240gaacaggctg aaggacaacg aggtaacact gaaaagcaag caactgcaaa aaaattccgt 300gaagttttgg aaggcgaact ggattccatt tctagagaaa taatcgaact tattgataag 360tacttgattg acagcgcttc aaagccggac tcaaaagtct tttatcttaa gatgaaaggc 420gattactacc gctatatggc ggaattctca gtcgatccac aacggaaaaa agctgctgag 480gagagtaata aggcctacca ggaagcatct gaagttgctg cgactcagtt attcccagcc 540cacccaatca ggttgggatt agctctcaat tactctgttt atttctatga aattatgaat 600gatccagcta aggcatgtga ccttgcacaa acagcttttg atgatgcgat cgccaaacta 660gaccagttgt cacaagatat ttacaaagat tctactctaa ttatgcaatt attgcgagat 720aacctaactt tgtggatgtc agaatatgaa agggatgata acgcgaagaa aggtacagac 780gagaaggcct agtgactgga cttagatttc gctggtgctt gttaatctgg ccagagttgc 840ttttaggttt aactaaattt attaacacta agttttcaag agtcatgtag gtgcacacca 900gtagtgcatt ttatcgtttt accattttga tttttgtttt tccaaattat taccaagtta 960tcctagaatg ctggttctca tattttcatg attttgttta tctaggtagt cattatctat 1020
catcaattat attacatact ttgaaactta acattttatt aatctgtcct taatccgact 1080gatactttat gttgtttaca gttaaacctc atgtccacaa cttgagcatt tattattcag 1140ttctggctaa cgtgtaatga tttgattctt aacctcaaaa aagatttcac gcatatttta 1200tctcgatttg ataaactaac gtacacatga agtcattttg ctttgtattc ttttcttcat 1260gaaactgagt taactacttg gtatattaat ctctgaaata tatacacggt tcacgttttt 1320tgttaaaaaa aaaaaaaaaa aaaaaaaagg g 1351<210>9<211>1000<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>9cgcatttcgg attgtgtacg tgattgtgac ttcaggcacg tcagattgta tggctcccta60caaaattgta ttcattcgtc atggggagag tgtttacaat gaagaaaata gattctgtgg 120ttggcatgat gcagaccttt cagcgcaagg tgtcaccgaa gctgaacaag ctggtcaatt 180actccaccaa aatcagttca catttgacac tgcctataca agtgttctaa aaagagctat 240taaaacgtta aactttgttt tagacaaact cgatcttaac tggatacctg taacgaaaac 300gtggcgtcta aatgaaagaa tgtacggtgc tctccaggga cttaataagt ctgaaactgc 360tgctaaacat ggtgagcaac aagttaagat atggagacgt gcatatgata tacctcctcc 420tcctgttgat atttcggacc ctcgcttccc tggtaatgaa gctaagtatg ctttacttga 480ctcttcttgc ataccacgta ctgaatgctt aaaggacact gtacaacgtg tactaccatt 540ctggtttgat actatttctg ctgatattaa gagctgcaaa cgtgttctta ttgtcgctca 600cggtaacagt ttacgagcgc tcattaagta cttggataat acatctgatt cggatatagt 660ggagcttaat atacctactg gtattccact cgtttatgaa ctagatgcaa acctaaggcc 720aatcaaacac tattatcttg cggatgaagc aacagttgca gctgctatag atcgtgtagc 780gaatcaagga aagaagaaat aaagcaagac tgtttctatg gttacttttc acatcattta 840ttcattgatc attaatgcac tttcttccca ggaattttag ctatctgtag gtcttagcta 900tgaacaaatt gtctggttcg taatttgatt gtatgaaatc acgtaaatat atttttcgtt 960ctgttgttta aattttgtat cataaaagca cgtagtgact1000<210>10<211>1261<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>10tatgaaccaa ccaataatca caattcttgg ttaatctccg taaaacgtgc tcagctataa60ataacctgct ttgatctctc tgttaactac actacttgcc tatacttagt caaaatgtct 120aaagtcaagg ttggaattat tggcggttct ggatttgatg acccaaatct cttcaagcaa 180gtagggatta gaaaagtaac taccccattc ggagacccat ctgatgttct cactgaaggt 240tttgtcggtg atgttgcatg tgttgtactt cccagacatg gaaagggaca tttaatcctg 300ccaagtgaag ttaattatag agccaacatt tgggcgttaa aagaacttgg gtgtactcac 360atattggcca ccaacgcttg tggcagtctg caggaggaca aaaagcctgg cgattttgtt 420
gttcttaatc agttttacga caatactcga ggtcgagagc aaacgtttta tggaagtaga 480cctggttcat tagacggagt cttacatatg ccgatgggtg acccattttg tgaagaaacg 540cgtcagattt taatcgaagc agcaaaaaat ctgtcaatga ctgtttgtga caaaacgtgt 600agtgctaata caacttgcag tcatccatgt gtgcatagtg aaggctctgg aataactatt 660aatggtccac gtttttcaac tcgttttgaa tcattattat ttaaaagttg gggtttagat 720gtgatcaaca tgacactagt gcctgaagta tcactggctc gtgaagcagg tttgagttat 780gcttcgttag ctattgtcac agattatgat tgttggaaaa cagaccagaa acatgtgtgt 840gttgatattg ttttgaaaga atttcgtaat agtgtggaca aggttaaact aattttactc 900gaagctgtga aattgattgg tgcccgtgat tggactaaaa ctattgaagc gaacaagtca 960ttagtattaa gttcacgtca agatttagcc catcaagaaa gtaagggcaa ataaatgatg 1020atctaattcc aatcggttgt caatatattc aaccgcgtga aggaataaca ttttgggcat 1080ccttttacaa gtgttttcaa ttaatattta tttcctcttt tatatgagtt ggttcattca 1140attaacaata catgtagaac tcagtatatt tgtcatgtta aacgcagtat acttgtaact 1200atttattgtt atgtcttctg gtaacttttt acctaacaaa tagaacttag aaaattaaaa 1260g 1261<210>11<211>1820<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>11ccaaattttg aaagatcaaa tggatgacga atacgacatt gttgtacttg gcactggttt 60gaaggaatgt atactcagtg gtttaatgtc agttgagggg aagaaggttc tgcacatgga 120tagaaatgac tattatggtg cagaaactac ttcgataacg ccactcgaag cactcttcca 180aaagttccaa gtaaatgcag atctaaaagc atttggtcgg gggagagatt ggaatattga 240tcttattcca aagttcctaa tggctgatgg aaaattagta aaattgcttg ttcatactgg 300tgtcactagg tatctcgagt ttaaaagtat tgaggaaagc tacgtttacc ataataaagc 360agtacacaag gtcccgagta ctgcggcgga agctcttagg acaaccttgg tttctcattt 420ttcaaagaaa aaattaaaag atttcttgca gtgggtcgcg gacgttgatc ctgaaaatcc 480ttcgacctgg accggggtat atcccccacc gaaaagtata atgaaggatt ccattgaatt 540agcatttaaa cattatggtg tggaaagtgc tatggacttc gtcggtcatg ctatttgttt 600gtatactgac gattcttaca aacaaaaggc accagctatc gaagtcatca ccaaaatgca 660attatataac cgttcattga atcggttcgg atcatctcca tatctttatc ctctttatgg 720cttaggggaa ttatctcaat cttttgctcg acttagtgca gtatacggcg gcacatatat 780gctaaacaaa ccgatagaca aaatcgtggt cgaaaatggt aaagtagtcg gagtgatgtc 840tgagggtaaa gttgcacgtt gtggcaaagt gatatgtgat cccagctatg ccccagatcg 900cgttcagaag tgtggtcagg tcgttcgtgc tatttgcatc ctaaaccatg ccattccaaa 960tgtaaagaat tctcattcat tacagataat cattcctcac aaccaagtca atagatgtaa 1020tgatatatat atttcttgtg tgtctcatct gcatcatgta tgccctgaaa aattctatct 1080cgtcttagta gcaacaactg tagaaacatc aaatccacat caagagcttc aatctggtct 1140acagttactt gggcacattg aacacatatt ctattcgata gcagatttat ttgaacctgt 1200cgatgatggc aagtcaacta atatattcgt ttctaaaagt tatgacgcat caactcattt 1260cgaaagcaca tgtactgatg tgttaaatat ttacgagcga ataactggac atccgtttga 1320
ctttagtaag gtcacaagga atttggaaaa tgagtgatat agcaattgca ttgtcacatt 1380tcaaatgtcc tattaaataa atgggaaccc accatattat tttttcgcct tcttttcgtt 1440ttttttcttc agtaaattca cttataatta ttaccacgtt gtgtcattca ttccaactat 1500cactactaca atatccaccc tgtacttcta ttgtgatcaa cctgatacac gacagtgaca 1560ctttgtgttc attatctatt acttctaact actaattatc gtgacatctt aattgttatt 1620caaaattcat ttttgaaaac tggtttaatc aatcttgttc tgttgaaaat gcttaaattt 1680aaattgtttt tatttgttgt gatatcaacc cctccccttg ggaaatattt tattgtgttt 1740catactcact ttttgtacta tcctgtcata atatccattc cttgtcgtat tgaaacaatc 1800agtctctact agttgcaaaa 1820<210>12<211>1989<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>12ggcgcatcaa ttgcatactt ctcttcagct aatatttacc tctccatata tggttatgga60cacaacatgt tcacctatga ctgaaaggta cttgttaagc acctatcggc atgtgctgga 120actctttatt ctcatttcaa tgtctgtatt tggtgcgaag tctgtgttga agctatttaa 180gaaatataaa ggagagtttc ttcgcgccgg attcgcaggt gttgatatga ataaacctac 240taaaccaaca atccctgagg cccagggagt tatatgtggt gttgtgtttc tgtccatcat 300gtttcttttc atccctgttc ctttttggag attccttgtt agaaaagctg aaacacagtc 360aaatgaaatt tgttcatctg ataaaataag ggaaggacag gaagctttct ttaaaagtca 420gttcattcag tatcttgctg gcttactctc tgtatgctgc atgttgtttt tgggatttgc 480cgatgatgcc ctcaacctac cttggagaca taaaattggc tttccatttg ttgcaggctt 540acccttattg atggtttatt tagctaacga gggtaccaca cgtatagctg tcccagttat 600gttcagaggt tctcttggtg gtagcgtaga cataggtgtt ttgtattaca tttatatggg 660tttgctgact gtgttctgta ctaactctat caacatatat gctggaataa acggtcttga 720agttggccag gcaattgtaa ttggtgcttc tttgatctta tttaatctga ttgaacttat 780gggttatcac tggcgtgttc accttttttc actctatttt ctgattcctt ttttggctgt 840gtgttggtcc ttgtatcgtg taaaccgata tccggcgaag gttttcgtgg gtgatacatt 900ctgttatttc gcgggaatga ctttcgctgt tgttggtatt ttgggtcatt tcagcaaaac 960actgttgtta ttctttttac ctcaaattat caactttcta tatagcactc cccagttgtt 1020tggcttgatt ccttgtccac gacatcgatt gccgcgatat gattctacag atggtttatt 1080acaccccagt cgagtccgtt ttcatccaaa atctttatca cgtgctggtc agcttgtttt 1140gagtgttttt tctcacttgg gtataattga atgcaaactt tgtccagatt gcatcccgaa 1200tattcctgca gcactcaaga cagaaaatgt tgctcctcaa gcaggccatg aaaatatgtc 1260ttctgatgaa gaacaaatat ctgaaaggca aagagctaga agtagaagcc cagttcatgc 1320gaagctaccc actgaagaaa ttgttgaagt agacaatctt actgtcataa atatgatgct 1380acgtgtcttg ggacctaaaa atgaacaaca gaccactaat actctcttgt tattgcaagt 1440attttgctcg tgtcttgcat ttatgattcg ttatccgctt gcatggttct tttttgatgt 1500gccagccaat taaatgatga agatattttt ttgtgaacat tgaatgactt tatttaacct 1560tttagttgta tttaaactca ttattagagt aacctaatac ctgtgtccat tatttacgcg 1620acgaacggtt gtatattaat tattcagaaa tatatttttc ctttttgtgt agatttactt 1680
attaatgtgt ttattccata cttattttgg aggagtttcg ttgtgtaagt cagtgatata 1740ttagctgttc ttttctctgg tcacggaatc tactactgat gtatgtatac agacacacaa 1800cacaacggca tacatccaat ccattttctt ttacattttc gatatgtttt gattgatgtc 1860gtctattata atgaattctt acttcattac attatttgtg taatcgtaca atgaatttgc 1920taagaaattt tatcgtcatt aaaaaataaa cttgttaaag ctcacaaaaa agaacaacaa 1980gacaaaaaa 1989<210>13<211>1373<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>13atcaatcaaa agtttaacgt gttcttgcta gaatgaaaaa atttatggac tccactaata60caaagtttaa tagagctata cagaaaacaa gcgaaatcat cggtagagct gaaaagacag 120aatttgatga tgatttcgtc gcactcttaa aagatgcgga atctactcat gaggcatcca 180aacatattat ggaggcaatc gagctgtgga taaatccatg tgttttgagg aaatttgatg 240atgttgcttg caaagtagaa agtatggtta ctgacaacaa aagttattgg ttaaaggtac 300cagcaaaact acctgcggag catcttggag atacattagt caacgcagct atggatgtgg 360gggttaacac tcagtatgga tcagcactga aacattgcgg agagtatggt cgtgagatag 420cagcgattga aagtcgaagg aattcagtac tagaaaagaa tacattatat ccacttcatc 480gttttttggc aattgaatgg cctgatatac aaaaagaacg tagtcatttg gaatcagttc 540gtctggacta tgatcaatta agaagtaaag tgaagcacaa tgattcttca gatcctgaaa 600ctgggaagaa gttggaaaat gctaaagata ttctttataa acaactggaa gtgactagat 660ctaaactcca actcgttaag tcagtaaatc aaactaacct agttgctata aaagaattgg 720ttgctgctca acgaaattat ttcaatgaat gcaaacaaaa gactgaagaa ctggcttctc 780atttggaagg attacgttaa atattttttt tttggaaggg taaaagaatc tattcaagaa 840cttccactaa taacgtcagt tttgtgtttt ctttcctaat atatgcaagt atacagcctt 900actctttata gtgatcatgc tattgaatta ttttgttgaa tctggaataa tgatctgaat 960aataattatt gcagtgatat atctgactat tttgttctta atttacagat aatactcatt 1020tcatgcttaa aatacttctt ttttttaatc gttatctcaa tatacttttg ctcttcaatg 1080taatttccta tttggtattg attatctgcc ttataaatgt taatactact cgttgtaact 1140acatactact aaatagaaca acaagtatat tttgtcctct agctatttac aagcggtgta 1200tgtgttcgta tctcttctct tggctattgt atattttcat aaatatattg ttactatggt 1260tgggtgaata gagtgtaatg atgatacatt tcattatttt atagttgaac tgtggcatat 1320aaatatttca tactgtttac aactcatcaa atacacgtgc ctgccaaatc ttc 1373<210>14<211>1351<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>14cgaatatgtg tttgaaagta gcaccaaaag ttcaagatcc atatgctaat aatccaagtc60
ataaagcatt tactcgtgcg tttctttcac aatcaactgt agattttgga gaagataact120ttttagcatc accacttggt gtactcttta cacttggtat actattaggc tctggtggtg180ctcaaggcaa aacaagttat caaatagcta aaacaatgag attgaaatca gcaaattttc240catggaatat ttcagaaact caacaagaaa tgaaatcttt atataaagag cttagtgatt300cacttactat ggagaataca acattagatg ggaatgaagt taaagttgta cgaatatcaa360ctggtgtatt tacacaagag acatacgatg tagaatcaaa ctttaatcaa agtattaaaa420atgattttgg aggtgaactt ttaacggtgg attttactaa tcaaacaaat gctgcacagg480atattaacaa atgggttaat gaacattcga atggtttagt ggaggaattt tttactgatg540atattccaaa agatgcctgg atgatactga taaatatttt ctattttagg gattattggg600agaatccatt tgtaccgtat tatacaagtt tagaaaattt cagtgttaca aataatcatc660atcttcaagt tcctatgatg tcaaaagatg aaatattaaa gtataaaaaa tttcaagaag720atggatttga aattgtcagt aaaccaatga agaatacacg tttttcattt attatcttat780taccattgga gaaatggaat ttaaatggtg ctattgaagt attaaatggc aataaaatat840taagtcatta tgttgataaa ttagaagaaa ctagtgtatc attaaaaatg ccaaaattta900cattaacaaa aaaacttcaa cttgtcggaa cattaaaatc tattggtata aaaaatttat960ttgatccatt aaaagctgat ctatcaggaa tctcatctaa aaacaatctc tatgttcgtt 1020cattcattca aactaatgta atgaaagtga atgaaagtgg aattgaagca gcatctgtca 1080cttcacctat catagtacct atttcagctc ttataccaga tgtcaatttt catgtcactc 1140atccatttat atgctttata tatgatcaac aattaaatat gccattatta gcagctaaag 1200taatcaatcc aatgttataa tctaatgtga ataaaatatc tgattgatga agtttcatgt 1260cataactatt gtcattttga tttattagct aatagtggtg aatgaatgca gaacttattt 1320gattaatgac aatataaagt acatacaatt g 1351<210>15<211>2132<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>15aaaatcatga aggctacaca aaagaaataa aagtatccta cacaattttc atactttaca 60agttgtctat agtttttgcc aagaaactat tcattcattc attcattcat tcattcattg120aaaagaatat tcttatacaa ttgaatttag acaaaatatc tattacatat taaatactac180tactaattta ataaagtaaa ttattattca agtgaaataa aatggctaca cgaatgagtg240atatgggaat ggatgtacag attaatcgtc ggttgaatag taaatatgat gcagatagtg300aagctgaagc tattggttgg ttaaatcaac tgactaatga aaatgtacca tttggtagag360aaaatgttgc agcagctttg aaaaatggac aaatattaat caaattgatc aatgctgtat420ttgatgggac tccaactctt ccatcgtctg cagctaaaat gaaaagaccg ttaaaagcta480atacaatgac tgctccattc aaacagatgg aaaatattca aatgtttctt aatgcagcag540ttgcttatgg agtgcctaga gctagtctat ttcaaactgt cgatttgtat gaattacgta600atatgccaca agttttgaac acaatattac aacttggaac agagtgtcag cgaaacaact660ttaatggacc agtatgcggt ccgaaaccaa cctatgaaaa taaacgtgag ttcacagaag720aacaacttag ggcttctgaa ggtataattg gtttgcaagc tggaactaat aaatgtgcca780gccaagctgg aatgagtcat ggtggaccac gacacatcac agatatcaaa gtggatgcag840cgtctaaaga aagccaaggt gttattggcc ttcaaatggg atcaaacaaa ggtgctagtc900
aagctggaat gagtcatggt ggaccacgac acatcacaga tattaaagta gatgcagcat 960ctaaagaaag ccaaagtgtt attggtcttc aaatgggatc aaacaaaggt gccagtcaag 1020ctggaatgag tcatggtgga ccacgacaca ttacagatat taaagtagat gcagcatcaa 1080aagatggtca gtgtgttatt ggtcttcaaa tgggttcgaa taaaggtgct agccaggctg 1140gtatgagtca tggtggtcag agacatatcg ctgacattaa agttggcgac atgtcaagag 1200aaggctcaaa tgtcattggc ctacaaatgg gtacaggtaa agatcaagta gcttcacaat 1260ctggcatgag ctttggagca caacgtcata ttgtcgatac acattaaatg atgattatta 1320ttgcctactt taaagtttat cttattaggc ttcttgctta tttgcaattg catcatcaga 1380tttatcattc ctgtcttgct ttattattat tttcatcaat ctgcttatca ttgaaccatt 1440tcacatgttc tctacaatta atcatgttta tacttaaaat gatcataagt aggaagcaat 1500cactgtgaga agcatcaatt gcttattatc ttcaatgtat cttatattca atcttttatt 1560ttatttacta agaatattca caaactttca agtgatgcat catgattgtt tctttgttgt 1620catagacaca attcaagatg atattcaatc ataattcaac atagtaagta agaatttgtg 1680attttctcgc aatgtttctc atgtacactg aaatcagtag tatgaatgtt aaatgaaaaa 1740aagaaaacaa agaaacattt tttttttcat tctttaatta tgcaattttt gtgaagtcta 1800tttatttcaa gctaattaaa gcttctctta ttttttagta acaagacaaa attgttgcca 1860tgggaatgtt cacagttttg ttgacaattt ttatttacaa ttttatgttt tttttttcag 1920gctttcgatt tgttttttta atcataaaaa ttaccggaat ggggttgttt tttctaaaca 1980aaaaattgtg tatttatttt gcatgaatct gttttatcat cgcttcgcta ctactactac 2040tactactact actactaata ctactattga taataataat aataataatg ttaataaaaa 2100tttgtaatac aaaaaaaaaa aaaaaaaaaa aa2132<210>16<211>1824<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>16ggtacccggg aattcggcca tgcaagcgcc gcacaggttg ttaataatgt tggctttacg60tcttcaagtt cgaagatctc tgactaggaa tatttcagga tggtcagata atttgcgtaa 120gcaatcgaaa ctttctgaag ttgtttattc acgcgaagat aaatatggag cacacaatta 180ccaccctcta ccagtagcat tatcaaaagg caaaggaatt tatgtgtggg atgttgaggg 240aaactgttat atggatttct tgagcgctta tagtgctgtt aaccaagggc actgtcatcc 300acgtattgtt gatgcaatta aaacccaatc ggaaacacta acccttactt cacgagcatt 360ctataatgtt gttttgggag aatttgagga aatggctaca aaaatgtttg gttatgacaa 420ggtgttaccc atgaattcag gtgttgaagc tggtgaaacc tctctaaaac ttgcacgtaa 480atggggttat aaggtaaaga aaattcctga tggacaagcc agagttatat ttgctgaagg 540aaatttctgg ggtcgtacgt tagctgcatg ctcttcatcc actgatcctg catgctactc 600aggatttgga ccatttatgc caggctttgt aattgtccct tatgatgact taaatgctct 660tgaagttgaa ttaaaaaatc caaatacgtg cgcatttatg gtggaaccaa ttcaaggtga 720agctggcgtt cgtacaccat cagatggtta tttaaagggt gtacgaaaat tgtgtactaa 780atataatgta ctatttattg cagatgaaat acaaactggt ttaggcagaa caggaaaacg 840actttgtgta aatcatgaag atgttcgacc agacattgtt ttacttggca aagctttatc 900aggaggaatg cttccaatct ctgctgttct tgccgatgat gaagtaatgc tgactattca 960
acctggggaa catgggtcaa cttatggtgg taatccatta gcatgtagag ttgctatggc 1020cgctttacgt gttttagaag aggaaggttt agcagaacgt gcacagaaat tgggtgaaat 1080cttccgcaat gaattgagta aactcccaaa atctgtagtt gaattatatc gtggcaaagg 1140gcttttaaac gctattgtaa ttcgtcaagg attcaatgca tgggatgttt gtttaaagtt 1200acgcgatcat ggtttattgg ctaagcctac acacgacaca ataattcgat tagccccacc 1260tttagttatt aaagaagatg agttgcataa agcggcagag attatgcaca aagtgattgg 1320ttcgttgagt aattagagaa ttgaaagaag aaaacaatag tagccctgat attttgttaa 1380atggcctgtg tttgcttgtt gctctaaatt tcatttgatc gaatcggcta caaagtgatc 1440tgctattctt taatgcatca atatcatcga tgtcgtaaat cagtttaatg gattaaagaa 1500acgccttttg ttcatacttt cataaattat tcacttgatt tatttatttt tatttaaatt 1560aatcattata atggtacttc acaatttgct gtttgttgct tgacaattac cgtttgtcta 1620ctgtgattat ttccaagtga acttgtttca tttgtgctat tttttggtat aaaaaactta 1680tcttgatacc tgattttgaa ttactcggtg cacttcatat taatttttta cttatgatta 1740tgtgtgctta tatgaatgtg tgttcagaga taatcagaga aacgctaagg aacaaatcgc 1800tgactgaacg ctatttacga aagc 1824<210>17<211>1680<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>17ctttgcttag ttttttatta agaacaaatc attttaaact tacaatcaca ttagtcaatt60aaaataatta ttattcattc aacattgtgt taataattgc agtttatgtt actgatcaat 120gttactcatg aatcaacgtg ataacattga ttcgtggaca aaatgcgaat tcatttgcgt 180ccaaaagttg aataatcgat atctccagcg ccccaagcat tgactcctag actgcaatat 240tcagagaata ttgctaacac tgccaccatt tgaacaacac agagaaacat tcggcgagat 300ataagctaca tcatgtgacc aattatcact ctgactttat ttgacataga tacacatatt 360aaatacataa atacaaacag aattttaata atctaatcat ttattcagtg atatattcag 420ttaatcaaca ttcatttgaa ctcatcaaca tatttactga tatatatcaa tagatctatt 480caatatttac attttaacat tagtaactat gacaacaata gagtcattga ctcaagaaca 540atgggatgat ctatttcata attttgaatc attacgtgaa tcaaataata ttgcatggag 600tgatctaaaa cgtgcattag aaatagttgg tgtatctgta gctggacatg aaataagaga 660tttagttgga caaccacgta attggcttaa tttagctgaa ttcaatgatt tatatatgag 720agctaaagat atgaaagata caacaaaagc aatacgtaaa gcattactat tgaaacattc 780tgaagatgtt aagtcgttta ctgttggcaa aaatgatact gatacacgac attcagttag 840taaagctgaa gaaagaggct ttacactatg gattaataaa cgtttaggtc atgatacaga 900attacaaaat gaaatattac caattgatcc atcaattgat ggtcaattat atcaacgatg 960caaaaatgga atattattat gtaaacttgt caatgttgct agtccagata ctattgatga 1020acgaagtata aatcgaggag ctgctttaaa aaatgttttc aatgttcatg aaaatttgac 1080attagctgtg aattcagctg catcaattgg atgttgtgtt gtgaatactg gtccagaaga 1140tataatgcaa ggcaaacgac atattgtttt aggattaata tggcaattaa ttcgtcgtgg 1200tctagttgat acaattacat tgaataaaca tggtgaatta ttagctttat tacatgatgg 1260tgaaaatgct gaagatttag ctgcaatgaa accagaagaa ttattaatgc gttgggttaa 1320
ttatcattta catcgtgctg gttgtgatcg acgtataact aatttcaatt cagatttagc 1380tgattcagtt gtatatgcac atttaatgga acaaattgta ccattagata aacgttataa 1440tttaatgtca gctgatgaaa tacttagttc aactagtcgt caagaaagag ctattaatgt 1500attaaataat gctgaaacat tgaatacacc atttttatta gcaccagaag atatttatct 1560tgctggtgat aaagataaag aaaatcgtga tcgattaaat ttagcatttc ttgctacatt 1620attcaatatg tatccaggtt tagatacaac acgtggtgat ttattaatag aaggtgaaac 1680<210>18<211>1653<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>18tgcatacgtt gatagttatt atccgttgac agtttttaga tttatggtgc atcaaaaccg60tattttacag ctcttcctgt ttatttgcac gtcatttgtt tcgtcatcat ccataccatg 120gccaccaaag gaaaactatt ttgatcagac tttggatcat tttagttttc aagcaagaaa 180cttaactttt aaacagcgct atctttatga agataaatgg tttaagccaa atggacccat 240cttcttttat tgtggaaatg aaggggaaat tggtgggttt tggaacaaca ctggattggt 300attcgagtta gcacctagtt ttaatgcttt tattctgttc gctgaacata gatactatgg 360gaaaagtctt ccctttgata aaagcttcca acagccatat attcagtacc tttcaattgg 420tcaagcacta gcggactatg cttatttaat tgagggtatc aaaagtaaat ttaacatgac 480tagatctcca gttgttgcat ttggtggaag ctatgggggc atgcttgcag catatatgag 540agctaaatac ccccatatag ttaaaggtgc tttagcagca agtgcaccag taaggtgggt 600tgcaggtgag ggaaattttc atgatttttt cgaagctgtt actaaggact atcatgatgc 660agatccaaaa tgttctgaaa aaatcaaaaa tgcctttact gttgctgtcc agttatctca 720aaaacctgat gttggttata aacaactctc tgagcaacta cggttgtgtc aacctatcca 780gaatgatttt gaattttact ggatgctgaa atgggctcgc aatgcatttg ttatgatggc 840tatgcttgat tatccgtata aagcatcatt tatggcaagt ctacctccta atcctgtaaa 900tgtctcgtgc aagaacgctt tatcagcgat tgaccccatt ccaactcttc gtgaggctgt 960tggagttttt tacaacagtt cacaatctct catgtgtttc gattacaaaa cacagtttat 1020tgaatgtgct gatataactg gctgtggtct aggcaatgat tcacttgctt gggattttca 1080aagttgtaca gagatgaatt tacacgatga ttcggatagc actacaagtg atatgttcac 1140atctttacct ttaacaaagc aacaagtaac aagttactgt caacaaaaat ggggtgttac 1200acctgctttt aatcaattgt caacattttt tggtgattat atttggaaaa ctgcaagtaa 1260tataatattt tcgaatggga atttagatcc atggatgggt ggtggaattt taaccgatca 1320atcagagaag gttatctcgt tgatgttaga tggtggagct caccatcttg acctacgatc 1380cccagatccc aatgatcctc catccgcacg tcaaattcgt caaattgaag tacaaacaat 1440tcgttcgtgg cttgattcgt aaacctcaga aaattgtttt ctttcctcct atccagacaa 1500atcatatctt tcatactgtt cactaccttt cttttgttta ggattatgat tatgatcacc 1560atttacttga tttgtgtcta gtactccaga tttctccata aattatttta ttattgtgaa 1620aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aac 1653<210>19<211>1716
<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>19cgagcacata ggattcgtgt ggtttaaaat aacttattat gtagatattg gatattgaaa 60cttaaagcac tactttcaac aataatgtta cgcactcaac ggggatcgtt ataagtaatc120tcatttctta gtacatcata taaattattt ccgtttctca tgttggcact atttttgtct180ataatgccat ccatgtaaat aacttgagca aaacccccag ttttttctgc aacataactt240gcatttagtt tataaacgcg tttaaccaaa actatgaagc ccagctgtaa aaattgctga300agtattttgt gtaacacatg aagtcaaaca ggcccgaaaa aggggagcat gtataagacg360agctattgaa acttatattc gccactctag ttgttttcta gcccctcgtt tggttttctc420ttacatttgc ttctcattgc ggacggattt cgccaacctt ctccctttgt atcagacaag480ttgctcactt tatcctcctc actacagatg atctttctat caacatctta cctacaaatc540tatatagtgg caacaatact tgttttattt gacgtcaaat acacgcgtat aactcacaag600taaatggcaa ggtgcaactt attgaaagga ataaaacgaa attctatgac aaatataaag660tctaagatga atatactgca gttagttaaa cagatggatg ctctctcacc tagaatccaa720gctttactaa aagaaagtaa tcgtttcaaa tctaatgaag gcaaaaataa tgaatctcaa780ttttcccggg agtcattaaa ggctagactt agtgacctgg aatttcgtgt gacacaaaat840aaagaaacag aaaaagcttt ttcaggaaag tacgtaaatg aaaagtcacc cggaatatac900aaatgcgttg tctgtagctc aaaactgttt agcagtgaag caaaattttt ctgcagttgt960ggttggcctg catttaacag ccctatagaa tataactgtt tagtagcatc cattgattta 1020tcatcaggtg atgtccgtgt agaagtttcc tgcaagcgat gcaatgctca tttgggacat 1080gtgtttgacg atggaccaga ccctacgggt cttcgttatt gcattaattc ttgtgcttta 1140gttctcgatc ctaaagttgg tagttcattg gatttaaaca tgccaacaac ttgatatttt 1200gttattgtca gataaatatt atgataatcg ttgtacgtaa actagaagta ttctagtgtt 1260gcatttctaa tctcaagctt cttcccccaa agtcttacaa tagaacttta taattttttt 1320tcactcttat tttactttta taatttgtat ttactgtttt tatttgaatt tacacagttg 1380ttgtgtagtt tatgaagtaa ctgtttatct tttactctgg taataaaact taactgttaa 1440gtatatgtgc gaaagggcat ttctccccaa tatgataggt gttttagtct taatgataac 1500ctaaatcctt tgtatgcggc gttttgtcga taatgtataa ctaaatacgt tcccatcata 1560tatgcaagca tttttgaatc atgaaattat cagtatttat caggttacta caagagatta 1620ttaagtctgt cttcgagatt aatcgatttg tatgtgatgt taatttttct cagtatctat 1680gagttctgtt tcaaaaaaac caacaaaaaa agtcgg 1716<210>20<211>2769<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>20acgaggatcc gcgttaacat tgaatagaat taaacaatca gtggaaacca atcaaattcg 60tggcctctaa ttaaattggt caacaaaatt tttacagtgt gttaattact gtaaacgtca120aaaccaatag tatcatttgt taaatccttg aaaatttatt gtgcgaatat gacgatgaaa180aacgaaattg tcgttccccc tggtctaagg gaactattac aagagttgac tgtgtctata240
ctacgagaac gccctgataa tttaattcaa tttgctattg atttcttaat gatgaagaaa 300gcagcatcag aaaatcgtcc aaacaaagca aatgaaagtg atgaagatga agatgaggaa 360cctatgccaa tgcctccaca aagggtttct cgaagagctg gtgtagctgc tgaaagctat 420gatccagaaa aagatgattc ttcaagtgca gtgaaagttg tacatcctaa gacagaagaa 480caacgtcaac gattaactca agcaactaaa gatatcttac tgtttcgttg tcttgatgat 540gatcagatga aggatgttat tgatgcaatg tttgaacgtc atgtatctcc tggtgaaaaa 600gtgattacgc ttggtgaaga tggtgataat ttttatgtaa tagaaaaagg tgtttatgat 660atcattgtaa aagttggtga tgaagagaaa gttgtcggaa agtatgataa taaaggatcg 720tttggtgaac tggctcttat gtataataca ccacgcgctg caactatatt agctaaaact 780gaaggtgtac tgtgggcaat gacacgtgaa gtatttcgtt caattgtact caaaaaagct 840tttgagaaac gtcgtctata cgaagaacta cttaatcaag taccaattct tgaaagtctt 900agtgcttatg agcgtatgag tattgccgat gcattaagaa cgaaaatttt tgaagccaat 960gagcaaataa ttaaacaagg tgatccagga gacgaaatgt tctttgttga agaaggtaag1020gtaagcatta aaatgaaaag aagcggtgaa actgaagaaa aagaagtagc tgttattgaa1080aaaggtggtt atttcggtga attagcttta ttgacaagtc atccacgtgc tgcttcagca1140tatgctgatt gtcgtacgaa acttgctgta ttggatgttg gaagttttga acgcctactt1200ggtccatgct tggatatact tagaaggaat atagatgatt atgaagcaaa attgaaagat1260attttcggca gtttagataa agtacctgaa ttacgacgtt aattatggat ttgtttattc1320ttattgatga acactatatg atcatctatt attgttatta ttattaatgt tatcacatta1380ctattcattg tattcaatat aatgaatcca tttttcaaat gaatatcagt actaattgat1440caattaatcg aatacttaaa acataaatgt cacaagtaac cggatagata atagtgagta1500agtaacgtac atattttgtt ttcatttgga taaataaaaa aaaaaaagac aaatttatgt1560acatatttgt atttttctca tccttattct attttctgat tatctacaat taaacacctc1620tctcatgcca tttcttttat tgtttcgaat gttttcatca gtaactcgta gttttgatta1680ttattattat cgttattatt actgttatta ttagcatcat tattatacgt gttattagtt1740cattttaatc tttgtcaaca ttttcattac cagtatcttg cacaaatgat tataattata1800ttcaactagt gattcaattc attcataaaa cattcataag aatatctact tgtctatcct1860ttctttcttt agttacatct ttttgtaagt tgttacagta acagttaata ttcataattc1920attcattcat tcattcattc attcttatgt agtttattgt gatgaacaat gaaagatgag1980aatttacaac tgaatgcaac caattattgt aaggtgaaag tgaaatgcaa ttttataata2040ttctattaca aattatttac ttttcctact ttcaattagc tttcatttcc tctatctcat2100gtactataat aatgtgcaaa tgaaaataat gattcactat agttagttac atgaaatcga2160actggtatct tataaactgt actgatatca ccatttatct tagttaaaat tatatttatt2220cgatcattca gtggacgtgg aacaatatat atatatatat atatatatat atatatatat2280atatatacat ttatatatac atttatatca gtaagacaaa tccaaagata cattaatgct2340ttatcaacaa acgttaacat tgttaacgtc atcattgaga ttgttctgat catgctgtgt2400gtatgctttt gtgcattggg tgatggaaat catctgtgta ttccttttgc taattctttc2460ttttctgtat tctctctgta tgtgtttgtg atttttgtta ttcttttatt cttcttcagt2520gttctttttc attttattca gttaattctt tacaggaaat caataaaaaa aaaggaagaa2580aaagtcttca cctatctgtt tcatgtatac atgcacatat tgattatctg ggtaataagg2640ttttaaaata tacttgaata acctgtcata tttattgatt aattttttgt gagttaaaag2700caatttaatg ttagttactc tattcagtag aaatttatgt tattaattgt tagtagccta2760cagtttcag2769
<210>21<211>655<212>DNA<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>21aatagggtgg taatacccag gaatgtctag tcctgtaatt ggcgctgtaa aatcaaaaat 60gcaagggatg aaacttcaga ttgatcagct taaacaagag gtatctagta aacaagcggt120gttaagaaag gaggaggaaa ataagaccaa ggctgaagct gaagtcgcct cactgcagaa180acggatccgg caacttgaag atgaattaga atctaccgag actcgccttc aagaagcaac240ccttaaactt gaagaggcca gtaaggccgc tgatgaaagt gatcgtgctc gtcgggtgct300tgaggctcgt cagactgcag aagacgagcg tattctacaa ctggaatcta tggtacagga360aactgcaaaa tctgtgaaag atgcagaaac aaaatatgag gaggctacac gaaaacttgc420cgttgctgaa gttgcactat cacatgctga agatcgaatc gaagctgctg aaagtcgttt480aaaggaactt caatcaatta ttcatggtac aatgggtcag ttaaaatcgt tagaacatca540agaatctcag ttaagtaaac aacgtagtct acatcaatct caattggctt ctttgtcaaa600acaattaatt gaggctgaac gacgtgtaaa agaagccagt catcaagagg aaatg 655<210>22<211>398<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>22MSLVTPAFPV FNLVDSAYDA VLLLNDDVEH LPNALQLALN ALKEFSEVNP KFSDEVSIIP 60FPQHPSKRLI FSPTGQLNTD EADIRNVYDA AVNGMLKLLK IGCKSPLLCC GSFVSAPKDA120QWSERNCLLL NAVLGAYHAL YTPLEVREML PEKFPKAIRF GVMEANDSLL NIAKAIEEGR180TLARDIGGSD PERMAAPKIA EYLKNALDGL TGVTITINKV DAKKYPLMAA VNRAASVVPR240HDGRVVHIDY KPSSSKEIDT SLFLVGKGIT FDTGGADVKA GGIMAGMHRD KCGAAAIAGL300MKTIGLLQPT GLSVSAALAF VRNSVGEESY VADEIIIARS GQRVRIGNTD AEGRTVMADL360LCEAKEKALT AKNPFLFTVA TLTGHVIRAY KNYTSGYG398<210>23<211>431<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>23MNKDAVDMSE TPKQSEEEYL KDFIDLSPSG DRGILKKVVR EGYSDIKPCD GDTVIVHYVG 60TNFGGEKHGE VFDSSRARNE KFEFTIGKGS VIKAWDIGVA TMRLGEVCEL IASPEYAYMD120GKSLKFEVEL FETMGSDVSR NKDGSIRKSI IKKGRDIHNP VAGAEATIVF RNLLNLTEET180EVTYCVGDPP SNVPDELDQS VRHMNTGEVS RIVVYKDGHS LTSGDSDKDR IVYELTLKSF240EKTKHLSGIT SFPERIAYAN TLKEKANNFL KDSKFDSAIE LYKRLDDELQ YVVANGPTEQ300KELSGVTVAV QLNLALVYLK LCKPDKCIEF CKKVLDNFSD NEKALFRIGQ AHLLRKDHEE360
AVVYFKRIVT KNPNNASAVK QVQICEEEIR RAKDIERKKF RHIFERCKDT GLNDAQNHED420GVVVNDEKSA L 431<210>24<211>459<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>24MTGVKKKHKV KDADVGAIQA AEPFAVIPED KKPTLDCSQW PLLLKNVHLM NVRECHFEAD 60TTGFSPLHRP LDEYIKSGVI NLDKPCNPSS HEVVAWVKRA LGVNKTGHSG TLDPKVSGCL120IVCIDRATRL VKSQQSAGKE YVCIFRLHDS VDDVKKIQKA VEGLTGALFQ RPPLVAAVKR180QLRVRTIYES KLLEYDKSRN LGLITVRCEA GTYVRTLCVH LGLLLGCGGV MEELRRSRTG240FMSERDNLVT MHDILDAKWL YDNRRDETYL RRVVMPLERL LVKHKRIMLK DTAVCAICFG300AKLMLPGVLR YDNDINVNDE IVLMTTKGEA VALAIALMTT ATIATCDHGL VAKLKRVIME360RDLYPRQWGR GPVAMERKKL VAQGLLKKFA KSGEIESTPN SAGLRKEVEA VKSSVAKTLK420IKPNLSESGN TSDDEFERQN PNDYTGQYTE SQETSDDSD 459<210>25<211>340<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>25MISHQAGPSI TYPDRISAES DAEQLHNACK GLSTDEETIT KILGHRSQYQ RHQIREAFQR 60RYRKDLVHVL CGATKGDYHS LIKTLFRGKL QILAYDLYKG IKKPEI INEI ICCCNNKEIE 120KLKIAYEEVL REEDPKKASQ RTLETDIIKE TKPPYEQLLV ALLKANRDED SPELVDEAIR180TRNTSRLVNR IQVDKDVEEL YYAGEKRAGK GDADTFIRIL TKRSQYHVKE IMDVYYSRYD240HTLVDAVKNK FSEPFRTGLN TMIMALKDLR LLLVCQLYDS MYGLGTREDT LIRIVCLRCE300VDMNTLKSMY REYFGKPLIE AVREDTSGDF RKLLLALMGE 340<210>26<211>475<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>26MLRYTKVLPL GVGRNSVLIK FFSTASSTSA TQTTKTTYGP LSDEDRIFTN LYGRHDWRLK60GAMKRGHWYK TKEIILKGSD WIIEEIKKSG LRGRGGAGFP SGMKWSFMNK PSDGRPKYLV 120VNADEGEPGT CKDREIMRHD PHTLIEGCLI AGKAMGANVA YIYIRGEFYN EASNMQLAIK 180EAYEAGFLGK NACGTGFNFD VYMHRGAGAY ICGEETALIE SIEGKQGKPR LKPPFPADIG 240LFGCPTTVTN VETVAVSPTI CRRGGDWFAS FGRERNRGTK LFNISGHVNN PCTVEEEMSI 300PMRDLIERHA GGVIGGWDNL LAVIPGGSST PLLPKDICST VLMDFDALVQ ANSGLGTAAL 360IVMNRSADVV NCISRLIKFY EHESCGQCTP CREGCRWMGQ IMSRFVKGNA NKSEIDMIWE 420
ISKQMEGRTI CALADGAAWP VQGLIRHFRP ELEARFAKHG IELHEPLKAE SSIPC 475<210>27<211>248<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>27MYRKAGIASI KSKSLAQSKY KEKGDELAEN QITTISRQFD QVRHSLELFA SKHGNKIKED 60SQLRSQFQAM CSSIGVDPIA YSRGCWSETL GLGEFYYHLG VKIIEVCMAH EKHTGGIMPL120RELVSLLNKN RTQYESEVST EDVKRSIRKL RCLGTGFSLI SLPGGRLLVQ SVPGEMNMDQ180TLVLGLAESS DSHVTATAVM EKFNWTKDRA DAVFRHLIQE GIAWVDNLDP CGERVFWFPS240LLDSTITS 248<210>28<211>238<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>28MAKVKCIVRR KRVSAKRQYH HLKFIANVNK KLKKAGKDPL TGLRTPKTKE ALVKPKTRFP 60RSYVTQQGKR KLRLRRAKCF SNHVRKIRSS IQPGTVLILL AGPHKGKRVI FLKALKSGLL120LVTGPFKYNG VPLRRVNQCY VIATSTRIDI SKFDVPKHID DEYFRRVDLK ESKTDAGKLF180STETKKYTVS DIRKEDQKLV DNQIRAAIKS HPDSKILHAY LRSLFSLGKR DYPHKMIF 238<210>29<211>251<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>29MEMTDREILL FRIKLVEQAG LYDDMVSAVK TLVERGEELT VEERNLLSVA YKNVIGPRRS 60SWRVFSAIEQ AEGQRGNTEK QATAKKFREV LEGELDSISR EIIELIDKYL IDSASKPDSK120VFYLKMKGDY YRYMAEFSVD PQRKKAAEES NKAYQEASEV AATQLFPAHP IRLGLALNYS180VYFYEIMNDP AKACDLAQTA FDDAIAKLDQ LSQDIYKDST LIMQLLRDNL TLWMSEYERD240DNAKKGTDEK A 251<210>30<211>250<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>30MAPYKIVFIR HGESVYNEEN RFCGWHDADL SAQGVTEAEQ AGQLLHQNQF TFDTAYTSVL 60
KRAIKTLNFV LDKLDLNWIP VTKTWRLNER MYGALQGLNK SETAAKHGEQ QVKIWRRAYD120IPPPPVDISD PRFPGNEAKY ALLDSSCIPR TECLKDTVQR VLPFWFDTIS ADIKSCKRVL180IVAHGNSLRA LIKYLDNTSD SDIVELNIPT GIPLVYELDA NLRPIKHYYL ADEATVAAAI240DRVANQGKKK 250<210>31<211>299<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>31MSKVKVGIIG GSGFDDPNLF KQVGIRKVTT PFGDPSDVLT EGFVGDVACV VLPRHGKGHL 60ILPSEVNYRA NIWALKELGC THILATNACG SLQEDKKPGD FVVLNQFYDN TRGREQTFYG120SRPGSLDGVL HMPMGDPFCE ETRQILIEAA KNLSMTVCDK TCSANTTCSH PCVHSEGSGI180TINGPRFSTR FESLLFKSWG LDVINMTLVP EVSLAREAGL SYASLAIVTD YDCWKTDQKH240VCVDIVLKEF RNSVDKVKLI LLEAVKLIGA RDWTKTIEAN KSLVLSSRQD LAHQESKGK 299<210>32<211>445<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>32MDDEYDIVVL GTGLKECILS GLMSVEGKKV LHMDRNDYYG AETTSITPLE ALFQKFQVNA 60DLKAFGRGRD WNIDLIPKFL MADGKLVKLL VHTGVTRYLE FKSIEESYVY HNKAVHKVPS120TAAEALRTTL VSHFSKKKLK DFLQWVADVD PENPSTWTGV YPPPKSIMKD SIELAFKHYG180VESAMDFVGH AICLYTDDSY KQKAPAIEVI TKMQLYNRSL NRFGSSPYLY PLYGLGELSQ240SFARLSAVYG GTYMLNKPID KIVVENGKVV GVMSEGKVAR CGKVICDPSY APDRVQKCGQ300VVRAICILNH AIPNVKNSHS LQIIIPHNQV NRCNDIYISC VSHLHHVCPE KFYLVLVATT360VETSNPHQEL QSGLQLLGHI EHIFYSIADL FEPVDDGKST NIFVSKSYDA STHFESTCTD420VLNIYERITG HPFDFSKVTR NLENE 445<210>33<211>487<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>33MVMDTTCSPM TERYLLSTYR HVLELFILIS MSVFGAKSVL KLFKKYKGEF LRAGFAGVDM 60NKPTKPTIPE AQGVICGVVF LSIMFLFIPV PFWRFLVRKA ETQSNEICSS DKIREGQEAF120FKSQFIQYLA GLLSVCCMLF LGFADDALNL PWRHKIGFPF VAGLPLLMVY LANEGTTRIA180VPVMFRGSLG GSVDIGVLYY IYMGLLTVFC TNSINIYAGI NGLEVGQAIV IGASLILFNL240IELMGYHWRV HLFSLYFLIP FLAVCWSLYR VNRYPAKVFV GDTFCYFAGM TFAVVGILGH300FSKTLLLFFL PQIINFLYST PQLFGLIPCP RHRLPRYDST DGLLHPSRVR FHPKSLSRAG360
QLVLSVFSHL GIIECKLCPD CIPNIPAALK TENVAPQAGH ENMSSDEEQI SERQRARSRS420PVHAKLPTEE IVEVDNLTVI NMMLRVLGPK NEQQTTNTLL LLQVFCSCLA FMIRYPLAWF480FFDVPAN 487<210>34<211>255<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>34MKKFMDSTNT KFNRAIQKTS EIIGRAEKTE FDDDFVALLK DAESTHEASK HIMEAIELWI 60NPCVLRKFDD VACKVESMVT DNKSYWLKVP AKLPAEHLGD TLVNAAMDVG VNTQYGSALK120HCGEYGREIA AIESRRNSVL EKNTLYPLHR FLAIEWPDIQ KERSHLESVR LDYDQLRSKV180KHNDSSDPET GKKLENAKDI LYKQLEVTRS KLQLVKSVNQ TNLVAIKELV AAQRNYFNEC240KQKTEELASH LEGLR 255<210>35<211>404<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>35MCLKVAPKVQ DPYANNPSHK AFTRAFLSQS TVDFGEDNFL ASPLGVLFTL GILLGSGGAQ 60GKTSYQIAKT MRLKSANFPW NISETQQEMK SLYKELSDSL TMENTTLDGN EVKVVRISTG120VFTQETYDVE SNFNQSIKND FGGELLTVDF TNQTNAAQDI NKWVNEHSNG LVEEFFTDDI180PKDAWMILIN IFYFRDYWEN PFVPYYTSLE NFSVTNNHHL QVPMMSKDEI LKYKKFQEDG240FEIVSKPMKN TRFSFIILLP LEKWNLNGAI EVLNGNKILS HYVDKLEETS VSLKMPKFTL300TKKLQLVGTL KSIGIKNLFD PLKADLSGIS SKNNLYVRSF IQTNVMKVNE SGIEAASVTS360PIIVPISALI PDVNFHVTHP FICFIYDQQL NMPLLAAKVI NPML 404<210>36<211>361<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>36MATRMSDMGM DVQINRRLNS KYDADSEAEA IGWLNQLTNE NVPFGRENVA AALKNGQILI 60KLINAVFDGT PTLPSSAAKM KRPLKANTMT APFKQMENIQ MFLNAAVAYG VPRASLFQTV120DLYELRNMPQ VLNTILQLGT ECQRNNFNGP VCGPKPTYEN KREFTEEQLR ASEGIIGLQA180GTNKCASQAG MSHGGPRHIT DIKVDAASKE SQGVIGLQMG SNKGASQAGM SHGGPRHITD240IKVDAASKES QSVIGLQMGS NKGASQAGMS HGGPRHITDI KVDAASKDGQ CVIGLQMGSN300KGASQAGMSH GGQRHIADIK VGDMSREGSN VIGLQMGTGK DQVASQSGMS FGAQRHIVDT360H361
<210>37<211>438<212>PRT<213>日本血吸蟲(chóng)(schistosoma japonicum)<400>37MQAPHRLLIM LALRLQVRRS LTRNISGWSD NLRKQSKLSE VVYSREDKYG AHNYHPLPVA 60LSKGKGIYVW DVEGNCYMDF LSAYSAVNQG HCHPRIVDAI KTQSETLTLT SRAFYNVVLG120EFEEMATKMF GYDKVLPMNS GVEAGETSLK LARKWGYKVK KIPDGQARVI FAEGNFWGRT180LAACSSSTDP ACYSGFGPFM PGFVIVPYDD LNALEVELKN PNTCAFMVEP IQGEAGVRTP240SDGYLKGVRK LCTKYNVLFI ADEIQTGLGR TGKRLCVNHE DVRPDIVLLG KALSGGMLPI300SAVLADDEVM LTIQPGEHGS TYGGNPLACR VAMAALRVLE EEGLAERAQK LGEIFRNELS360KLPKSVVELY RGKGLLNAIV IRQGFNAWDV CLKLRDHGLL AKPTHDTIIR LAPPLVIKED420ELHKAAEIMH KVIGSLSN 438<210>38<211>390<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>38MTTIESLTQE QWDDLFHNFE SLRESNNIAW SDLKRALEIV GVSVAGHEIR DLVGQPRNWL 60NLAEFNDLYM RAKDMKDTTK AIRKALLLKH SEDVKSFTVG KNDTDTRHSV SKAEERGFTL120WINKRLGHDT ELQNEILPID PSIDGQLYQR CKNGILLCKL VNVASPDTID ERSINRGAAL180KNVFNVHENL TLAVNSAASI GCCVVNTGPE DIMQGKRHIV LGLIWQLIRR GLVDTITLNK240HGELLALLHD GENAEDLAAM KPEELLMRWV NYHLHRAGCD RRITNFNSDL ADSVVYAHLM300EQIVPLDKRY NLMSADEILS STSRQERAIN VLNNAETLNT PFLLAPEDIY LAGDKDKENR360DRLNLAFLAT LFNMYPGLDT TRGDLLIEGE 390<210>39<211>472<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>39MVHQNRILQL FLFICTSFVS SSSIPWPPKE NYFDQTLDHF SFQARNLTFK QRYLYEDKWF 60KPNGPIFFYC GNEGEIGGFW NNTGLVFELA PSFNAFILFA EHRYYGKSLP FDKSFQQPYI120QYLSIGQALA DYAYLIEGIK SKFNMTRSPV VAFGGSYGGM LAAYMRAKYP HIVKGALAAS180APVRWVAGEG NFHDFFEAVT KDYHDADPKC SEKIKNAFTV AVQLSQKPDV GYKQLSEQLR240LCQPIQNDFE FYWMLKWARN AFVMMAMLDY PYKASFMASL PPNPVNVSCK NALSAIDPIP300TLREAVGVFY NSSQSLMCFD YKTQFIECAD ITGCGLGNDS LAWDFQSCTE MNLHDDSDST360TSDMFTSLPL TKQQVTSYCQ QKWGVTPAFN QLSTFFGDYI WKTASNIIFS NGNLDPWMGG420GILTDQSEKV ISLMLDGGAH HLDLRSPDPN DPPSARQIRQ IEVQTIRSWL DS472
<210>40<211>196<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>40MARCNLLKGI KRNSMTNIKS KMNILQLVKQ MDALSPRIQA LLKESNRFKS NEGKNNESQF 60SRESLKARLS DLEFRVTQNK ETEKAFSGKY VNEKSPGIYK CVVCSSKLFS SEAKFFCSCG120WPAFNSPIEY NCLVASIDLS SGDVRVEVSC KRCNAHLGHV FDDGPDPTGL RYCINSCALV180LDPKVGSSLD LNMPTT196<210>41<211>377<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>41MTMKNEIVVP PGLRELLQEL TVSILRERPD NLIQFAIDFL MMKKAASENR PNKANESDED 60EDEEPMPMPP QRVSRRAGVA AESYDPEKDD SSSAVKVVHP KTEEQRQRLT QATKDILLFR120CLDDDQMKDV IDAMFERHVS PGEKVITLGE DGDNFYVIEK GVYDIIVKVG DEEKVVGKYD180NKGSFGELAL MYNTPRAATI LAKTEGVLWA MTREVFRSIV LKKAFEKRRL YEELLNQVPI240LESLSAYERM SIADALRTKI FEANEQIIKQ GDPGDEMFFV EEGKVSIKMK RSGETEEKEV300AVIEKGGYFG ELALLTSHPR AASAYADCRT KLAVLDVGSF ERLLGPCLDI LRRNIDDYEA360KLKDIFGSLD KVPELRR 377<210>42<211>211<212>PRT<213>日本血吸蟲(chóng)(Schistosoma japonicum)<400>42MSSPVIGAVK SKMQGMKLQI DQLKQEVSSK QAVLRKEEEN KTKAEAEVAS LQKRIRQLED 60ELESTETRLQ EATLKLEEAS KAADESDRAR RVLEARQTAE DERILQLESM VQETAKSVKD120AETKYEEATR KLAVAEVALS HAEDRIEAAE SRLKELQSII HGTMGQLKSL EHQESQLSKQ180RSLHQSQLAS LSKQLIEAER RVKEASHQEE M 21權(quán)利要求
1.一類(lèi)日本血吸蟲(chóng)表皮蛋白，其特征在于，選自下組(a)具有SEQ ID No.22～SEQ ID No.42所示氨基酸序列的蛋白；(b)由(a)的蛋白發(fā)生一個(gè)或幾個(gè)氨基酸置換、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
2.如權(quán)利要求1所述的蛋白，其特征在于，它具有SEQ ID No.22～SEQ ID No.42所示的氨基酸序列。
3.一類(lèi)日本血吸蟲(chóng)表皮蛋白基因，其特征在于，它編碼權(quán)利要求1所述的蛋白。
4.如權(quán)利要求3所述的基因，其特征在于，選自下組(a)SEQ ID No.1～SEQ ID No.21所示的核苷酸序列；(b)SEQ ID No.1～SEQ ID No.21所示的核苷酸序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.21所示的核苷酸序列中每條序列有至少70％同源性、且編碼相同功能蛋白質(zhì)的核苷酸序列。
5.如權(quán)利要求3或4所述的基因，其特征在于，它具有SEQ ID No.1～SEQ ID No.21所示的核苷酸序列。
6.一類(lèi)日本血吸蟲(chóng)病防治疫苗，其特征在于，所述疫苗以權(quán)利要求1所述的蛋白為免疫原。
7.一類(lèi)能與權(quán)利要求1所述的蛋白特異性結(jié)合的抗體。
8.權(quán)利要求1所述的蛋白在制備預(yù)防和治療日本血吸蟲(chóng)病的藥物中的應(yīng)用。
全文摘要
本發(fā)明公開(kāi)了日本血吸蟲(chóng)(Schistosoma japonicum)表皮蛋白及其基因序列，還公開(kāi)了與該蛋白特異結(jié)合的抗體，以及把表皮蛋白作為免疫原制備所得的日本血吸蟲(chóng)病防治疫苗，此外，本發(fā)明還公開(kāi)了日本血吸蟲(chóng)表皮蛋白在制備預(yù)防和治療日本血吸蟲(chóng)病的藥物中的應(yīng)用。運(yùn)用本發(fā)明的日本血吸蟲(chóng)表皮蛋白，可研究血吸蟲(chóng)治療藥物的作用靶點(diǎn)，從而在開(kāi)發(fā)研制預(yù)防或治療血吸蟲(chóng)病的藥物過(guò)程中發(fā)揮重要作用。
文檔編號(hào)A61P33/12GK1986811SQ20051011173
公開(kāi)日2007年6月27日 申請(qǐng)日期2005年12月20日 優(yōu)先權(quán)日2005年12月20日
發(fā)明者韓澤廣, 劉鋒, 王志勤, 胡薇, 馮正 申請(qǐng)人:上海人類(lèi)基因組研究中心, 中國(guó)疾病預(yù)防控制中心寄生蟲(chóng)病預(yù)防控制所