殘基被構(gòu)筑在提議的Qc傳 輸通道的開口附近，所W將運(yùn)些殘基W龐大的苯丙胺酸替換，預(yù)期會阻礙或阻斷通過通道 的PQ分子的擴(kuò)散。然而，我們并沒有在運(yùn)些突變細(xì)胞中看到運(yùn)些作用。因此，我們的研究 結(jié)果不符合那些提議的Qe-位置，或者Qe傳輸通道可能在從藍(lán)綠藻中的池的Qe位置上的PQ的交換中，扮演重要的角色巧]。
[0111] 3. 2細(xì)胞色素b559與PsbJ設(shè)及藍(lán)光誘導(dǎo)的NPQ機(jī)制的意義
[0112] 細(xì)胞色素bggg的細(xì)胞質(zhì)側(cè)位于PSII內(nèi)與藻膽體的APC核屯、復(fù)合物的預(yù)測接觸位點 巧8]。另外，我們先前的研究表明，在細(xì)胞色素bssg的細(xì)胞質(zhì)側(cè)上的R7La與R1化0突變 對0CP誘導(dǎo)的NPQ及其黑暗恢復(fù)具有顯著的抑制效果巧2]。因此，我們建議，細(xì)胞色素bwe 與PsbJ上的突變可能影響在藍(lán)綠藻中0CP誘導(dǎo)的NPQ，大概是通過改變在APC核屯、復(fù)合物 與該0CP之間的相互作用。我們的研究結(jié)果還表明，細(xì)胞色素beee與PsbJ的細(xì)胞質(zhì)側(cè)可能 有助于或穩(wěn)定在PSII中APC核屯、復(fù)合物與0CP的停靠點。該建議也與近期通過使用原生 的電噴霧質(zhì)譜和蛋白質(zhì)交聯(lián)的組合來研究0CP的結(jié)構(gòu)結(jié)合位點的研究結(jié)果一致巧9]。
[0113] 3. 3運(yùn)些突變細(xì)胞對生物能源生產(chǎn)的應(yīng)用
[0114] 在我們的正常生長條件下，相較于野生型細(xì)胞，A16FJ與V32F0突變細(xì)胞顯示出 顯著較高的光合生長速率（約1. 1倍）W及增加的生質(zhì)積累（約30-40% )。因為運(yùn)些突 變細(xì)胞表現(xiàn)出對0CP誘導(dǎo)的NPQ機(jī)制的嚴(yán)重抑制，因此上述效果可W歸因于自其藻膽體浪 費能量耗散的降低，從而相較于野生型細(xì)胞，提高其光合效率。運(yùn)些突變細(xì)胞改進(jìn)的光合作 用和生長特性可有實際的應(yīng)用，W提高在藍(lán)綠藻中生質(zhì)和生物燃料的生產(chǎn)[40-42]。我們 的FT-IR分析顯示脂質(zhì)、蛋白質(zhì)及糖類含量于A16FJ與V32Fβ突變細(xì)胞含量較高，相較于 野生型細(xì)胞在正常生長條件下。
[0115] 4.結(jié)論
[0116] 我們的研究結(jié)果表明，數(shù)個在細(xì)胞色素bw9與PsbJ上點突變的集胞藻PCC6803突 變細(xì)胞顯現(xiàn)出在中度藍(lán)光條件下狀態(tài)轉(zhuǎn)換的提升作用W及在強(qiáng)度藍(lán)光條件下藍(lán)光誘發(fā)的 非光化學(xué)巧光澤焰（no噸hotochemicalfluorescencequenching,NP曲的減弱作用。我們 的結(jié)果顯示PSII的細(xì)胞色素bsee與PsbJ上調(diào)節(jié)藍(lán)綠藻的狀態(tài)轉(zhuǎn)換及藍(lán)光誘發(fā)的非光化學(xué) 巧光澤焰。此外，在正常生長條件下，相較于野生型細(xì)胞，運(yùn)些突變細(xì)胞中有一些表現(xiàn)出增 加的光合生長速率與生質(zhì)生產(chǎn)。運(yùn)些突變細(xì)胞可具有用于增加生質(zhì)與生物能源生產(chǎn)的產(chǎn)率 的實際應(yīng)用。
[0117] 序列信息
[0118] 野生型細(xì)胞色素b559a多勝膚（SEQIDN0:1)
[0119]SGTTGERPFSDIVTSIRYWVI唯ITIPMLFIAGWLFVSTGLAYDAFGTPRPDEYFTQTR犯LPIL犯RY DIN犯W邸NQ
[0120] 野生型細(xì)胞色素b559 0多勝膚（SEQIDNO: 2)
[0121] ATQNPNQPVTYPIFTVRWLAVHTLAVPSVFFYGAIAAMQ門QR
[0122] 野生型細(xì)胞色素PsbJ多勝膚（SEQIDNO:3)
[0123]MFAEGRIPLWVVGVVAGIGAIGVLGLFFYGAYAGLGSSM
[0124] 突變型細(xì)胞色素b559a多勝膚（SEQIDNO:4)
[01 巧]SGTTGERPFSDIVTSIRYWVIHAITIPMLFIAGWLFVSTGLAYDAFGTPRPDEYFTQTR犯LPIL犯RY DIN犯W邸NQ
[0126] 突變型細(xì)胞色素b559 0多勝膚（SEQIDNO: 5)
[0127]ATQNPNQPVTYPIFTVRWLAVHTLAVPAVFFVGAIAAMQ門QR
[012引 突變型細(xì)胞色素b559 0多勝版（SEQ ID N0:6)
[0129] ATQNPNQPVTYPIFTVRWLAVHTLAVPYVFFVGAIAAMQ門Q民
[0130]突變型細(xì)胞色素b559 0多勝版（SEQIDN0:7)
[0131] ATQNPNQPVTYPIFTVRWLAVHTLAVPSVFFEGAIAAMQ門Q民
[0132]突變型細(xì)胞色素PsbJ多勝版（SEQIDN0:8)
[0133] MFAEGRIPLWVVGVVFGIGAIGVLGLFFYGAYAGLGSSM
[0134]突變型細(xì)胞色素PsbJ多勝版（SEQIDN0:9)
[0135] MFAEGRIPLWVVGVVRGIGAIGVLGLFFYGAYAGLGSSM
[0136]突變型細(xì)胞色素PsbJ多勝版（SEQ ID NO: 10)
[0137]MFAEGRIPLWVVGVVAGI化IGVLGLFFYGAYAGLGSSM
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