gca ctg age ttg gca ate ttg gag tct att att ggt gag cat ggc687
Ala Ala Leu Ser Leu Ala lie Leu Glu Ser lie lie Gly Glu His Gly 190195200
gtc tct cct atg gcg aaa cag age ctg gca ctg cga ttg tcg age att735
Val Ser Pro Met Ala Lys Gln Ser Leu Ala Leu Arg Leu Ser Ser lie205210215
atg cgt gaa gtt cat gat gtg gag aaa gcc aag ggg gag caa act gta783
Met Arg Glu Val His Asp Val Glu Lys Ala Lys Gly Glu Gln Thr Val220225230235
tgt caa gtc caa gat gtg gag aaa gcc gag ggg aag caa act gtg agt831
Cys Gln Val Gln Asp Val Glu Lys Ala Glu Gly Lys Gln Thr Val Ser240245250
gaa gtc cat gat gtg aag aaa gac aag aaa gtc gag ggg gag caa act879
Glu Val His Asp Val Lys Lys Asp Lys Lys Val Glu Gly Glu Gln Thr255260265
gtg agt act gaa gtg cat gat gag gag aaa tga tgcggggcca cgaagtttga 932Val Ser Thr Glu Val His Asp Glu Glu Lys270275
ggg935
〈210〉 2〈211〉 277〈212〉 PRT<213> Freesia alba〈400〉 2
Met Gly Glu Ala Ser Pro Leu Ala Ser Ala Thr Thr Lys Leu Glu Val151015
Glu Gly Val Val Phe Gln Pro His Leu Thr Pro Pro Gly Ser Ser Lys202530
Pro His Phe Leu Gly Gly Ala Gly Val Arg Gly Leu Glu lie Gly Gly 354045
Lys Phe lie Ser Phe Thr Ala lie Gly lie Tyr Leu Glu Ser Glu Thr505560Ala Val Lys Ala Leu Ala Thr Gln Trp Lys Gly Lys Ala Ser Glu Glu65707580
Leu Thr Ser Cys Ala Asp Phe Phe Arg Asp lie Tyr Thr Gly Pro Phe859095
Glu Lys Phe Thr Arg Val Thr Met lie Leu Pro Leu Thr Gly Gln Gln100105110
Tyr Thr Glu Lys Val Thr Glu Asn Cys Val Ala Tyr Trp Lys Ala lie 115120125
Gly lie Tyr Thr Asp Ala Glu Ala Ala Ala Val Asp Lys Phe Lys Glu130135140
Val Phe Lys Ser Glu Ser Phe Ala Pro Gly Ser Ser lie Leu Phe Ser145150155160
His Ser Pro Ser Gly Ser Leu Thr lie Ala Phe Ser Lys Asp Glu Ser165170175
lie Pro Glu Ala Gly Thr Ala Val lie Glu Asn Ala Ala Leu Ser Leu180185190
Ala lie Leu Glu Ser lie lie Gly Glu His Gly Val Ser Pro Met Ala 195200205
Lys Gln Ser Leu Ala Leu Arg Leu Ser Ser lie Met Arg Glu Val His210215220
Asp Val Glu Lys Ala Lys Gly Glu Gln Thr Val Cys Gln Val Gln Asp225230235240
Val Glu Lys Ala Glu Gly Lys Gln Thr Val Ser Glu Val His Asp Val245250255
Lys Lys Asp Lys Lys Val Glu Gly Glu Gln Thr Val Ser Thr Glu Val260265270
His Asp Glu Glu Lys275o
【主權(quán)項】
1.控制香雪蘭花色苷合成的查爾酮異構(gòu)酶的CDNA，其特征是: 其中:香雪蘭基因的全長cDNA序列(SEQ ID N0.1)TCTAATGACAACAACAACGCTCCAGCCATCGGCTCTGGTCATAACCAAACTGGTGATCGCCGGCTTCCTCTTCCCTCCGGCGATCACACCGCCGCCCGGCGATCAGCCCACACTATTCCTCGCCGGCGCAGGACAGAGGGGATTGGATGTGGGGGGCAAACTCGTAATTTTTACGGCCATCGGCGTGTACCTGGAGGAGTCGGCGGTCCCGTCACTCGCCGGTAAATGGGAGGGGAAGGCGGCGGAGGAGCTCCTCGTGTCCGATCATTTCTTCCGGGATATCTACACCGGTCCATTTGAGAGGCTCACAAAGGTGACCATGGTCTTGCCACTGACGGGCAAAGAGTACTCGGATAAGGTGTCCGAGAACTGTGTGGCCTACTGGAAGGCTGTCGGGACCTACGACAGAGCTAAACACGCGGCGATAGTGAAGCTAAAAGAGGTCTTTAAGTCCAAGACCTTTTCTCATGGCGCATCGATCCTCTTCACTCACGAATCTTCTGGCTCTTTGACTATTGGATTTTTGAAGAACAACTCGATGCCCGATTCTGGGGGCACAGTTATAAGGAGTAAAGCTCTGTCGGAGGCGATCTTGGAGTCTATCATTGGCAAGAATGGAGTGTCGCCTGCAGCGAAAGAAAGTTTGGCGAGACGCATTTCAGCACTAATGAAGGAAAGGATCACGTCGTMGAA, 其中:由FhCHIl基因核苷酸序列推測得到的蛋白質(zhì)序列(SEQ ID N0.2)MTTTTLQPSALVITKLVIAGFLFPPAITPPP⑶QPTLFLAGAGQRGLDVGGKLVIFTAIGVYLEESAVPSLAGKWEGKAAEELLVSDHFFRDIYTGPFERLTKVTMVLPLTGKEYSDKVSENCVAYWKAVGTYDRAKHAAIVKLKEVFKSKTFSHGASILFTHESSGSLTIGFLKNNSMPDSGGTVIRSKALSEAILESIIGKNGVSPAAKESLARRISALMKERITS-， 開放閱讀框架687bp，編碼228個氨基酸，蛋白質(zhì)的分子量推測是24，417Da，等電點為.9.23 ； 其中'FhCHI2基因的全長cDNA序列(SEQ ID N0.3)CTGCAAATGCCACAAAAGTAAAGGCAAAGCCAACCAATCCTCACACTCTTTGTTATAACTCCCTCAACAACCAAACTCACAAGCATCACTAGTTTCTGATGACAATGGTGGAGATGGAAGTGTCACATTTGGTTTCAGCAACAACCACAAAGCAAGAAATGCCTTTGGTTTCAGCAACAACCAAATTAGAAGTGGAAGGAGTTCTCTTCCAACCTCACCTCACACCATCTGGTTCTCCCCATAATCATTTTCTCGCCGGTGCCGGTGTGAGAGGATTGGAGATTGGTGGCAAGTTCATAAGCTTTACCGCGATCGGCATCTACTTAGAAACTGAAGCAGCCGTCGAAGCCCTCGCTGTCAAATGGAAGGGCAAGACTGCCGAGGAGCTTGCGGCATCTGCCGACTTCTTTCGAGATATCTACACAGGACCATTTGAGAAATTCACTAGAGTGACAATGATTCTCCCACTGACCGGCCAGCAGTACGCCGAGAAGGTGACCGAGAACTGCGTCGCTTACTGGAAGGCCATCGGTAAATATACAGACGCAGAAGCTGCAGCTGTCGACAAGTTCAGCGAAGTCTTCAAGCCGGAGATTTTCCCTCCTGGCTCCTCCATCCTCTTCACTCATTCCCCATCTGGTTCGCTGACGATTGCCTTCTCCAAGGATGACTCCATTCCTGAAGCTGGCACTGCGATTATCGATAACGCAATGCTGACTGTGGCAATCTTGGAGTCGATCATCGGCGAACATGGCGTCTCTCCTGCAGCGAAACAGAGCCTGGCGGTGCGACTGTCGAGCATGCTGAGTGAAGTCCAAGATGTGGAGAAATCTCAGGGCGAGAAAACTGTGACTGTAATGTGTGCATGATATGGAGAGGCAGAGAGAAAAAGAAGCAGCCTTTGTAATCCTAAGGGTTCGGATTATTAAAATAGGGAAATAAAAAGAGTTTGTAAGCATCTTGATTTCAAATATTATATATTGATTTTGTAATTTAAGCAAAAAAAAAAAAAAAAAAAAAAAA， 其中:由基因核苷酸序列推測得到的蛋白質(zhì)序列(SEQ ID N0.4)MTMVEMEVSHLVSATTTKQEMPLVSATTKLEVEGVLFQPHLTPSGSPHNHFLAGAGVRGLEIGGKFISFTAIGIYLETEAAVEALAVKWKGKTAEELAASADFFRDIYTGPFEKFTRVTMILPLTGQQYAEKVTENCVAYWKAIGKYTDAEAAAVDKFSEVFKPEIFPPGSSILFTHSPSGSLTIAFSKDDSIPEAGTAIIDNAMLTVAILESIIGEHGVSPAAKQSLAVRLSSMLSEVQDVEKSQGEKTVTVMCA-, 開放閱讀框架長771bp，編256個氨基酸，蛋白質(zhì)的分子量推測是27，439Da，等電點為.5.03 ； 其中基因的cDNA全長核苷酸序列(SEQ ID N0.5)CCCTTTCCTTATATATAGGCAAACGCAAAGTTCGACCGAGTCACTCTCTTTTTTGTTGCAACTCCCTTTCTGCTCAAAATGGGCGAGGCATCACCTCTGGCTTCTGCAACAACCAAGCTGGAAGTGGAAGGAGTTGTCTTCCAACCTCACCTCACACCTCCTGGCTCTTCCAAGCCTCATTTCCTCGGCGGAGCCGGTGTGAGGGGATTGGAGATTGGTGGAAAGTTCATAAGCTTCACAGCGATCGGCATTTACTTGGAGAGCGAGACGGCCGTTAAGGCCCTCGCTACCCAATGGAAGGGCAAGGCTTCCGAGGAGCTGACATCCTGTGCGGACTTCTTTCGGGATATCTACACAGGACCATTTGAGAAGTTCACTAGAGTGACAATGATCCTCCCGCTGACCGGCCAGCAGTACACCGAGAAGGTGACCGAGAACTGCGTTGCCTACTGGAAGGCCATCGGTATCTATACCGACGCAGAAGCCGCAGCTGTAGACAAGTTCAAGGAAGTCTTCAAGTCAGAGAGCTTCGCTCCTGGCTCCTCCATCCTCTTCAGTCACTCCCCATCTGGTTCGCTGACGATTGCATTCTCCAAGGATGAGTCCATTCCTGAAGCCGGTACTGCAGTAATCGAGAATGCGGCACTGAGCTTGGCAATCTTGGAGTCTATTATTGGTGAGCATGGCGTCTCTCCTATGGCGAAACAGAGCCTGGCACTGCGATTGTCGAGCATTATGCGTGAAGTTCATGATGTGGAGAAAGCCAAGGGGGAGCAAACTGTATGTCAAGTCCAAGATGTGGAGAAAGCCGAGGGGAAGCAAACTGTGAGTGAAGTCCATGATGTGAAGAAAGACAAGAAAGTCGAGGGGGAGCAAACTGTGAGTACTGAAGTGCATGATGAGGAGAAATGATGCGGGGCCACGAAGTTTGAGGG， 其中:由基因核苷酸序列推測得到的蛋白質(zhì)序列(SEQ ID N0.6)MGEASPLASATTKLEVEGVVFQPHLTPPGSSKPHFLGGAGVRGLEIGGKFISFTAIGIYLESETAVKALATQffKGKASEELTSCADFFRDIYTGPFEKFTRVTMILPLTGQQYTEKVTENCVAYffKAIGIYTDAEAAAVDKFKEVFKSESFAPGSSILFSHSPSGSLTIAFSKDESIPEAGTAVIENAALSLAILESIIGEHGVSPMAKQSLALRLSSIMREVHDVEKAKGEQTVCQVQDVEKAEGKQTVSEVHDVKKDKKVEGEQTVSTEVHDEEK-, 開放閱讀框架長834bp，編碼277個氨基酸，蛋白質(zhì)的分子量推測是29，832Da，等電點為 5.14。
【專利摘要】本發(fā)明首次從紅花香雪蘭花瓣中克隆得到3條查爾酮異構(gòu)酶（CHI）的cDNA，并確定了它的核苷酸序列和由此得出氨基酸序列。在香雪蘭花朵著色過程中，三者基因表達(dá)和花色苷合成呈現(xiàn)明顯的相關(guān)性，另外，將3個<i>FhCHI</i>的真核表達(dá)載體轉(zhuǎn)染到擬南芥<i>CHI</i>突變體中，其幼苗和種皮都恢復(fù)了表型。
【IPC分類】C12N15/61, C12N9/90
【公開號】CN105112434
【申請?zhí)枴緾N201510403545
【發(fā)明人】王麗, 梁瓴婕, 高翔
【申請人】東北師范大學(xué)
【公開日】2015年12月2日
【申請日】2015年7月12日